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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:CNOT1-GOT2 (FusionGDB2 ID:HG23019TG2806)

Fusion Gene Summary for CNOT1-GOT2

Fusion gene summary

Fusion gene information	Fusion gene name: CNOT1-GOT2
	Fusion gene ID: hg23019tg2806
		Hgene	Tgene
	Gene symbol	CNOT1	GOT2
	Gene ID	23019	2806
	Gene name	CCR4-NOT transcription complex subunit 1	glutamic-oxaloacetic transaminase 2
	Synonyms	AD-005\|CDC39\|HPE12\|NOT1\|NOT1H	KAT4\|KATIV\|KYAT4\|mitAAT
	Cytomap	('CNOT1')('GOT2') 16q21	16q21
	Type of gene	protein-coding	protein-coding
	Description	CCR4-NOT transcription complex subunit 1CCR4-associated factor 1NOT1 (negative regulator of transcription 1, yeast) homologadrenal gland protein AD-005negative regulator of transcription subunit 1 homolog	aspartate aminotransferase, mitochondrialFABP-1FABPpmaspartate aminotransferase 2aspartate transaminase 2fatty acid-binding proteinglutamate oxaloacetate transaminase 2glutamic-oxaloacetic transaminase 2, mitochondrialkynurenine aminotransferase 4
	Modification date	20200322	20200313
	UniProtAcc	.	.
	Ensembl transtripts involved in fusion gene	ENST00000317147, ENST00000441024, ENST00000569240, ENST00000245138, ENST00000569732,
Fusion gene scores	* DoF score	19 X 17 X 11=3553	7 X 9 X 3=189
	# samples	21	9
	** MAII score	log2(21/3553*10)=-4.0805764556651 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/189*10)=-1.0703893278914 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: CNOT1 [Title/Abstract] AND GOT2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	CNOT1(58608513)-GOT2(58757806), # samples:2 CNOT1(58663632)-GOT2(58757806), # samples:2
Anticipated loss of major functional domain due to fusion event.	CNOT1-GOT2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CNOT1-GOT2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CNOT1-GOT2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CNOT1-GOT2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	CNOT1	GO:0000122	negative regulation of transcription by RNA polymerase II	16778766
Hgene	CNOT1	GO:0010606	positive regulation of cytoplasmic mRNA processing body assembly	21976065
Hgene	CNOT1	GO:0017148	negative regulation of translation	24736845
Hgene	CNOT1	GO:0033147	negative regulation of intracellular estrogen receptor signaling pathway	16778766
Hgene	CNOT1	GO:0035195	gene silencing by miRNA	23172285\|24768540
Hgene	CNOT1	GO:0048387	negative regulation of retinoic acid receptor signaling pathway	16778766
Hgene	CNOT1	GO:0060213	positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	23644599
Tgene	GOT2	GO:0006533	aspartate catabolic process	2567216
Tgene	GOT2	GO:0045471	response to ethanol	9537447

Fusion gene breakpoints across CNOT1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across GOT2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BLCA	TCGA-E7-A85H-01A	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
ChimerDB4	LUSC	TCGA-98-A539-01A	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
ChimerDB4	LUSC	TCGA-98-A539	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
ChimerDB4	LUSC	TCGA-98-A539	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
ChimerDB4	LUSC	TCGA-98-A539	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-

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Fusion Gene ORF analysis for CNOT1-GOT2

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-5UTR	ENST00000317147	ENST00000564400	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
5CDS-5UTR	ENST00000441024	ENST00000564400	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
5CDS-5UTR	ENST00000569240	ENST00000564400	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000317147	ENST00000245206	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000317147	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000317147	ENST00000434819	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000317147	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000441024	ENST00000245206	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000441024	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000441024	ENST00000434819	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000441024	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000569240	ENST00000245206	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000569240	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000569240	ENST00000434819	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-3CDS	ENST00000569240	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-5UTR	ENST00000317147	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
5UTR-5UTR	ENST00000317147	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
5UTR-5UTR	ENST00000317147	ENST00000564400	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-5UTR	ENST00000317147	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-5UTR	ENST00000317147	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
5UTR-5UTR	ENST00000441024	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
5UTR-5UTR	ENST00000441024	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
5UTR-5UTR	ENST00000441024	ENST00000564400	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-5UTR	ENST00000441024	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-5UTR	ENST00000441024	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
5UTR-5UTR	ENST00000569240	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
5UTR-5UTR	ENST00000569240	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
5UTR-5UTR	ENST00000569240	ENST00000564400	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
5UTR-5UTR	ENST00000569240	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
5UTR-5UTR	ENST00000569240	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
In-frame	ENST00000317147	ENST00000245206	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
In-frame	ENST00000317147	ENST00000434819	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
In-frame	ENST00000441024	ENST00000245206	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
In-frame	ENST00000441024	ENST00000434819	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
In-frame	ENST00000569240	ENST00000245206	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
In-frame	ENST00000569240	ENST00000434819	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000245138	ENST00000245206	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000245138	ENST00000245206	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000245138	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000245138	ENST00000434819	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000245138	ENST00000434819	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000245138	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000569732	ENST00000245206	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000569732	ENST00000245206	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000569732	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000569732	ENST00000434819	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000569732	ENST00000434819	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
intron-3CDS	ENST00000569732	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
intron-5UTR	ENST00000245138	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
intron-5UTR	ENST00000245138	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
intron-5UTR	ENST00000245138	ENST00000564400	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
intron-5UTR	ENST00000245138	ENST00000564400	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
intron-5UTR	ENST00000245138	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
intron-5UTR	ENST00000245138	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
intron-5UTR	ENST00000569732	ENST00000245206	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
intron-5UTR	ENST00000569732	ENST00000434819	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-
intron-5UTR	ENST00000569732	ENST00000564400	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-
intron-5UTR	ENST00000569732	ENST00000564400	CNOT1	chr16	58663631	-	GOT2	chr16	58757806	-
intron-5UTR	ENST00000569732	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58757806	-
intron-5UTR	ENST00000569732	ENST00000564400	CNOT1	chr16	58663632	-	GOT2	chr16	58768212	-

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000317147	CNOT1	chr16	58608513	-	ENST00000245206	GOT2	chr16	58757806	-	4556	2312	312	3515	1067
ENST00000317147	CNOT1	chr16	58608513	-	ENST00000434819	GOT2	chr16	58757806	-	3740	2312	312	3386	1024
ENST00000569240	CNOT1	chr16	58608513	-	ENST00000245206	GOT2	chr16	58757806	-	4504	2260	260	3463	1067
ENST00000569240	CNOT1	chr16	58608513	-	ENST00000434819	GOT2	chr16	58757806	-	3688	2260	260	3334	1024
ENST00000441024	CNOT1	chr16	58608513	-	ENST00000245206	GOT2	chr16	58757806	-	4508	2264	264	3467	1067
ENST00000441024	CNOT1	chr16	58608513	-	ENST00000434819	GOT2	chr16	58757806	-	3692	2264	264	3338	1024

