UTHEALTH HOME    ABOUT SBMI    A-Z    WEBMAIL    INSIDE THE UNIVERSITY
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use

Center for Computational Systems Medicine level1
leaf

Fusion Gene Summary

leaf

Fusion Gene Sample Information

leaf

Fusion ORF Analysis

leaf

Fusion Amino Acid Sequences

leaf

Fusion Protein Functional Features

leaf

Fusion Protein-Protein Interaction

leaf

Related drugs with this fusion protein

leaf

Related disease with this fusion protein

Fusion Protein:WASF2-FGR

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: WASF2-FGR
FusionPDB ID: 98726
FusionGDB2.0 ID: 98726
HgeneTgene
Gene symbol

WASF2

FGR

Gene ID

10163

2268

Gene nameWASP family member 2FGR proto-oncogene, Src family tyrosine kinase
SynonymsIMD2|SCAR2|WASF4|WAVE2|dJ393P12.2SRC2|c-fgr|c-src2|p55-Fgr|p55c-fgr|p58-Fgr|p58c-fgr
Cytomap

1p36.11

1p35.3

Type of geneprotein-codingprotein-coding
Descriptionwiskott-Aldrich syndrome protein family member 2WAS protein family member 2WASP family Verprolin-homologous protein 2WASP family protein member 2WASP family protein member 4protein WAVE-2putative Wiskott-Aldrich syndrome protein family member 4supptyrosine-protein kinase FgrGardner-Rasheed feline sarcoma viral (v-fgr) oncogene homologc-fgr protooncogenec-src-2 proto-oncogenefeline Gardner-Rasheed sarcoma viral oncogene homologp55-c-fgr proteinproto-oncogene c-Fgrproto-oncogene tyrosine-prote
Modification date2020031320200329
UniProtAcc.

P09769

Ensembl transtripts involved in fusion geneENST idsENST00000430629, ENST00000536657, 
ENST00000468038, ENST00000374005, 
ENST00000399173, ENST00000545953, 
ENST00000374004, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score11 X 7 X 10=7706 X 7 X 6=252
# samples 2310
** MAII scorelog2(23/770*10)=-1.74322458463789
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(10/252*10)=-1.33342373372519
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: WASF2 [Title/Abstract] AND FGR [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)WASF2(27816497)-FGR(27951662), # samples:15
Anticipated loss of major functional domain due to fusion event.WASF2-FGR seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
WASF2-FGR seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneFGR

GO:0018108

peptidyl-tyrosine phosphorylation

7519620|8327512

TgeneFGR

GO:0046777

protein autophosphorylation

2181286|8327512


check buttonFusion gene breakpoints across WASF2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across FGR (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-BH-A1FD-01AWASF2chr1

27755271

-FGRchr1

27939861

-
ChimerDB4ESCATCGA-L5-A891-01AWASF2chr1

27816497

-FGRchr1

27951662

-
ChimerDB4ESCATCGA-L5-A891WASF2chr1

27816496

-FGRchr1

27951662

-
ChimerDB4ESCATCGA-L5-A891WASF2chr1

27816497

-FGRchr1

27951662

-
ChimerDB4ESCATCGA-L5-A891WASF2chr1

27816497

-FGRchr1

27967635

-
ChimerDB4LUSCTCGA-66-2759-01AWASF2chr1

27816497

-FGRchr1

27951662

-
ChimerDB4LUSCTCGA-66-2759WASF2chr1

27816497

-FGRchr1

27951662

-
ChimerDB4LUSCTCGA-66-2759WASF2chr1

27816497

-FGRchr1

27967635

-
ChimerDB4OVTCGA-13-0893-01BWASF2chr1

27816497

-FGRchr1

27951662

-
ChimerDB4OVTCGA-23-2078-01AWASF2chr1

27816497

-FGRchr1

27951662

-
ChimerDB4OVTCGA-23-2078WASF2chr1

27816496

-FGRchr1

27951662

-
ChimerDB4SKCMTCGA-ER-A19W-06AWASF2chr1

27816497

-FGRchr1

27951662

-
ChimerDB4SKCMTCGA-ER-A19W-06AWASF2chr1

27816497

-FGRchr1

27967635

-
ChimerDB4UCSTCGA-NA-A4R1-01AWASF2chr1

27816494

-FGRchr1

27951659

-
ChimerDB4UCSTCGA-NA-A4R1-01AWASF2chr1

27816497

-FGRchr1

27951662

-
ChimerDB4UCSTCGA-NA-A4R1WASF2chr1

27816496

-FGRchr1

27951662

-
ChimerDB4UCSTCGA-NA-A4R1WASF2chr1

27816497

-FGRchr1

27951662

-


Top

Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000430629WASF2chr127755271-ENST00000374005FGRchr127939861-1537346216686156
ENST00000430629WASF2chr127755271-ENST00000399173FGRchr127939861-1309346216686156
ENST00000430629WASF2chr127755271-ENST00000545953FGRchr127939861-1309346216686156
ENST00000430629WASF2chr127755271-ENST00000374004FGRchr127939861-1301346216686156
ENST00000536657WASF2chr127755271-ENST00000374005FGRchr127939861-1430239109579156
ENST00000536657WASF2chr127755271-ENST00000399173FGRchr127939861-1202239109579156
ENST00000536657WASF2chr127755271-ENST00000545953FGRchr127939861-1202239109579156
ENST00000536657WASF2chr127755271-ENST00000374004FGRchr127939861-1194239109579156

