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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ARHGEF26-NLGN1 (FusionGDB2 ID:HG26084TG22871)

Fusion Gene Summary for ARHGEF26-NLGN1

check button Fusion gene summary
Fusion gene informationFusion gene name: ARHGEF26-NLGN1
Fusion gene ID: hg26084tg22871
HgeneTgene
Gene symbol

ARHGEF26

NLGN1

Gene ID

26084

22871

Gene nameRho guanine nucleotide exchange factor 26neuroligin 1
SynonymsCSGEF|HMFN1864|SGEFNL1
Cytomap('ARHGEF26')('NLGN1')

3q25.2

3q26.31

Type of geneprotein-codingprotein-coding
Descriptionrho guanine nucleotide exchange factor 26Rho guanine nucleotide exchange factor (GEF) 26SH3 domain-containing guanine exchange factorSrc homology 3 domain-containing guanine nucleotide exchange factortesticular tissue protein Li 171neuroligin-1
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000356448, ENST00000465093, 
ENST00000465817, ENST00000483068, 
Fusion gene scores* DoF score6 X 6 X 5=18013 X 8 X 9=936
# samples 615
** MAII scorelog2(6/180*10)=-1.58496250072116
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(15/936*10)=-2.64154602908752
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ARHGEF26 [Title/Abstract] AND NLGN1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointARHGEF26(153870721)-NLGN1(173993105), # samples:1
Anticipated loss of major functional domain due to fusion event.ARHGEF26-NLGN1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneNLGN1

GO:0031175

neuron projection development

22750515

TgeneNLGN1

GO:0051965

positive regulation of synapse assembly

24613359



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4Non-CancerTCGA-BH-A1EW-11BARHGEF26chr3

153870721

+NLGN1chr3

173993105

+


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Fusion Gene ORF analysis for ARHGEF26-NLGN1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-3UTRENST00000356448ENST00000466350ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
5CDS-3UTRENST00000465093ENST00000466350ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
Frame-shiftENST00000356448ENST00000361589ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
Frame-shiftENST00000356448ENST00000401917ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
Frame-shiftENST00000356448ENST00000457714ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
Frame-shiftENST00000356448ENST00000545397ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
Frame-shiftENST00000465093ENST00000361589ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
Frame-shiftENST00000465093ENST00000401917ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
Frame-shiftENST00000465093ENST00000457714ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
Frame-shiftENST00000465093ENST00000545397ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3CDSENST00000465817ENST00000361589ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3CDSENST00000465817ENST00000401917ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3CDSENST00000465817ENST00000457714ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3CDSENST00000465817ENST00000545397ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3CDSENST00000483068ENST00000361589ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3CDSENST00000483068ENST00000401917ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3CDSENST00000483068ENST00000457714ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3CDSENST00000483068ENST00000545397ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3UTRENST00000465817ENST00000466350ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+
intron-3UTRENST00000483068ENST00000466350ARHGEF26chr3

153870721

+NLGN1chr3

173993105

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ARHGEF26-NLGN1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
ARHGEF26chr3153870721+NLGN1chr3173993104+4.86E-060.9999951
ARHGEF26chr3153870721+NLGN1chr3173993104+4.86E-060.9999951


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for ARHGEF26-NLGN1


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:153870721/:173993105)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ARHGEF26-NLGN1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ARHGEF26-NLGN1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ARHGEF26-NLGN1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ARHGEF26-NLGN1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC0004352Autistic Disorder1CTD_human
TgeneC0005586Bipolar Disorder1PSYGENET
TgeneC0036341Schizophrenia1PSYGENET
TgeneC3495676Anorectal Malformations1GENOMICS_ENGLAND