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000317147	ENST00000245206	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-	0.001280504	0.9987196
ENST00000317147	ENST00000434819	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-	0.001371727	0.99862826
ENST00000569240	ENST00000245206	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-	0.001224283	0.99877566
ENST00000569240	ENST00000434819	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-	0.001309767	0.9986902
ENST00000441024	ENST00000245206	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-	0.001229852	0.9987702
ENST00000441024	ENST00000434819	CNOT1	chr16	58608513	-	GOT2	chr16	58757806	-	0.001312599	0.99868745

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Fusion Genomic Features for CNOT1-GOT2

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

1-p

p (fusion gene breakpoint)

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

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Fusion Protein Features for CNOT1-GOT2

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr16:58608513/chr16:58757806)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.	FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000317147

153_157

659

2377.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000317147

181_185

659

2377.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000317147

223_227

659

2377.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000317147

570_574

659

2377.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000441024

153_157

659

1552.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000441024

181_185

659

1552.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000441024

223_227

659

1552.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000441024

570_574

659

1552.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000569240

153_157

659

2372.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000569240

181_185

659

2372.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000569240

223_227

659

2372.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000569240

570_574

659

2372.0

Motif

Note=LXXLL

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000317147

1333_1350

659

2377.0

Compositional bias

Note=Thr-rich

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000441024

1333_1350

659

1552.0

Compositional bias

Note=Thr-rich

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000569240

1333_1350

659

2372.0

Compositional bias

Note=Thr-rich

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000317147

1639_1643

659

2377.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000317147

1942_1946

659

2377.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000317147

2096_2100

659

2377.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000441024

1639_1643

659

1552.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000441024

1942_1946

659

1552.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000441024

2096_2100

659

1552.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000569240

1639_1643

659

2372.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000569240

1942_1946

659

2372.0

Motif

Note=LXXLL

Hgene

CNOT1

chr16:58608513

chr16:58757806

ENST00000569240

2096_2100

659

2372.0

Motif

Note=LXXLL

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Fusion Gene Sequence for CNOT1-GOT2

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>17712_17712_1_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000317147_GOT2_chr16_58757806_ENST00000245206_length(transcript)=4556nt_BP=2312nt
GAAATGGGAACGGGGAGGGGAAAGGCCGCGACGTGAGCGCGCGACCTCCGGCGCCATTTTGTAGAGAAACAAGCGGAGTTAACCGAAGAG
GGGGTCGAGGAGAGCCGGAGTCGGGGACCCAGGAGTTTCCTGTGTCCAGCGCTGCCGGAGCCGCCTGAGGTGCCATGTTTCAGAACAGAG
TAAGACCCCTGGTAAAGAAGAACTGAAGATATTATACAGATACCAGATATAGCCTAATTACAAAGAAAGCATTAACCTGCCTCTGAGGTG
ACTAAAGGGGAATAATGGTGATTTTGCGCCGGGCTCGGCCGCCTGCTTCCGCCCCAACCAGCAATGAATCTTGACTCGCTCTCGCTGGCC
TTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAAATTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCAC
GGTCCTGAGGCAGACAGGCATTTATTACGCTGCCTATTTTCGCATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCAT
CAGACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCAAATTTTATCTCGACGCTGTCCTATGCCATTGATAATCCATTG
CACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAAAGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTT
GGCCTTGCCCTGTTGAATTCTTCCAGCTCAGATCTTAGAGGTTTCGCTGCCCAGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCT
TACATTGACGCAGACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTCCTCTCCCATCTCCTC
TTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCTTTTCTTAAGACGCTGCGCAGAGATTTTCCCCAAGAACGCTGT
CCCGTGGTGCTCGCACCACTTTTATACCCTGAAAAACGGGACATTCTAATGGACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACC
ATGATGGAGAGCTCTTTGGCTGATTTCATGCAAGAAGTAGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCGTGCAG
TTTGGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGAATGATGGCTCGAACTCATTCAGGATTAACAGATGGCATTCCA
TTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGTGATGGGAAAGATAAAAGTGATGGAGCACAGGCACACACATGGAATGTAGAA
GTCTTGATTGACGTTCTTAAAGAACTGAATCCAAGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAAATTCGT
GACAGTAAAGGACTTCATAATGTGGTTTATGGCATTCAGAGGGGTTTGGGTATGGAAGTGTTCCCAGTAGACCTCATATATAGACCTTGG
AAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATAAATCCAGAGATCTTCTGTTTTGCTGACTATCCCTGTCATACTGTT
GCCACTGATATTCTGAAAGCACCACCAGAGGATGACAATCGAGAAATTGCCACATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGG
CTTGCAGAGGTTGGGCAGTATGAGCAAGTCAAACAGCTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGCTGGTATTGGCCTTACTA
CAAATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACTCTGATGCCAATTTTCCTTGGAAACCATCCTAACTCAGCTATT
ATTTTGCACTATGCATGGCATGGGCAGGGACAGTCTCCCTCAATTCGCCAACTTATCATGCATGCAATGGCAGAATGGTACATGAGAGGG
GAGCAGTATGATCAGGCCAAATTGTCTCGAATACTTGATGTGGCCCAGGACTTGAAGGCCTTGTCAATGCTGCTAAATGGTACTCCATTT
GCCTTTGTTATTGACCTTGCTGCACTTGCTTCACGTCGTGAATACCTCAAACTTGATAAGTGGCTCACAGATAAAATTCGAGAGCATGGG
GAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAGAGACGGTGTCCTTCTATTTTGGGCGGACTTGCCCCAGAAAAAGACCAGCCCAAA
AGTGCTCAACTTCCTCCAGAAACTTTGGCGACAATGTTGGCCTGTCTGCAAGCTTGTGCAGGCTCCTGGTGGACCCATGTGGAAATGGGA
CCTCCAGATCCCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACCAATAGCAAAAAGATGAATCTGGGAGTTGGTGCCTACCGGGAT
GATAATGGAAAGCCTTACGTTCTGCCTAGCGTCCGCAAGGCAGAGGCCCAGATTGCCGCAAAAAATTTGGACAAGGAATACCTGCCCATT
GGGGGACTGGCTGAATTTTGCAAGGCATCTGCAGAACTAGCCCTGGGTGAGAACAGCGAAGTCTTGAAGAGTGGCCGGTTTGTCACTGTG
CAGACCATTTCTGGAACTGGAGCCTTAAGGATCGGAGCCAGTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAA
CCAACCTGGGGAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGGTATTATGACCCCAAGACTTGCGGTTTT
GACTTCACAGGCGCTGTGGAGGATATTTCAAAAATACCAGAGCAGAGTGTTCTTCTTCTGCATGCCTGCGCCCACAATCCCACGGGAGTG
GACCCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTGGTGAAGAAAAGGAATCTCTTTGCGTTCTTTGACATGGCCTACCAAGGCTTT
GCCAGTGGTGATGGTGATAAGGATGCCTGGGCTGTGCGCCACTTCATCGAACAGGGCATTAATGTTTGCCTCTGCCAATCATATGCCAAG
AACATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGCAAAGATGCGGATGAAGCCAAAAGGGTAGAGTCACAGTTGAAG
ATCTTGATCCGTCCCATGTATTCCAACCCTCCCCTCAATGGGGCCCGGATTGCTGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAA
TGGCTGCAAGAAGTGAAAGTCATGGCTGACCGCATCATTGGCATGCGGACTCAACTGGTCTCCAACCTCAAGAAGGAGGGTTCCACCCAC
AATTGGCAACACATCACCGACCAAATTGGCATGTTCTGTTTCACAGGGCTAAAGCCTGAACAGGTGGAGCGGCTGATCAAGGAGTTCTCC
ATCTACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCCAGCAACGTGGGCTACCTTGCCCATGCCATTCACCAGGTCACC
AAGTAATGTCCCTGGTGCGAGGAAACAGAGACAACCTTTCTGTCTTCAGCCTCTGCTATTGAGAGCTTCACACAGACAATGAGAGAGGGT
GGATGGTGGTGAGTGGATCATTTCTTTCAGCCACAGTGTGTAACACTCAGCATTTGAATGTTTCTCAGAAAAGAACATGTAGTGACACAG
GGCAGAGGCATCCATGGCTGGCGTCTGGAATATTAAACCAAACTCTCCCCGGTCCTTTTTTCTCCAACTTTTCTCAAAGAGTTTACATGT
GCAAGAAAGTCATCGCACCAAAAAACCTGTCAATTATGCCATTGCAATATTTCAGAAGCTTTAACTGAAGTGTCAGGTTCCTCGTGAGAA
ACAGCACACGTTAGAGGCTTTGAGAGAAGGCCTAGTTCTGTCATGAGTAGTCGGCCTCGTGTCTGTCCTCCCATCTTGGAACAACCTTAT
CAACAGGCCGCACTGCAGAAATGATGTTTTATGAAAACCAATGAGGCTGCTGCCACTCCAGCAAGGGAAATAATGCAGTTTCCTGTCTTA
TTTAAGAAAAAGAGAAGGCTCTCTTTTCTCCCTTGTCATTGCCGTTCTTTTCCTTACACGCAAAGATTTTTTAACTATTGCAGATTTTCA
TCCCATTCTACTGCTTGATTGACCATCAACTCCATCCTATCGAGATTTATTTAAGAATGAAGAACATAATTTTCTGCTGATGCTGTACCC
TCACCCTTTTCAGCAAAGAATAGTGGAGAGTAGGAAACTGTACTTTATCTCGGCATCCTCTTGAATGATAGTGCAAGTTTCTCCAGTTGG
GATGTTGTCTCTGCCCGGTTGGACCTCCTCCCTTTGTTGAATGTGGTGTGCAGCCTCTCATCTCACACTGTGAGTCCAGCGGCGCAGGGT
GGTACCAGGAAAGAGGATATTCTAGGCTTTGCGTGCTGCTAGCTGGGTTCAGGCTTCACCCACTGGAAAGAACCACCATCTGCTCTAACC
ATGTAGACTTATTGCGGCCTGGTTTCTCTGTTACAATAAAATTACTGTAGACCCAA