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000430629ENST00000374005WASF2chr127755271-FGRchr127939861-0.0234852490.9765147
ENST00000430629ENST00000399173WASF2chr127755271-FGRchr127939861-0.0280497780.97195023
ENST00000430629ENST00000545953WASF2chr127755271-FGRchr127939861-0.0280497780.97195023
ENST00000430629ENST00000374004WASF2chr127755271-FGRchr127939861-0.0299975610.9700024
ENST00000536657ENST00000374005WASF2chr127755271-FGRchr127939861-0.0439588840.95604116
ENST00000536657ENST00000399173WASF2chr127755271-FGRchr127939861-0.035669350.9643306
ENST00000536657ENST00000545953WASF2chr127755271-FGRchr127939861-0.035669350.9643306
ENST00000536657ENST00000374004WASF2chr127755271-FGRchr127939861-0.0391026180.9608974

Top

Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>98726_98726_1_WASF2-FGR_WASF2_chr1_27755271_ENST00000430629_FGR_chr1_27939861_ENST00000374004_length(amino acids)=156AA_BP=37
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMN

--------------------------------------------------------------

>98726_98726_2_WASF2-FGR_WASF2_chr1_27755271_ENST00000430629_FGR_chr1_27939861_ENST00000374005_length(amino acids)=156AA_BP=37
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMN

--------------------------------------------------------------

>98726_98726_3_WASF2-FGR_WASF2_chr1_27755271_ENST00000430629_FGR_chr1_27939861_ENST00000399173_length(amino acids)=156AA_BP=37
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMN

--------------------------------------------------------------

>98726_98726_4_WASF2-FGR_WASF2_chr1_27755271_ENST00000430629_FGR_chr1_27939861_ENST00000545953_length(amino acids)=156AA_BP=37
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMN

--------------------------------------------------------------

>98726_98726_5_WASF2-FGR_WASF2_chr1_27755271_ENST00000536657_FGR_chr1_27939861_ENST00000374004_length(amino acids)=156AA_BP=37
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMN

--------------------------------------------------------------

>98726_98726_6_WASF2-FGR_WASF2_chr1_27755271_ENST00000536657_FGR_chr1_27939861_ENST00000374005_length(amino acids)=156AA_BP=37
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMN

--------------------------------------------------------------

>98726_98726_7_WASF2-FGR_WASF2_chr1_27755271_ENST00000536657_FGR_chr1_27939861_ENST00000399173_length(amino acids)=156AA_BP=37
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMN

--------------------------------------------------------------

>98726_98726_8_WASF2-FGR_WASF2_chr1_27755271_ENST00000536657_FGR_chr1_27939861_ENST00000545953_length(amino acids)=156AA_BP=37
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSSKFPIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMN

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:27816497/chr1:27951662)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.FGR

P09769

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. Together with CLNK, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production (By similarity). {ECO:0000250|UniProtKB:P14234, ECO:0000269|PubMed:10739672, ECO:0000269|PubMed:17164290, ECO:0000269|PubMed:1737799, ECO:0000269|PubMed:7519620}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneWASF2chr1:27755271chr1:27939861ENST00000430629-29268_27143.333333333333336499.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000430629-29318_32843.333333333333336499.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000430629-29333_33843.333333333333336499.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000430629-29347_35043.333333333333336499.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000430629-29364_37143.333333333333336499.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000430629-29379_38243.333333333333336499.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000430629-29391_40643.333333333333336499.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000536657-28268_27143.333333333333336282.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000536657-28318_32843.333333333333336282.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000536657-28333_33843.333333333333336282.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000536657-28347_35043.333333333333336282.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000536657-28364_37143.333333333333336282.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000536657-28379_38243.333333333333336282.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000536657-28391_40643.333333333333336282.0Compositional biasNote=Poly-Pro
HgeneWASF2chr1:27755271chr1:27939861ENST00000430629-29436_45343.333333333333336499.0DomainWH2
HgeneWASF2chr1:27755271chr1:27939861ENST00000536657-28436_45343.333333333333336282.0DomainWH2
TgeneFGRchr1:27755271chr1:27939861ENST00000374004811144_241416.3333333333333530.0DomainSH2
TgeneFGRchr1:27755271chr1:27939861ENST00000374004811263_516416.3333333333333530.0DomainProtein kinase
TgeneFGRchr1:27755271chr1:27939861ENST0000037400481177_138416.3333333333333530.0DomainSH3
TgeneFGRchr1:27755271chr1:27939861ENST000003740051013144_241416.3333333333333530.0DomainSH2
TgeneFGRchr1:27755271chr1:27939861ENST000003740051013263_516416.3333333333333530.0DomainProtein kinase
TgeneFGRchr1:27755271chr1:27939861ENST00000374005101377_138416.3333333333333530.0DomainSH3
TgeneFGRchr1:27755271chr1:27939861ENST000003991731013144_241416.3333333333333530.0DomainSH2
TgeneFGRchr1:27755271chr1:27939861ENST000003991731013263_516416.3333333333333530.0DomainProtein kinase
TgeneFGRchr1:27755271chr1:27939861ENST00000399173101377_138416.3333333333333530.0DomainSH3
TgeneFGRchr1:27755271chr1:27939861ENST00000374004811269_277416.3333333333333530.0Nucleotide bindingATP
TgeneFGRchr1:27755271chr1:27939861ENST000003740051013269_277416.3333333333333530.0Nucleotide bindingATP
TgeneFGRchr1:27755271chr1:27939861ENST000003991731013269_277416.3333333333333530.0Nucleotide bindingATP


Top

Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
WASF2
FGR


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs to WASF2-FGR


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to WASF2-FGR


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource