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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:DDIT3-ATF2 (FusionGDB2 ID:HG1649TG1386)

Fusion Gene Summary for DDIT3-ATF2

check button Fusion gene summary
Fusion gene informationFusion gene name: DDIT3-ATF2
Fusion gene ID: hg1649tg1386
HgeneTgene
Gene symbol

DDIT3

ATF2

Gene ID

1649

1386

Gene nameDNA damage inducible transcript 3activating transcription factor 2
SynonymsAltDDIT3|C/EBPzeta|CEBPZ|CHOP|CHOP-10|CHOP10|GADD153CRE-BP1|CREB-2|CREB2|HB16|TREB7
Cytomap('DDIT3')('ATF2')

12q13.3

2q31.1

Type of geneprotein-codingprotein-coding
DescriptionDNA damage-inducible transcript 3 proteinC/EBP zetaCCAAT/enhancer-binding protein homologous proteinalternative DDIT3 proteinc/EBP-homologous protein 10growth arrest and DNA damage-inducible protein GADD153cyclic AMP-dependent transcription factor ATF-2activating transcription factor 2 splice variant ATF2-var2cAMP response element-binding protein CRE-BP1cAMP responsive element binding protein 2, formerlycAMP-dependent transcription factor ATF-2cyclic A
Modification date2020031320200322
UniProtAcc

P0DPQ6

.
Ensembl transtripts involved in fusion geneENST00000346473, ENST00000547303, 
ENST00000551116, ENST00000552740, 
Fusion gene scores* DoF score5 X 11 X 4=2201 X 1 X 1=1
# samples 111
** MAII scorelog2(11/220*10)=-1
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(1/1*10)=3.32192809488736
Context

PubMed: DDIT3 [Title/Abstract] AND ATF2 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointDDIT3(57914201)-ATF2(175986270), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneDDIT3

GO:0000122

negative regulation of transcription by RNA polymerase II

18940792

HgeneDDIT3

GO:0006986

response to unfolded protein

18940792

HgeneDDIT3

GO:0007050

cell cycle arrest

22496745

HgeneDDIT3

GO:0032792

negative regulation of CREB transcription factor activity

8622660

HgeneDDIT3

GO:0034976

response to endoplasmic reticulum stress

18940792|19061639|20829347

HgeneDDIT3

GO:0042789

mRNA transcription by RNA polymerase II

14667815

HgeneDDIT3

GO:0043161

proteasome-mediated ubiquitin-dependent protein catabolic process

17872950

HgeneDDIT3

GO:0043433

negative regulation of DNA-binding transcription factor activity

16434966

HgeneDDIT3

GO:0045454

cell redox homeostasis

14667815

HgeneDDIT3

GO:0045892

negative regulation of transcription, DNA-templated

18940792

HgeneDDIT3

GO:0045893

positive regulation of transcription, DNA-templated

15322075|15775988

HgeneDDIT3

GO:0045944

positive regulation of transcription by RNA polymerase II

22065586

HgeneDDIT3

GO:0070059

intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress

18940792|20829347

HgeneDDIT3

GO:1903026

negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding

8622660

HgeneDDIT3

GO:1990440

positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress

15322075|24939851

HgeneDDIT3

GO:2000016

negative regulation of determination of dorsal identity

16434966

TgeneATF2

GO:0006357

regulation of transcription by RNA polymerase II

19861239

TgeneATF2

GO:0006970

response to osmotic stress

21098032

TgeneATF2

GO:0006974

cellular response to DNA damage stimulus

22304920

TgeneATF2

GO:0045944

positive regulation of transcription by RNA polymerase II

16300731|18671972

TgeneATF2

GO:0110024

positive regulation of cardiac muscle myoblast proliferation

22982025



check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/ABU662818DDIT3chr12

57914201

-ATF2chr2

175986270

-


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Fusion Gene ORF analysis for DDIT3-ATF2

