Fusion Gene Studies
in Kim Lab

FusionBase FusionGDB FusionGDB2 FusionPDB FusionNeoAntigen FusionAI FusionNW FGviewer Publication Contact
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Center for Computational Systems Medicine
leaf

Fusion Gene Summary

leaf

Fusion Gene ORF analysis

leaf

Fusion Genomic Features

leaf

Fusion Protein Features

leaf

Fusion Gene Sequence

leaf

Fusion Gene PPI analysis

leaf

Related Drugs

leaf

Related Diseases

Fusion gene:BIRC6-SPAST (FusionGDB2 ID:HG57448TG6683)

Fusion Gene Summary for BIRC6-SPAST

check button Fusion gene summary
Fusion gene informationFusion gene name: BIRC6-SPAST
Fusion gene ID: hg57448tg6683
HgeneTgene
Gene symbol

BIRC6

SPAST

Gene ID

57448

6683

Gene namebaculoviral IAP repeat containing 6spastin
SynonymsAPOLLON|BRUCEADPSP|FSP2|SPG4
Cytomap('BIRC6')('SPAST')

2p22.3

2p22.3

Type of geneprotein-codingprotein-coding
Descriptionbaculoviral IAP repeat-containing protein 6BIR repeat-containing ubiquitin-conjugating enzymeRING-type E3 ubiquitin transferase BIRC6ubiquitin-conjugating BIR-domain enzyme apollonspastinspastic paraplegia 4 (autosomal dominant; spastin)spastic paraplegia 4 protein
Modification date2020031320200322
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000421745, 
Fusion gene scores* DoF score21 X 23 X 16=77287 X 9 X 7=441
# samples 3310
** MAII scorelog2(33/7728*10)=-4.54955716458996
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(10/441*10)=-2.1407786557828
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: BIRC6 [Title/Abstract] AND SPAST [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointBIRC6(32774548)-SPAST(32372287), # samples:2
Anticipated loss of major functional domain due to fusion event.BIRC6-SPAST seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
BIRC6-SPAST seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
BIRC6-SPAST seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
BIRC6-SPAST seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneSPAST

GO:0001578

microtubule bundle formation

16219033

TgeneSPAST

GO:0034214

protein hexamerization

17389232

TgeneSPAST

GO:0051013

microtubule severing

15716377|16219033|17389232|23745751|26875866

TgeneSPAST

GO:0051260

protein homooligomerization

17389232


check buttonFusion gene breakpoints across BIRC6 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure
check buttonFusion gene breakpoints across SPAST (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4HNSCTCGA-F7-A624-01ABIRC6chr2

32774548

-SPASTchr2

32372287

+
ChimerDB4HNSCTCGA-F7-A624BIRC6chr2

32774548

+SPASTchr2

32372287

+
ChimerDB4SKCMTCGA-ER-A3EV-06ABIRC6chr2

32658872

-SPASTchr2

32323865

+
ChimerDB4TGCTTCGA-2G-AAGA-01ABIRC6chr2

32631625

-SPASTchr2

32339707

+


Top

Fusion Gene ORF analysis for BIRC6-SPAST

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000421745ENST00000315285BIRC6chr2

32658872

-SPASTchr2

32323865

+
5CDS-intronENST00000421745ENST00000345662BIRC6chr2

32658872

-SPASTchr2

32323865

+
In-frameENST00000421745ENST00000315285BIRC6chr2

32774548

+SPASTchr2

32372287

+
In-frameENST00000421745ENST00000315285BIRC6chr2

32631625

-SPASTchr2

32339707

+
In-frameENST00000421745ENST00000345662BIRC6chr2

32774548

+SPASTchr2

32372287

+
In-frameENST00000421745ENST00000345662BIRC6chr2

32631625

-SPASTchr2

32339707

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000421745BIRC6chr232774548+ENST00000345662SPASTchr232372287+1658213278134134414435
ENST00000421745BIRC6chr232774548+ENST00000315285SPASTchr232372287+1658313278134134414435
ENST00000421745BIRC6chr232631625-ENST00000345662SPASTchr232339707+592016111342779881
ENST00000421745BIRC6chr232631625-ENST00000315285SPASTchr232339707+592116111342779881

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000421745ENST00000345662BIRC6chr232774548+SPASTchr232372287+0.000632030.999368
ENST00000421745ENST00000315285BIRC6chr232774548+SPASTchr232372287+0.0006301040.9993699
ENST00000421745ENST00000345662BIRC6chr232631625-SPASTchr232339707+0.0001573860.99984264
ENST00000421745ENST00000315285BIRC6chr232631625-SPASTchr232339707+0.0001569580.999843

Top

Fusion Genomic Features for BIRC6-SPAST


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
BIRC6chr232774548+SPASTchr232372286+1.54E-070.9999999
BIRC6chr232774548+SPASTchr232372286+1.54E-070.9999999

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

Top

Fusion Protein Features for BIRC6-SPAST


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:32774548/chr2:32372287)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneBIRC6chr2:32631625chr2:32339707ENST00000421745-97430_364924858.0Compositional biasNote=Poly-Ala
HgeneBIRC6chr2:32774548chr2:32372287ENST00000421745+65741660_166843814858.0Compositional biasNote=Poly-Ala
HgeneBIRC6chr2:32774548chr2:32372287ENST00000421745+657430_3643814858.0Compositional biasNote=Poly-Ala
HgeneBIRC6chr2:32631625chr2:32339707ENST00000421745-974284_3584924858.0RepeatNote=BIR
HgeneBIRC6chr2:32774548chr2:32372287ENST00000421745+6574284_35843814858.0RepeatNote=BIR
TgeneSPASTchr2:32631625chr2:32339707ENST00000315285317309_312227617.0MotifNuclear localization signal
TgeneSPASTchr2:32631625chr2:32339707ENST00000345662216309_312195585.0MotifNuclear localization signal
TgeneSPASTchr2:32631625chr2:32339707ENST00000315285317382_389227617.0Nucleotide bindingATP
TgeneSPASTchr2:32631625chr2:32339707ENST00000345662216382_389195585.0Nucleotide bindingATP
TgeneSPASTchr2:32631625chr2:32339707ENST00000315285317228_616227617.0RegionSufficient for microtubule severing
TgeneSPASTchr2:32631625chr2:32339707ENST00000345662216228_616195585.0RegionSufficient for microtubule severing

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneBIRC6chr2:32631625chr2:32339707ENST00000421745-9741660_16684924858.0Compositional biasNote=Poly-Ala
HgeneBIRC6chr2:32631625chr2:32339707ENST00000421745-9744573_47404924858.0DomainUBC core
HgeneBIRC6chr2:32774548chr2:32372287ENST00000421745+65744573_474043814858.0DomainUBC core
TgeneSPASTchr2:32631625chr2:32339707ENST00000315285317120_195227617.0DomainMIT
TgeneSPASTchr2:32631625chr2:32339707ENST00000345662216120_195195585.0DomainMIT
TgeneSPASTchr2:32774548chr2:32372287ENST000003152851417120_195562617.0DomainMIT
TgeneSPASTchr2:32774548chr2:32372287ENST000003456621316120_195530585.0DomainMIT
TgeneSPASTchr2:32631625chr2:32339707ENST0000031528531757_77227617.0IntramembraneHelical
TgeneSPASTchr2:32631625chr2:32339707ENST0000034566221657_77195585.0IntramembraneHelical
TgeneSPASTchr2:32774548chr2:32372287ENST00000315285141757_77562617.0IntramembraneHelical
TgeneSPASTchr2:32774548chr2:32372287ENST00000345662131657_77530585.0IntramembraneHelical
TgeneSPASTchr2:32631625chr2:32339707ENST000003152853174_11227617.0MotifNuclear localization signal
TgeneSPASTchr2:32631625chr2:32339707ENST0000031528531759_67227617.0MotifNuclear export signal
TgeneSPASTchr2:32631625chr2:32339707ENST000003456622164_11195585.0MotifNuclear localization signal
TgeneSPASTchr2:32631625chr2:32339707ENST0000034566221659_67195585.0MotifNuclear export signal
TgeneSPASTchr2:32774548chr2:32372287ENST000003152851417309_312562617.0MotifNuclear localization signal
TgeneSPASTchr2:32774548chr2:32372287ENST0000031528514174_11562617.0MotifNuclear localization signal
TgeneSPASTchr2:32774548chr2:32372287ENST00000315285141759_67562617.0MotifNuclear export signal
TgeneSPASTchr2:32774548chr2:32372287ENST000003456621316309_312530585.0MotifNuclear localization signal
TgeneSPASTchr2:32774548chr2:32372287ENST0000034566213164_11530585.0MotifNuclear localization signal
TgeneSPASTchr2:32774548chr2:32372287ENST00000345662131659_67530585.0MotifNuclear export signal
TgeneSPASTchr2:32774548chr2:32372287ENST000003152851417382_389562617.0Nucleotide bindingATP
TgeneSPASTchr2:32774548chr2:32372287ENST000003456621316382_389530585.0Nucleotide bindingATP
TgeneSPASTchr2:32631625chr2:32339707ENST000003152853171_194227617.0RegionRequired for midbody localization
TgeneSPASTchr2:32631625chr2:32339707ENST000003152853171_50227617.0RegionRequired for nuclear localization
TgeneSPASTchr2:32631625chr2:32339707ENST000003456622161_194195585.0RegionRequired for midbody localization
TgeneSPASTchr2:32631625chr2:32339707ENST000003456622161_50195585.0RegionRequired for nuclear localization
TgeneSPASTchr2:32774548chr2:32372287ENST0000031528514171_194562617.0RegionRequired for midbody localization
TgeneSPASTchr2:32774548chr2:32372287ENST0000031528514171_50562617.0RegionRequired for nuclear localization
TgeneSPASTchr2:32774548chr2:32372287ENST000003152851417228_616562617.0RegionSufficient for microtubule severing
TgeneSPASTchr2:32774548chr2:32372287ENST0000034566213161_194530585.0RegionRequired for midbody localization
TgeneSPASTchr2:32774548chr2:32372287ENST0000034566213161_50530585.0RegionRequired for nuclear localization
TgeneSPASTchr2:32774548chr2:32372287ENST000003456621316228_616530585.0RegionSufficient for microtubule severing
TgeneSPASTchr2:32631625chr2:32339707ENST000003152853171_56227617.0Topological domainCytoplasmic
TgeneSPASTchr2:32631625chr2:32339707ENST0000031528531778_616227617.0Topological domainCytoplasmic
TgeneSPASTchr2:32631625chr2:32339707ENST000003456622161_56195585.0Topological domainCytoplasmic
TgeneSPASTchr2:32631625chr2:32339707ENST0000034566221678_616195585.0Topological domainCytoplasmic
TgeneSPASTchr2:32774548chr2:32372287ENST0000031528514171_56562617.0Topological domainCytoplasmic
TgeneSPASTchr2:32774548chr2:32372287ENST00000315285141778_616562617.0Topological domainCytoplasmic
TgeneSPASTchr2:32774548chr2:32372287ENST0000034566213161_56530585.0Topological domainCytoplasmic
TgeneSPASTchr2:32774548chr2:32372287ENST00000345662131678_616530585.0Topological domainCytoplasmic


Top

Fusion Gene Sequence for BIRC6-SPAST


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>9768_9768_1_BIRC6-SPAST_BIRC6_chr2_32631625_ENST00000421745_SPAST_chr2_32339707_ENST00000315285_length(transcript)=5921nt_BP=1611nt
TCCCTGCTTCTCCCCCTCTCCCGTCAGCCTCCCTCCGAGTTTGGCCCCTCCGGCCGGGCGATCGACGTTCCGCGTGCGTGCGGGCGCCTG
ACTTCACTTCCGGCTAACGCGCTCGGCTTGCCCCCTGGCCCCGGATGGTGACTGGTGGTGGTGCTGCACCTCCCGGGACTGTCACTGAGC
CGCTTCCCAGTGTGATTGTGCTGAGCGCAGGCCGGAAGATGGCGGCTGCGGCTGCGGCGGCCTCGGGCCCCGGCTGCTCCTCGGCGGCGG
GGGCGGGGGCGGCCGGGGTCTCAGAGTGGCTGGTGCTGCGGGACGGCTGCATGCACTGCGACGCCGACGGGCTGCACAGCCTGTCCTACC
ACCCTGCGCTCAACGCCATCCTGGCCGTCACTAGCCGCGGGACCATCAAAGTCATCGACGGCACCTCGGGGGCCACACTGCAGGCCTCCG
CGCTCAGTGCTAAACCAGGTGGACAGGTGAAATGTCAGTATATCTCTGCTGTGGATAAAGTTATATTTGTGGATGATTATGCAGTAGGGT
GTAGGAAGGACCTTAATGGAATCTTGTTGTTAGACACTGCTCTGCAAACTCCAGTTTCAAAGCAGGATGATGTGGTTCAGCTTGAATTAC
CCGTTACAGAGGCACAGCAGCTCTTATCAGCATGTTTAGAAAAGGTAGATATTTCTAGTACAGAGGGTTATGATTTGTTCATCACACAGC
TCAAAGATGGTTTAAAAAATACATCTCATGAGACTGCAGCAAACCACAAAGTTGCTAAGTGGGCCACAGTTACATTTCATCTTCCTCATC
ATGTGTTGAAGTCCATTGCCAGTGCCATTGTAAATGAACTCAAGAAAATAAATCAAAATGTTGCTGCCTTACCTGTGGCGTCCTCAGTGA
TGGACAGATTGTCTTACCTCTTACCTAGTGCACGTCCAGAACTCGGAGTGGGGCCAGGCCGTTCTGTAGACAGATCACTGATGTATAGTG
AAGCTAACAGACGGGAGACATTTACCTCATGGCCTCATGTAGGCTATAGGTGGGCACAACCAGATCCCATGGCTCAAGCTGGATTTTATC
ATCAGCCTGCCTCATCTGGAGATGATAGAGCCATGTGTTTTACTTGTAGTGTATGCCTCGTTTGTTGGGAACCTACTGATGAACCTTGGT
CTGAACACGAAAGACATTCCCCAAACTGCCCATTTGTGAAAGGTGAGCACACACAGAATGTGCCATTGTCAGTCACTCTTGCAACAAGTC
CTGCACAGTTTCCTTGTACGGATGGAACTGACAGAATATCTTGCTTTGGGTCGGGGAGCTGCCCTCATTTTCTAGCTGCTGCAACTAAAC
GAGGAAAGATCTGCATATGGGATGTTTCCAAACTTATGAAGGTGCACTTAAAGTTTGAAATTAATGCCTATGATCCAGCAATTGTACAAC
AGCTTATTCTATCAGGAGACCCAAGCTCAGGAGTTGATTCAAGGAGACCAACTTTGGCGTGGCTGGAGGACTCCTCTAGTTGCTCAGATA
TACCAAAATTGGAAGGAGATAGTGATGATTTACTGGAGGATTCAGACAGTGAAGAGCATTCCAGATCAGATTCTGTGACAGAAAGTGGAG
CTGTTCCAAAAAGAAAAGACCCCTTAACACACACTAGTAATTCACTGCCTCGTTCAAAAACAGTTATGAAAACTGGATCTGCAGGCCTTT
CAGGCCACCATAGAGCACCTAGTTACAGTGGTTTATCCATGGTTTCTGGAGTGAAACAGGGATCTGGTCCTGCTCCTACCACTCATAAGG
GTACTCCGAAAACAAATAGGACAAATAAACCTTCTACCCCTACAACTGCTACTCGTAAGAAAAAAGACTTGAAGAATTTTAGGAATGTGG
ACAGCAACCTTGCTAACCTTATAATGAATGAAATTGTGGACAATGGAACAGCTGTTAAATTTGATGATATAGCTGGTCAAGACTTGGCAA
AACAAGCATTGCAAGAAATTGTTATTCTTCCTTCTCTGAGGCCTGAGTTGTTCACAGGGCTTAGAGCTCCTGCCAGAGGGCTGTTACTCT
TTGGTCCACCTGGGAATGGGAAGACAATGCTGGCTAAAGCAGTAGCTGCAGAATCGAATGCAACCTTCTTTAATATAAGTGCTGCAAGTT
TAACTTCAAAATACGTGGGAGAAGGAGAGAAATTGGTGAGGGCTCTTTTTGCTGTGGCTCGAGAACTTCAACCTTCTATAATTTTTATAG
ATGAAGTTGATAGCCTTTTGTGTGAAAGAAGAGAAGGGGAGCACGATGCTAGTAGACGCCTAAAAACTGAATTTCTAATAGAATTTGATG
GTGTACAGTCTGCTGGAGATGACAGAGTACTTGTAATGGGTGCAACTAATAGGCCACAAGAGCTTGATGAGGCTGTTCTCAGGCGTTTCA
TCAAACGGGTATATGTGTCTTTACCAAATGAGGAGACAAGACTACTTTTGCTTAAAAATCTGTTATGTAAACAAGGAAGTCCATTGACCC
AAAAAGAACTAGCACAACTTGCTAGAATGACTGATGGATACTCAGGAAGTGACCTAACAGCTTTGGCAAAAGATGCAGCACTGGGTCCTA
TCCGAGAACTAAAACCAGAACAGGTGAAGAATATGTCTGCCAGTGAGATGAGAAATATTCGATTATCTGACTTCACTGAATCCTTGAAAA
AAATAAAACGCAGCGTCAGCCCTCAAACTTTAGAAGCGTACATACGTTGGAACAAGGACTTTGGAGATACCACTGTTTAAGGAAATACCT
TTGTAAACCTGCAGAACATTTTACTTAAAAGAGGAAACACAAGATCTTCAATGAACGTCATCGGCTACAGAAACAGCCTAAGTTTACAGG
ACTTTTTAGAGTCTTACATATTTGTGCACCAAACTTGAAGATGAACCAGAAAACAGACTTAAACAAAATATACAATGCAAATGTAATTTT
TTGTTGTTTAAGGCCTTGCCTTGATGGTCACAGTTATCCCAATGGACACTAAGTTAGAGCACAACAAAACCTGATTCTGGTCTTCTTTAC
CAATATAATCATAATGTAAATAATAATTTGTATATTGTGTTGCAGATGAAAGTATTCCAGGAACAGTGAATGGTAGAAGACACAAGAACA
TTTGTTTGTTTGTCTTCTGATGTTTTTTCTTAAAATAGTAATTTCTCCTACTTTTCTTTTCTACTGTTGTCTTAACTACAGGTGATTGGA
ATGCCAAACACTCTTAAGTTTATTTTCTTTTTTCGTTTTATAAATTCAGTGTGCCAAATGAAACTTTTTTCCTAAGTAACTGTAATAGGA
AAAAGTTTATTTTGAGAGTTTCTTCTTCATAAATCTACAGACATTAAACAATTGTTGTGTTCTTTTTACCTTTTATTTTTCTATTACCTT
GCTACCAAACAGTTTAGATAGCAATATAATAGCAAAAAAGCAAATATGGTAAAATAGAGAAGGTTTGAAGGTTTGAGTTACTCTGTCATA
TAACATGTAGATCAGTCTTCATGTGACCTGCAGTATTTTTTTTTCTAATGTATTTGTCAGAAATCTGTTGTAGACTGTTAACTTCTTCCT
GATGGAATTTATTTTCTGCAAGAATTATTCTGATATTTAAGAGAGCCAATTTTAACTGCTGTGAAAATGTTTCCAGTGCAAGAGAAGGGA
AATACTAGGAACTAAGACATTTCTAATTTATTGCTTATTACTTTCTTAATTTTACAGGATAATTATAAGCAAGTGGAACTACCATCTTTT
ATTCTTAATAATTATTAATCCCTTCAATGAAACTTTAAAAAAACTGAATTTTTATACATGGCATACATTTTTCTAGTTCCTTCTGCTTGC
TTTATTAACTCAAAAGTTCTAGTTCTAGTCTGTTGATCTGCCTTTTGTTCTCCCAAAATGTACAGTAATTCCATTTGTTTGTATAAATAT
GCCTGGATTTTCATTATAAAAATGTCATTGTAGGGAGTAGAGACTCATATCATGGCCTTTTAAATATTGTAATAAAGGCAAATAGATATT
TGCCCTTAGTTTACTGGTTAAAAGTTTGTTTACAGAACTTTTCTCTGGTGCTTAAATGATGCTATGTAAAATGTCATGAGTGGAAAGAAT
ATTTGTAGTAGTAACAAGAATTTTTCATTTAGGAAAGATTTCTTAGGTTTTGAAAGAATACATTAAAATAAAAAACTTGCCCCTACTAGG
TAAGAACTTTATAATGAAGACATACATTCTTCTTAATTTTACTCTTGCTCTTGTTAAAGATTTGTTTGAATATAGAAGATGCATGATTTC
TGGGTTTTTTTTTTTTTTTGAGACAGAGTTTCGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCAATCTCGACTCACCACAACCTCCGC
CTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGCCACTACCCCAGCTAATTTTGTATTTTT
AGTAGAGATGGGGTTTCTCCATGTTGGTCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTGG
GATTACAGGCATAAGCCACTGCGCCCAGCCAGAAGATGCATGATTTCTTAGGATCATATGCTGTTTGTAGCCATAAGGTAAATCATGTCT
CTTCCAATCATGACTTTGGAACTCCCTGAATAATAAAAATGAGAGTTGAGATAAATAGGGGAAAAAAAATTTTTTTCAAGCCAGAGCTAT
GCATATGTTAGGTGATGGGTAGTATCCCTTTAAGGTCTCAAACATTACAACATCAATTATGAAATACTGATAACGAAAGGTAGTAATGAA
ATATATATGATGAAAAGAATTGAGAAGTTCTAAATTAAGACATTTCAGTTAAGCTCATAAAATTTCATTGTTTTCATTTAAAAGATTAAC
GTTATTGATACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGGTTCCAGCTCAACTTTTAATATATTGTCTCCTTTAAAACTATC
ATGGTTATAATTCTATTGGGAAAGACTTTTAGATAACAAAGATTTCAAATGTTAAAAGAGATAAAAGTCAGGTTAATACTATCTTAAACA
CTGAGTCAGAAAATCATTACTGTATAGAAGTTGCTTTCCTGATCAAGTCTGAACTTCAGCTAGTGCTAGAGAACTATTTTCTATGACTTA
ACTCTAACCAAGTTTTATTTTAAGCTGTTTCTTTGATAGAAGGGCCATGAAAATAGAGTAATGATATAGTAGGAGATAAGGGATTGGTTT
GGTCTTTTTCAATAAAGATAGAAGTTGCTGAAGTTTTCTGAATTAATAATGACTTAGATTGTGACCTTTTAGATTCGGTGTTGAGCTCTG
TGTTGTATTACTTCCTAAAAGATAATGCTTAAACATTAAGCATTAGTGTGCTCTTCATGTTAATATGGCAGAGTTTTGTAAACTAAATTA
AAACTTACTGATATATTGGACTTTGAGCCAAGGGAAAGAATGAGTACTATCTTTCCAGATATCTTAAGGGTAAAAGCTTATTCTAAGACA
GTCTGTCCATTGAGAATATTAGATTTCTGACTTGCAAATATGTTTGTACTCCAGAAGAATTAGAGGAAAAGCAGATACTAGAATTCTAAT
TTAATTACATATACAGCCGTCTTTGTTTATAGTGTAGAATTCTTTATATTTTGTACAAAAACTAATTCTTTTGGTAAAATGAACCATTTA
CAGTTCGGTTTTGGACTCTGAGTCAAAGGATTTTCCTTTAAATGCTTGTCTCAATTTTAGTCTGGTCTTTTGTACTTTTCTTCAGAAGAA

>9768_9768_1_BIRC6-SPAST_BIRC6_chr2_32631625_ENST00000421745_SPAST_chr2_32339707_ENST00000315285_length(amino acids)=881AA_BP=492
MVTGGGAAPPGTVTEPLPSVIVLSAGRKMAAAAAAASGPGCSSAAGAGAAGVSEWLVLRDGCMHCDADGLHSLSYHPALNAILAVTSRGT
IKVIDGTSGATLQASALSAKPGGQVKCQYISAVDKVIFVDDYAVGCRKDLNGILLLDTALQTPVSKQDDVVQLELPVTEAQQLLSACLEK
VDISSTEGYDLFITQLKDGLKNTSHETAANHKVAKWATVTFHLPHHVLKSIASAIVNELKKINQNVAALPVASSVMDRLSYLLPSARPEL
GVGPGRSVDRSLMYSEANRRETFTSWPHVGYRWAQPDPMAQAGFYHQPASSGDDRAMCFTCSVCLVCWEPTDEPWSEHERHSPNCPFVKG
EHTQNVPLSVTLATSPAQFPCTDGTDRISCFGSGSCPHFLAAATKRGKICIWDVSKLMKVHLKFEINAYDPAIVQQLILSGDPSSGVDSR
RPTLAWLEDSSSCSDIPKLEGDSDDLLEDSDSEEHSRSDSVTESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMV
SGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRP
ELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEH
DASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYS

--------------------------------------------------------------
>9768_9768_2_BIRC6-SPAST_BIRC6_chr2_32631625_ENST00000421745_SPAST_chr2_32339707_ENST00000345662_length(transcript)=5920nt_BP=1611nt
TCCCTGCTTCTCCCCCTCTCCCGTCAGCCTCCCTCCGAGTTTGGCCCCTCCGGCCGGGCGATCGACGTTCCGCGTGCGTGCGGGCGCCTG
ACTTCACTTCCGGCTAACGCGCTCGGCTTGCCCCCTGGCCCCGGATGGTGACTGGTGGTGGTGCTGCACCTCCCGGGACTGTCACTGAGC
CGCTTCCCAGTGTGATTGTGCTGAGCGCAGGCCGGAAGATGGCGGCTGCGGCTGCGGCGGCCTCGGGCCCCGGCTGCTCCTCGGCGGCGG
GGGCGGGGGCGGCCGGGGTCTCAGAGTGGCTGGTGCTGCGGGACGGCTGCATGCACTGCGACGCCGACGGGCTGCACAGCCTGTCCTACC
ACCCTGCGCTCAACGCCATCCTGGCCGTCACTAGCCGCGGGACCATCAAAGTCATCGACGGCACCTCGGGGGCCACACTGCAGGCCTCCG
CGCTCAGTGCTAAACCAGGTGGACAGGTGAAATGTCAGTATATCTCTGCTGTGGATAAAGTTATATTTGTGGATGATTATGCAGTAGGGT
GTAGGAAGGACCTTAATGGAATCTTGTTGTTAGACACTGCTCTGCAAACTCCAGTTTCAAAGCAGGATGATGTGGTTCAGCTTGAATTAC
CCGTTACAGAGGCACAGCAGCTCTTATCAGCATGTTTAGAAAAGGTAGATATTTCTAGTACAGAGGGTTATGATTTGTTCATCACACAGC
TCAAAGATGGTTTAAAAAATACATCTCATGAGACTGCAGCAAACCACAAAGTTGCTAAGTGGGCCACAGTTACATTTCATCTTCCTCATC
ATGTGTTGAAGTCCATTGCCAGTGCCATTGTAAATGAACTCAAGAAAATAAATCAAAATGTTGCTGCCTTACCTGTGGCGTCCTCAGTGA
TGGACAGATTGTCTTACCTCTTACCTAGTGCACGTCCAGAACTCGGAGTGGGGCCAGGCCGTTCTGTAGACAGATCACTGATGTATAGTG
AAGCTAACAGACGGGAGACATTTACCTCATGGCCTCATGTAGGCTATAGGTGGGCACAACCAGATCCCATGGCTCAAGCTGGATTTTATC
ATCAGCCTGCCTCATCTGGAGATGATAGAGCCATGTGTTTTACTTGTAGTGTATGCCTCGTTTGTTGGGAACCTACTGATGAACCTTGGT
CTGAACACGAAAGACATTCCCCAAACTGCCCATTTGTGAAAGGTGAGCACACACAGAATGTGCCATTGTCAGTCACTCTTGCAACAAGTC
CTGCACAGTTTCCTTGTACGGATGGAACTGACAGAATATCTTGCTTTGGGTCGGGGAGCTGCCCTCATTTTCTAGCTGCTGCAACTAAAC
GAGGAAAGATCTGCATATGGGATGTTTCCAAACTTATGAAGGTGCACTTAAAGTTTGAAATTAATGCCTATGATCCAGCAATTGTACAAC
AGCTTATTCTATCAGGAGACCCAAGCTCAGGAGTTGATTCAAGGAGACCAACTTTGGCGTGGCTGGAGGACTCCTCTAGTTGCTCAGATA
TACCAAAATTGGAAGGAGATAGTGATGATTTACTGGAGGATTCAGACAGTGAAGAGCATTCCAGATCAGATTCTGTGACAGAAAGTGGAG
CTGTTCCAAAAAGAAAAGACCCCTTAACACACACTAGTAATTCACTGCCTCGTTCAAAAACAGTTATGAAAACTGGATCTGCAGGCCTTT
CAGGCCACCATAGAGCACCTAGTTACAGTGGTTTATCCATGGTTTCTGGAGTGAAACAGGGATCTGGTCCTGCTCCTACCACTCATAAGG
GTACTCCGAAAACAAATAGGACAAATAAACCTTCTACCCCTACAACTGCTACTCGTAAGAAAAAAGACTTGAAGAATTTTAGGAATGTGG
ACAGCAACCTTGCTAACCTTATAATGAATGAAATTGTGGACAATGGAACAGCTGTTAAATTTGATGATATAGCTGGTCAAGACTTGGCAA
AACAAGCATTGCAAGAAATTGTTATTCTTCCTTCTCTGAGGCCTGAGTTGTTCACAGGGCTTAGAGCTCCTGCCAGAGGGCTGTTACTCT
TTGGTCCACCTGGGAATGGGAAGACAATGCTGGCTAAAGCAGTAGCTGCAGAATCGAATGCAACCTTCTTTAATATAAGTGCTGCAAGTT
TAACTTCAAAATACGTGGGAGAAGGAGAGAAATTGGTGAGGGCTCTTTTTGCTGTGGCTCGAGAACTTCAACCTTCTATAATTTTTATAG
ATGAAGTTGATAGCCTTTTGTGTGAAAGAAGAGAAGGGGAGCACGATGCTAGTAGACGCCTAAAAACTGAATTTCTAATAGAATTTGATG
GTGTACAGTCTGCTGGAGATGACAGAGTACTTGTAATGGGTGCAACTAATAGGCCACAAGAGCTTGATGAGGCTGTTCTCAGGCGTTTCA
TCAAACGGGTATATGTGTCTTTACCAAATGAGGAGACAAGACTACTTTTGCTTAAAAATCTGTTATGTAAACAAGGAAGTCCATTGACCC
AAAAAGAACTAGCACAACTTGCTAGAATGACTGATGGATACTCAGGAAGTGACCTAACAGCTTTGGCAAAAGATGCAGCACTGGGTCCTA
TCCGAGAACTAAAACCAGAACAGGTGAAGAATATGTCTGCCAGTGAGATGAGAAATATTCGATTATCTGACTTCACTGAATCCTTGAAAA
AAATAAAACGCAGCGTCAGCCCTCAAACTTTAGAAGCGTACATACGTTGGAACAAGGACTTTGGAGATACCACTGTTTAAGGAAATACCT
TTGTAAACCTGCAGAACATTTTACTTAAAAGAGGAAACACAAGATCTTCAATGAACGTCATCGGCTACAGAAACAGCCTAAGTTTACAGG
ACTTTTTAGAGTCTTACATATTTGTGCACCAAACTTGAAGATGAACCAGAAAACAGACTTAAACAAAATATACAATGCAAATGTAATTTT
TTGTTGTTTAAGGCCTTGCCTTGATGGTCACAGTTATCCCAATGGACACTAAGTTAGAGCACAACAAAACCTGATTCTGGTCTTCTTTAC
CAATATAATCATAATGTAAATAATAATTTGTATATTGTGTTGCAGATGAAAGTATTCCAGGAACAGTGAATGGTAGAAGACACAAGAACA
TTTGTTTGTTTGTCTTCTGATGTTTTTTCTTAAAATAGTAATTTCTCCTACTTTTCTTTTCTACTGTTGTCTTAACTACAGGTGATTGGA
ATGCCAAACACTCTTAAGTTTATTTTCTTTTTTCGTTTTATAAATTCAGTGTGCCAAATGAAACTTTTTTCCTAAGTAACTGTAATAGGA
AAAAGTTTATTTTGAGAGTTTCTTCTTCATAAATCTACAGACATTAAACAATTGTTGTGTTCTTTTTACCTTTTATTTTTCTATTACCTT
GCTACCAAACAGTTTAGATAGCAATATAATAGCAAAAAAGCAAATATGGTAAAATAGAGAAGGTTTGAAGGTTTGAGTTACTCTGTCATA
TAACATGTAGATCAGTCTTCATGTGACCTGCAGTATTTTTTTTTCTAATGTATTTGTCAGAAATCTGTTGTAGACTGTTAACTTCTTCCT
GATGGAATTTATTTTCTGCAAGAATTATTCTGATATTTAAGAGAGCCAATTTTAACTGCTGTGAAAATGTTTCCAGTGCAAGAGAAGGGA
AATACTAGGAACTAAGACATTTCTAATTTATTGCTTATTACTTTCTTAATTTTACAGGATAATTATAAGCAAGTGGAACTACCATCTTTT
ATTCTTAATAATTATTAATCCCTTCAATGAAACTTTAAAAAAACTGAATTTTTATACATGGCATACATTTTTCTAGTTCCTTCTGCTTGC
TTTATTAACTCAAAAGTTCTAGTTCTAGTCTGTTGATCTGCCTTTTGTTCTCCCAAAATGTACAGTAATTCCATTTGTTTGTATAAATAT
GCCTGGATTTTCATTATAAAAATGTCATTGTAGGGAGTAGAGACTCATATCATGGCCTTTTAAATATTGTAATAAAGGCAAATAGATATT
TGCCCTTAGTTTACTGGTTAAAAGTTTGTTTACAGAACTTTTCTCTGGTGCTTAAATGATGCTATGTAAAATGTCATGAGTGGAAAGAAT
ATTTGTAGTAGTAACAAGAATTTTTCATTTAGGAAAGATTTCTTAGGTTTTGAAAGAATACATTAAAATAAAAAACTTGCCCCTACTAGG
TAAGAACTTTATAATGAAGACATACATTCTTCTTAATTTTACTCTTGCTCTTGTTAAAGATTTGTTTGAATATAGAAGATGCATGATTTC
TGGGTTTTTTTTTTTTTTTGAGACAGAGTTTCGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCAATCTCGACTCACCACAACCTCCGC
CTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGCCACTACCCCAGCTAATTTTGTATTTTT
AGTAGAGATGGGGTTTCTCCATGTTGGTCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTGG
GATTACAGGCATAAGCCACTGCGCCCAGCCAGAAGATGCATGATTTCTTAGGATCATATGCTGTTTGTAGCCATAAGGTAAATCATGTCT
CTTCCAATCATGACTTTGGAACTCCCTGAATAATAAAAATGAGAGTTGAGATAAATAGGGGAAAAAAAATTTTTTTCAAGCCAGAGCTAT
GCATATGTTAGGTGATGGGTAGTATCCCTTTAAGGTCTCAAACATTACAACATCAATTATGAAATACTGATAACGAAAGGTAGTAATGAA
ATATATATGATGAAAAGAATTGAGAAGTTCTAAATTAAGACATTTCAGTTAAGCTCATAAAATTTCATTGTTTTCATTTAAAAGATTAAC
GTTATTGATACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGGTTCCAGCTCAACTTTTAATATATTGTCTCCTTTAAAACTATC
ATGGTTATAATTCTATTGGGAAAGACTTTTAGATAACAAAGATTTCAAATGTTAAAAGAGATAAAAGTCAGGTTAATACTATCTTAAACA
CTGAGTCAGAAAATCATTACTGTATAGAAGTTGCTTTCCTGATCAAGTCTGAACTTCAGCTAGTGCTAGAGAACTATTTTCTATGACTTA
ACTCTAACCAAGTTTTATTTTAAGCTGTTTCTTTGATAGAAGGGCCATGAAAATAGAGTAATGATATAGTAGGAGATAAGGGATTGGTTT
GGTCTTTTTCAATAAAGATAGAAGTTGCTGAAGTTTTCTGAATTAATAATGACTTAGATTGTGACCTTTTAGATTCGGTGTTGAGCTCTG
TGTTGTATTACTTCCTAAAAGATAATGCTTAAACATTAAGCATTAGTGTGCTCTTCATGTTAATATGGCAGAGTTTTGTAAACTAAATTA
AAACTTACTGATATATTGGACTTTGAGCCAAGGGAAAGAATGAGTACTATCTTTCCAGATATCTTAAGGGTAAAAGCTTATTCTAAGACA
GTCTGTCCATTGAGAATATTAGATTTCTGACTTGCAAATATGTTTGTACTCCAGAAGAATTAGAGGAAAAGCAGATACTAGAATTCTAAT
TTAATTACATATACAGCCGTCTTTGTTTATAGTGTAGAATTCTTTATATTTTGTACAAAAACTAATTCTTTTGGTAAAATGAACCATTTA
CAGTTCGGTTTTGGACTCTGAGTCAAAGGATTTTCCTTTAAATGCTTGTCTCAATTTTAGTCTGGTCTTTTGTACTTTTCTTCAGAAGAA

>9768_9768_2_BIRC6-SPAST_BIRC6_chr2_32631625_ENST00000421745_SPAST_chr2_32339707_ENST00000345662_length(amino acids)=881AA_BP=492
MVTGGGAAPPGTVTEPLPSVIVLSAGRKMAAAAAAASGPGCSSAAGAGAAGVSEWLVLRDGCMHCDADGLHSLSYHPALNAILAVTSRGT
IKVIDGTSGATLQASALSAKPGGQVKCQYISAVDKVIFVDDYAVGCRKDLNGILLLDTALQTPVSKQDDVVQLELPVTEAQQLLSACLEK
VDISSTEGYDLFITQLKDGLKNTSHETAANHKVAKWATVTFHLPHHVLKSIASAIVNELKKINQNVAALPVASSVMDRLSYLLPSARPEL
GVGPGRSVDRSLMYSEANRRETFTSWPHVGYRWAQPDPMAQAGFYHQPASSGDDRAMCFTCSVCLVCWEPTDEPWSEHERHSPNCPFVKG
EHTQNVPLSVTLATSPAQFPCTDGTDRISCFGSGSCPHFLAAATKRGKICIWDVSKLMKVHLKFEINAYDPAIVQQLILSGDPSSGVDSR
RPTLAWLEDSSSCSDIPKLEGDSDDLLEDSDSEEHSRSDSVTESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMV
SGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRP
ELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEH
DASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYS

--------------------------------------------------------------
>9768_9768_3_BIRC6-SPAST_BIRC6_chr2_32774548_ENST00000421745_SPAST_chr2_32372287_ENST00000315285_length(transcript)=16583nt_BP=13278nt
TCCCTGCTTCTCCCCCTCTCCCGTCAGCCTCCCTCCGAGTTTGGCCCCTCCGGCCGGGCGATCGACGTTCCGCGTGCGTGCGGGCGCCTG
ACTTCACTTCCGGCTAACGCGCTCGGCTTGCCCCCTGGCCCCGGATGGTGACTGGTGGTGGTGCTGCACCTCCCGGGACTGTCACTGAGC
CGCTTCCCAGTGTGATTGTGCTGAGCGCAGGCCGGAAGATGGCGGCTGCGGCTGCGGCGGCCTCGGGCCCCGGCTGCTCCTCGGCGGCGG
GGGCGGGGGCGGCCGGGGTCTCAGAGTGGCTGGTGCTGCGGGACGGCTGCATGCACTGCGACGCCGACGGGCTGCACAGCCTGTCCTACC
ACCCTGCGCTCAACGCCATCCTGGCCGTCACTAGCCGCGGGACCATCAAAGTCATCGACGGCACCTCGGGGGCCACACTGCAGGCCTCCG
CGCTCAGTGCTAAACCAGGTGGACAGGTGAAATGTCAGTATATCTCTGCTGTGGATAAAGTTATATTTGTGGATGATTATGCAGTAGGGT
GTAGGAAGGACCTTAATGGAATCTTGTTGTTAGACACTGCTCTGCAAACTCCAGTTTCAAAGCAGGATGATGTGGTTCAGCTTGAATTAC
CCGTTACAGAGGCACAGCAGCTCTTATCAGCATGTTTAGAAAAGGTAGATATTTCTAGTACAGAGGGTTATGATTTGTTCATCACACAGC
TCAAAGATGGTTTAAAAAATACATCTCATGAGACTGCAGCAAACCACAAAGTTGCTAAGTGGGCCACAGTTACATTTCATCTTCCTCATC
ATGTGTTGAAGTCCATTGCCAGTGCCATTGTAAATGAACTCAAGAAAATAAATCAAAATGTTGCTGCCTTACCTGTGGCGTCCTCAGTGA
TGGACAGATTGTCTTACCTCTTACCTAGTGCACGTCCAGAACTCGGAGTGGGGCCAGGCCGTTCTGTAGACAGATCACTGATGTATAGTG
AAGCTAACAGACGGGAGACATTTACCTCATGGCCTCATGTAGGCTATAGGTGGGCACAACCAGATCCCATGGCTCAAGCTGGATTTTATC
ATCAGCCTGCCTCATCTGGAGATGATAGAGCCATGTGTTTTACTTGTAGTGTATGCCTCGTTTGTTGGGAACCTACTGATGAACCTTGGT
CTGAACACGAAAGACATTCCCCAAACTGCCCATTTGTGAAAGGTGAGCACACACAGAATGTGCCATTGTCAGTCACTCTTGCAACAAGTC
CTGCACAGTTTCCTTGTACGGATGGAACTGACAGAATATCTTGCTTTGGGTCGGGGAGCTGCCCTCATTTTCTAGCTGCTGCAACTAAAC
GAGGAAAGATCTGCATATGGGATGTTTCCAAACTTATGAAGGTGCACTTAAAGTTTGAAATTAATGCCTATGATCCAGCAATTGTACAAC
AGCTTATTCTATCAGGAGACCCAAGCTCAGGAGTTGATTCAAGGAGACCAACTTTGGCGTGGCTGGAGGACTCCTCTAGTTGCTCAGATA
TACCAAAATTGGAAGGAGATAGTGATGATTTACTGGAGGATTCAGACAGTGAAGAGCATTCCAGATCAGATTCTGTGACAGGGCATACAT
CACAGAAGGAAGCCATGGAAGTAAGCCTTGATATAACAGCACTCAGCATTCTCCAACAGCCAGAAAAACTTCAGTGGGAGATTGTTGCAA
ATGTGCTTGAAGATACTGTTAAGGATCTTGAAGAACTTGGGGCAAATCCTTGTTTAACAAACTCTAAGAGTGAAAAGACAAAGGAAAAGC
ACCAGGAGCAACACAACATTCCTTTTCCATGTTTATTAGCTGGAGGTTTATTAACATATAAATCTCCTGCTACCTCACCCATTAGTAGTA
ATTCTCACAGGTCACTGGATGGTTTAAGCAGAACTCAGGGTGAAAGTATATCAGAACAAGGGTCAACTGACAATGAATCCTGCACTAATT
CAGAACTAAATTCTCCTCTGGTAAGGAGGACTTTACCGGTTTTGCTTCTTTATAGCATCAAGGAATCTGATGAGAAAGCAGGAAAGATCT
TTTCACAGATGAACAATATTATGAGTAAAAGTTTGCATGATGATGGTTTTACTGTTCCACAGATTATTGAAATGGAGCTGGATAGTCAGG
AGCAGTTGTTATTGCAGGATCCTCCTGTGACTTACATTCAGCAATTTGCAGATGCAGCAGCCAACCTTACCTCTCCGGATTCTGAGAAGT
GGAACTCTGTGTTTCCCAAGCCTGGGACTTTGGTTCAGTGCTTGAGGCTGCCAAAGTTTGCAGAGGAGGAGAATCTTTGTATAGACTCAA
TAACTCCTTGTGCTGACGGAATTCATTTGTTGGTAGGACTGCGGACATGCCCTGTTGAATCCTTGAGTGCAATAAATCAAGTAGAGGCCT
TGAATAATTTAAATAAATTAAACTCTGCACTATGTAATAGACGGAAAGGTGAGCTGGAATCAAATCTTGCTGTAGTGAATGGTGCAAATA
TTAGTGTAATCCAACATGAATCACCAGCAGATGTACAGACTCCTTTAATAATTCAGCCTGAGCAGAGGAATGTTAGTGGTGGATATTTAG
TGCTTTATAAAATGAATTATGCCACTCGGATAGTGACTTTAGAAGAGGAGCCAATAAAAATACAACATATCAAAGATCCCCAGGACACAA
TTACCTCGCTCATTTTGCTTCCACCCGATATATTGGATAATCGAGAGGATGACTGTGAGGAACCTATTGAGGACATGCAGTTAACCTCAA
AGAATGGTTTTGAGAGAGAAAAAACGTCTGACATTTCTACTCTTGGACACCTGGTAATAACCACTCAGGGAGGATATGTAAAAATACTAG
ATCTTTCAAACTTTGAAATTTTGGCCAAAGTGGAGCCTCCCAAAAAGGAGGGCACTGAGGAACAGGACACATTTGTTTCTGTGATTTACT
GTTCTGGCACAGACAGGCTGTGTGCATGCACCAAAGGTGGTGAGCTTCATTTTCTCCAAATTGGAGGAACCTGTGATGATATTGATGAAG
CTGATATACTAGTGGATGGATCTCTTTCTAAAGGAATAGAACCATCTTCAGAAGGTTCCAAACCTTTATCAAATCCTTCAAGTCCTGGCA
TTTCAGGAGTTGATTTATTGGTGGACCAGCCATTCACCCTTGAAATCTTGACATCCCTAGTGGAGCTAACCCGCTTTGAGACTTTGACTC
CAAGGTTTTCAGCGACTGTTCCTCCATGCTGGGTAGAAGTTCAACAAGAACAGCAGCAAAGGAGGCATCCTCAACATTTGCATCAGCAAC
ACCATGGTGATGCTGCTCAGCATACTCGAACTTGGAAACTACAGACCGACAGCAACAGCTGGGATGAACATGTATTTGAATTAGTACTAC
CTAAAGCTTGTATGGTTGGACATGTGGACTTCAAATTCGTTTTGAACTCAAACATCACCAATATTCCACAGATACAAGTGACACTGCTGA
AAAATAAAGCTCCAGGATTAGGGAAAGTCAATGCTCTTAACATTGAAGTGGAACAAAATGGGAAACCGTCCCTGGTTGATTTGAATGAAG
AAATGCAGCACATGGATGTAGAGGAATCACAGTGTCTTAGATTATGTCCATTTTTGGAGGATCATAAAGAAGACATTCTATGTGGGCCAG
TATGGCTTGCTAGTGGCCTTGATCTATCAGGGCATGCTGGAATGTTGACGTTAACAAGCCCCAAACTTGTTAAAGGTATGGCAGGAGGAA
AATATCGTTCGTTTTTAATCCATGTCAAGGCAGTGAATGAAAGAGGAACAGAAGAGATTTGTAATGGTGGTATGCGTCCTGTAGTAAGGC
TTCCATCCCTAAAACACCAGAGTAACAAGGGTTATTCACTTGCTTCACTTTTGGCTAAAGTTGCAGCAGGCAAGGAAAAATCATCTAATG
TTAAGAATGAAAATACAAGTGGCACCCGTAAATCTGAAAACCTCCGGGGCTGTGATTTACTTCAAGAGGTCTCAGTCACCATTCGAAGAT
TTAAGAAAACCTCAATTTCTAAGGAAAGAGTGCAACGATGTGCCATGTTACAGTTTTCAGAATTTCATGAGAAGCTTGTTAATACTCTTT
GCAGAAAAACAGATGATGGCCAGATCACAGAACATGCCCAGAGCCTTGTGTTGGATACTCTCTGTTGGTTAGCTGGAGTTCATTCAAATG
GACCCGGAAGCTCAAAGGAAGGAAATGAGAACCTACTTTCAAAAACACGAAAATTTCTGTCAGACATCGTACGTGTTTGCTTCTTTGAGG
CAGGACGAAGTATAGCCCATAAGTGTGCCCGATTTCTAGCCTTGTGCATTAGTAATGGCAAATGTGACCCATGTCAACCAGCATTTGGAC
CTGTTCTGTTGAAGGCTTTACTTGATAATATGTCATTTTTACCTGCAGCAACAACTGGTGGTTCTGTCTATTGGTATTTTGTCTTACTGA
ATTATGTGAAAGATGAAGATCTGGCTGGATGCAGTACAGCTTGTGCATCTTTGCTTACTGCAGTGTCCAGACAGTTACAGGACAGGCTAA
CACCAATGGAGGCTTTACTTCAGACAAGATATGGATTATATAGCTCACCATTTGATCCAGTCCTCTTTGATTTGGAGATGAGTGGCTCTT
CTTGTAAAAATGTTTATAACAGCAGCATTGGTGTCCAGTCAGATGAAATTGATTTATCAGATGTCCTTTCAGGAAATGGAAAGGTCAGTA
GTTGCACAGCTGCTGAGGGTAGTTTCACATCTCTCACTGGACTTTTGGAAGTTGAACCTCTGCACTTTACTTGTGTGTCAACTAGTGATG
GAACCAGAATAGAAAGGGATGATGCAATGAGTTCCTTCGGGGTTACTCCTGCAGTAGGTGGACTATCATCTGGGACAGTTGGGGAAGCCT
CGACAGCCCTGAGTTCAGCAGCCCAGGTAGCTTTGCAGTCTCTCTCTCATGCAATGGCTTCAGCCGAGCAACAGCTACAGGTGCTGCAAG
AGAAACAGCAGCAGCTTTTGAAGCTTCAGCAACAGAAAGCAAAGCTGGAAGCCAAGTTACATCAGACAACAGCTGCAGCAGCTGCAGCAG
CATCAGCAGTAGGTCCTGTTCACAACTCTGTGCCTTCCAACCCAGTGGCTGCCCCTGGATTCTTCATTCATCCATCTGATGTTATTCCAC
CCACTCCAAAAACAACACCTCTTTTTATGACTCCACCACTCACTCCACCCAATGAAGCAGTTTCCGTTGTGATTAATGCCGAACTTGCAC
AGCTTTTCCCAGGCTCAGTCATTGATCCCCCAGCAGTCAATCTTGCTGCACATAACAAAAATTCCAACAAGTCCAGAATGAATCCACTTG
GTTCTGGTCTAGCCCTTGCAATTTCTCATGCTTCACATTTTCTTCAACCTCCGCCTCACCAGTCCATTATTATAGAGCGAATGCATTCAG
GAGCAAGAAGATTTGTGACCTTGGATTTTGGGAGGCCTATATTGTTGACTGATGTATTGATTCCCACTTGTGGAGACTTGGCCTCTTTGT
CAATTGACATTTGGACATTAGGAGAAGAGGTGGATGGAAGGCGGTTGGTAGTGGCAACTGATATAAGCACTCATTCACTAATTCTTCATG
ACTTAATACCACCTCCCGTGTGCAGATTCATGAAGATCACTGTTATTGGACGTTACGGGAGTACAAATGCCAGAGCCAAAATCCCATTAG
GATTTTACTATGGTCATACCTACATCTTGCCTTGGGAAAGTGAACTGAAGTTAATGCATGATCCTCTAAAGGGAGAGGGAGAATCTGCAA
ACCAGCCAGAAATTGACCAGCATTTAGCAATGATGGTTGCTTTGCAGGAGGATATACAGTGCAGGTATAACTTGGCTTGTCATCGTCTGG
AAACCCTTTTGCAAAGTATTGATCTTCCTCCTCTAAACAGTGCTAACAATGCACAGTACTTTTTACGAAAACCAGATAAGGCAGTTGAGG
AAGACAGTAGGGTTTTTTCTGCTTATCAAGATTGTATTCAGCTACAGCTTCAACTAAATTTGGCTCATAATGCAGTGCAGAGGCTCAAAG
TGGCGCTAGGTGCAAGCCGGAAGATGTTGAGTGAAACATCAAATCCAGAAGATTTAATTCAGACATCTTCCACAGAGCAGTTACGTACTA
TCATCAGATATTTACTGGACACTTTGCTCAGCCTGCTTCATGCTTCTAATGGACACTCTGTTCCTGCAGTTTTGCAGAGCACATTTCATG
CCCAGGCCTGTGAAGAGCTCTTTAAACACTTGTGCATCAGTGGAACCCCAAAGATACGGTTACATACTGGTCTTCTTCTTGTTCAACTGT
GTGGTGGTGAAAGGTGGTGGGGTCAATTTCTTTCTAATGTCCTTCAGGAATTGTACAATTCGGAACAGCTTCTCATCTTTCCACAGGATA
GGGTCTTCATGTTACTTTCCTGCATTGGTCAAAGATCACTTAGTAATAGTGGAGTATTAGAAAGCTTACTTAATCTCTTGGATAATTTAT
TGTCACCTCTTCAGCCACAGTTACCCATGCATAGGAGGACAGAAGGAGTACTAGATATTCCCATGATCAGTTGGGTTGTTATGCTGGTGT
CCAGGTTGCTGGATTATGTGGCAACTGTTGAAGATGAAGCAGCAGCTGCAAAGAAACCTTTGAATGGTAATCAGTGGAGTTTTATTAACA
ATAATCTACACACTCAGAGCTTAAATAGATCTTCTAAAGGCAGCAGTAGCCTTGATAGATTATATTCCAGAAAAATCAGAAAGCAGCTTG
TTCATCATAAACAGCAACTTAACCTACTAAAAGCAAAGCAGAAGGCATTGGTAGAACAGATGGAAAAAGAAAAAATACAAAGTAACAAAG
GATCATCATATAAACTCCTGGTAGAACAAGCAAAACTAAAGCAGGCCACTTCAAAGCACTTTAAGGATTTAATTCGTTTACGTCGGACAG
CAGAATGGTCCCGTTCTAATTTAGACACAGAAGTTACAACAGCAAAAGAAAGTCCTGAGATAGAACCACTTCCATTTACTCTGGCCCATG
AGCGTTGTATCTCAGTAGTCCAGAAACTTGTTCTGTTTCTTCTCTCCATGGACTTTACATGTCATGCAGATCTCTTATTGTTTGTTTGTA
AGGTTCTTGCACGCATTGCAAATGCCACGAGGCCAACTATTCATCTGTGTGAGATTGTGAACGAACCCCAGCTGGAAAGACTGCTGTTAC
TTTTGGTTGGAACTGACTTCAATAGAGGAGATATATCTTGGGGTGGTGCTTGGGCTCAGTATTCCTTAACTTGCATGCTACAAGATATTT
TAGCAGGAGAATTACTGGCTCCAGTAGCCGCAGAAGCCATGGAGGAAGGAACAGTGGGTGATGATGTAGGTGCGACAGCTGGTGACTCTG
ATGACTCCCTTCAACAGTCCTCAGTTCAGTTGCTGGAAACTATAGATGAACCTTTGACACATGACATAACAGGTGCACCTCCTCTGTCCT
CTTTGGAAAAAGATAAAGAAATTGACCTTGAGTTACTTCAGGATCTAATGGAAGTTGACATTGATCCTTTAGATATTGATTTGGAAAAGG
ACCCTCTTGCAGCCAAGGTTTTTAAGCCAATAAGCAGTACATGGTATGATTATTGGGGTGCTGATTATGGGACCTACAATTACAACCCTT
ACATTGGAGGTCTGGGAATTCCTGTAGCAAAGCCACCAGCAAACACGGAGAAGAACGGATCACAGACAGTTAGCGTTTCAGTCTCTCAGG
CCCTGGATGCTCGCCTAGAAGTTGGACTTGAACAGCAAGCAGAACTGATGTTGAAAATGATGTCTACTCTGGAGGCAGATTCCATTTTAC
AGGCATTAACAAATACATCTCCTACATTATCACAGTCTCCCACTGGAACAGATGATTCACTTCTAGGGGGTTTACAAGCAGCAAACCAAA
CCAGCCAGCTTATTATACAGTTATCATCTGTCCCAATGTTAAATGTTTGTTTCAACAAACTTTTTTCCATGCTTCAAGTCCATCATGTTC
AGTTGGAGTCACTTCTCCAATTGTGGCTCACACTGAGCCTGAATTCTAGTTCAACTGGAAACAAAGAAAATGGAGCAGACATATTTTTAT