>17712_17712_1_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000317147_GOT2_chr16_58757806_ENST00000245206_length(amino acids)=1067AA_BP=667
MLPPQPAMNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLIT
KPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGF
QDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVG
YGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLN
FKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNR
EIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPS
IRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRR
CPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKA
EAQIAAKNLDKEYLPIGGLAEFCKASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAG
MQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRH
FIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVESQLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIG
MRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK

--------------------------------------------------------------
>17712_17712_2_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000317147_GOT2_chr16_58757806_ENST00000434819_length(transcript)=3740nt_BP=2312nt
GAAATGGGAACGGGGAGGGGAAAGGCCGCGACGTGAGCGCGCGACCTCCGGCGCCATTTTGTAGAGAAACAAGCGGAGTTAACCGAAGAG
GGGGTCGAGGAGAGCCGGAGTCGGGGACCCAGGAGTTTCCTGTGTCCAGCGCTGCCGGAGCCGCCTGAGGTGCCATGTTTCAGAACAGAG
TAAGACCCCTGGTAAAGAAGAACTGAAGATATTATACAGATACCAGATATAGCCTAATTACAAAGAAAGCATTAACCTGCCTCTGAGGTG
ACTAAAGGGGAATAATGGTGATTTTGCGCCGGGCTCGGCCGCCTGCTTCCGCCCCAACCAGCAATGAATCTTGACTCGCTCTCGCTGGCC
TTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAAATTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCAC
GGTCCTGAGGCAGACAGGCATTTATTACGCTGCCTATTTTCGCATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCAT
CAGACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCAAATTTTATCTCGACGCTGTCCTATGCCATTGATAATCCATTG
CACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAAAGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTT
GGCCTTGCCCTGTTGAATTCTTCCAGCTCAGATCTTAGAGGTTTCGCTGCCCAGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCT
TACATTGACGCAGACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTCCTCTCCCATCTCCTC
TTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCTTTTCTTAAGACGCTGCGCAGAGATTTTCCCCAAGAACGCTGT
CCCGTGGTGCTCGCACCACTTTTATACCCTGAAAAACGGGACATTCTAATGGACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACC
ATGATGGAGAGCTCTTTGGCTGATTTCATGCAAGAAGTAGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCGTGCAG
TTTGGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGAATGATGGCTCGAACTCATTCAGGATTAACAGATGGCATTCCA
TTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGTGATGGGAAAGATAAAAGTGATGGAGCACAGGCACACACATGGAATGTAGAA
GTCTTGATTGACGTTCTTAAAGAACTGAATCCAAGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAAATTCGT
GACAGTAAAGGACTTCATAATGTGGTTTATGGCATTCAGAGGGGTTTGGGTATGGAAGTGTTCCCAGTAGACCTCATATATAGACCTTGG
AAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATAAATCCAGAGATCTTCTGTTTTGCTGACTATCCCTGTCATACTGTT
GCCACTGATATTCTGAAAGCACCACCAGAGGATGACAATCGAGAAATTGCCACATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGG
CTTGCAGAGGTTGGGCAGTATGAGCAAGTCAAACAGCTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGCTGGTATTGGCCTTACTA
CAAATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACTCTGATGCCAATTTTCCTTGGAAACCATCCTAACTCAGCTATT
ATTTTGCACTATGCATGGCATGGGCAGGGACAGTCTCCCTCAATTCGCCAACTTATCATGCATGCAATGGCAGAATGGTACATGAGAGGG
GAGCAGTATGATCAGGCCAAATTGTCTCGAATACTTGATGTGGCCCAGGACTTGAAGGCCTTGTCAATGCTGCTAAATGGTACTCCATTT
GCCTTTGTTATTGACCTTGCTGCACTTGCTTCACGTCGTGAATACCTCAAACTTGATAAGTGGCTCACAGATAAAATTCGAGAGCATGGG
GAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAGAGACGGTGTCCTTCTATTTTGGGCGGACTTGCCCCAGAAAAAGACCAGCCCAAA
AGTGCTCAACTTCCTCCAGAAACTTTGGCGACAATGTTGGCCTGTCTGCAAGCTTGTGCAGGCTCCTGGTGGACCCATGTGGAAATGGGA
CCTCCAGATCCCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACCAATAGCAAAAAGATGAATCTGGGAGTTGGTGCCTACCGGGAT
GATAATGGAAAGCCTTACGTTCTGCCTAGCGTCCGCAAGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATCGGAGCC
AGTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACCAACCTGGGGAAACCACACACCCATCTTCAGGGATGCT
GGCATGCAGCTACAAGGTTATCGGTATTATGACCCCAAGACTTGCGGTTTTGACTTCACAGGCGCTGTGGAGGATATTTCAAAAATACCA
GAGCAGAGTGTTCTTCTTCTGCATGCCTGCGCCCACAATCCCACGGGAGTGGACCCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTG
GTGAAGAAAAGGAATCTCTTTGCGTTCTTTGACATGGCCTACCAAGGCTTTGCCAGTGGTGATGGTGATAAGGATGCCTGGGCTGTGCGC
CACTTCATCGAACAGGGCATTAATGTTTGCCTCTGCCAATCATATGCCAAGAACATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACT
ATGGTCTGCAAAGATGCGGATGAAGCCAAAAGGGTAGAGTCACAGTTGAAGATCTTGATCCGTCCCATGTATTCCAACCCTCCCCTCAAT
GGGGCCCGGATTGCTGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAATGGCTGCAAGAAGTGAAAGTCATGGCTGACCGCATCATT
GGCATGCGGACTCAACTGGTCTCCAACCTCAAGAAGGAGGGTTCCACCCACAATTGGCAACACATCACCGACCAAATTGGCATGTTCTGT
TTCACAGGGCTAAAGCCTGAACAGGTGGAGCGGCTGATCAAGGAGTTCTCCATCTACATGACAAAAGATGGCCGCATCTCTGTGGCAGGG
GTCACCTCCAGCAACGTGGGCTACCTTGCCCATGCCATTCACCAGGTCACCAAGTAATGTCCCTGGTGCGAGGAAACAGAGACAACCTTT
CTGTCTTCAGCCTCTGCTATTGAGAGCTTCACACAGACAATGAGAGAGGGTGGATGGTGGTGAGTGGATCATTTCTTTCAGCCACAGTGT
GTAACACTCAGCATTTGAATGTTTCTCAGAAAAGAACATGTAGTGACACAGGGCAGAGGCATCCATGGCTGGCGTCTGGAATATTAAACC
AAACTCTCCCCGGTCCTTTTTTCTCCAACTTTTCTCAAAGAGTTTACATGTGCAAGAAAGTCATCGCACCAAAAAACCTGTCAATTATGC
CATTGCAATATTTCAGAAGCTTTAACTGAAGTGTCAGGTTCCTCGTGAGA

