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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:EWSR1-CREB1 (FusionGDB2 ID:HG2130TG1385)

Fusion Gene Summary for EWSR1-CREB1

check button Fusion gene summary
Fusion gene informationFusion gene name: EWSR1-CREB1
Fusion gene ID: hg2130tg1385
HgeneTgene
Gene symbol

EWSR1

CREB1

Gene ID

2130

1385

Gene nameEWS RNA binding protein 1cAMP responsive element binding protein 1
SynonymsEWS|EWS-FLI1|bK984G1.4CREB|CREB-1
Cytomap('EWSR1')('CREB1')

22q12.2

2q33.3

Type of geneprotein-codingprotein-coding
DescriptionRNA-binding protein EWSEWS RNA-binding protein variant 6Ewing sarcoma breakpoint region 1Ewings sarcoma EWS-Fli1 (type 1) oncogenecyclic AMP-responsive element-binding protein 1active transcription factor CREBcAMP-response element-binding protein-1cyclic adenosine 3',5'-monophosphate response element binding proteincyclic adenosine 3',5'-monophosphate response element-binding pr
Modification date2020032920200315
UniProtAcc

Q01844

P16220

Ensembl transtripts involved in fusion geneENST00000331029, ENST00000332050, 
ENST00000333395, ENST00000397938, 
ENST00000406548, ENST00000414183, 
ENST00000332035, 
Fusion gene scores* DoF score44 X 91 X 16=640647 X 8 X 4=224
# samples 987
** MAII scorelog2(98/64064*10)=-6.03058831983342
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(7/224*10)=-1.67807190511264
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: EWSR1 [Title/Abstract] AND CREB1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint
Anticipated loss of major functional domain due to fusion event.EWSR1-CREB1 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
EWSR1-CREB1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
EWSR1-CREB1 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
EWSR1-CREB1 seems lost the major protein functional domain in Tgene partner, which is a transcription factor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneCREB1

GO:0006468

protein phosphorylation

8798441

TgeneCREB1

GO:0010033

response to organic substance

8798441

TgeneCREB1

GO:0010944

negative regulation of transcription by competitive promoter binding

19861239

TgeneCREB1

GO:0045944

positive regulation of transcription by RNA polymerase II

1655749|8798441|19861239



check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerKB3..EWSR1chr22

29678546

+CREB1chr2

208439995

+
ChimerKB3..EWSR1chr22

29678546

+CREB1chr2

208442228

+
ChimerKB4..EWSR1chr22

29678546

+CREB1chr2

208439995

+


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Fusion Gene ORF analysis for EWSR1-CREB1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-3UTRENST00000331029ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-3UTRENST00000332050ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-3UTRENST00000333395ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-3UTRENST00000397938ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-3UTRENST00000406548ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-3UTRENST00000414183ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000331029ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000331029ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000331029ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000331029ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000331029ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000331029ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000332050ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000332050ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000332050ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000332050ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000332050ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000332050ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000333395ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000333395ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000333395ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000333395ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000333395ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000333395ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000397938ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000397938ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000397938ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000397938ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000397938ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000397938ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000406548ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000406548ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000406548ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000406548ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000406548ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000406548ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000414183ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000414183ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000414183ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208439995

+
5CDS-intronENST00000414183ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000414183ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208442228

+
5CDS-intronENST00000414183ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000331029ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000331029ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000331029ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000331029ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000331029ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000331029ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000331029ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000332050ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000332050ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000332050ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000332050ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000332050ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000332050ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000332050ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000333395ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000333395ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000333395ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000333395ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000333395ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000333395ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000333395ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000397938ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000397938ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000397938ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000397938ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000397938ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000397938ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000397938ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000406548ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000406548ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000406548ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000406548ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000406548ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000406548ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000406548ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000414183ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000414183ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000414183ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000414183ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000414183ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208439995

+
Frame-shiftENST00000414183ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208442228

+
Frame-shiftENST00000414183ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208439995

+
intron-3CDSENST00000332035ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208439995

+
intron-3CDSENST00000332035ENST00000353267EWSR1chr22

29678546

+CREB1chr2

208442228

+
intron-3CDSENST00000332035ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208439995

+
intron-3CDSENST00000332035ENST00000430624EWSR1chr22

29678546

+CREB1chr2

208442228

+
intron-3CDSENST00000332035ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208439995

+
intron-3CDSENST00000332035ENST00000432329EWSR1chr22

29678546

+CREB1chr2

208442228

+
intron-3CDSENST00000332035ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208439995

+
intron-3UTRENST00000332035ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208439995

+
intron-intronENST00000332035ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208439995

+
intron-intronENST00000332035ENST00000374397EWSR1chr22

29678546

+CREB1chr2

208442228

+
intron-intronENST00000332035ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208439995

+
intron-intronENST00000332035ENST00000451164EWSR1chr22

29678546

+CREB1chr2

208442228

+
intron-intronENST00000332035ENST00000536726EWSR1chr22

29678546

+CREB1chr2

208442228

+
intron-intronENST00000332035ENST00000539789EWSR1chr22

29678546

+CREB1chr2

208442228

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for EWSR1-CREB1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


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Fusion Protein Features for EWSR1-CREB1


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:/:)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
EWSR1

Q01844

CREB1

P16220

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for EWSR1-CREB1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for EWSR1-CREB1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for EWSR1-CREB1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status
TgeneCREB1P16220DB00131Adenosine phosphateActivatorSmall moleculeApproved|Investigational|Nutraceutical
TgeneCREB1P16220DB01183NaloxoneOther/unknownSmall moleculeApproved|Vet_approved

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Related Diseases for EWSR1-CREB1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneEWSR1C0553580Ewings sarcoma3CTD_human;ORPHANET
HgeneEWSR1C0002736Amyotrophic Lateral Sclerosis1GENOMICS_ENGLAND
HgeneEWSR1C0033578Prostatic Neoplasms1CTD_human
HgeneEWSR1C0206651Clear Cell Sarcoma of Soft Tissue1ORPHANET
HgeneEWSR1C0206663Neuroectodermal Tumor, Primitive1CTD_human
HgeneEWSR1C0279980Extra-osseous Ewing's sarcoma1ORPHANET
HgeneEWSR1C0334584Spongioblastoma1CTD_human
HgeneEWSR1C0334596Medulloepithelioma1CTD_human
HgeneEWSR1C0376358Malignant neoplasm of prostate1CTD_human
HgeneEWSR1C0700367Ependymoblastoma1CTD_human
HgeneEWSR1C0751675Cerebral Primitive Neuroectodermal Tumor1CTD_human
HgeneEWSR1C1275278Extraskeletal Myxoid Chondrosarcoma1ORPHANET
TgeneC0011570Mental Depression5PSYGENET
TgeneC0011581Depressive disorder5PSYGENET
TgeneC0041696Unipolar Depression5PSYGENET
TgeneC0525045Mood Disorders5PSYGENET
TgeneC1269683Major Depressive Disorder5PSYGENET
TgeneC0600427Cocaine Dependence4CTD_human;PSYGENET
TgeneC0001973Alcoholic Intoxication, Chronic3PSYGENET
TgeneC0005586Bipolar Disorder3PSYGENET
TgeneC0009171Cocaine Abuse3CTD_human
TgeneC0036341Schizophrenia3PSYGENET
TgeneC0236736Cocaine-Related Disorders3CTD_human
TgeneC0026552Morphine Dependence1CTD_human
TgeneC0027051Myocardial Infarction1CTD_human
TgeneC0027627Neoplasm Metastasis1CTD_human
TgeneC0038587Substance Withdrawal Syndrome1CTD_human
TgeneC0086189Drug Withdrawal Symptoms1CTD_human
TgeneC0087169Withdrawal Symptoms1CTD_human
TgeneC0151779Cutaneous Melanoma1CTD_human
TgeneC0206651Clear Cell Sarcoma of Soft Tissue1ORPHANET
TgeneC0600272Morphine Abuse1CTD_human