ATAATGCTAATAGGATACCTGTTATTTCATTAAATCAAGCATCAATAACTAGCTTTCTCACAGTGTTAGCTTGGTATCCCAATACTTTGC
TCCGGACATGGTGCCTTGTGCTTCATAGCCTAACACTCATGACAAACATGCAGCTTAATTCTGGTTCCAGCAGTGCCATTGGAACTCAGG
AGAGTACTGCTCATTTGTTGGTTTCAGATCCAAACCTAATTCATGTATTAGTGAAATTTCTTTCTGGCACCAGTCCACATGGAACAAATC
AACACAGTCCACAGGTTGGTCCTACAGCTACACAAGCTATGCAAGAATTTCTTACTCGATTACAAGTGCATCTTTCTTCAACATGTCCTC
AGATATTCAGTGAATTTTTGCTCAAGCTAATTCATATACTTTCAACTGAAAGGGGTGCTTTCCAGACAGGCCAAGGACCTCTCGATGCCC
AAGTGAAGCTCTTAGAATTCACTCTGGAGCAGAATTTTGAAGTCGTTTCAGTTAGTACTATTTCTGCCGTGATAGAATCGGTTACATTTT
TAGTGCACCACTATATCACTTGCTCAGACAAAGTAATGTCAAGAAGTGGATCAGATAGCTCCGTGGGTGCTCGAGCATGCTTTGGGGGAC
TCTTTGCCAATCTTATTCGTCCGGGTGATGCAAAAGCAGTTTGTGGCGAAATGACAAGAGATCAACTCATGTTTGATTTGTTAAAACTTG
TTAACATTTTAGTGCAGCTGCCTCTTTCAGGCAATAGGGAATACAGTGCAAGAGTGTCTGTGACCACAAATACAACAGATAGTGTTTCAG
ATGAAGAAAAAGTCTCAGGAGGCAAAGATGGCAATGGAAGCAGTACCAGTGTTCAAGGATCGCCTGCATATGTTGCTGACTTAGTCTTAG
CCAACCAACAAATTATGAGCCAGATTTTGTCTGCTCTGGGCCTGTGTAATAGCAGTGCCATGGCAATGATAATTGGAGCAAGTGGATTAC
ATCTCACTAAACATGAAAACTTTCATGGTGGGTTGGATGCCATATCAGTTGGGGATGGATTATTTACCATACTGACAACCCTTAGTAAAA
AAGCTTCTACAGTCCACATGATGCTGCAGCCAATTTTAACATACATGGCCTGTGGATATATGGGCAGACAAGGCTCTCTTGCTACTTGCC
AGTTATCTGAGCCATTATTGTGGTTCATTTTGAGAGTATTGGATACTAGTGATGCCTTGAAAGCATTTCATGATATGGGTGGTGTTCAGC
TCATATGCAATAATATGGTTACTAGTACAAGGGCTATTGTGAACACTGCAAGAAGTATGGTATCAACTATTATGAAATTTCTTGACTCTG
GTCCAAATAAAGCTGTTGACAGCACATTGAAAACAAGAATACTAGCTTCTGAGCCTGACAATGCTGAAGGGATTCATAACTTTGCACCCC
TCGGTACAATCACATCTAGCAGTCCTACTGCCCAACCAGCTGAAGTGCTATTGCAGGCCACACCTCCTCACAGAAGAGCTCGCTCTGCTG
CTTGGTCCTACATCTTTCTTCCAGAGGAGGCTTGGTGTGACCTTACCATTCACCTTCCTGCAGCAGTGCTGCTTAAGGAGATACATATCC
AGCCTCATCTTGCATCTCTTGCAACCTGCCCTTCCTCAGTGTCTGTTGAAGTAAGTGCAGATGGGGTAAATATGCTACCTTTGTCCACTC
CTGTTGTCACAAGTGGCCTCACCTACATAAAAATTCAGCTTGTAAAAGCCGAAGTAGCTTCTGCTGTCTGCCTTAGACTACATCGTCCAC
GGGATGCCAGCACATTAGGCCTTTCACAAATTAAATTATTGGGGCTCACTGCTTTTGGTACCACCTCTTCTGCAACAGTTAATAATCCAT
TCCTTCCATCTGAAGATCAGGTATCCAAAACAAGTATTGGATGGTTACGGTTATTACATCATTGCCTTACTCACATAAGTGATCTAGAAG
GAATGATGGCAAGTGCAGCTGCACCTACTGCTAATCTGCTGCAGACTTGTGCGGCCTTATTGATGTCACCTTACTGTGGAATGCATTCAC
CCAACATCGAGGTTGTGCTTGTAAAGATAGGACTGCAGTCTACTAGAATTGGCCTGAAGCTCATAGACATTCTCCTGAGAAATTGTGCAG
CATCAGGCAGTGATCCTACAGATTTGAATAGTCCTTTACTTTTTGGAAGACTAAATGGACTCTCTTCTGACTCTACGATAGATATTCTTT
ACCAGCTTGGAACAACTCAGGATCCTGGTACAAAAGACAGAATTCAGGCCTTGTTAAAATGGGTTAGTGATTCTGCAAGAGTGGCTGCTA
TGAAGAGAAGTGGCAGGATGAACTACATGTGTCCTAACTCCTCAACAGTAGAGTATGGTCTTCTGATGCCATCTCCTTCTCATTTGCACT
GTGTAGCAGCCATTCTGTGGCATAGTTATGAGCTGCTTGTAGAATATGACTTACCAGCACTCCTGGACCAAGAGCTCTTTGAGTTACTTT
TTAATTGGTCCATGTCTCTTCCCTGCAATATGGTTTTGAAGAAAGCTGTTGACAGTCTACTTTGCTCAATGTGTCACGTACACCCAAACT
ATTTTTCTTTGCTCATGGGCTGGATGGGAATTACCCCTCCTCCAGTGCAATGTCATCATAGACTGTCCATGACAGATGATAGCAAAAAGC
AGGATCTTAGTTCATCTTTAACAGATGACTCTAAAAATGCACAAGCACCTCTCGCATTAACTGAATCACATTTGGCTACCCTTGCTTCCT
CTTCTCAATCTCCTGAAGCTATTAAACAATTACTAGACTCAGGTTTGCCTTCTCTTCTTGTGAGGAGTCTGGCTAGTTTCTGCTTTAGCC
ACATTTCTAGCTCAGAAAGCATTGCCCAGTCAATAGATATTTCCCAGGACAAACTCAGGCGCCATCATGTCCCACAACAATGTAATAAGA
TGCCTATCACAGCCGACCTAGTTGCTCCTATTCTTAGGTTTTTGACAGAAGTTGGCAATAGCCATATTATGAAAGATTGGCTTGGTGGTT
CTGAAGTCAATCCACTATGGACAGCACTTCTGTTTTTATTGTGTCACTCTGGGTCCACTTCTGGAAGCCATAATTTAGGTGCACAACAGA
CCAGTGCAAGATCAGCTTCTCTTTCTTCAGCTGCTACAACAGGACTGACTACTCAACAGCGCACAGCAATTGAGAATGCAACTGTTGCGT
TCTTTCTACAGTGCATTTCATGCCATCCTAATAATCAAAAGCTGATGGCACAGGTTCTTTGTGAACTATTTCAGACATCTCCTCAAAGAG
GGAACCTTCCAACATCTGGGAACATTTCAGGGTTTATACGAAGATTATTTTTACAGTTGATGCTGGAAGATGAGAAAGTGACAATGTTTC
TTCAGTCTCCATGTCCACTGTACAAAGGTAGAATTAATGCTACTAGCCACGTCATCCAGCATCCAATGTATGGAGCAGGCCACAAATTCC
GTACTCTTCATTTGCCAGTCTCAACAACATTATCAGATGTTCTTGACAGAGTGTCAGATACTCCAAGTATCACAGCTAAATTAATTAGTG
AACAAAAAGATGACAAAGAAAAGAAAAACCATGAAGAGAAAGAAAAAGTTAAAGCGGAAAATGGATTTCAAGACAATTACAGTGTTGTTG
TTGCCTCTGGGCTGAAGTCTCAATCTAAACGTGCTGTGTCAGCTACACCACCTCGCCCACCATCCAGGAGGGGGAGGACAATACCTGATA
AAATAGGAAGTACTTCAGGAGCAGAGGCTGCCAACAAAATAATTACTGTCCCAGTGTTTCACCTGTTTCACAAACTCTTGGCAGGCCAGC
CATTGCCAGCTGAAATGACACTTGCCCAGCTTTTAACTCTCCTATATGACCGAAAACTTCCTCAGGGTTACCGCTCAATAGATCTGACTG
TTAAATTGGGATCAAGAGTTATAACAGACCCCAGTCTATCAAAAACAGATTCTTATAAAAGACTACACCCTGAAAAAGATCATGGAGACT
TACTTGCTAGCTGTCCAGAAGATGAGGCTCTCACTCCAGGTGATGAATGCATGGATGGGATACTGGATGAATCTTTGCTTGAAACCTGTC
CAATTCAGTCACCATTACAAGTTTTTGCAGGAATGGGTGGACTGGCTCTTATTGCTGAAAGACTACCCATGCTATATCCAGAAGTAATTC
AACAGGTGAGTGCTCCAGTTGTAACATCTACCACTCAGGAAAAGCCGAAGGATAGCGATCAGTTTGAATGGGTGACCATTGAACAGTCAG
GGGAGTTAGTTTATGAAGCACCAGAAACTGTTGCGGCTGAACCTCCACCTATCAAGTCAGCAGTACAGACCATGTCTCCCATACCTGCCC
ATTCTTTGGCTGCTTTTGGATTATTTCTTCGTCTTCCGGGCTATGCGGAAGTGCTACTGAAAGAGAGAAAACATGCCCAGTGCCTTCTTC
GATTGGTATTGGGAGTGACAGATGATGGAGAAGGAAGTCATATTCTTCAATCTCCATCAGCCAATGTGCTTCCAACCCTTCCTTTCCACG
TCCTTCGTAGCTTGTTTAGCACTACACCTTTGACAACTGATGATGGTGTACTTCTAAGGCGGATGGCATTGGAAATTGGAGCCTTACACC
TCATTCTTGTCTGTCTCTCTGCTTTGAGCCACCATTCCCCACGAGTTCCAAACTCTAGCGTGAATCAAACTGAGCCACAGGTGTCAAGCT
CTCATAACCCTACATCAACAGAAGAACAACAGTTATATTGGGCCAAAGGGACTGGCTTTGGAACAGGCTCTACAGCTTCTGGGTGGGATG
TGGAACAAGCCTTAACTAAGCAAAGGCTGGAAGAGGAACATGTTACCTGCCTTCTGCAGGTTCTTGCCAGTTACATAAATCCCGTCAGTA
GTGCGGTAAATGGAGAAGCTCAGTCATCTCATGAGACTAGAGGGCAGAACAGTAATGCCCTTCCTTCTGTACTTCTCGAGCTTCTCAGTC
AGTCCTGCCTCATCCCAGCCATGTCATCTTATCTACGAAATGATTCAGAACTAAAACCAGAACAGGTGAAGAATATGTCTGCCAGTGAGA
TGAGAAATATTCGATTATCTGACTTCACTGAATCCTTGAAAAAAATAAAACGCAGCGTCAGCCCTCAAACTTTAGAAGCGTACATACGTT
GGAACAAGGACTTTGGAGATACCACTGTTTAAGGAAATACCTTTGTAAACCTGCAGAACATTTTACTTAAAAGAGGAAACACAAGATCTT
CAATGAACGTCATCGGCTACAGAAACAGCCTAAGTTTACAGGACTTTTTAGAGTCTTACATATTTGTGCACCAAACTTGAAGATGAACCA
GAAAACAGACTTAAACAAAATATACAATGCAAATGTAATTTTTTGTTGTTTAAGGCCTTGCCTTGATGGTCACAGTTATCCCAATGGACA
CTAAGTTAGAGCACAACAAAACCTGATTCTGGTCTTCTTTACCAATATAATCATAATGTAAATAATAATTTGTATATTGTGTTGCAGATG
AAAGTATTCCAGGAACAGTGAATGGTAGAAGACACAAGAACATTTGTTTGTTTGTCTTCTGATGTTTTTTCTTAAAATAGTAATTTCTCC
TACTTTTCTTTTCTACTGTTGTCTTAACTACAGGTGATTGGAATGCCAAACACTCTTAAGTTTATTTTCTTTTTTCGTTTTATAAATTCA
GTGTGCCAAATGAAACTTTTTTCCTAAGTAACTGTAATAGGAAAAAGTTTATTTTGAGAGTTTCTTCTTCATAAATCTACAGACATTAAA
CAATTGTTGTGTTCTTTTTACCTTTTATTTTTCTATTACCTTGCTACCAAACAGTTTAGATAGCAATATAATAGCAAAAAAGCAAATATG
GTAAAATAGAGAAGGTTTGAAGGTTTGAGTTACTCTGTCATATAACATGTAGATCAGTCTTCATGTGACCTGCAGTATTTTTTTTTCTAA
TGTATTTGTCAGAAATCTGTTGTAGACTGTTAACTTCTTCCTGATGGAATTTATTTTCTGCAAGAATTATTCTGATATTTAAGAGAGCCA
ATTTTAACTGCTGTGAAAATGTTTCCAGTGCAAGAGAAGGGAAATACTAGGAACTAAGACATTTCTAATTTATTGCTTATTACTTTCTTA
ATTTTACAGGATAATTATAAGCAAGTGGAACTACCATCTTTTATTCTTAATAATTATTAATCCCTTCAATGAAACTTTAAAAAAACTGAA
TTTTTATACATGGCATACATTTTTCTAGTTCCTTCTGCTTGCTTTATTAACTCAAAAGTTCTAGTTCTAGTCTGTTGATCTGCCTTTTGT
TCTCCCAAAATGTACAGTAATTCCATTTGTTTGTATAAATATGCCTGGATTTTCATTATAAAAATGTCATTGTAGGGAGTAGAGACTCAT
ATCATGGCCTTTTAAATATTGTAATAAAGGCAAATAGATATTTGCCCTTAGTTTACTGGTTAAAAGTTTGTTTACAGAACTTTTCTCTGG
TGCTTAAATGATGCTATGTAAAATGTCATGAGTGGAAAGAATATTTGTAGTAGTAACAAGAATTTTTCATTTAGGAAAGATTTCTTAGGT
TTTGAAAGAATACATTAAAATAAAAAACTTGCCCCTACTAGGTAAGAACTTTATAATGAAGACATACATTCTTCTTAATTTTACTCTTGC
TCTTGTTAAAGATTTGTTTGAATATAGAAGATGCATGATTTCTGGGTTTTTTTTTTTTTTTGAGACAGAGTTTCGCTCTTGTTGCCCAGG
CTGGAGTGCAATGGCGCAATCTCGACTCACCACAACCTCCGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGG
ATTACAGGCATGCGCCACTACCCCAGCTAATTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTGGTCAGGCTGGTCTTGAACTCCT
GACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATAAGCCACTGCGCCCAGCCAGAAGATGCATGATTTCT
TAGGATCATATGCTGTTTGTAGCCATAAGGTAAATCATGTCTCTTCCAATCATGACTTTGGAACTCCCTGAATAATAAAAATGAGAGTTG
AGATAAATAGGGGAAAAAAAATTTTTTTCAAGCCAGAGCTATGCATATGTTAGGTGATGGGTAGTATCCCTTTAAGGTCTCAAACATTAC
AACATCAATTATGAAATACTGATAACGAAAGGTAGTAATGAAATATATATGATGAAAAGAATTGAGAAGTTCTAAATTAAGACATTTCAG
TTAAGCTCATAAAATTTCATTGTTTTCATTTAAAAGATTAACGTTATTGATACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGG
TTCCAGCTCAACTTTTAATATATTGTCTCCTTTAAAACTATCATGGTTATAATTCTATTGGGAAAGACTTTTAGATAACAAAGATTTCAA
ATGTTAAAAGAGATAAAAGTCAGGTTAATACTATCTTAAACACTGAGTCAGAAAATCATTACTGTATAGAAGTTGCTTTCCTGATCAAGT
CTGAACTTCAGCTAGTGCTAGAGAACTATTTTCTATGACTTAACTCTAACCAAGTTTTATTTTAAGCTGTTTCTTTGATAGAAGGGCCAT
GAAAATAGAGTAATGATATAGTAGGAGATAAGGGATTGGTTTGGTCTTTTTCAATAAAGATAGAAGTTGCTGAAGTTTTCTGAATTAATA
ATGACTTAGATTGTGACCTTTTAGATTCGGTGTTGAGCTCTGTGTTGTATTACTTCCTAAAAGATAATGCTTAAACATTAAGCATTAGTG
TGCTCTTCATGTTAATATGGCAGAGTTTTGTAAACTAAATTAAAACTTACTGATATATTGGACTTTGAGCCAAGGGAAAGAATGAGTACT
ATCTTTCCAGATATCTTAAGGGTAAAAGCTTATTCTAAGACAGTCTGTCCATTGAGAATATTAGATTTCTGACTTGCAAATATGTTTGTA
CTCCAGAAGAATTAGAGGAAAAGCAGATACTAGAATTCTAATTTAATTACATATACAGCCGTCTTTGTTTATAGTGTAGAATTCTTTATA
TTTTGTACAAAAACTAATTCTTTTGGTAAAATGAACCATTTACAGTTCGGTTTTGGACTCTGAGTCAAAGGATTTTCCTTTAAATGCTTG
TCTCAATTTTAGTCTGGTCTTTTGTACTTTTCTTCAGAAGAAATGAATTAAAGGGTACAGTTGCATAAAGTGGGTTTTTATCCTAATGTA

