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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:NPLOC4-CYP4B1 (FusionGDB2 ID:HG55666TG1580)

Fusion Gene Summary for NPLOC4-CYP4B1

check button Fusion gene summary
Fusion gene informationFusion gene name: NPLOC4-CYP4B1
Fusion gene ID: hg55666tg1580
HgeneTgene
Gene symbol

NPLOC4

CYP4B1

Gene ID

55666

1580

Gene nameNPL4 homolog, ubiquitin recognition factorcytochrome P450 family 4 subfamily B member 1
SynonymsNPL4CYPIVB1|P-450HP
Cytomap('NPLOC4')('CYP4B1')

17q25.3

1p33

Type of geneprotein-codingprotein-coding
Descriptionnuclear protein localization protein 4 homologNPLOC4 ubiquitin recognition factornuclear protein localization 4 homologcytochrome P450 4B1cytochrome P450, family 4, subfamily B, polypeptide 1cytochrome P450, subfamily IVB, polypeptide 1cytochrome P450-HPmicrosomal monooxygenase
Modification date2020031320200313
UniProtAcc.

P13584

Ensembl transtripts involved in fusion geneENST00000331134, ENST00000374747, 
ENST00000539314, ENST00000574344, 
ENST00000572760, ENST00000573876, 
Fusion gene scores* DoF score19 X 17 X 11=35533 X 3 X 2=18
# samples 303
** MAII scorelog2(30/3553*10)=-3.56600328283534
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(3/18*10)=0.736965594166206
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: NPLOC4 [Title/Abstract] AND CYP4B1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointNPLOC4(79580344)-CYP4B1(47230650), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LIHCTCGA-BC-A3KG-01ANPLOC4chr17

79580344

-CYP4B1chr1

47230650

+


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Fusion Gene ORF analysis for NPLOC4-CYP4B1

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-3UTRENST00000331134ENST00000546128NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-3UTRENST00000374747ENST00000546128NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-intronENST00000331134ENST00000271153NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-intronENST00000331134ENST00000371919NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-intronENST00000331134ENST00000371923NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-intronENST00000331134ENST00000452782NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-intronENST00000374747ENST00000271153NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-intronENST00000374747ENST00000371919NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-intronENST00000374747ENST00000371923NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5CDS-intronENST00000374747ENST00000452782NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-3UTRENST00000539314ENST00000546128NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-3UTRENST00000574344ENST00000546128NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-intronENST00000539314ENST00000271153NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-intronENST00000539314ENST00000371919NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-intronENST00000539314ENST00000371923NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-intronENST00000539314ENST00000452782NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-intronENST00000574344ENST00000271153NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-intronENST00000574344ENST00000371919NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-intronENST00000574344ENST00000371923NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
5UTR-intronENST00000574344ENST00000452782NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-3UTRENST00000572760ENST00000546128NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-3UTRENST00000573876ENST00000546128NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-intronENST00000572760ENST00000271153NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-intronENST00000572760ENST00000371919NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-intronENST00000572760ENST00000371923NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-intronENST00000572760ENST00000452782NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-intronENST00000573876ENST00000271153NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-intronENST00000573876ENST00000371919NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-intronENST00000573876ENST00000371923NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+
intron-intronENST00000573876ENST00000452782NPLOC4chr17

79580344

-CYP4B1chr1

47230650

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for NPLOC4-CYP4B1


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
NPLOC4chr1779580343-CYP4B1chr147230649+9.00E-070.99999905
NPLOC4chr1779580343-CYP4B1chr147230649+9.00E-070.99999905


check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for NPLOC4-CYP4B1


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:79580344/:47230650)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.CYP4B1

P13584

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for NPLOC4-CYP4B1


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for NPLOC4-CYP4B1


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for NPLOC4-CYP4B1


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for NPLOC4-CYP4B1


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneC0005684Malignant neoplasm of urinary bladder1CTD_human
TgeneC0005695Bladder Neoplasm1CTD_human