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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:NR2F1-AS1-CYP2A6 (FusionGDB2 ID:HG441094TG1548)

Fusion Gene Summary for NR2F1-AS1-CYP2A6

check button Fusion gene summary
Fusion gene informationFusion gene name: NR2F1-AS1-CYP2A6
Fusion gene ID: hg441094tg1548
HgeneTgene
Gene symbol

NR2F1-AS1

CYP2A6

Gene ID

441094

1548

Gene nameNR2F1 antisense RNA 1cytochrome P450 family 2 subfamily A member 6
Synonyms-CPA6|CYP2A|CYP2A3|CYPIIA6|P450C2A|P450PB
Cytomap('NR2F1-AS1')('CYP2A6')

5q15

19q13.2

Type of genencRNAprotein-coding
Description-cytochrome P450 2A61,4-cineole 2-exo-monooxygenasecoumarin 7-hydroxylasecytochrome P450 IIA3cytochrome P450(I)cytochrome P450, family 2, subfamily A, polypeptide 6cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6flavoprotein-l
Modification date2020032920200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000504474, ENST00000507963, 
ENST00000510254, ENST00000513055, 
ENST00000606165, ENST00000606188, 
ENST00000606233, ENST00000606696, 
ENST00000606739, ENST00000607112, 
ENST00000607195, ENST00000607797, 
ENST00000607831, 
Fusion gene scores* DoF score5 X 5 X 1=255 X 6 X 3=90
# samples 55
** MAII scorelog2(5/25*10)=1
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0		log2(5/90*10)=-0.84799690655495
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: NR2F1-AS1 [Title/Abstract] AND CYP2A6 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointNR2F1-AS1(92917802)-CYP2A6(41350693), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneCYP2A6

GO:0009804

coumarin metabolic process

19651758

TgeneCYP2A6

GO:0017144

drug metabolic process

19651758

TgeneCYP2A6

GO:0042738

exogenous drug catabolic process

15680923

TgeneCYP2A6

GO:0046226

coumarin catabolic process

19029318



check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand


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Fusion Gene ORF analysis for NR2F1-AS1-CYP2A6

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for NR2F1-AS1-CYP2A6


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


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Fusion Protein Features for NR2F1-AS1-CYP2A6


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:92917802/:41350693)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for NR2F1-AS1-CYP2A6


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for NR2F1-AS1-CYP2A6


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for NR2F1-AS1-CYP2A6


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for NR2F1-AS1-CYP2A6


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC0024121Lung Neoplasms6CTD_human
TgeneC0242379Malignant neoplasm of lung6CTD_human
TgeneC0028043Nicotine Dependence5CTD_human
TgeneC0040332Tobacco Dependence5CTD_human
TgeneC0376384Nicotine Use Disorder5CTD_human
TgeneC0024623Malignant neoplasm of stomach2CTD_human
TgeneC0038356Stomach Neoplasms2CTD_human
TgeneC1708349Hereditary Diffuse Gastric Cancer2CTD_human
TgeneC1861063TOBACCO ADDICTION, SUSCEPTIBILITY TO (finding)2CTD_human
TgeneC0001418Adenocarcinoma1CTD_human
TgeneC0006111Brain Diseases1CTD_human
TgeneC0009402Colorectal Carcinoma1CTD_human
TgeneC0009404Colorectal Neoplasms1CTD_human
TgeneC0019163Hepatitis B1CTD_human
TgeneC0019193Hepatitis, Toxic1CTD_human
TgeneC0019196Hepatitis C1CTD_human
TgeneC0023897Liver Diseases, Parasitic1CTD_human
TgeneC0027439Nasopharyngeal Neoplasms1CTD_human
TgeneC0029106Opisthorchiasis1CTD_human
TgeneC0033578Prostatic Neoplasms1CTD_human
TgeneC0034067Pulmonary Emphysema1CTD_human
TgeneC0085584Encephalopathies1CTD_human
TgeneC0205641Adenocarcinoma, Basal Cell1CTD_human
TgeneC0205642Adenocarcinoma, Oxyphilic1CTD_human
TgeneC0205643Carcinoma, Cribriform1CTD_human
TgeneC0205644Carcinoma, Granular Cell1CTD_human
TgeneC0205645Adenocarcinoma, Tubular1CTD_human
TgeneC0206704Carcinoma, Large Cell1CTD_human
TgeneC0221227Centriacinar Emphysema1CTD_human
TgeneC0235874Disease Exacerbation1CTD_human
TgeneC0238301Cancer of Nasopharynx1CTD_human
TgeneC0262584Carcinoma, Small Cell1CTD_human
TgeneC0264393Panacinar Emphysema1CTD_human
TgeneC0277004Opisthorchis felineus Infection1CTD_human
TgeneC0277005Opisthorchis viverrini Infection1CTD_human
TgeneC0279626Squamous cell carcinoma of esophagus1CTD_human
TgeneC0376358Malignant neoplasm of prostate1CTD_human
TgeneC0860207Drug-Induced Liver Disease1CTD_human
TgeneC1262760Hepatitis, Drug-Induced1CTD_human
TgeneC2350878Focal Emphysema1CTD_human
TgeneC3658290Drug-Induced Acute Liver Injury1CTD_human
TgeneC4277682Chemical and Drug Induced Liver Injury1CTD_human
TgeneC4279912Chemically-Induced Liver Toxicity1CTD_human