>9768_9768_3_BIRC6-SPAST_BIRC6_chr2_32774548_ENST00000421745_SPAST_chr2_32372287_ENST00000315285_length(amino acids)=4435AA_BP=4381
MVTGGGAAPPGTVTEPLPSVIVLSAGRKMAAAAAAASGPGCSSAAGAGAAGVSEWLVLRDGCMHCDADGLHSLSYHPALNAILAVTSRGT
IKVIDGTSGATLQASALSAKPGGQVKCQYISAVDKVIFVDDYAVGCRKDLNGILLLDTALQTPVSKQDDVVQLELPVTEAQQLLSACLEK
VDISSTEGYDLFITQLKDGLKNTSHETAANHKVAKWATVTFHLPHHVLKSIASAIVNELKKINQNVAALPVASSVMDRLSYLLPSARPEL
GVGPGRSVDRSLMYSEANRRETFTSWPHVGYRWAQPDPMAQAGFYHQPASSGDDRAMCFTCSVCLVCWEPTDEPWSEHERHSPNCPFVKG
EHTQNVPLSVTLATSPAQFPCTDGTDRISCFGSGSCPHFLAAATKRGKICIWDVSKLMKVHLKFEINAYDPAIVQQLILSGDPSSGVDSR
RPTLAWLEDSSSCSDIPKLEGDSDDLLEDSDSEEHSRSDSVTGHTSQKEAMEVSLDITALSILQQPEKLQWEIVANVLEDTVKDLEELGA
NPCLTNSKSEKTKEKHQEQHNIPFPCLLAGGLLTYKSPATSPISSNSHRSLDGLSRTQGESISEQGSTDNESCTNSELNSPLVRRTLPVL
LLYSIKESDEKAGKIFSQMNNIMSKSLHDDGFTVPQIIEMELDSQEQLLLQDPPVTYIQQFADAAANLTSPDSEKWNSVFPKPGTLVQCL
RLPKFAEEENLCIDSITPCADGIHLLVGLRTCPVESLSAINQVEALNNLNKLNSALCNRRKGELESNLAVVNGANISVIQHESPADVQTP
LIIQPEQRNVSGGYLVLYKMNYATRIVTLEEEPIKIQHIKDPQDTITSLILLPPDILDNREDDCEEPIEDMQLTSKNGFEREKTSDISTL
GHLVITTQGGYVKILDLSNFEILAKVEPPKKEGTEEQDTFVSVIYCSGTDRLCACTKGGELHFLQIGGTCDDIDEADILVDGSLSKGIEP
SSEGSKPLSNPSSPGISGVDLLVDQPFTLEILTSLVELTRFETLTPRFSATVPPCWVEVQQEQQQRRHPQHLHQQHHGDAAQHTRTWKLQ
TDSNSWDEHVFELVLPKACMVGHVDFKFVLNSNITNIPQIQVTLLKNKAPGLGKVNALNIEVEQNGKPSLVDLNEEMQHMDVEESQCLRL
CPFLEDHKEDILCGPVWLASGLDLSGHAGMLTLTSPKLVKGMAGGKYRSFLIHVKAVNERGTEEICNGGMRPVVRLPSLKHQSNKGYSLA
SLLAKVAAGKEKSSNVKNENTSGTRKSENLRGCDLLQEVSVTIRRFKKTSISKERVQRCAMLQFSEFHEKLVNTLCRKTDDGQITEHAQS
LVLDTLCWLAGVHSNGPGSSKEGNENLLSKTRKFLSDIVRVCFFEAGRSIAHKCARFLALCISNGKCDPCQPAFGPVLLKALLDNMSFLP
AATTGGSVYWYFVLLNYVKDEDLAGCSTACASLLTAVSRQLQDRLTPMEALLQTRYGLYSSPFDPVLFDLEMSGSSCKNVYNSSIGVQSD
EIDLSDVLSGNGKVSSCTAAEGSFTSLTGLLEVEPLHFTCVSTSDGTRIERDDAMSSFGVTPAVGGLSSGTVGEASTALSSAAQVALQSL
SHAMASAEQQLQVLQEKQQQLLKLQQQKAKLEAKLHQTTAAAAAAASAVGPVHNSVPSNPVAAPGFFIHPSDVIPPTPKTTPLFMTPPLT
PPNEAVSVVINAELAQLFPGSVIDPPAVNLAAHNKNSNKSRMNPLGSGLALAISHASHFLQPPPHQSIIIERMHSGARRFVTLDFGRPIL
LTDVLIPTCGDLASLSIDIWTLGEEVDGRRLVVATDISTHSLILHDLIPPPVCRFMKITVIGRYGSTNARAKIPLGFYYGHTYILPWESE
LKLMHDPLKGEGESANQPEIDQHLAMMVALQEDIQCRYNLACHRLETLLQSIDLPPLNSANNAQYFLRKPDKAVEEDSRVFSAYQDCIQL
QLQLNLAHNAVQRLKVALGASRKMLSETSNPEDLIQTSSTEQLRTIIRYLLDTLLSLLHASNGHSVPAVLQSTFHAQACEELFKHLCISG
TPKIRLHTGLLLVQLCGGERWWGQFLSNVLQELYNSEQLLIFPQDRVFMLLSCIGQRSLSNSGVLESLLNLLDNLLSPLQPQLPMHRRTE
GVLDIPMISWVVMLVSRLLDYVATVEDEAAAAKKPLNGNQWSFINNNLHTQSLNRSSKGSSSLDRLYSRKIRKQLVHHKQQLNLLKAKQK
ALVEQMEKEKIQSNKGSSYKLLVEQAKLKQATSKHFKDLIRLRRTAEWSRSNLDTEVTTAKESPEIEPLPFTLAHERCISVVQKLVLFLL
SMDFTCHADLLLFVCKVLARIANATRPTIHLCEIVNEPQLERLLLLLVGTDFNRGDISWGGAWAQYSLTCMLQDILAGELLAPVAAEAME
EGTVGDDVGATAGDSDDSLQQSSVQLLETIDEPLTHDITGAPPLSSLEKDKEIDLELLQDLMEVDIDPLDIDLEKDPLAAKVFKPISSTW
YDYWGADYGTYNYNPYIGGLGIPVAKPPANTEKNGSQTVSVSVSQALDARLEVGLEQQAELMLKMMSTLEADSILQALTNTSPTLSQSPT
GTDDSLLGGLQAANQTSQLIIQLSSVPMLNVCFNKLFSMLQVHHVQLESLLQLWLTLSLNSSSTGNKENGADIFLYNANRIPVISLNQAS
ITSFLTVLAWYPNTLLRTWCLVLHSLTLMTNMQLNSGSSSAIGTQESTAHLLVSDPNLIHVLVKFLSGTSPHGTNQHSPQVGPTATQAMQ
EFLTRLQVHLSSTCPQIFSEFLLKLIHILSTERGAFQTGQGPLDAQVKLLEFTLEQNFEVVSVSTISAVIESVTFLVHHYITCSDKVMSR
SGSDSSVGARACFGGLFANLIRPGDAKAVCGEMTRDQLMFDLLKLVNILVQLPLSGNREYSARVSVTTNTTDSVSDEEKVSGGKDGNGSS
TSVQGSPAYVADLVLANQQIMSQILSALGLCNSSAMAMIIGASGLHLTKHENFHGGLDAISVGDGLFTILTTLSKKASTVHMMLQPILTY
MACGYMGRQGSLATCQLSEPLLWFILRVLDTSDALKAFHDMGGVQLICNNMVTSTRAIVNTARSMVSTIMKFLDSGPNKAVDSTLKTRIL
ASEPDNAEGIHNFAPLGTITSSSPTAQPAEVLLQATPPHRRARSAAWSYIFLPEEAWCDLTIHLPAAVLLKEIHIQPHLASLATCPSSVS
VEVSADGVNMLPLSTPVVTSGLTYIKIQLVKAEVASAVCLRLHRPRDASTLGLSQIKLLGLTAFGTTSSATVNNPFLPSEDQVSKTSIGW
LRLLHHCLTHISDLEGMMASAAAPTANLLQTCAALLMSPYCGMHSPNIEVVLVKIGLQSTRIGLKLIDILLRNCAASGSDPTDLNSPLLF
GRLNGLSSDSTIDILYQLGTTQDPGTKDRIQALLKWVSDSARVAAMKRSGRMNYMCPNSSTVEYGLLMPSPSHLHCVAAILWHSYELLVE
YDLPALLDQELFELLFNWSMSLPCNMVLKKAVDSLLCSMCHVHPNYFSLLMGWMGITPPPVQCHHRLSMTDDSKKQDLSSSLTDDSKNAQ
APLALTESHLATLASSSQSPEAIKQLLDSGLPSLLVRSLASFCFSHISSSESIAQSIDISQDKLRRHHVPQQCNKMPITADLVAPILRFL
TEVGNSHIMKDWLGGSEVNPLWTALLFLLCHSGSTSGSHNLGAQQTSARSASLSSAATTGLTTQQRTAIENATVAFFLQCISCHPNNQKL
MAQVLCELFQTSPQRGNLPTSGNISGFIRRLFLQLMLEDEKVTMFLQSPCPLYKGRINATSHVIQHPMYGAGHKFRTLHLPVSTTLSDVL
DRVSDTPSITAKLISEQKDDKEKKNHEEKEKVKAENGFQDNYSVVVASGLKSQSKRAVSATPPRPPSRRGRTIPDKIGSTSGAEAANKII
TVPVFHLFHKLLAGQPLPAEMTLAQLLTLLYDRKLPQGYRSIDLTVKLGSRVITDPSLSKTDSYKRLHPEKDHGDLLASCPEDEALTPGD
ECMDGILDESLLETCPIQSPLQVFAGMGGLALIAERLPMLYPEVIQQVSAPVVTSTTQEKPKDSDQFEWVTIEQSGELVYEAPETVAAEP
PPIKSAVQTMSPIPAHSLAAFGLFLRLPGYAEVLLKERKHAQCLLRLVLGVTDDGEGSHILQSPSANVLPTLPFHVLRSLFSTTPLTTDD
GVLLRRMALEIGALHLILVCLSALSHHSPRVPNSSVNQTEPQVSSSHNPTSTEEQQLYWAKGTGFGTGSTASGWDVEQALTKQRLEEEHV
TCLLQVLASYINPVSSAVNGEAQSSHETRGQNSNALPSVLLELLSQSCLIPAMSSYLRNDSELKPEQVKNMSASEMRNIRLSDFTESLKK