>17712_17712_2_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000317147_GOT2_chr16_58757806_ENST00000434819_length(amino acids)=1024AA_BP=667
MLPPQPAMNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLIT
KPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGF
QDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVG
YGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLN
FKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNR
EIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPS
IRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRR
CPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKF
VTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNP
TGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVES
QLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIK
EFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK

--------------------------------------------------------------
>17712_17712_3_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000441024_GOT2_chr16_58757806_ENST00000245206_length(transcript)=4508nt_BP=2264nt
CGGCGCCATTTTGTAGAGAAACAAGCGGAGTTAACCGAAGAGGGGGTCGAGGAGAGCCGGAGTCGGGGACCCAGGAGTTTCCTGTGTCCA
GCGCTGCCGGAGCCGCCTGAGGTGCCATGTTTCAGAACAGAGTAAGACCCCTGGTAAAGAAGAACTGAAGATATTATACAGATACCAGAT
ATAGCCTAATTACAAAGAAAGCATTAACCTGCCTCTGAGGTGACTAAAGGGGAATAATGGTGATTTTGCGCCGGGCTCGGCCGCCTGCTT
CCGCCCCAACCAGCAATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAAATTAC
CGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTTATTACGCTGCCTATTTTCGCATGTG
GATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAGACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCA
AATTTTATCTCGACGCTGTCCTATGCCATTGATAATCCATTGCACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTG
AGTAAAGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTTGGCCTTGCCCTGTTGAATTCTTCCAGCTCAGATCTTAGAGGTTTCGCT
GCCCAGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCTTACATTGACGCAGACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGAT
ATAGCAATAGAGGTCCTACACCTCCTCCTCTCCCATCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCT
TTTCTTAAGACGCTGCGCAGAGATTTTCCCCAAGAACGCTGTCCCGTGGTGCTCGCACCACTTTTATACCCTGAAAAACGGGACATTCTA
ATGGACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACCATGATGGAGAGCTCTTTGGCTGATTTCATGCAAGAAGTAGGCTATGGC
TTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCGTGCAGTTTGGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGA
ATGATGGCTCGAACTCATTCAGGATTAACAGATGGCATTCCATTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGTGATGGGAAA
GATAAAAGTGATGGAGCACAGGCACACACATGGAATGTAGAAGTCTTGATTGACGTTCTTAAAGAACTGAATCCAAGTTTGAATTTCAAG
GAAGTAACTTATGAACTGGACCATCCTGGATTTCAAATTCGTGACAGTAAAGGACTTCATAATGTGGTTTATGGCATTCAGAGGGGTTTG
GGTATGGAAGTGTTCCCAGTAGACCTCATATATAGACCTTGGAAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATAAAT
CCAGAGATCTTCTGTTTTGCTGACTATCCCTGTCATACTGTTGCCACTGATATTCTGAAAGCACCACCAGAGGATGACAATCGAGAAATT
GCCACATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGAGCAAGTCAAACAGCTCTTCAGCTTC
CCTATCAAACACTGTCCAGACATGCTGGTATTGGCCTTACTACAAATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACT
CTGATGCCAATTTTCCTTGGAAACCATCCTAACTCAGCTATTATTTTGCACTATGCATGGCATGGGCAGGGACAGTCTCCCTCAATTCGC
CAACTTATCATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATACTTGATGTGGCCCAG
GACTTGAAGGCCTTGTCAATGCTGCTAAATGGTACTCCATTTGCCTTTGTTATTGACCTTGCTGCACTTGCTTCACGTCGTGAATACCTC
AAACTTGATAAGTGGCTCACAGATAAAATTCGAGAGCATGGGGAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAGAGACGGTGTCCT
TCTATTTTGGGCGGACTTGCCCCAGAAAAAGACCAGCCCAAAAGTGCTCAACTTCCTCCAGAAACTTTGGCGACAATGTTGGCCTGTCTG
CAAGCTTGTGCAGGCTCCTGGTGGACCCATGTGGAAATGGGACCTCCAGATCCCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACC
AATAGCAAAAAGATGAATCTGGGAGTTGGTGCCTACCGGGATGATAATGGAAAGCCTTACGTTCTGCCTAGCGTCCGCAAGGCAGAGGCC
CAGATTGCCGCAAAAAATTTGGACAAGGAATACCTGCCCATTGGGGGACTGGCTGAATTTTGCAAGGCATCTGCAGAACTAGCCCTGGGT
GAGAACAGCGAAGTCTTGAAGAGTGGCCGGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATCGGAGCCAGTTTTCTG
CAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACCAACCTGGGGAAACCACACACCCATCTTCAGGGATGCTGGCATGCAG
CTACAAGGTTATCGGTATTATGACCCCAAGACTTGCGGTTTTGACTTCACAGGCGCTGTGGAGGATATTTCAAAAATACCAGAGCAGAGT
GTTCTTCTTCTGCATGCCTGCGCCCACAATCCCACGGGAGTGGACCCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTGGTGAAGAAA
AGGAATCTCTTTGCGTTCTTTGACATGGCCTACCAAGGCTTTGCCAGTGGTGATGGTGATAAGGATGCCTGGGCTGTGCGCCACTTCATC
GAACAGGGCATTAATGTTTGCCTCTGCCAATCATATGCCAAGAACATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGC
AAAGATGCGGATGAAGCCAAAAGGGTAGAGTCACAGTTGAAGATCTTGATCCGTCCCATGTATTCCAACCCTCCCCTCAATGGGGCCCGG
ATTGCTGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAATGGCTGCAAGAAGTGAAAGTCATGGCTGACCGCATCATTGGCATGCGG
ACTCAACTGGTCTCCAACCTCAAGAAGGAGGGTTCCACCCACAATTGGCAACACATCACCGACCAAATTGGCATGTTCTGTTTCACAGGG
CTAAAGCCTGAACAGGTGGAGCGGCTGATCAAGGAGTTCTCCATCTACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCC
AGCAACGTGGGCTACCTTGCCCATGCCATTCACCAGGTCACCAAGTAATGTCCCTGGTGCGAGGAAACAGAGACAACCTTTCTGTCTTCA
GCCTCTGCTATTGAGAGCTTCACACAGACAATGAGAGAGGGTGGATGGTGGTGAGTGGATCATTTCTTTCAGCCACAGTGTGTAACACTC
AGCATTTGAATGTTTCTCAGAAAAGAACATGTAGTGACACAGGGCAGAGGCATCCATGGCTGGCGTCTGGAATATTAAACCAAACTCTCC
CCGGTCCTTTTTTCTCCAACTTTTCTCAAAGAGTTTACATGTGCAAGAAAGTCATCGCACCAAAAAACCTGTCAATTATGCCATTGCAAT
ATTTCAGAAGCTTTAACTGAAGTGTCAGGTTCCTCGTGAGAAACAGCACACGTTAGAGGCTTTGAGAGAAGGCCTAGTTCTGTCATGAGT
AGTCGGCCTCGTGTCTGTCCTCCCATCTTGGAACAACCTTATCAACAGGCCGCACTGCAGAAATGATGTTTTATGAAAACCAATGAGGCT
GCTGCCACTCCAGCAAGGGAAATAATGCAGTTTCCTGTCTTATTTAAGAAAAAGAGAAGGCTCTCTTTTCTCCCTTGTCATTGCCGTTCT
TTTCCTTACACGCAAAGATTTTTTAACTATTGCAGATTTTCATCCCATTCTACTGCTTGATTGACCATCAACTCCATCCTATCGAGATTT
ATTTAAGAATGAAGAACATAATTTTCTGCTGATGCTGTACCCTCACCCTTTTCAGCAAAGAATAGTGGAGAGTAGGAAACTGTACTTTAT
CTCGGCATCCTCTTGAATGATAGTGCAAGTTTCTCCAGTTGGGATGTTGTCTCTGCCCGGTTGGACCTCCTCCCTTTGTTGAATGTGGTG
TGCAGCCTCTCATCTCACACTGTGAGTCCAGCGGCGCAGGGTGGTACCAGGAAAGAGGATATTCTAGGCTTTGCGTGCTGCTAGCTGGGT
TCAGGCTTCACCCACTGGAAAGAACCACCATCTGCTCTAACCATGTAGACTTATTGCGGCCTGGTTTCTCTGTTACAATAAAATTACTGT
AGACCCAA

