Fusion Gene Studies
in Kim Lab

FusionBase FusionGDB FusionGDB2 FusionPDB FusionNeoAntigen FusionAI FusionNW FGviewer Publication Contact
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact
Center for Computational Systems Medicine
leaf

Fusion Gene Summary

leaf

Fusion Gene ORF analysis

leaf

Fusion Genomic Features

leaf

Fusion Protein Features

leaf

Fusion Gene Sequence

leaf

Fusion Gene PPI analysis

leaf

Related Drugs

leaf

Related Diseases

Fusion gene:ARFIP1-NAA15 (FusionGDB2 ID:HG27236TG80155)

Fusion Gene Summary for ARFIP1-NAA15

check button Fusion gene summary
Fusion gene informationFusion gene name: ARFIP1-NAA15
Fusion gene ID: hg27236tg80155
HgeneTgene
Gene symbol

ARFIP1

NAA15

Gene ID

27236

80155

Gene nameADP ribosylation factor interacting protein 1N-alpha-acetyltransferase 15, NatA auxiliary subunit
SynonymsHSU52521Ga19|MRD50|NARG1|NAT1P|NATH|TBDN|TBDN100
Cytomap('ARFIP1')('NAA15')

4q31.3

4q31.1

Type of geneprotein-codingprotein-coding
Descriptionarfaptin-1N-alpha-acetyltransferase 15, NatA auxiliary subunitN-terminal acetyltransferaseNMDA receptor regulated 1NMDA receptor-regulated protein 1gastric cancer antigen Ga19protein tubedown-1transcriptional coactivator tubedown-100tubedown-1
Modification date2020031320200313
UniProtAcc

P53367

.
Ensembl transtripts involved in fusion geneENST00000353617, ENST00000356064, 
ENST00000405727, ENST00000429148, 
ENST00000451320, ENST00000511289, 
Fusion gene scores* DoF score11 X 7 X 7=53914 X 11 X 8=1232
# samples 1115
** MAII scorelog2(11/539*10)=-2.29278174922785
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(15/1232*10)=-3.03796785019902
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ARFIP1 [Title/Abstract] AND NAA15 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointARFIP1(153701378)-NAA15(140314745), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneARFIP1

GO:0006886

intracellular protein transport

12606037

HgeneARFIP1

GO:0050708

regulation of protein secretion

12606037

TgeneNAA15

GO:0006474

N-terminal protein amino acid acetylation

15496142

TgeneNAA15

GO:0045893

positive regulation of transcription, DNA-templated

12145306



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4STADTCGA-HU-A4GH-01AARFIP1chr4

153701378

+NAA15chr4

140314745

+


Top

Fusion Gene ORF analysis for ARFIP1-NAA15

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5UTR-5UTRENST00000353617ENST00000515576ARFIP1chr4

153701378

+NAA15chr4

140314745

+
5UTR-5UTRENST00000356064ENST00000515576ARFIP1chr4

153701378

+NAA15chr4

140314745

+
5UTR-intronENST00000353617ENST00000296543ARFIP1chr4

153701378

+NAA15chr4

140314745

+
5UTR-intronENST00000353617ENST00000398947ARFIP1chr4

153701378

+NAA15chr4

140314745

+
5UTR-intronENST00000353617ENST00000480277ARFIP1chr4

153701378

+NAA15chr4

140314745

+
5UTR-intronENST00000356064ENST00000296543ARFIP1chr4

153701378

+NAA15chr4

140314745

+
5UTR-intronENST00000356064ENST00000398947ARFIP1chr4

153701378

+NAA15chr4

140314745

+
5UTR-intronENST00000356064ENST00000480277ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-5UTRENST00000405727ENST00000515576ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-5UTRENST00000429148ENST00000515576ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-5UTRENST00000451320ENST00000515576ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-5UTRENST00000511289ENST00000515576ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000405727ENST00000296543ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000405727ENST00000398947ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000405727ENST00000480277ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000429148ENST00000296543ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000429148ENST00000398947ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000429148ENST00000480277ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000451320ENST00000296543ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000451320ENST00000398947ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000451320ENST00000480277ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000511289ENST00000296543ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000511289ENST00000398947ARFIP1chr4

153701378

+NAA15chr4

140314745

+
intron-intronENST00000511289ENST00000480277ARFIP1chr4

153701378

+NAA15chr4

140314745

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

Top

Fusion Genomic Features for ARFIP1-NAA15


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
ARFIP1chr4153701378+NAA15chr4140314744+0.0111879060.9888121
ARFIP1chr4153701378+NAA15chr4140314744+0.0111879060.9888121


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

Top

Fusion Protein Features for ARFIP1-NAA15


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:153701378/:140314745)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
ARFIP1

P53367

.
FUNCTION: Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed:22981988). Mechanisitically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed:9038142, PubMed:22981988). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed:22981988). In turn, ARFs induce fission (PubMed:22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed:22981988). {ECO:0000269|PubMed:22981988, ECO:0000269|PubMed:9038142}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


Top

Fusion Gene Sequence for ARFIP1-NAA15


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

Top

Fusion Gene PPI Analysis for ARFIP1-NAA15


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs for ARFIP1-NAA15


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

Top

Related Diseases for ARFIP1-NAA15


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC0020796Profound Mental Retardation1CTD_human
TgeneC0025363Mental Retardation, Psychosocial1CTD_human
TgeneC0917816Mental deficiency1CTD_human
TgeneC3714756Intellectual Disability1CTD_human;GENOMICS_ENGLAND
TgeneC4540470MENTAL RETARDATION, AUTOSOMAL DOMINANT 501GENOMICS_ENGLAND;UNIPROT