--------------------------------------------------------------
>9768_9768_4_BIRC6-SPAST_BIRC6_chr2_32774548_ENST00000421745_SPAST_chr2_32372287_ENST00000345662_length(transcript)=16582nt_BP=13278nt
TCCCTGCTTCTCCCCCTCTCCCGTCAGCCTCCCTCCGAGTTTGGCCCCTCCGGCCGGGCGATCGACGTTCCGCGTGCGTGCGGGCGCCTG
ACTTCACTTCCGGCTAACGCGCTCGGCTTGCCCCCTGGCCCCGGATGGTGACTGGTGGTGGTGCTGCACCTCCCGGGACTGTCACTGAGC
CGCTTCCCAGTGTGATTGTGCTGAGCGCAGGCCGGAAGATGGCGGCTGCGGCTGCGGCGGCCTCGGGCCCCGGCTGCTCCTCGGCGGCGG
GGGCGGGGGCGGCCGGGGTCTCAGAGTGGCTGGTGCTGCGGGACGGCTGCATGCACTGCGACGCCGACGGGCTGCACAGCCTGTCCTACC
ACCCTGCGCTCAACGCCATCCTGGCCGTCACTAGCCGCGGGACCATCAAAGTCATCGACGGCACCTCGGGGGCCACACTGCAGGCCTCCG
CGCTCAGTGCTAAACCAGGTGGACAGGTGAAATGTCAGTATATCTCTGCTGTGGATAAAGTTATATTTGTGGATGATTATGCAGTAGGGT
GTAGGAAGGACCTTAATGGAATCTTGTTGTTAGACACTGCTCTGCAAACTCCAGTTTCAAAGCAGGATGATGTGGTTCAGCTTGAATTAC
CCGTTACAGAGGCACAGCAGCTCTTATCAGCATGTTTAGAAAAGGTAGATATTTCTAGTACAGAGGGTTATGATTTGTTCATCACACAGC
TCAAAGATGGTTTAAAAAATACATCTCATGAGACTGCAGCAAACCACAAAGTTGCTAAGTGGGCCACAGTTACATTTCATCTTCCTCATC
ATGTGTTGAAGTCCATTGCCAGTGCCATTGTAAATGAACTCAAGAAAATAAATCAAAATGTTGCTGCCTTACCTGTGGCGTCCTCAGTGA
TGGACAGATTGTCTTACCTCTTACCTAGTGCACGTCCAGAACTCGGAGTGGGGCCAGGCCGTTCTGTAGACAGATCACTGATGTATAGTG
AAGCTAACAGACGGGAGACATTTACCTCATGGCCTCATGTAGGCTATAGGTGGGCACAACCAGATCCCATGGCTCAAGCTGGATTTTATC
ATCAGCCTGCCTCATCTGGAGATGATAGAGCCATGTGTTTTACTTGTAGTGTATGCCTCGTTTGTTGGGAACCTACTGATGAACCTTGGT
CTGAACACGAAAGACATTCCCCAAACTGCCCATTTGTGAAAGGTGAGCACACACAGAATGTGCCATTGTCAGTCACTCTTGCAACAAGTC
CTGCACAGTTTCCTTGTACGGATGGAACTGACAGAATATCTTGCTTTGGGTCGGGGAGCTGCCCTCATTTTCTAGCTGCTGCAACTAAAC
GAGGAAAGATCTGCATATGGGATGTTTCCAAACTTATGAAGGTGCACTTAAAGTTTGAAATTAATGCCTATGATCCAGCAATTGTACAAC
AGCTTATTCTATCAGGAGACCCAAGCTCAGGAGTTGATTCAAGGAGACCAACTTTGGCGTGGCTGGAGGACTCCTCTAGTTGCTCAGATA
TACCAAAATTGGAAGGAGATAGTGATGATTTACTGGAGGATTCAGACAGTGAAGAGCATTCCAGATCAGATTCTGTGACAGGGCATACAT
CACAGAAGGAAGCCATGGAAGTAAGCCTTGATATAACAGCACTCAGCATTCTCCAACAGCCAGAAAAACTTCAGTGGGAGATTGTTGCAA
ATGTGCTTGAAGATACTGTTAAGGATCTTGAAGAACTTGGGGCAAATCCTTGTTTAACAAACTCTAAGAGTGAAAAGACAAAGGAAAAGC
ACCAGGAGCAACACAACATTCCTTTTCCATGTTTATTAGCTGGAGGTTTATTAACATATAAATCTCCTGCTACCTCACCCATTAGTAGTA
ATTCTCACAGGTCACTGGATGGTTTAAGCAGAACTCAGGGTGAAAGTATATCAGAACAAGGGTCAACTGACAATGAATCCTGCACTAATT
CAGAACTAAATTCTCCTCTGGTAAGGAGGACTTTACCGGTTTTGCTTCTTTATAGCATCAAGGAATCTGATGAGAAAGCAGGAAAGATCT
TTTCACAGATGAACAATATTATGAGTAAAAGTTTGCATGATGATGGTTTTACTGTTCCACAGATTATTGAAATGGAGCTGGATAGTCAGG
AGCAGTTGTTATTGCAGGATCCTCCTGTGACTTACATTCAGCAATTTGCAGATGCAGCAGCCAACCTTACCTCTCCGGATTCTGAGAAGT
GGAACTCTGTGTTTCCCAAGCCTGGGACTTTGGTTCAGTGCTTGAGGCTGCCAAAGTTTGCAGAGGAGGAGAATCTTTGTATAGACTCAA
TAACTCCTTGTGCTGACGGAATTCATTTGTTGGTAGGACTGCGGACATGCCCTGTTGAATCCTTGAGTGCAATAAATCAAGTAGAGGCCT
TGAATAATTTAAATAAATTAAACTCTGCACTATGTAATAGACGGAAAGGTGAGCTGGAATCAAATCTTGCTGTAGTGAATGGTGCAAATA
TTAGTGTAATCCAACATGAATCACCAGCAGATGTACAGACTCCTTTAATAATTCAGCCTGAGCAGAGGAATGTTAGTGGTGGATATTTAG
TGCTTTATAAAATGAATTATGCCACTCGGATAGTGACTTTAGAAGAGGAGCCAATAAAAATACAACATATCAAAGATCCCCAGGACACAA
TTACCTCGCTCATTTTGCTTCCACCCGATATATTGGATAATCGAGAGGATGACTGTGAGGAACCTATTGAGGACATGCAGTTAACCTCAA
AGAATGGTTTTGAGAGAGAAAAAACGTCTGACATTTCTACTCTTGGACACCTGGTAATAACCACTCAGGGAGGATATGTAAAAATACTAG
ATCTTTCAAACTTTGAAATTTTGGCCAAAGTGGAGCCTCCCAAAAAGGAGGGCACTGAGGAACAGGACACATTTGTTTCTGTGATTTACT
GTTCTGGCACAGACAGGCTGTGTGCATGCACCAAAGGTGGTGAGCTTCATTTTCTCCAAATTGGAGGAACCTGTGATGATATTGATGAAG
CTGATATACTAGTGGATGGATCTCTTTCTAAAGGAATAGAACCATCTTCAGAAGGTTCCAAACCTTTATCAAATCCTTCAAGTCCTGGCA
TTTCAGGAGTTGATTTATTGGTGGACCAGCCATTCACCCTTGAAATCTTGACATCCCTAGTGGAGCTAACCCGCTTTGAGACTTTGACTC
CAAGGTTTTCAGCGACTGTTCCTCCATGCTGGGTAGAAGTTCAACAAGAACAGCAGCAAAGGAGGCATCCTCAACATTTGCATCAGCAAC
ACCATGGTGATGCTGCTCAGCATACTCGAACTTGGAAACTACAGACCGACAGCAACAGCTGGGATGAACATGTATTTGAATTAGTACTAC
CTAAAGCTTGTATGGTTGGACATGTGGACTTCAAATTCGTTTTGAACTCAAACATCACCAATATTCCACAGATACAAGTGACACTGCTGA
AAAATAAAGCTCCAGGATTAGGGAAAGTCAATGCTCTTAACATTGAAGTGGAACAAAATGGGAAACCGTCCCTGGTTGATTTGAATGAAG
AAATGCAGCACATGGATGTAGAGGAATCACAGTGTCTTAGATTATGTCCATTTTTGGAGGATCATAAAGAAGACATTCTATGTGGGCCAG
TATGGCTTGCTAGTGGCCTTGATCTATCAGGGCATGCTGGAATGTTGACGTTAACAAGCCCCAAACTTGTTAAAGGTATGGCAGGAGGAA
AATATCGTTCGTTTTTAATCCATGTCAAGGCAGTGAATGAAAGAGGAACAGAAGAGATTTGTAATGGTGGTATGCGTCCTGTAGTAAGGC
TTCCATCCCTAAAACACCAGAGTAACAAGGGTTATTCACTTGCTTCACTTTTGGCTAAAGTTGCAGCAGGCAAGGAAAAATCATCTAATG
TTAAGAATGAAAATACAAGTGGCACCCGTAAATCTGAAAACCTCCGGGGCTGTGATTTACTTCAAGAGGTCTCAGTCACCATTCGAAGAT
TTAAGAAAACCTCAATTTCTAAGGAAAGAGTGCAACGATGTGCCATGTTACAGTTTTCAGAATTTCATGAGAAGCTTGTTAATACTCTTT
GCAGAAAAACAGATGATGGCCAGATCACAGAACATGCCCAGAGCCTTGTGTTGGATACTCTCTGTTGGTTAGCTGGAGTTCATTCAAATG
GACCCGGAAGCTCAAAGGAAGGAAATGAGAACCTACTTTCAAAAACACGAAAATTTCTGTCAGACATCGTACGTGTTTGCTTCTTTGAGG
CAGGACGAAGTATAGCCCATAAGTGTGCCCGATTTCTAGCCTTGTGCATTAGTAATGGCAAATGTGACCCATGTCAACCAGCATTTGGAC
CTGTTCTGTTGAAGGCTTTACTTGATAATATGTCATTTTTACCTGCAGCAACAACTGGTGGTTCTGTCTATTGGTATTTTGTCTTACTGA
ATTATGTGAAAGATGAAGATCTGGCTGGATGCAGTACAGCTTGTGCATCTTTGCTTACTGCAGTGTCCAGACAGTTACAGGACAGGCTAA
CACCAATGGAGGCTTTACTTCAGACAAGATATGGATTATATAGCTCACCATTTGATCCAGTCCTCTTTGATTTGGAGATGAGTGGCTCTT
CTTGTAAAAATGTTTATAACAGCAGCATTGGTGTCCAGTCAGATGAAATTGATTTATCAGATGTCCTTTCAGGAAATGGAAAGGTCAGTA
GTTGCACAGCTGCTGAGGGTAGTTTCACATCTCTCACTGGACTTTTGGAAGTTGAACCTCTGCACTTTACTTGTGTGTCAACTAGTGATG
GAACCAGAATAGAAAGGGATGATGCAATGAGTTCCTTCGGGGTTACTCCTGCAGTAGGTGGACTATCATCTGGGACAGTTGGGGAAGCCT
CGACAGCCCTGAGTTCAGCAGCCCAGGTAGCTTTGCAGTCTCTCTCTCATGCAATGGCTTCAGCCGAGCAACAGCTACAGGTGCTGCAAG
AGAAACAGCAGCAGCTTTTGAAGCTTCAGCAACAGAAAGCAAAGCTGGAAGCCAAGTTACATCAGACAACAGCTGCAGCAGCTGCAGCAG
CATCAGCAGTAGGTCCTGTTCACAACTCTGTGCCTTCCAACCCAGTGGCTGCCCCTGGATTCTTCATTCATCCATCTGATGTTATTCCAC
CCACTCCAAAAACAACACCTCTTTTTATGACTCCACCACTCACTCCACCCAATGAAGCAGTTTCCGTTGTGATTAATGCCGAACTTGCAC
AGCTTTTCCCAGGCTCAGTCATTGATCCCCCAGCAGTCAATCTTGCTGCACATAACAAAAATTCCAACAAGTCCAGAATGAATCCACTTG
GTTCTGGTCTAGCCCTTGCAATTTCTCATGCTTCACATTTTCTTCAACCTCCGCCTCACCAGTCCATTATTATAGAGCGAATGCATTCAG
GAGCAAGAAGATTTGTGACCTTGGATTTTGGGAGGCCTATATTGTTGACTGATGTATTGATTCCCACTTGTGGAGACTTGGCCTCTTTGT
CAATTGACATTTGGACATTAGGAGAAGAGGTGGATGGAAGGCGGTTGGTAGTGGCAACTGATATAAGCACTCATTCACTAATTCTTCATG
ACTTAATACCACCTCCCGTGTGCAGATTCATGAAGATCACTGTTATTGGACGTTACGGGAGTACAAATGCCAGAGCCAAAATCCCATTAG
GATTTTACTATGGTCATACCTACATCTTGCCTTGGGAAAGTGAACTGAAGTTAATGCATGATCCTCTAAAGGGAGAGGGAGAATCTGCAA
ACCAGCCAGAAATTGACCAGCATTTAGCAATGATGGTTGCTTTGCAGGAGGATATACAGTGCAGGTATAACTTGGCTTGTCATCGTCTGG
AAACCCTTTTGCAAAGTATTGATCTTCCTCCTCTAAACAGTGCTAACAATGCACAGTACTTTTTACGAAAACCAGATAAGGCAGTTGAGG
AAGACAGTAGGGTTTTTTCTGCTTATCAAGATTGTATTCAGCTACAGCTTCAACTAAATTTGGCTCATAATGCAGTGCAGAGGCTCAAAG
TGGCGCTAGGTGCAAGCCGGAAGATGTTGAGTGAAACATCAAATCCAGAAGATTTAATTCAGACATCTTCCACAGAGCAGTTACGTACTA
TCATCAGATATTTACTGGACACTTTGCTCAGCCTGCTTCATGCTTCTAATGGACACTCTGTTCCTGCAGTTTTGCAGAGCACATTTCATG
CCCAGGCCTGTGAAGAGCTCTTTAAACACTTGTGCATCAGTGGAACCCCAAAGATACGGTTACATACTGGTCTTCTTCTTGTTCAACTGT
GTGGTGGTGAAAGGTGGTGGGGTCAATTTCTTTCTAATGTCCTTCAGGAATTGTACAATTCGGAACAGCTTCTCATCTTTCCACAGGATA
GGGTCTTCATGTTACTTTCCTGCATTGGTCAAAGATCACTTAGTAATAGTGGAGTATTAGAAAGCTTACTTAATCTCTTGGATAATTTAT
TGTCACCTCTTCAGCCACAGTTACCCATGCATAGGAGGACAGAAGGAGTACTAGATATTCCCATGATCAGTTGGGTTGTTATGCTGGTGT
CCAGGTTGCTGGATTATGTGGCAACTGTTGAAGATGAAGCAGCAGCTGCAAAGAAACCTTTGAATGGTAATCAGTGGAGTTTTATTAACA
ATAATCTACACACTCAGAGCTTAAATAGATCTTCTAAAGGCAGCAGTAGCCTTGATAGATTATATTCCAGAAAAATCAGAAAGCAGCTTG
TTCATCATAAACAGCAACTTAACCTACTAAAAGCAAAGCAGAAGGCATTGGTAGAACAGATGGAAAAAGAAAAAATACAAAGTAACAAAG
GATCATCATATAAACTCCTGGTAGAACAAGCAAAACTAAAGCAGGCCACTTCAAAGCACTTTAAGGATTTAATTCGTTTACGTCGGACAG