>17712_17712_3_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000441024_GOT2_chr16_58757806_ENST00000245206_length(amino acids)=1067AA_BP=667
MLPPQPAMNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLIT
KPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGF
QDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVG
YGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLN
FKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNR
EIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPS
IRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRR
CPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKA
EAQIAAKNLDKEYLPIGGLAEFCKASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAG
MQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRH
FIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVESQLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIG
MRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK

--------------------------------------------------------------
>17712_17712_4_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000441024_GOT2_chr16_58757806_ENST00000434819_length(transcript)=3692nt_BP=2264nt
CGGCGCCATTTTGTAGAGAAACAAGCGGAGTTAACCGAAGAGGGGGTCGAGGAGAGCCGGAGTCGGGGACCCAGGAGTTTCCTGTGTCCA
GCGCTGCCGGAGCCGCCTGAGGTGCCATGTTTCAGAACAGAGTAAGACCCCTGGTAAAGAAGAACTGAAGATATTATACAGATACCAGAT
ATAGCCTAATTACAAAGAAAGCATTAACCTGCCTCTGAGGTGACTAAAGGGGAATAATGGTGATTTTGCGCCGGGCTCGGCCGCCTGCTT
CCGCCCCAACCAGCAATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAAATTAC
CGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTTATTACGCTGCCTATTTTCGCATGTG
GATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAGACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCA
AATTTTATCTCGACGCTGTCCTATGCCATTGATAATCCATTGCACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTG
AGTAAAGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTTGGCCTTGCCCTGTTGAATTCTTCCAGCTCAGATCTTAGAGGTTTCGCT
GCCCAGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCTTACATTGACGCAGACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGAT
ATAGCAATAGAGGTCCTACACCTCCTCCTCTCCCATCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCT
TTTCTTAAGACGCTGCGCAGAGATTTTCCCCAAGAACGCTGTCCCGTGGTGCTCGCACCACTTTTATACCCTGAAAAACGGGACATTCTA
ATGGACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACCATGATGGAGAGCTCTTTGGCTGATTTCATGCAAGAAGTAGGCTATGGC
TTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCGTGCAGTTTGGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGA
ATGATGGCTCGAACTCATTCAGGATTAACAGATGGCATTCCATTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGTGATGGGAAA
GATAAAAGTGATGGAGCACAGGCACACACATGGAATGTAGAAGTCTTGATTGACGTTCTTAAAGAACTGAATCCAAGTTTGAATTTCAAG
GAAGTAACTTATGAACTGGACCATCCTGGATTTCAAATTCGTGACAGTAAAGGACTTCATAATGTGGTTTATGGCATTCAGAGGGGTTTG
GGTATGGAAGTGTTCCCAGTAGACCTCATATATAGACCTTGGAAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATAAAT
CCAGAGATCTTCTGTTTTGCTGACTATCCCTGTCATACTGTTGCCACTGATATTCTGAAAGCACCACCAGAGGATGACAATCGAGAAATT
GCCACATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGAGCAAGTCAAACAGCTCTTCAGCTTC
CCTATCAAACACTGTCCAGACATGCTGGTATTGGCCTTACTACAAATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACT
CTGATGCCAATTTTCCTTGGAAACCATCCTAACTCAGCTATTATTTTGCACTATGCATGGCATGGGCAGGGACAGTCTCCCTCAATTCGC
CAACTTATCATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATACTTGATGTGGCCCAG
GACTTGAAGGCCTTGTCAATGCTGCTAAATGGTACTCCATTTGCCTTTGTTATTGACCTTGCTGCACTTGCTTCACGTCGTGAATACCTC
AAACTTGATAAGTGGCTCACAGATAAAATTCGAGAGCATGGGGAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAGAGACGGTGTCCT
TCTATTTTGGGCGGACTTGCCCCAGAAAAAGACCAGCCCAAAAGTGCTCAACTTCCTCCAGAAACTTTGGCGACAATGTTGGCCTGTCTG
CAAGCTTGTGCAGGCTCCTGGTGGACCCATGTGGAAATGGGACCTCCAGATCCCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACC
AATAGCAAAAAGATGAATCTGGGAGTTGGTGCCTACCGGGATGATAATGGAAAGCCTTACGTTCTGCCTAGCGTCCGCAAGTTTGTCACT
GTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATCGGAGCCAGTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCC
AAACCAACCTGGGGAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGGTATTATGACCCCAAGACTTGCGGT
TTTGACTTCACAGGCGCTGTGGAGGATATTTCAAAAATACCAGAGCAGAGTGTTCTTCTTCTGCATGCCTGCGCCCACAATCCCACGGGA
GTGGACCCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTGGTGAAGAAAAGGAATCTCTTTGCGTTCTTTGACATGGCCTACCAAGGC
TTTGCCAGTGGTGATGGTGATAAGGATGCCTGGGCTGTGCGCCACTTCATCGAACAGGGCATTAATGTTTGCCTCTGCCAATCATATGCC
AAGAACATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGCAAAGATGCGGATGAAGCCAAAAGGGTAGAGTCACAGTTG
AAGATCTTGATCCGTCCCATGTATTCCAACCCTCCCCTCAATGGGGCCCGGATTGCTGCTGCCATTCTGAACACCCCAGATTTGCGAAAA
CAATGGCTGCAAGAAGTGAAAGTCATGGCTGACCGCATCATTGGCATGCGGACTCAACTGGTCTCCAACCTCAAGAAGGAGGGTTCCACC
CACAATTGGCAACACATCACCGACCAAATTGGCATGTTCTGTTTCACAGGGCTAAAGCCTGAACAGGTGGAGCGGCTGATCAAGGAGTTC
TCCATCTACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCCAGCAACGTGGGCTACCTTGCCCATGCCATTCACCAGGTC
ACCAAGTAATGTCCCTGGTGCGAGGAAACAGAGACAACCTTTCTGTCTTCAGCCTCTGCTATTGAGAGCTTCACACAGACAATGAGAGAG
GGTGGATGGTGGTGAGTGGATCATTTCTTTCAGCCACAGTGTGTAACACTCAGCATTTGAATGTTTCTCAGAAAAGAACATGTAGTGACA
CAGGGCAGAGGCATCCATGGCTGGCGTCTGGAATATTAAACCAAACTCTCCCCGGTCCTTTTTTCTCCAACTTTTCTCAAAGAGTTTACA
TGTGCAAGAAAGTCATCGCACCAAAAAACCTGTCAATTATGCCATTGCAATATTTCAGAAGCTTTAACTGAAGTGTCAGGTTCCTCGTGA
GA

>17712_17712_4_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000441024_GOT2_chr16_58757806_ENST00000434819_length(amino acids)=1024AA_BP=667
MLPPQPAMNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLIT
KPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGF
QDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVG
YGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLN
FKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNR
EIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPS
IRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRR
CPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKF
VTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNP
TGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVES
QLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIK
EFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK

