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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:SSB-CASP8 (FusionGDB2 ID:HG6741TG841)

Fusion Gene Summary for SSB-CASP8

check button Fusion gene summary
Fusion gene informationFusion gene name: SSB-CASP8
Fusion gene ID: hg6741tg841
HgeneTgene
Gene symbol

SSB

CASP8

Gene ID

6741

841

Gene namesmall RNA binding exonuclease protection factor Lacaspase 8
SynonymsLARP3|La|La/SSBALPS2B|CAP4|Casp-8|FLICE|MACH|MCH5
Cytomap('SSB')('CASP8')

2q31.1

2q33.1

Type of geneprotein-codingprotein-coding
Descriptionlupus La proteinLa ribonucleoprotein domain family, member 3SS-BSS-B/La proteinSjogren syndrome antigen Bautoantigen Lala autoantigenlupus La antigensjoegren syndrome type B antigencaspase-8FADD-homologous ICE/CED-3-like proteaseFADD-like ICEICE-like apoptotic protease 5MACH-alpha-1/2/3 proteinMACH-beta-1/2/3/4 proteinMORT1-associated ced-3 homologapoptotic cysteine proteaseapoptotic protease Mch-5caspase 8, apoptosis-relat
Modification date2020031320200322
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000260956, ENST00000409333, 
Fusion gene scores* DoF score4 X 4 X 2=324 X 4 X 3=48
# samples 45
** MAII scorelog2(4/32*10)=0.321928094887362
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0		log2(5/48*10)=0.0588936890535686
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: SSB [Title/Abstract] AND CASP8 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointSSB(170655486)-CASP8(202131184), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneSSB

GO:0075522

IRES-dependent viral translational initiation

12384597

HgeneSSB

GO:1903608

protein localization to cytoplasmic stress granule

24965446

TgeneCASP8

GO:0006508

proteolysis

12888622

TgeneCASP8

GO:0036462

TRAIL-activated apoptotic signaling pathway

21785459

TgeneCASP8

GO:0045862

positive regulation of proteolysis

18387192

TgeneCASP8

GO:0097191

extrinsic apoptotic signaling pathway

21785459

TgeneCASP8

GO:0097202

activation of cysteine-type endopeptidase activity

18387192



check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUADTCGA-44-2665-01ASSBchr2

170655486

+CASP8chr2

202131184

+


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Fusion Gene ORF analysis for SSB-CASP8

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5UTR-5UTRENST00000260956ENST00000264274SSBchr2

170655486

+CASP8chr2

202131184

+
5UTR-5UTRENST00000260956ENST00000264275SSBchr2

170655486

+CASP8chr2

202131184

+
5UTR-5UTRENST00000260956ENST00000323492SSBchr2

170655486

+CASP8chr2

202131184

+
5UTR-5UTRENST00000260956ENST00000392258SSBchr2

170655486

+CASP8chr2

202131184

+
5UTR-5UTRENST00000260956ENST00000392259SSBchr2

170655486

+CASP8chr2

202131184

+
5UTR-5UTRENST00000260956ENST00000392266SSBchr2

170655486

+CASP8chr2

202131184

+
5UTR-intronENST00000260956ENST00000358485SSBchr2

170655486

+CASP8chr2

202131184

+
5UTR-intronENST00000260956ENST00000432109SSBchr2

170655486

+CASP8chr2

202131184

+
5UTR-intronENST00000260956ENST00000490682SSBchr2

170655486

+CASP8chr2

202131184

+
intron-5UTRENST00000409333ENST00000264274SSBchr2

170655486

+CASP8chr2

202131184

+
intron-5UTRENST00000409333ENST00000264275SSBchr2

170655486

+CASP8chr2

202131184

+
intron-5UTRENST00000409333ENST00000323492SSBchr2

170655486

+CASP8chr2

202131184

+
intron-5UTRENST00000409333ENST00000392258SSBchr2

170655486

+CASP8chr2

202131184

+
intron-5UTRENST00000409333ENST00000392259SSBchr2

170655486

+CASP8chr2

202131184

+
intron-5UTRENST00000409333ENST00000392266SSBchr2

170655486

+CASP8chr2

202131184

+
intron-intronENST00000409333ENST00000358485SSBchr2

170655486

+CASP8chr2

202131184

+
intron-intronENST00000409333ENST00000432109SSBchr2

170655486

+CASP8chr2

202131184

+
intron-intronENST00000409333ENST00000490682SSBchr2

170655486

+CASP8chr2

202131184

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for SSB-CASP8


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
SSBchr2170655486+CASP8chr2202131183+0.0001354550.9998646
SSBchr2170655486+CASP8chr2202131183+0.0001354550.9998646


