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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ARIH2-RHOA (FusionGDB2 ID:HG10425TG387)

Fusion Gene Summary for ARIH2-RHOA

check button Fusion gene summary
Fusion gene informationFusion gene name: ARIH2-RHOA
Fusion gene ID: hg10425tg387
HgeneTgene
Gene symbol

ARIH2

RHOA

Gene ID

10425

387

Gene nameariadne RBR E3 ubiquitin protein ligase 2ras homolog family member A
SynonymsARI2|TRIAD1ARH12|ARHA|EDFAOB|RHO12|RHOH12
Cytomap('ARIH2')('RHOA')

3p21.31

3p21.31

Type of geneprotein-codingprotein-coding
DescriptionE3 ubiquitin-protein ligase ARIH2RING-type E3 ubiquitin transferase ARIH2all-trans retinoic acid inducible RING fingerariadne homolog 2protein ariadne-2 homologtransforming protein RhoAAplysia ras-related homolog 12epididymis secretory sperm binding proteinoncogene RHO H12small GTP binding protein RhoA
Modification date2020031320200329
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000356401, ENST00000449376, 
ENST00000490095, 
Fusion gene scores* DoF score10 X 9 X 7=63025 X 14 X 7=2450
# samples 1231
** MAII scorelog2(12/630*10)=-2.39231742277876
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(31/2450*10)=-2.9824416286157
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ARIH2 [Title/Abstract] AND RHOA [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointARIH2(48960244)-RHOA(49413024), # samples:4
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneARIH2

GO:0000209

protein polyubiquitination

16118314|19340006

HgeneARIH2

GO:0006511

ubiquitin-dependent protein catabolic process

16118314

HgeneARIH2

GO:0016567

protein ubiquitination

24076655

HgeneARIH2

GO:0048588

developmental cell growth

19340006

HgeneARIH2

GO:0070534

protein K63-linked ubiquitination

19340006

HgeneARIH2

GO:0070936

protein K48-linked ubiquitination

16118314|19340006

HgeneARIH2

GO:0071425

hematopoietic stem cell proliferation

16118314|19340006

TgeneRHOA

GO:0007266

Rho protein signal transduction

26529257

TgeneRHOA

GO:0016477

cell migration

26529257

TgeneRHOA

GO:0032956

regulation of actin cytoskeleton organization

25911094

TgeneRHOA

GO:0035385

Roundabout signaling pathway

26529257

TgeneRHOA

GO:0036089

cleavage furrow formation

16103226

TgeneRHOA

GO:0051496

positive regulation of stress fiber assembly

15467718

TgeneRHOA

GO:0060193

positive regulation of lipase activity

19887681

TgeneRHOA

GO:0071222

cellular response to lipopolysaccharide

19734146

TgeneRHOA

GO:0071902

positive regulation of protein serine/threonine kinase activity

8617235



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BLCATCGA-FD-A3B8-01AARIH2chr3

48960244

-RHOAchr3

49413024

-
ChimerDB4BLCATCGA-FD-A3B8-01AARIH2chr3

48960244

+RHOAchr3

49413024

-
ChimerDB4BLCATCGA-FD-A3B8ARIH2chr3

48960244

+RHOAchr3

49413024

-


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Fusion Gene ORF analysis for ARIH2-RHOA

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5UTR-5UTRENST00000356401ENST00000418115ARIH2chr3