--------------------------------------------------------------
>17712_17712_5_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000569240_GOT2_chr16_58757806_ENST00000245206_length(transcript)=4504nt_BP=2260nt
GCCATTTTGTAGAGAAACAAGCGGAGTTAACCGAAGAGGGGGTCGAGGAGAGCCGGAGTCGGGGACCCAGGAGTTTCCTGTGTCCAGCGC
TGCCGGAGCCGCCTGAGGTGCCATGTTTCAGAACAGAGTAAGACCCCTGGTAAAGAAGAACTGAAGATATTATACAGATACCAGATATAG
CCTAATTACAAAGAAAGCATTAACCTGCCTCTGAGGTGACTAAAGGGGAATAATGGTGATTTTGCGCCGGGCTCGGCCGCCTGCTTCCGC
CCCAACCAGCAATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAAATTACCGAG
CCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTTATTACGCTGCCTATTTTCGCATGTGGATT
TCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAGACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCAAATT
TTATCTCGACGCTGTCCTATGCCATTGATAATCCATTGCACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTA
AAGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTTGGCCTTGCCCTGTTGAATTCTTCCAGCTCAGATCTTAGAGGTTTCGCTGCCC
AGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCTTACATTGACGCAGACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAG
CAATAGAGGTCCTACACCTCCTCCTCTCCCATCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCTTTTC
TTAAGACGCTGCGCAGAGATTTTCCCCAAGAACGCTGTCCCGTGGTGCTCGCACCACTTTTATACCCTGAAAAACGGGACATTCTAATGG
ACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACCATGATGGAGAGCTCTTTGGCTGATTTCATGCAAGAAGTAGGCTATGGCTTTT
GTGCAAGTATTGAAGAATGTCGCAATATAATCGTGCAGTTTGGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGAATGA
TGGCTCGAACTCATTCAGGATTAACAGATGGCATTCCATTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGTGATGGGAAAGATA
AAAGTGATGGAGCACAGGCACACACATGGAATGTAGAAGTCTTGATTGACGTTCTTAAAGAACTGAATCCAAGTTTGAATTTCAAGGAAG
TAACTTATGAACTGGACCATCCTGGATTTCAAATTCGTGACAGTAAAGGACTTCATAATGTGGTTTATGGCATTCAGAGGGGTTTGGGTA
TGGAAGTGTTCCCAGTAGACCTCATATATAGACCTTGGAAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATAAATCCAG
AGATCTTCTGTTTTGCTGACTATCCCTGTCATACTGTTGCCACTGATATTCTGAAAGCACCACCAGAGGATGACAATCGAGAAATTGCCA
CATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGAGCAAGTCAAACAGCTCTTCAGCTTCCCTA
TCAAACACTGTCCAGACATGCTGGTATTGGCCTTACTACAAATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACTCTGA
TGCCAATTTTCCTTGGAAACCATCCTAACTCAGCTATTATTTTGCACTATGCATGGCATGGGCAGGGACAGTCTCCCTCAATTCGCCAAC
TTATCATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATACTTGATGTGGCCCAGGACT
TGAAGGCCTTGTCAATGCTGCTAAATGGTACTCCATTTGCCTTTGTTATTGACCTTGCTGCACTTGCTTCACGTCGTGAATACCTCAAAC
TTGATAAGTGGCTCACAGATAAAATTCGAGAGCATGGGGAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAGAGACGGTGTCCTTCTA
TTTTGGGCGGACTTGCCCCAGAAAAAGACCAGCCCAAAAGTGCTCAACTTCCTCCAGAAACTTTGGCGACAATGTTGGCCTGTCTGCAAG
CTTGTGCAGGCTCCTGGTGGACCCATGTGGAAATGGGACCTCCAGATCCCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACCAATA
GCAAAAAGATGAATCTGGGAGTTGGTGCCTACCGGGATGATAATGGAAAGCCTTACGTTCTGCCTAGCGTCCGCAAGGCAGAGGCCCAGA
TTGCCGCAAAAAATTTGGACAAGGAATACCTGCCCATTGGGGGACTGGCTGAATTTTGCAAGGCATCTGCAGAACTAGCCCTGGGTGAGA
ACAGCGAAGTCTTGAAGAGTGGCCGGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATCGGAGCCAGTTTTCTGCAAA
GATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACCAACCTGGGGAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTAC
AAGGTTATCGGTATTATGACCCCAAGACTTGCGGTTTTGACTTCACAGGCGCTGTGGAGGATATTTCAAAAATACCAGAGCAGAGTGTTC
TTCTTCTGCATGCCTGCGCCCACAATCCCACGGGAGTGGACCCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTGGTGAAGAAAAGGA
ATCTCTTTGCGTTCTTTGACATGGCCTACCAAGGCTTTGCCAGTGGTGATGGTGATAAGGATGCCTGGGCTGTGCGCCACTTCATCGAAC
AGGGCATTAATGTTTGCCTCTGCCAATCATATGCCAAGAACATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGCAAAG
ATGCGGATGAAGCCAAAAGGGTAGAGTCACAGTTGAAGATCTTGATCCGTCCCATGTATTCCAACCCTCCCCTCAATGGGGCCCGGATTG
CTGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAATGGCTGCAAGAAGTGAAAGTCATGGCTGACCGCATCATTGGCATGCGGACTC
AACTGGTCTCCAACCTCAAGAAGGAGGGTTCCACCCACAATTGGCAACACATCACCGACCAAATTGGCATGTTCTGTTTCACAGGGCTAA
AGCCTGAACAGGTGGAGCGGCTGATCAAGGAGTTCTCCATCTACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCCAGCA
ACGTGGGCTACCTTGCCCATGCCATTCACCAGGTCACCAAGTAATGTCCCTGGTGCGAGGAAACAGAGACAACCTTTCTGTCTTCAGCCT
CTGCTATTGAGAGCTTCACACAGACAATGAGAGAGGGTGGATGGTGGTGAGTGGATCATTTCTTTCAGCCACAGTGTGTAACACTCAGCA
TTTGAATGTTTCTCAGAAAAGAACATGTAGTGACACAGGGCAGAGGCATCCATGGCTGGCGTCTGGAATATTAAACCAAACTCTCCCCGG
TCCTTTTTTCTCCAACTTTTCTCAAAGAGTTTACATGTGCAAGAAAGTCATCGCACCAAAAAACCTGTCAATTATGCCATTGCAATATTT
CAGAAGCTTTAACTGAAGTGTCAGGTTCCTCGTGAGAAACAGCACACGTTAGAGGCTTTGAGAGAAGGCCTAGTTCTGTCATGAGTAGTC
GGCCTCGTGTCTGTCCTCCCATCTTGGAACAACCTTATCAACAGGCCGCACTGCAGAAATGATGTTTTATGAAAACCAATGAGGCTGCTG
CCACTCCAGCAAGGGAAATAATGCAGTTTCCTGTCTTATTTAAGAAAAAGAGAAGGCTCTCTTTTCTCCCTTGTCATTGCCGTTCTTTTC
CTTACACGCAAAGATTTTTTAACTATTGCAGATTTTCATCCCATTCTACTGCTTGATTGACCATCAACTCCATCCTATCGAGATTTATTT
AAGAATGAAGAACATAATTTTCTGCTGATGCTGTACCCTCACCCTTTTCAGCAAAGAATAGTGGAGAGTAGGAAACTGTACTTTATCTCG
GCATCCTCTTGAATGATAGTGCAAGTTTCTCCAGTTGGGATGTTGTCTCTGCCCGGTTGGACCTCCTCCCTTTGTTGAATGTGGTGTGCA
GCCTCTCATCTCACACTGTGAGTCCAGCGGCGCAGGGTGGTACCAGGAAAGAGGATATTCTAGGCTTTGCGTGCTGCTAGCTGGGTTCAG
GCTTCACCCACTGGAAAGAACCACCATCTGCTCTAACCATGTAGACTTATTGCGGCCTGGTTTCTCTGTTACAATAAAATTACTGTAGAC
CCAA

>17712_17712_5_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000569240_GOT2_chr16_58757806_ENST00000245206_length(amino acids)=1067AA_BP=667
MLPPQPAMNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLIT
KPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGF
QDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVG
YGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLN
FKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNR
EIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPS
IRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRR
CPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKA
EAQIAAKNLDKEYLPIGGLAEFCKASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAG
MQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRH
FIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVESQLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIG
MRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK

--------------------------------------------------------------
>17712_17712_6_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000569240_GOT2_chr16_58757806_ENST00000434819_length(transcript)=3688nt_BP=2260nt
GCCATTTTGTAGAGAAACAAGCGGAGTTAACCGAAGAGGGGGTCGAGGAGAGCCGGAGTCGGGGACCCAGGAGTTTCCTGTGTCCAGCGC
TGCCGGAGCCGCCTGAGGTGCCATGTTTCAGAACAGAGTAAGACCCCTGGTAAAGAAGAACTGAAGATATTATACAGATACCAGATATAG
CCTAATTACAAAGAAAGCATTAACCTGCCTCTGAGGTGACTAAAGGGGAATAATGGTGATTTTGCGCCGGGCTCGGCCGCCTGCTTCCGC
CCCAACCAGCAATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAAATTACCGAG
CCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTTATTACGCTGCCTATTTTCGCATGTGGATT
TCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAGACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCAAATT
TTATCTCGACGCTGTCCTATGCCATTGATAATCCATTGCACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTA
AAGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTTGGCCTTGCCCTGTTGAATTCTTCCAGCTCAGATCTTAGAGGTTTCGCTGCCC
AGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCTTACATTGACGCAGACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAG
CAATAGAGGTCCTACACCTCCTCCTCTCCCATCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCTTTTC
TTAAGACGCTGCGCAGAGATTTTCCCCAAGAACGCTGTCCCGTGGTGCTCGCACCACTTTTATACCCTGAAAAACGGGACATTCTAATGG
ACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACCATGATGGAGAGCTCTTTGGCTGATTTCATGCAAGAAGTAGGCTATGGCTTTT
GTGCAAGTATTGAAGAATGTCGCAATATAATCGTGCAGTTTGGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGAATGA
TGGCTCGAACTCATTCAGGATTAACAGATGGCATTCCATTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGTGATGGGAAAGATA
AAAGTGATGGAGCACAGGCACACACATGGAATGTAGAAGTCTTGATTGACGTTCTTAAAGAACTGAATCCAAGTTTGAATTTCAAGGAAG
TAACTTATGAACTGGACCATCCTGGATTTCAAATTCGTGACAGTAAAGGACTTCATAATGTGGTTTATGGCATTCAGAGGGGTTTGGGTA
TGGAAGTGTTCCCAGTAGACCTCATATATAGACCTTGGAAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATAAATCCAG
AGATCTTCTGTTTTGCTGACTATCCCTGTCATACTGTTGCCACTGATATTCTGAAAGCACCACCAGAGGATGACAATCGAGAAATTGCCA
CATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGAGCAAGTCAAACAGCTCTTCAGCTTCCCTA
TCAAACACTGTCCAGACATGCTGGTATTGGCCTTACTACAAATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACTCTGA
TGCCAATTTTCCTTGGAAACCATCCTAACTCAGCTATTATTTTGCACTATGCATGGCATGGGCAGGGACAGTCTCCCTCAATTCGCCAAC
TTATCATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATACTTGATGTGGCCCAGGACT
TGAAGGCCTTGTCAATGCTGCTAAATGGTACTCCATTTGCCTTTGTTATTGACCTTGCTGCACTTGCTTCACGTCGTGAATACCTCAAAC
TTGATAAGTGGCTCACAGATAAAATTCGAGAGCATGGGGAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAGAGACGGTGTCCTTCTA
TTTTGGGCGGACTTGCCCCAGAAAAAGACCAGCCCAAAAGTGCTCAACTTCCTCCAGAAACTTTGGCGACAATGTTGGCCTGTCTGCAAG
CTTGTGCAGGCTCCTGGTGGACCCATGTGGAAATGGGACCTCCAGATCCCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACCAATA
GCAAAAAGATGAATCTGGGAGTTGGTGCCTACCGGGATGATAATGGAAAGCCTTACGTTCTGCCTAGCGTCCGCAAGTTTGTCACTGTGC
AGACCATTTCTGGAACTGGAGCCTTAAGGATCGGAGCCAGTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAAC
CAACCTGGGGAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGGTATTATGACCCCAAGACTTGCGGTTTTG
ACTTCACAGGCGCTGTGGAGGATATTTCAAAAATACCAGAGCAGAGTGTTCTTCTTCTGCATGCCTGCGCCCACAATCCCACGGGAGTGG
ACCCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTGGTGAAGAAAAGGAATCTCTTTGCGTTCTTTGACATGGCCTACCAAGGCTTTG
CCAGTGGTGATGGTGATAAGGATGCCTGGGCTGTGCGCCACTTCATCGAACAGGGCATTAATGTTTGCCTCTGCCAATCATATGCCAAGA
ACATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGCAAAGATGCGGATGAAGCCAAAAGGGTAGAGTCACAGTTGAAGA
TCTTGATCCGTCCCATGTATTCCAACCCTCCCCTCAATGGGGCCCGGATTGCTGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAAT
GGCTGCAAGAAGTGAAAGTCATGGCTGACCGCATCATTGGCATGCGGACTCAACTGGTCTCCAACCTCAAGAAGGAGGGTTCCACCCACA
ATTGGCAACACATCACCGACCAAATTGGCATGTTCTGTTTCACAGGGCTAAAGCCTGAACAGGTGGAGCGGCTGATCAAGGAGTTCTCCA
TCTACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCCAGCAACGTGGGCTACCTTGCCCATGCCATTCACCAGGTCACCA
AGTAATGTCCCTGGTGCGAGGAAACAGAGACAACCTTTCTGTCTTCAGCCTCTGCTATTGAGAGCTTCACACAGACAATGAGAGAGGGTG
GATGGTGGTGAGTGGATCATTTCTTTCAGCCACAGTGTGTAACACTCAGCATTTGAATGTTTCTCAGAAAAGAACATGTAGTGACACAGG
GCAGAGGCATCCATGGCTGGCGTCTGGAATATTAAACCAAACTCTCCCCGGTCCTTTTTTCTCCAACTTTTCTCAAAGAGTTTACATGTG
CAAGAAAGTCATCGCACCAAAAAACCTGTCAATTATGCCATTGCAATATTTCAGAAGCTTTAACTGAAGTGTCAGGTTCCTCGTGAGA

>17712_17712_6_CNOT1-GOT2_CNOT1_chr16_58608513_ENST00000569240_GOT2_chr16_58757806_ENST00000434819_length(amino acids)=1024AA_BP=667
MLPPQPAMNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLIT
KPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGF
QDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVG
YGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLN
FKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNR
EIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPS
IRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRR
CPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKF
VTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNP
TGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVES
QLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIK
EFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK

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Fusion Gene PPI Analysis for CNOT1-GOT2

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	CNOT1	chr16:58608513	chr16:58757806	ENST00000317147	-	16	49	1090_1605	659.6666666666666	2377.0	CNOT6%2C CNOT6L%2C CNOT7 and CNOT8
Hgene	CNOT1	chr16:58608513	chr16:58757806	ENST00000441024	-	16	31	1090_1605	659.6666666666666	1552.0	CNOT6%2C CNOT6L%2C CNOT7 and CNOT8
Hgene	CNOT1	chr16:58608513	chr16:58757806	ENST00000569240	-	16	49	1090_1605	659.6666666666666	2372.0	CNOT6%2C CNOT6L%2C CNOT7 and CNOT8
Hgene	CNOT1	chr16:58608513	chr16:58757806	ENST00000317147	-	16	49	800_1015	659.6666666666666	2377.0	ZFP36
Hgene	CNOT1	chr16:58608513	chr16:58757806	ENST00000441024	-	16	31	800_1015	659.6666666666666	1552.0	ZFP36
Hgene	CNOT1	chr16:58608513	chr16:58757806	ENST00000569240	-	16	49	800_1015	659.6666666666666	2372.0	ZFP36

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs for CNOT1-GOT2

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

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Related Diseases for CNOT1-GOT2

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	CNOT1	C0557874	Global developmental delay	1	GENOMICS_ENGLAND
Tgene		C0015544	Failure to Thrive	1	GENOMICS_ENGLAND
Tgene		C0025958	Microcephaly	1	GENOMICS_ENGLAND
Tgene		C0036572	Seizures	1	GENOMICS_ENGLAND
Tgene		C0220994	Hyperammonemia	1	GENOMICS_ENGLAND
Tgene		C0232466	Feeding difficulties	1	GENOMICS_ENGLAND
Tgene		C0557874	Global developmental delay	1	GENOMICS_ENGLAND
Tgene		C1836440	Increased serum lactate	1	GENOMICS_ENGLAND
Tgene		C3714756	Intellectual Disability	1	GENOMICS_ENGLAND
Tgene		C4022810	Abnormality of nervous system morphology	1	GENOMICS_ENGLAND

Fusion Gene Studies in Kim Lab

Fusion gene:CNOT1-GOT2 (FusionGDB2 ID:HG23019TG2806)

Fusion Gene Summary for CNOT1-GOT2

Fusion Gene ORF analysis for CNOT1-GOT2

Fusion Genomic Features for CNOT1-GOT2

Fusion Protein Features for CNOT1-GOT2

Fusion Gene Sequence for CNOT1-GOT2

Fusion Gene PPI Analysis for CNOT1-GOT2

Related Drugs for CNOT1-GOT2

Related Diseases for CNOT1-GOT2

Fusion Gene Studies
in Kim Lab