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Fusion Gene Summary | |
Fusion Gene ORF analysis | |
Fusion Genomic Features | |
Fusion Protein Features | |
Fusion Gene Sequence | |
Fusion Gene PPI analysis | |
Related Drugs | |
Related Diseases |
Fusion gene:FBXL8-DDB2 (FusionGDB2 ID:HG55336TG1643) |
Fusion Gene Summary for FBXL8-DDB2 |
Fusion gene summary |
Fusion gene information | Fusion gene name: FBXL8-DDB2 | Fusion gene ID: hg55336tg1643 | Hgene | Tgene | Gene symbol | FBXL8 | DDB2 | Gene ID | 55336 | 1643 |
Gene name | F-box and leucine rich repeat protein 8 | damage specific DNA binding protein 2 | |
Synonyms | FBL8 | DDBB|UV-DDB2|XPE | |
Cytomap | ('FBXL8')('DDB2') 16q22.1 | 11p11.2 | |
Type of gene | protein-coding | protein-coding | |
Description | F-box/LRR-repeat protein 8F-box protein FBL8 | DNA damage-binding protein 2DDB p48 subunitUV-DDB 2UV-damaged DNA-binding protein 2damage-specific DNA binding protein 2, 48kDaxeroderma pigmentosum group E protein | |
Modification date | 20200313 | 20200327 | |
UniProtAcc | . | Q92466 | |
Ensembl transtripts involved in fusion gene | ENST00000258200, ENST00000518148, ENST00000519917, ENST00000521920, | ||
Fusion gene scores | * DoF score | 7 X 2 X 3=42 | 12 X 6 X 7=504 |
# samples | 6 | 11 | |
** MAII score | log2(6/42*10)=0.514573172829758 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | log2(11/504*10)=-2.19592020997526 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Context | PubMed: FBXL8 [Title/Abstract] AND DDB2 [Title/Abstract] AND fusion [Title/Abstract] | ||
Most frequent breakpoint | FBXL8(67195840)-DDB2(47259387), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. | FBXL8-DDB2 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. FBXL8-DDB2 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. FBXL8-DDB2 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
Partner | Gene | GO ID | GO term | PubMed ID |
Tgene | DDB2 | GO:0000209 | protein polyubiquitination | 12732143 |
Tgene | DDB2 | GO:0009411 | response to UV | 12732143 |
Tgene | DDB2 | GO:0035518 | histone H2A monoubiquitination | 22334663 |
Tgene | DDB2 | GO:0051865 | protein autoubiquitination | 12732143 |
Tgene | DDB2 | GO:0070914 | UV-damage excision repair | 22334663 |
Fusion gene information * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
ChimerDB4 | STAD | TCGA-CG-5725 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
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Fusion Gene ORF analysis for FBXL8-DDB2 |
Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
5CDS-3UTR | ENST00000258200 | ENST00000378601 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
5CDS-3UTR | ENST00000518148 | ENST00000378601 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
5CDS-3UTR | ENST00000519917 | ENST00000378601 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
5CDS-3UTR | ENST00000521920 | ENST00000378601 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000258200 | ENST00000256996 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000258200 | ENST00000378600 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000258200 | ENST00000378603 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000518148 | ENST00000256996 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000518148 | ENST00000378600 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000518148 | ENST00000378603 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000519917 | ENST00000256996 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000519917 | ENST00000378600 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000519917 | ENST00000378603 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000521920 | ENST00000256996 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000521920 | ENST00000378600 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
Frame-shift | ENST00000521920 | ENST00000378603 | FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + |
ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for FBXL8-DDB2 |
FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + | 0.1154893 | 0.88451064 |
FBXL8 | chr16 | 67195840 | + | DDB2 | chr11 | 47259387 | + | 0.1154893 | 0.88451064 |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for FBXL8-DDB2 |
Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:67195840/:47259387) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
Main function of each fusion partner protein. (from UniProt) |
Hgene | Tgene |
. | DDB2 |
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}. | FUNCTION: Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:9892649, PubMed:12732143, PubMed:15882621, PubMed:16473935, PubMed:18593899). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:16260596, PubMed:12944386, PubMed:14751237). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:16260596, PubMed:12944386). Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1) (PubMed:12732143, PubMed:15882621, PubMed:16473935, PubMed:18593899, PubMed:26572825). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PubMed:16678110, PubMed:16473935). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed:16678110, PubMed:16473935). The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed:15882621). The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR (PubMed:26572825). {ECO:0000269|PubMed:10882109, ECO:0000269|PubMed:11278856, ECO:0000269|PubMed:11705987, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:12944386, ECO:0000269|PubMed:14751237, ECO:0000269|PubMed:15882621, ECO:0000269|PubMed:16260596, ECO:0000269|PubMed:16473935, ECO:0000269|PubMed:16678110, ECO:0000269|PubMed:18593899, ECO:0000269|PubMed:26572825, ECO:0000269|PubMed:9892649}.; FUNCTION: [Isoform D1]: Inhibits UV-damaged DNA repair. {ECO:0000269|PubMed:14751237}.; FUNCTION: [Isoform D2]: Inhibits UV-damaged DNA repair. {ECO:0000269|PubMed:14751237}. |
Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
- In-frame and retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
- In-frame and not-retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for FBXL8-DDB2 |
For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for FBXL8-DDB2 |
Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
Hgene | Hgene's interactors | Tgene | Tgene's interactors |
- Retained PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
- Lost PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
- Retained PPIs, but lost function due to frame-shift fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for FBXL8-DDB2 |
Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for FBXL8-DDB2 |
Diseases associated with fusion partners. (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
Tgene | C1848411 | XERODERMA PIGMENTOSUM, COMPLEMENTATION GROUP E | 9 | CLINGEN;CTD_human;GENOMICS_ENGLAND;UNIPROT |