48960244

+RHOAchr3

49413024

-
5UTR-5UTRENST00000356401ENST00000422781ARIH2chr3

48960244

+RHOAchr3

49413024

-
5UTR-5UTRENST00000356401ENST00000454011ARIH2chr3

48960244

+RHOAchr3

49413024

-
5UTR-5UTRENST00000449376ENST00000418115ARIH2chr3

48960244

+RHOAchr3

49413024

-
5UTR-5UTRENST00000449376ENST00000422781ARIH2chr3

48960244

+RHOAchr3

49413024

-
5UTR-5UTRENST00000449376ENST00000454011ARIH2chr3

48960244

+RHOAchr3

49413024

-
5UTR-intronENST00000356401ENST00000265538ARIH2chr3

48960244

+RHOAchr3

49413024

-
5UTR-intronENST00000449376ENST00000265538ARIH2chr3

48960244

+RHOAchr3

49413024

-
intron-5UTRENST00000490095ENST00000418115ARIH2chr3

48960244

+RHOAchr3

49413024

-
intron-5UTRENST00000490095ENST00000422781ARIH2chr3

48960244

+RHOAchr3

49413024

-
intron-5UTRENST00000490095ENST00000454011ARIH2chr3

48960244

+RHOAchr3

49413024

-
intron-intronENST00000490095ENST00000265538ARIH2chr3

48960244

+RHOAchr3

49413024

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ARIH2-RHOA


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


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Fusion Protein Features for ARIH2-RHOA


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:48960244/:49413024)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ARIH2-RHOA


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ARIH2-RHOA


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ARIH2-RHOA


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ARIH2-RHOA


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC0024623Malignant neoplasm of stomach3CTD_human
TgeneC0038356Stomach Neoplasms3CTD_human
TgeneC1708349Hereditary Diffuse Gastric Cancer3CTD_human
TgeneC0020981Angioimmunoblastic Lymphadenopathy2CTD_human
TgeneC0079774Peripheral T-Cell Lymphoma2CTD_human
TgeneC0000771Abnormalities, Drug-Induced1CTD_human
TgeneC0002170Alopecia1CTD_human
TgeneC0005684Malignant neoplasm of urinary bladder1CTD_human
TgeneC0005695Bladder Neoplasm1CTD_human
TgeneC0007097Carcinoma1CTD_human
TgeneC0009171Cocaine Abuse1CTD_human
TgeneC0013575Ectodermal Dysplasia1CTD_human
TgeneC0027626Neoplasm Invasiveness1CTD_human
TgeneC0033687Proteinuria1CTD_human
TgeneC0038587Substance Withdrawal Syndrome1CTD_human
TgeneC0040435Tooth Diseases1CTD_human
TgeneC0079772T-Cell Lymphoma1CTD_human
TgeneC0079773Lymphoma, T-Cell, Cutaneous1CTD_human
TgeneC0086189Drug Withdrawal Symptoms1CTD_human
TgeneC0086873Pseudopelade1CTD_human
TgeneC0087169Withdrawal Symptoms1CTD_human
TgeneC0162311Androgenetic Alopecia1CTD_human
TgeneC0162361Hidrotic Ectodermal Dysplasia1CTD_human
TgeneC0162835Hypopigmentation disorder1CTD_human
TgeneC0205696Anaplastic carcinoma1CTD_human
TgeneC0205697Carcinoma, Spindle-Cell1CTD_human
TgeneC0205698Undifferentiated carcinoma1CTD_human
TgeneC0205699Carcinomatosis1CTD_human
TgeneC0235833Congenital diaphragmatic hernia1CTD_human
TgeneC0236736Cocaine-Related Disorders1CTD_human
TgeneC0263477Female pattern alopecia (disorder)1CTD_human
TgeneC0265316Neurocutaneous Syndromes1CTD_human
TgeneC0265699Congenital hernia of foramen of Morgagni1CTD_human
TgeneC0265700Congenital hernia of foramen of Bochdalek1CTD_human
TgeneC0270612Leukoencephalopathy1CTD_human
TgeneC0282160Aplasia Cutis Congenita1CTD_human
TgeneC0376407Granulomatous Slack Skin1CTD_human
TgeneC0376634Craniofacial Abnormalities1CTD_human
TgeneC0600427Cocaine Dependence1CTD_human
TgeneC1706004Anhydrotic Ectodermal Dysplasias1CTD_human
TgeneC1744559Congenital ectodermal dysplasia of face1CTD_human
TgeneC1858991Childhood Ataxia with Central Nervous System Hypomyelinization1CTD_human
TgeneC4083212Alopecia, Male Pattern1CTD_human