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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ANP32B-GTPBP4 (FusionGDB2 ID:HG10541TG23560)

Fusion Gene Summary for ANP32B-GTPBP4

check button Fusion gene summary
Fusion gene informationFusion gene name: ANP32B-GTPBP4
Fusion gene ID: hg10541tg23560
HgeneTgene
Gene symbol

ANP32B

GTPBP4

Gene ID

10541

23560

Gene nameacidic nuclear phosphoprotein 32 family member BGTP binding protein 4
SynonymsAPRIL|PHAPI2|SSP29CRFG|NGB|NOG1
Cytomap('ANP32B')('GTPBP4')

9q22.33

10p15.3

Type of geneprotein-codingprotein-coding
Descriptionacidic leucine-rich nuclear phosphoprotein 32 family member Bacidic (leucine-rich) nuclear phosphoprotein 32 family, member Bacidic protein rich in leucinesputative HLA-DR-associated protein I-2silver-stainable protein SSP29nucleolar GTP-binding protein 1G protein-binding protein CRFGGTP-binding protein NGBchronic renal failure gene protein
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000339399, ENST00000473205, 
Fusion gene scores* DoF score12 X 13 X 4=6245 X 5 X 2=50
# samples 135
** MAII scorelog2(13/624*10)=-2.26303440583379
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(5/50*10)=0
Context

PubMed: ANP32B [Title/Abstract] AND GTPBP4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointANP32B(100773593)-GTPBP4(1063014), # samples:1
Anticipated loss of major functional domain due to fusion event.ANP32B-GTPBP4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ANP32B-GTPBP4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneANP32B

GO:0006334

nucleosome assembly

20538007

HgeneANP32B

GO:0045596

negative regulation of cell differentiation

22705300

TgeneGTPBP4

GO:0000079

regulation of cyclin-dependent protein serine/threonine kinase activity

17210637

TgeneGTPBP4

GO:0022408

negative regulation of cell-cell adhesion

17210637

TgeneGTPBP4

GO:0030336

negative regulation of cell migration

17210637

TgeneGTPBP4

GO:0031397

negative regulation of protein ubiquitination

17210637

TgeneGTPBP4

GO:0050821

protein stabilization

17210637


check buttonFusion gene breakpoints across ANP32B (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure
check buttonFusion gene breakpoints across GTPBP4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand


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Fusion Gene ORF analysis for ANP32B-GTPBP4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000339399ENST00000360803ANP32Bchr9100773593+GTPBP4chr101063014+0.0011404930.9988595

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Fusion Genomic Features for ANP32B-GTPBP4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

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Fusion Protein Features for ANP32B-GTPBP4


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr9:100773593/chr10:1063014)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneANP32Bchr9:100773593chr10:1063014ENST00000339399+17146_2510252.0Compositional biasNote=Asp/Glu-rich (highly acidic)
HgeneANP32Bchr9:100773593chr10:1063014ENST00000339399+17123_1610252.0DomainNote=LRRCT
HgeneANP32Bchr9:100773593chr10:1063014ENST00000339399+17239_2420252.0MotifNuclear localization signal
HgeneANP32Bchr9:100773593chr10:1063014ENST00000339399+1716_400252.0RepeatLRR 1
HgeneANP32Bchr9:100773593chr10:1063014ENST00000339399+1743_640252.0RepeatLRR 2
HgeneANP32Bchr9:100773593chr10:1063014ENST00000339399+1765_840252.0RepeatLRR 3
HgeneANP32Bchr9:100773593chr10:1063014ENST00000339399+1789_1100252.0RepeatLRR 4
TgeneGTPBP4chr9:100773593chr10:1063014ENST000003608031517169_340584635.0DomainOBG-type G
TgeneGTPBP4chr9:100773593chr10:1063014ENST000003608031517175_182584635.0Nucleotide bindingGTP
TgeneGTPBP4chr9:100773593chr10:1063014ENST000003608031517221_225584635.0Nucleotide bindingGTP
TgeneGTPBP4chr9:100773593chr10:1063014ENST000003608031517289_292584635.0Nucleotide bindingGTP


