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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:APC2-MPHOSPH8 (FusionGDB2 ID:HG10297TG54737)

Fusion Gene Summary for APC2-MPHOSPH8

check button Fusion gene summary
Fusion gene informationFusion gene name: APC2-MPHOSPH8
Fusion gene ID: hg10297tg54737
HgeneTgene
Gene symbol

APC2

MPHOSPH8

Gene ID

10297

54737

Gene nameAPC regulator of WNT signaling pathway 2M-phase phosphoprotein 8
SynonymsAPCLHSMPP8|TWA3|mpp8
Cytomap('APC2')('MPHOSPH8')

19p13.3

13q12.11

Type of geneprotein-codingprotein-coding
Descriptionadenomatous polyposis coli protein 2APC2, WNT signaling pathway regulatoradenomatosis polyposis coli 2adenomatous polyposis coli likeM-phase phosphoprotein 8M-phase phosphoprotein, mppM-phase phosphoprotein, mpp8two hybrid-associated protein 3 with RanBPM
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000233607, ENST00000238483, 
ENST00000535453, 
Fusion gene scores* DoF score2 X 2 X 2=825 X 17 X 10=4250
# samples 230
** MAII scorelog2(2/8*10)=1.32192809488736log2(30/4250*10)=-3.82442843541655
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: APC2 [Title/Abstract] AND MPHOSPH8 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointAPC2(1462176)-MPHOSPH8(20245346), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneAPC2

GO:0090090

negative regulation of canonical Wnt signaling pathway

9823329|10021369

TgeneMPHOSPH8

GO:0045814

negative regulation of gene expression, epigenetic

26022416|28581500|29211708

TgeneMPHOSPH8

GO:0090309

positive regulation of methylation-dependent chromatin silencing

28581500|29211708



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4GBMTCGA-14-1402-02AAPC2chr19

1462176

+MPHOSPH8chr13

20245346

+


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Fusion Gene ORF analysis for APC2-MPHOSPH8

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-3UTRENST00000233607ENST00000496525APC2chr19

1462176

+MPHOSPH8chr13

20245346

+
5CDS-3UTRENST00000238483ENST00000496525APC2chr19

1462176

+MPHOSPH8chr13

20245346

+
5CDS-3UTRENST00000535453ENST00000496525APC2chr19

1462176

+MPHOSPH8chr13

20245346

+
5CDS-intronENST00000233607ENST00000361479APC2chr19

1462176

+MPHOSPH8chr13

20245346

+
5CDS-intronENST00000233607ENST00000414242APC2chr19

1462176

+MPHOSPH8chr13

20245346

+
5CDS-intronENST00000238483ENST00000361479APC2chr19

1462176

+MPHOSPH8chr13

20245346

+
5CDS-intronENST00000238483ENST00000414242APC2chr19

1462176

+MPHOSPH8chr13

20245346

+
5CDS-intronENST00000535453ENST00000361479APC2chr19

1462176

+MPHOSPH8chr13

20245346

+
5CDS-intronENST00000535453ENST00000414242APC2chr19

1462176

+MPHOSPH8chr13

20245346

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for APC2-MPHOSPH8


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
APC2chr191462176+MPHOSPH8chr1320245345+7.46E-060.9999925
APC2chr191462176+MPHOSPH8chr1320245345+7.46E-060.9999925


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for APC2-MPHOSPH8


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:1462176/:20245346)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for APC2-MPHOSPH8


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for APC2-MPHOSPH8


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for APC2-MPHOSPH8


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for APC2-MPHOSPH8


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneAPC2C0009402Colorectal Carcinoma3CTD_human
HgeneAPC2C0009404Colorectal Neoplasms3CTD_human
HgeneAPC2C0006142Malignant neoplasm of breast1CTD_human;UNIPROT
HgeneAPC2C0021390Inflammatory Bowel Diseases1CTD_human
HgeneAPC2C0036572Seizures1GENOMICS_ENGLAND
HgeneAPC2C0042063Urogenital Abnormalities1GENOMICS_ENGLAND
HgeneAPC2C0175695Sotos' syndrome1ORPHANET
HgeneAPC2C0243050Cardiovascular Abnormalities1GENOMICS_ENGLAND
HgeneAPC2C0557874Global developmental delay1GENOMICS_ENGLAND
HgeneAPC2C0678222Breast Carcinoma1CTD_human
HgeneAPC2C1257931Mammary Neoplasms, Human1CTD_human
HgeneAPC2C1458155Mammary Neoplasms1CTD_human
HgeneAPC2C3714756Intellectual Disability1GENOMICS_ENGLAND
HgeneAPC2C4021765Morphological abnormality of the central nervous system1GENOMICS_ENGLAND
HgeneAPC2C4021790Abnormality of the skeletal system1GENOMICS_ENGLAND
HgeneAPC2C4022810Abnormality of nervous system morphology1GENOMICS_ENGLAND
HgeneAPC2C4025860Hearing abnormality1GENOMICS_ENGLAND
HgeneAPC2C4316870Abnormality of the eye1GENOMICS_ENGLAND
HgeneAPC2C4704874Mammary Carcinoma, Human1CTD_human