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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:MAPK9-STK4 (FusionGDB2 ID:HG5601TG6789)

Fusion Gene Summary for MAPK9-STK4

check button Fusion gene summary
Fusion gene informationFusion gene name: MAPK9-STK4
Fusion gene ID: hg5601tg6789
HgeneTgene
Gene symbol

MAPK9

STK4

Gene ID

5601

6789

Gene namemitogen-activated protein kinase 9serine/threonine kinase 4
SynonymsJNK-55|JNK2|JNK2A|JNK2ALPHA|JNK2B|JNK2BETA|PRKM9|SAPK|SAPK1a|p54a|p54aSAPKKRS2|MST1|YSK3
Cytomap('MAPK9')('STK4')

5q35.3

20q13.12

Type of geneprotein-codingprotein-coding
Descriptionmitogen-activated protein kinase 9Jun kinaseMAP kinase 9MAPK 9c-Jun N-terminal kinase 2c-Jun kinase 2stress-activated protein kinase 1astress-activated protein kinase JNK2serine/threonine-protein kinase 4STE20-like kinase MST1kinase responsive to stress 2mammalian STE20-like protein kinase 1mammalian sterile 20-like 1serine/threonine-protein kinase Krs-2
Modification date2020032720200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000343111, ENST00000347470, 
ENST00000393360, ENST00000425491, 
ENST00000452135, ENST00000455781, 
ENST00000397072, ENST00000524170, 
ENST00000539014, 
Fusion gene scores* DoF score9 X 10 X 6=54013 X 13 X 7=1183
# samples 1016
** MAII scorelog2(10/540*10)=-2.43295940727611
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(16/1183*10)=-2.88630626345243
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: MAPK9 [Title/Abstract] AND STK4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointMAPK9(179718847)-STK4(43653613), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneMAPK9

GO:0007254

JNK cascade

8654373

HgeneMAPK9

GO:0018105

peptidyl-serine phosphorylation

21856198

TgeneSTK4

GO:0000902

cell morphogenesis

11805089

TgeneSTK4

GO:0001934

positive regulation of protein phosphorylation

19962960

TgeneSTK4

GO:0006468

protein phosphorylation

8566796|11805089

TgeneSTK4

GO:0006915

apoptotic process

21212262

TgeneSTK4

GO:0018105

peptidyl-serine phosphorylation

16751106

TgeneSTK4

GO:0032092

positive regulation of protein binding

22292086

TgeneSTK4

GO:0033138

positive regulation of peptidyl-serine phosphorylation

19962960

TgeneSTK4

GO:0035329

hippo signaling

20412773

TgeneSTK4

GO:0035556

intracellular signal transduction

8566796

TgeneSTK4

GO:0043065

positive regulation of apoptotic process

15109305

TgeneSTK4

GO:0046777

protein autophosphorylation

11805089

TgeneSTK4

GO:0050821

protein stabilization

19962960



check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4COADTCGA-A6-2674MAPK9chr5

179718847

-STK4chr20

43653613

+


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Fusion Gene ORF analysis for MAPK9-STK4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5UTR-3CDSENST00000343111ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000343111ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000343111ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000343111ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000347470ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000347470ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000347470ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000347470ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000393360ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000393360ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000393360ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000393360ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000425491ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000425491ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000425491ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000425491ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000452135ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000452135ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000452135ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000452135ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000455781ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000455781ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000455781ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-3CDSENST00000455781ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-intronENST00000343111ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-intronENST00000347470ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-intronENST00000393360ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-intronENST00000425491ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-intronENST00000452135ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+
5UTR-intronENST00000455781ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000397072ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000397072ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000397072ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000397072ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000524170ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000524170ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000524170ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000524170ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000539014ENST00000372801MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000539014ENST00000372806MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000539014ENST00000396731MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-3CDSENST00000539014ENST00000499879MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-intronENST00000397072ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-intronENST00000524170ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+
intron-intronENST00000539014ENST00000487587MAPK9chr5

179718847

-STK4chr20

43653613

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for MAPK9-STK4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for MAPK9-STK4


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:179718847/:43653613)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for MAPK9-STK4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for MAPK9-STK4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for MAPK9-STK4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for MAPK9-STK4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneMAPK9C0035126Reperfusion Injury2CTD_human
HgeneMAPK9C0005398Cholestasis, Extrahepatic1CTD_human
HgeneMAPK9C0020429Hyperalgesia1CTD_human
HgeneMAPK9C0022116Ischemia1CTD_human
HgeneMAPK9C0027627Neoplasm Metastasis1CTD_human
HgeneMAPK9C0032927Precancerous Conditions1CTD_human
HgeneMAPK9C0040997Trigeminal Neuralgia1CTD_human
HgeneMAPK9C0282313Condition, Preneoplastic1CTD_human
HgeneMAPK9C0393786Trigeminal Neuralgia, Idiopathic1CTD_human
HgeneMAPK9C0393787Secondary Trigeminal Neuralgia1CTD_human
HgeneMAPK9C0458247Allodynia1CTD_human
HgeneMAPK9C0751211Hyperalgesia, Primary1CTD_human
HgeneMAPK9C0751212Hyperalgesia, Secondary1CTD_human
HgeneMAPK9C0751213Tactile Allodynia1CTD_human
HgeneMAPK9C0751214Hyperalgesia, Thermal1CTD_human
HgeneMAPK9C2936719Mechanical Allodynia1CTD_human
TgeneC3553943T-CELL IMMUNODEFICIENCY, RECURRENT INFECTIONS, AND AUTOIMMUNITY WITH OR WITHOUT CARDIAC MALFORMATIONS2CTD_human;GENOMICS_ENGLAND;ORPHANET
TgeneC4016913T-CELL IMMUNODEFICIENCY, RECURRENT INFECTIONS, AUTOIMMUNITY, AND CARDIAC MALFORMATIONS2GENOMICS_ENGLAND
TgeneC0005586Bipolar Disorder1CTD_human
TgeneC0005587Depression, Bipolar1CTD_human
TgeneC0024713Manic Disorder1CTD_human
TgeneC0338831Manic1CTD_human
TgeneC0494261Combined immunodeficiency1GENOMICS_ENGLAND