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Fusion Gene Sequence for ANP32B-GTPBP4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>4965_4965_1_ANP32B-GTPBP4_ANP32B_chr9_100773593_ENST00000339399_GTPBP4_chr10_1063014_ENST00000360803_length(transcript)=3652nt_BP=790nt
CTCCGCTCCCGTGAGTAACTTGGCTCCGGGGGCTCCGCTCGCCTGCCCGCACGCCGCCCGCCACCCAGGACCGCGCCGCCGGCCTCCGCC
GCTAGCAAACCCTTCCGACGGCCCTCGCTGCGCAAGCCGGGACGCCTCTCCCCCCTCCGCCCCCGCCGCGGAAAGTTAAGTTTGAAGAGG
GGGGAAGAGGGGAACATGGACATGAAGAGGAGGATCCACCTGGAGCTGAGGAACCGGACCCCGGCAGCTGTTCGAGAACTTGTCTTGGAC
AATTGCAAATCAAATGATGGAAAAATTGAGGGCTTAACAGCTGAATTTGTGAACTTAGAGTTCCTCAGTTTAATAAATGTAGGCTTGATC
TCAGTTTCAAATCTCCCCAAGCTGCCTAAATTGAAAAAGCTTGAACTCAGTGAAAATAGAATCTTTGGAGGTCTGGACATGTTAGCTGAA
AAACTTCCAAATCTCACACATCTAAACTTAAGTGGAAATAAACTGAAAGATATCAGCACCTTGGAACCTTTGAAAAAGTTAGAATGTCTG
AAAAGCCTGGACCTCTTTAACTGTGAGGTTACCAACCTGAATGACTACCGAGAGAGTGTCTTCAAGCTCCTGCCCCAGCTTACCTACTTG
GATGGCTATGACCGAGAGGACCAGGAAGCACCTGACTCAGATGCCGAGGTGGATGGTGTGGATGAAGAGGAGGAGGACGAAGGAAGAGGA
GTTTGATGAAGAAGATGATGAAGATGAAGATGTAGAAGGGGATGAGGACGACGATGAAGTCAGTGAGGAGATGGTGAAGAAAGCCAAGAC
TATGATGAAGAATGCTCAGAAGAAGATGAATCGGTTGGGGAAGAAAGGGGAGGCGGATAGACACGTGTTTGATATGAAGCCCAAGCACTT
GCTGTCTGGGAAGAGGAAAGCTGGTAAAAAGGACAGGAGATAGTATCCGTTTGGTTGGCGTGGCTTCGCTAGAGTGTTGCTGTTTATTTC
CTGGTTTGGCACAGTATGGTTTCATGAAATTGGAGCTCTGTATAAACTGAAAAAGACAAAATAAGTAAAGCACTTGTTGCTTTGCTGAAA
ACTATGGTTAACCCTATATAGGTGTGGGAAATTTTTGTCACTGCATAATATTACAAATATTTTGAGTAGACAGTGTTTCCACATTTAATG
GAGTATCAGTTGCTTCAGATTTTCAGAACTGGGAAGATTTACTGGTTTAACTAGGTTGTTTTTGATGGAGAAAAACCTTATTTTCTTTTG
TAAGAGCTGGGAGCAAACACGTTTATGAGTGTGTCGGAATCCCGTGCTTAAAATACGCTCTTAAATTATTTTCTAGTCTTATTTTACAAT
GTCTCATTGTAGTCTGTCTTCAACTATTTTATCCAAAATAAACCTCCAGAAGAAAGTAGTTTTCATTTACTTAGCTCATGTTTTGGTTTA
GTTATAGTCGCTATGGATTTGGCCAAATAAAAAGGCAAACAACATGACATTTCTATTTTGTTTTAAAGCAAAAATACTTTTGGGGGAGTA
AAATGTTGCTGGAGGCATTAGGCAATTAAATATGGGCATTCTCCTATTTCTGTGGCCCGTCCCTGAAGTTACATGTTCAGTAAAACACAA
AAACACCGCCAATGCCACCTCAAAAGCACCTGCTGGGGAGCGTTGGGGTGAAGAGCTGTGGGGTCGGAGGGACCTGCCCAGGGGTCCGTG
TGCTCCTGGAGCTCCAAAGACACACAGGGGTCCAAGAGCCACCACGGATAAGTTATCAAGTCACACAATCAACATTATAGCTGAGGAAAG
GACTTTAGTTAATCACACTTCTGGATTACATGTGGCAATTTTAAAAATTTACCAGGGCTGGGCTGGGCGCAGGGGCTCAAGCCTGTAGTT
CCAGCACTTTGGGAGGCCGAGGTGGGCAGATCAGGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAA
AAATACAAAAATTAGCCATGCGTGGTGGTGGGCATCTGTAATCCTAGCTGCTCGGGAGGCTAAGGCAGGAGAATTGCTTGAGCCCAGAGG
CGGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAGAGAGAGACTCCGTGTGAAAAAAAAAAAAAAAATACCA
GGTCTGGTTTTTGAAATTCTTTCTCCGTATTAAACACCAAAACTTAACATATAGTTTACTTTGTGTTAAACAACAACAGTATGGCCGGGC
GCGGCAGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACAC
GGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCTGGGTGTGGTGGCGGGCGCCTGTAGTCCCAACTACTTGGGAGGCTGAGGCA
GGAGAATGGCTTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCAAGATCGCACCACTGCACTCCAGCCTGGTCGACAGCGAGACTCCATCT
CAAAAAAAAAAAAAAAATACCAGGTCTGGTTTTTGAAATTCTTTCTCCGTATTAAACACCAAAACTTAACATATAGTTTACTTTGTGTTA
AACAACAACAGTATGTTTTAAAGCAAGTATGTCTTTATTTGTAAGCATATTACTGAAACGATCAAATGAACAGAAGTGGATAAACGTCGA
GTCACTTGGCTGAGACCATACGCATCTTCCGTACTGCTTGTATTAGGATGCAAGATTGAATGTGTCTTTTTTTTAAAAATGCAGCCTTGT
GTTGCACTCTGAAATAAGTGATTTAAAAAGAAAAACATGATACAGAGATCCCATCCTGACCTTGGAGGCTTGGATAAAGCTGTGGGGAGA
CAGCCTCATGGTCCAGCCAGGCTAGTCCGCCTGCTTCAGGACTCTCAAGATCTCCCCAAGACTTTCAGGATCAGCTGCTGTTAATCAAAC
AAGTGCTTATAAAATGGAAATTGGGACAAAGGAAATGTTAAATTTCCTCCCTGCAACCAGGCAGATGAGAAATATGGAAATAAGGAAAAG
GGAAAATGAAGATATGACAAAGTTGATGAAAAGGTTGAAATAGTGATTTATACATGATGGGCAATCAAGTGCCCCTTTTGCCCTGTTTTT
TGGAGAGGGTGTATCATTGCCTCAATGGTGCGTCTGCCTGCACTGAGGGCATTACACATGGCTGACTCGACCTCCCTGCCTCTCACACTC
TGTGTATTTTGTGAAGCTCCACAAACGGGGTCACGTCATCCAGGTGAGGCCACCACCGGTACAGAAACCTCTCGGCAATGGTTCTTAGCC
AGGGGGTGACTTTGACTTCACCCCTCGCCTCCCTCTCCAGCAGCTCCCACAGCTCCTCCTGGGACAGGTAGAGGATGCTTTTCGTTTCAC
TGGGATCCGGGTTCAGAGTGACGTTTTTCCTCACAAGCAGAAGGTAACAAATTTCATGCTCTCCCCAAATTCTGTCTGATTTTGCCTTGT
GGTGATAGATTGTCATGAACACAATGTCCTCTGGAGAAATCTATTGACAGAAATTGGTGCAGTGTTAACAACGCTAATGTAAAACACAGA

>4965_4965_1_ANP32B-GTPBP4_ANP32B_chr9_100773593_ENST00000339399_GTPBP4_chr10_1063014_ENST00000360803_length(amino acids)=227AA_BP=
MPARRPPPRTAPPASAASKPFRRPSLRKPGRLSPLRPRRGKLSLKRGEEGNMDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTA
EFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLN

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Fusion Gene PPI Analysis for ANP32B-GTPBP4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ANP32B-GTPBP4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ANP32B-GTPBP4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource