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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:MGMT-SNORA31 (FusionGDB2 ID:HG4255TG677814)

Fusion Gene Summary for MGMT-SNORA31

check button Fusion gene summary
Fusion gene informationFusion gene name: MGMT-SNORA31
Fusion gene ID: hg4255tg677814
HgeneTgene
Gene symbol

MGMT

SNORA31

Gene ID

4255

677814

Gene nameO-6-methylguanine-DNA methyltransferasesmall nucleolar RNA, H/ACA box 31
Synonyms-ACA31|SNORA31A
Cytomap('MGMT')('SNORA31')

10q26.3

13q14.13

Type of geneprotein-codingsnoRNA
Descriptionmethylated-DNA--protein-cysteine methyltransferase6-O-methylguanine-DNA methyltransferaseO-6-methylguanine-DNA-alkyltransferaseO6-methylguanine-DNA methyltransferasemethylguanine-DNA methyltransferaseACA31 snoRNA
Modification date2020031520200313
UniProtAcc

P16455

.
Ensembl transtripts involved in fusion geneENST00000306010, ENST00000462672, 
Fusion gene scores* DoF score10 X 7 X 6=4202 X 1 X 2=4
# samples 112
** MAII scorelog2(11/420*10)=-1.93288580414146
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(2/4*10)=2.32192809488736
Context

PubMed: MGMT [Title/Abstract] AND SNORA31 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointMGMT(131265560)-SNORA31(42063681), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneMGMT

GO:0043066

negative regulation of apoptotic process

24147153

HgeneMGMT

GO:2000781

positive regulation of double-strand break repair

24147153



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LIHCTCGA-XR-A8TF-01AMGMTchr10

131265560

+SNORA31chr14

42063681

+


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Fusion Gene ORF analysis for MGMT-SNORA31

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-3UTRENST00000306010ENST00000516049MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000362607MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000515993MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516019MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516029MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516190MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516241MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516287MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516327MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516429MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516482MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516528MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516540MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516624MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516728MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516771MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516773MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000516953MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517043MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517079MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517111MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517180MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517204MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517219MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517232MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517242MGMTchr10

131265560

+SNORA31chr14

42063681

+
5CDS-intronENST00000306010ENST00000517250MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-3UTRENST00000462672ENST00000516049MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000362607MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000515993MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516019MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516029MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516190MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516241MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516287MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516327MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516429MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516482MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516528MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516540MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516624MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516728MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516771MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516773MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000516953MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517043MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517079MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517111MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517180MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517204MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517219MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517232MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517242MGMTchr10

131265560

+SNORA31chr14

42063681

+
intron-intronENST00000462672ENST00000517250MGMTchr10

131265560

+SNORA31chr14

42063681

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for MGMT-SNORA31


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


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Fusion Protein Features for MGMT-SNORA31


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:131265560/:42063681)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
MGMT

P16455

.
FUNCTION: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for MGMT-SNORA31


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for MGMT-SNORA31


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for MGMT-SNORA31


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status
HgeneMGMTP16455DB14548Zinc sulfate, unspecified formStabilizationSmall moleculeApproved|Experimental
HgeneMGMTP16455DB01593ZincSmall moleculeApproved|Investigational
HgeneMGMTP16455DB14487Zinc acetateSmall moleculeApproved|Investigational
HgeneMGMTP16455DB14533Zinc chlorideStabilizationSmall moleculeApproved|Investigational
HgeneMGMTP16455DB00151CysteineSmall moleculeApproved|Nutraceutical

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Related Diseases for MGMT-SNORA31


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneMGMTC0334588Giant Cell Glioblastoma4CTD_human;ORPHANET
HgeneMGMTC0017638Glioma3CTD_human
HgeneMGMTC0259783mixed gliomas3CTD_human
HgeneMGMTC0555198Malignant Glioma3CTD_human
HgeneMGMTC0017636Glioblastoma2CTD_human
HgeneMGMTC0206726gliosarcoma2ORPHANET
HgeneMGMTC1621958Glioblastoma Multiforme2CTD_human
HgeneMGMTC0006118Brain Neoplasms1CTD_human
HgeneMGMTC0023418leukemia1CTD_human
HgeneMGMTC0023893Liver Cirrhosis, Experimental1CTD_human
HgeneMGMTC0026640Mouth Neoplasms1CTD_human
HgeneMGMTC0027540Necrosis1CTD_human
HgeneMGMTC0032927Precancerous Conditions1CTD_human
HgeneMGMTC0153381Malignant neoplasm of mouth1CTD_human
HgeneMGMTC0153633Malignant neoplasm of brain1CTD_human
HgeneMGMTC0279626Squamous cell carcinoma of esophagus1CTD_human
HgeneMGMTC0282313Condition, Preneoplastic1CTD_human
HgeneMGMTC0496899Benign neoplasm of brain, unspecified1CTD_human
HgeneMGMTC0750974Brain Tumor, Primary1CTD_human
HgeneMGMTC0750977Recurrent Brain Neoplasm1CTD_human
HgeneMGMTC0750979Primary malignant neoplasm of brain1CTD_human
HgeneMGMTC1168401Squamous cell carcinoma of the head and neck1CTD_human
HgeneMGMTC1449861Micronuclei, Chromosome-Defective1CTD_human
HgeneMGMTC1449862Micronuclei, Genotoxicant-Induced1CTD_human
HgeneMGMTC1527390Neoplasms, Intracranial1CTD_human
HgeneMGMTC2314896Familial Atypical Mole Melanoma Syndrome1ORPHANET
TgeneC0276226Herpes encephalitis1GENOMICS_ENGLAND