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for SSB-CASP8


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:170655486/:202131184)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for SSB-CASP8


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for SSB-CASP8


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for SSB-CASP8


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for SSB-CASP8


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC0006142Malignant neoplasm of breast4CGI;CTD_human
TgeneC0678222Breast Carcinoma4CGI;CTD_human
TgeneC1257931Mammary Neoplasms, Human4CTD_human
TgeneC1458155Mammary Neoplasms4CTD_human
TgeneC4704874Mammary Carcinoma, Human4CTD_human
TgeneC1846545Autoimmune Lymphoproliferative Syndrome Type 2B3CTD_human;ORPHANET;UNIPROT
TgeneC0014859Esophageal Neoplasms2CTD_human
TgeneC0025202melanoma2CTD_human
TgeneC0546837Malignant neoplasm of esophagus2CTD_human
TgeneC0001418Adenocarcinoma1CTD_human
TgeneC0005586Bipolar Disorder1PSYGENET
TgeneC0007114Malignant neoplasm of skin1CTD_human
TgeneC0007131Non-Small Cell Lung Carcinoma1CTD_human
TgeneC0007873Uterine Cervical Neoplasm1CTD_human
TgeneC0009402Colorectal Carcinoma1CTD_human
TgeneC0009404Colorectal Neoplasms1CTD_human
TgeneC0011616Contact Dermatitis1CTD_human
TgeneC0011860Diabetes Mellitus, Non-Insulin-Dependent1CTD_human
TgeneC0013604Edema1CTD_human
TgeneC0023452Childhood Acute Lymphoblastic Leukemia1CTD_human
TgeneC0023453L2 Acute Lymphoblastic Leukemia1CTD_human
TgeneC0024121Lung Neoplasms1CTD_human
TgeneC0024623Malignant neoplasm of stomach1CTD_human
TgeneC0027055Myocardial Reperfusion Injury1CTD_human
TgeneC0035126Reperfusion Injury1CTD_human
TgeneC0037286Skin Neoplasms1CTD_human
TgeneC0038220Status Epilepticus1CTD_human
TgeneC0038356Stomach Neoplasms1CTD_human
TgeneC0151603Anasarca1CTD_human
TgeneC0162351Contact hypersensitivity1CTD_human
TgeneC0162820Dermatitis, Allergic Contact1CTD_human
TgeneC0205641Adenocarcinoma, Basal Cell1CTD_human
TgeneC0205642Adenocarcinoma, Oxyphilic1CTD_human
TgeneC0205643Carcinoma, Cribriform1CTD_human
TgeneC0205644Carcinoma, Granular Cell1CTD_human
TgeneC0205645Adenocarcinoma, Tubular1CTD_human
TgeneC0242379Malignant neoplasm of lung1CTD_human
TgeneC0270823Petit mal status1CTD_human
TgeneC0279607Adult Hepatocellular Carcinoma1ORPHANET
TgeneC0311335Grand Mal Status Epilepticus1CTD_human
TgeneC0343641Human papilloma virus infection1CTD_human
TgeneC0393734Complex Partial Status Epilepticus1CTD_human
TgeneC0751522Status Epilepticus, Subclinical1CTD_human
TgeneC0751523Non-Convulsive Status Epilepticus1CTD_human
TgeneC0751524Simple Partial Status Epilepticus1CTD_human
TgeneC1168401Squamous cell carcinoma of the head and neck1CTD_human
TgeneC1708349Hereditary Diffuse Gastric Cancer1CTD_human
TgeneC1961102Precursor Cell Lymphoblastic Leukemia Lymphoma1CTD_human
TgeneC2931456Prostate cancer, familial1CTD_human
TgeneC2937358Cerebral Hemorrhage1CTD_human
TgeneC4048328cervical cancer1CTD_human
TgeneC4722327PROSTATE CANCER, HEREDITARY, 11CTD_human