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5UTR-intronENST00000346473ENST00000264110DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000345739DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000392543DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000392544DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000409437DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000409499DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000409635DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000409833DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000413123DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000426833DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000487334DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000346473ENST00000538946DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000264110DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000345739DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000392543DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000392544DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000409437DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000409499DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000409635DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000409833DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000413123DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000426833DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000487334DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000547303ENST00000538946DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000264110DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000345739DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000392543DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000392544DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000409437DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000409499DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000409635DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000409833DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000413123DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000426833DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000487334DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000551116ENST00000538946DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000264110DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000345739DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000392543DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000392544DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000409437DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000409499DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000409635DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000409833DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000413123DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000426833DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000487334DDIT3chr12

57914201

-ATF2chr2

175986270

-
5UTR-intronENST00000552740ENST00000538946DDIT3chr12

57914201

-ATF2chr2

175986270

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for DDIT3-ATF2


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


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Fusion Protein Features for DDIT3-ATF2


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:57914201/:175986270)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
DDIT3

P0DPQ6

.
FUNCTION: [Isoform AltDDIT3]: Product of the upstream open reading frame (uORF) of DDIT3/CHOP that is specifically produced in absence of stress, thereby preventing translation of downstream stress effector DDIT3/CHOP. {ECO:0000250|UniProtKB:A0A2R8VHR8}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for DDIT3-ATF2


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for DDIT3-ATF2


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for DDIT3-ATF2


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for DDIT3-ATF2


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneDDIT3C0206634Liposarcoma, Myxoid3CTD_human;ORPHANET
HgeneDDIT3C0003949Asbestosis1CTD_human
HgeneDDIT3C0006142Malignant neoplasm of breast1CTD_human
HgeneDDIT3C0011616Contact Dermatitis1CTD_human
HgeneDDIT3C0011854Diabetes Mellitus, Insulin-Dependent1CTD_human
HgeneDDIT3C0015695Fatty Liver1CTD_human
HgeneDDIT3C0017614Glaucoma, Suspect1CTD_human
HgeneDDIT3C0021831Intestinal Diseases1CTD_human
HgeneDDIT3C0027051Myocardial Infarction1CTD_human
HgeneDDIT3C0027720Nephrosis1CTD_human
HgeneDDIT3C0028754Obesity1CTD_human
HgeneDDIT3C0028840Ocular Hypertension1CTD_human
HgeneDDIT3C0041582Ulcer1CTD_human
HgeneDDIT3C0087031Juvenile-Onset Still Disease1CTD_human
HgeneDDIT3C0162351Contact hypersensitivity1CTD_human
HgeneDDIT3C0205734Diabetes, Autoimmune1CTD_human
HgeneDDIT3C0342302Brittle diabetes1CTD_human
HgeneDDIT3C0678222Breast Carcinoma1CTD_human
HgeneDDIT3C1257931Mammary Neoplasms, Human1CTD_human
HgeneDDIT3C1458155Mammary Neoplasms1CTD_human
HgeneDDIT3C2711227Steatohepatitis1CTD_human
HgeneDDIT3C2930617Pulmonary Fibrosis - from Asbestos Exposure1CTD_human
HgeneDDIT3C3495559Juvenile arthritis1CTD_human
HgeneDDIT3C3714758Juvenile psoriatic arthritis1CTD_human
HgeneDDIT3C3837958Diabetes Mellitus, Ketosis-Prone1CTD_human
HgeneDDIT3C4552091Polyarthritis, Juvenile, Rheumatoid Factor Negative1CTD_human
HgeneDDIT3C4554117Diabetes Mellitus, Sudden-Onset1CTD_human
HgeneDDIT3C4704862Polyarthritis, Juvenile, Rheumatoid Factor Positive1CTD_human
HgeneDDIT3C4704874Mammary Carcinoma, Human1CTD_human
TgeneC0006826Malignant Neoplasms1CTD_human
TgeneC0027651Neoplasms1CTD_human
TgeneC0086692Benign Neoplasm1CTD_human
TgeneC0235874Disease Exacerbation1CTD_human