CAGAATGGTCCCGTTCTAATTTAGACACAGAAGTTACAACAGCAAAAGAAAGTCCTGAGATAGAACCACTTCCATTTACTCTGGCCCATG
AGCGTTGTATCTCAGTAGTCCAGAAACTTGTTCTGTTTCTTCTCTCCATGGACTTTACATGTCATGCAGATCTCTTATTGTTTGTTTGTA
AGGTTCTTGCACGCATTGCAAATGCCACGAGGCCAACTATTCATCTGTGTGAGATTGTGAACGAACCCCAGCTGGAAAGACTGCTGTTAC
TTTTGGTTGGAACTGACTTCAATAGAGGAGATATATCTTGGGGTGGTGCTTGGGCTCAGTATTCCTTAACTTGCATGCTACAAGATATTT
TAGCAGGAGAATTACTGGCTCCAGTAGCCGCAGAAGCCATGGAGGAAGGAACAGTGGGTGATGATGTAGGTGCGACAGCTGGTGACTCTG
ATGACTCCCTTCAACAGTCCTCAGTTCAGTTGCTGGAAACTATAGATGAACCTTTGACACATGACATAACAGGTGCACCTCCTCTGTCCT
CTTTGGAAAAAGATAAAGAAATTGACCTTGAGTTACTTCAGGATCTAATGGAAGTTGACATTGATCCTTTAGATATTGATTTGGAAAAGG
ACCCTCTTGCAGCCAAGGTTTTTAAGCCAATAAGCAGTACATGGTATGATTATTGGGGTGCTGATTATGGGACCTACAATTACAACCCTT
ACATTGGAGGTCTGGGAATTCCTGTAGCAAAGCCACCAGCAAACACGGAGAAGAACGGATCACAGACAGTTAGCGTTTCAGTCTCTCAGG
CCCTGGATGCTCGCCTAGAAGTTGGACTTGAACAGCAAGCAGAACTGATGTTGAAAATGATGTCTACTCTGGAGGCAGATTCCATTTTAC
AGGCATTAACAAATACATCTCCTACATTATCACAGTCTCCCACTGGAACAGATGATTCACTTCTAGGGGGTTTACAAGCAGCAAACCAAA
CCAGCCAGCTTATTATACAGTTATCATCTGTCCCAATGTTAAATGTTTGTTTCAACAAACTTTTTTCCATGCTTCAAGTCCATCATGTTC
AGTTGGAGTCACTTCTCCAATTGTGGCTCACACTGAGCCTGAATTCTAGTTCAACTGGAAACAAAGAAAATGGAGCAGACATATTTTTAT
ATAATGCTAATAGGATACCTGTTATTTCATTAAATCAAGCATCAATAACTAGCTTTCTCACAGTGTTAGCTTGGTATCCCAATACTTTGC
TCCGGACATGGTGCCTTGTGCTTCATAGCCTAACACTCATGACAAACATGCAGCTTAATTCTGGTTCCAGCAGTGCCATTGGAACTCAGG
AGAGTACTGCTCATTTGTTGGTTTCAGATCCAAACCTAATTCATGTATTAGTGAAATTTCTTTCTGGCACCAGTCCACATGGAACAAATC
AACACAGTCCACAGGTTGGTCCTACAGCTACACAAGCTATGCAAGAATTTCTTACTCGATTACAAGTGCATCTTTCTTCAACATGTCCTC
AGATATTCAGTGAATTTTTGCTCAAGCTAATTCATATACTTTCAACTGAAAGGGGTGCTTTCCAGACAGGCCAAGGACCTCTCGATGCCC
AAGTGAAGCTCTTAGAATTCACTCTGGAGCAGAATTTTGAAGTCGTTTCAGTTAGTACTATTTCTGCCGTGATAGAATCGGTTACATTTT
TAGTGCACCACTATATCACTTGCTCAGACAAAGTAATGTCAAGAAGTGGATCAGATAGCTCCGTGGGTGCTCGAGCATGCTTTGGGGGAC
TCTTTGCCAATCTTATTCGTCCGGGTGATGCAAAAGCAGTTTGTGGCGAAATGACAAGAGATCAACTCATGTTTGATTTGTTAAAACTTG
TTAACATTTTAGTGCAGCTGCCTCTTTCAGGCAATAGGGAATACAGTGCAAGAGTGTCTGTGACCACAAATACAACAGATAGTGTTTCAG
ATGAAGAAAAAGTCTCAGGAGGCAAAGATGGCAATGGAAGCAGTACCAGTGTTCAAGGATCGCCTGCATATGTTGCTGACTTAGTCTTAG
CCAACCAACAAATTATGAGCCAGATTTTGTCTGCTCTGGGCCTGTGTAATAGCAGTGCCATGGCAATGATAATTGGAGCAAGTGGATTAC
ATCTCACTAAACATGAAAACTTTCATGGTGGGTTGGATGCCATATCAGTTGGGGATGGATTATTTACCATACTGACAACCCTTAGTAAAA
AAGCTTCTACAGTCCACATGATGCTGCAGCCAATTTTAACATACATGGCCTGTGGATATATGGGCAGACAAGGCTCTCTTGCTACTTGCC
AGTTATCTGAGCCATTATTGTGGTTCATTTTGAGAGTATTGGATACTAGTGATGCCTTGAAAGCATTTCATGATATGGGTGGTGTTCAGC
TCATATGCAATAATATGGTTACTAGTACAAGGGCTATTGTGAACACTGCAAGAAGTATGGTATCAACTATTATGAAATTTCTTGACTCTG
GTCCAAATAAAGCTGTTGACAGCACATTGAAAACAAGAATACTAGCTTCTGAGCCTGACAATGCTGAAGGGATTCATAACTTTGCACCCC
TCGGTACAATCACATCTAGCAGTCCTACTGCCCAACCAGCTGAAGTGCTATTGCAGGCCACACCTCCTCACAGAAGAGCTCGCTCTGCTG
CTTGGTCCTACATCTTTCTTCCAGAGGAGGCTTGGTGTGACCTTACCATTCACCTTCCTGCAGCAGTGCTGCTTAAGGAGATACATATCC
AGCCTCATCTTGCATCTCTTGCAACCTGCCCTTCCTCAGTGTCTGTTGAAGTAAGTGCAGATGGGGTAAATATGCTACCTTTGTCCACTC
CTGTTGTCACAAGTGGCCTCACCTACATAAAAATTCAGCTTGTAAAAGCCGAAGTAGCTTCTGCTGTCTGCCTTAGACTACATCGTCCAC
GGGATGCCAGCACATTAGGCCTTTCACAAATTAAATTATTGGGGCTCACTGCTTTTGGTACCACCTCTTCTGCAACAGTTAATAATCCAT
TCCTTCCATCTGAAGATCAGGTATCCAAAACAAGTATTGGATGGTTACGGTTATTACATCATTGCCTTACTCACATAAGTGATCTAGAAG
GAATGATGGCAAGTGCAGCTGCACCTACTGCTAATCTGCTGCAGACTTGTGCGGCCTTATTGATGTCACCTTACTGTGGAATGCATTCAC
CCAACATCGAGGTTGTGCTTGTAAAGATAGGACTGCAGTCTACTAGAATTGGCCTGAAGCTCATAGACATTCTCCTGAGAAATTGTGCAG
CATCAGGCAGTGATCCTACAGATTTGAATAGTCCTTTACTTTTTGGAAGACTAAATGGACTCTCTTCTGACTCTACGATAGATATTCTTT
ACCAGCTTGGAACAACTCAGGATCCTGGTACAAAAGACAGAATTCAGGCCTTGTTAAAATGGGTTAGTGATTCTGCAAGAGTGGCTGCTA
TGAAGAGAAGTGGCAGGATGAACTACATGTGTCCTAACTCCTCAACAGTAGAGTATGGTCTTCTGATGCCATCTCCTTCTCATTTGCACT
GTGTAGCAGCCATTCTGTGGCATAGTTATGAGCTGCTTGTAGAATATGACTTACCAGCACTCCTGGACCAAGAGCTCTTTGAGTTACTTT
TTAATTGGTCCATGTCTCTTCCCTGCAATATGGTTTTGAAGAAAGCTGTTGACAGTCTACTTTGCTCAATGTGTCACGTACACCCAAACT
ATTTTTCTTTGCTCATGGGCTGGATGGGAATTACCCCTCCTCCAGTGCAATGTCATCATAGACTGTCCATGACAGATGATAGCAAAAAGC
AGGATCTTAGTTCATCTTTAACAGATGACTCTAAAAATGCACAAGCACCTCTCGCATTAACTGAATCACATTTGGCTACCCTTGCTTCCT
CTTCTCAATCTCCTGAAGCTATTAAACAATTACTAGACTCAGGTTTGCCTTCTCTTCTTGTGAGGAGTCTGGCTAGTTTCTGCTTTAGCC
ACATTTCTAGCTCAGAAAGCATTGCCCAGTCAATAGATATTTCCCAGGACAAACTCAGGCGCCATCATGTCCCACAACAATGTAATAAGA
TGCCTATCACAGCCGACCTAGTTGCTCCTATTCTTAGGTTTTTGACAGAAGTTGGCAATAGCCATATTATGAAAGATTGGCTTGGTGGTT
CTGAAGTCAATCCACTATGGACAGCACTTCTGTTTTTATTGTGTCACTCTGGGTCCACTTCTGGAAGCCATAATTTAGGTGCACAACAGA
CCAGTGCAAGATCAGCTTCTCTTTCTTCAGCTGCTACAACAGGACTGACTACTCAACAGCGCACAGCAATTGAGAATGCAACTGTTGCGT
TCTTTCTACAGTGCATTTCATGCCATCCTAATAATCAAAAGCTGATGGCACAGGTTCTTTGTGAACTATTTCAGACATCTCCTCAAAGAG
GGAACCTTCCAACATCTGGGAACATTTCAGGGTTTATACGAAGATTATTTTTACAGTTGATGCTGGAAGATGAGAAAGTGACAATGTTTC
TTCAGTCTCCATGTCCACTGTACAAAGGTAGAATTAATGCTACTAGCCACGTCATCCAGCATCCAATGTATGGAGCAGGCCACAAATTCC
GTACTCTTCATTTGCCAGTCTCAACAACATTATCAGATGTTCTTGACAGAGTGTCAGATACTCCAAGTATCACAGCTAAATTAATTAGTG
AACAAAAAGATGACAAAGAAAAGAAAAACCATGAAGAGAAAGAAAAAGTTAAAGCGGAAAATGGATTTCAAGACAATTACAGTGTTGTTG
TTGCCTCTGGGCTGAAGTCTCAATCTAAACGTGCTGTGTCAGCTACACCACCTCGCCCACCATCCAGGAGGGGGAGGACAATACCTGATA
AAATAGGAAGTACTTCAGGAGCAGAGGCTGCCAACAAAATAATTACTGTCCCAGTGTTTCACCTGTTTCACAAACTCTTGGCAGGCCAGC
CATTGCCAGCTGAAATGACACTTGCCCAGCTTTTAACTCTCCTATATGACCGAAAACTTCCTCAGGGTTACCGCTCAATAGATCTGACTG
TTAAATTGGGATCAAGAGTTATAACAGACCCCAGTCTATCAAAAACAGATTCTTATAAAAGACTACACCCTGAAAAAGATCATGGAGACT
TACTTGCTAGCTGTCCAGAAGATGAGGCTCTCACTCCAGGTGATGAATGCATGGATGGGATACTGGATGAATCTTTGCTTGAAACCTGTC
CAATTCAGTCACCATTACAAGTTTTTGCAGGAATGGGTGGACTGGCTCTTATTGCTGAAAGACTACCCATGCTATATCCAGAAGTAATTC
AACAGGTGAGTGCTCCAGTTGTAACATCTACCACTCAGGAAAAGCCGAAGGATAGCGATCAGTTTGAATGGGTGACCATTGAACAGTCAG
GGGAGTTAGTTTATGAAGCACCAGAAACTGTTGCGGCTGAACCTCCACCTATCAAGTCAGCAGTACAGACCATGTCTCCCATACCTGCCC
ATTCTTTGGCTGCTTTTGGATTATTTCTTCGTCTTCCGGGCTATGCGGAAGTGCTACTGAAAGAGAGAAAACATGCCCAGTGCCTTCTTC
GATTGGTATTGGGAGTGACAGATGATGGAGAAGGAAGTCATATTCTTCAATCTCCATCAGCCAATGTGCTTCCAACCCTTCCTTTCCACG
TCCTTCGTAGCTTGTTTAGCACTACACCTTTGACAACTGATGATGGTGTACTTCTAAGGCGGATGGCATTGGAAATTGGAGCCTTACACC
TCATTCTTGTCTGTCTCTCTGCTTTGAGCCACCATTCCCCACGAGTTCCAAACTCTAGCGTGAATCAAACTGAGCCACAGGTGTCAAGCT
CTCATAACCCTACATCAACAGAAGAACAACAGTTATATTGGGCCAAAGGGACTGGCTTTGGAACAGGCTCTACAGCTTCTGGGTGGGATG
TGGAACAAGCCTTAACTAAGCAAAGGCTGGAAGAGGAACATGTTACCTGCCTTCTGCAGGTTCTTGCCAGTTACATAAATCCCGTCAGTA
GTGCGGTAAATGGAGAAGCTCAGTCATCTCATGAGACTAGAGGGCAGAACAGTAATGCCCTTCCTTCTGTACTTCTCGAGCTTCTCAGTC
AGTCCTGCCTCATCCCAGCCATGTCATCTTATCTACGAAATGATTCAGAACTAAAACCAGAACAGGTGAAGAATATGTCTGCCAGTGAGA
TGAGAAATATTCGATTATCTGACTTCACTGAATCCTTGAAAAAAATAAAACGCAGCGTCAGCCCTCAAACTTTAGAAGCGTACATACGTT
GGAACAAGGACTTTGGAGATACCACTGTTTAAGGAAATACCTTTGTAAACCTGCAGAACATTTTACTTAAAAGAGGAAACACAAGATCTT
CAATGAACGTCATCGGCTACAGAAACAGCCTAAGTTTACAGGACTTTTTAGAGTCTTACATATTTGTGCACCAAACTTGAAGATGAACCA
GAAAACAGACTTAAACAAAATATACAATGCAAATGTAATTTTTTGTTGTTTAAGGCCTTGCCTTGATGGTCACAGTTATCCCAATGGACA
CTAAGTTAGAGCACAACAAAACCTGATTCTGGTCTTCTTTACCAATATAATCATAATGTAAATAATAATTTGTATATTGTGTTGCAGATG
AAAGTATTCCAGGAACAGTGAATGGTAGAAGACACAAGAACATTTGTTTGTTTGTCTTCTGATGTTTTTTCTTAAAATAGTAATTTCTCC
TACTTTTCTTTTCTACTGTTGTCTTAACTACAGGTGATTGGAATGCCAAACACTCTTAAGTTTATTTTCTTTTTTCGTTTTATAAATTCA
GTGTGCCAAATGAAACTTTTTTCCTAAGTAACTGTAATAGGAAAAAGTTTATTTTGAGAGTTTCTTCTTCATAAATCTACAGACATTAAA
CAATTGTTGTGTTCTTTTTACCTTTTATTTTTCTATTACCTTGCTACCAAACAGTTTAGATAGCAATATAATAGCAAAAAAGCAAATATG
GTAAAATAGAGAAGGTTTGAAGGTTTGAGTTACTCTGTCATATAACATGTAGATCAGTCTTCATGTGACCTGCAGTATTTTTTTTTCTAA
TGTATTTGTCAGAAATCTGTTGTAGACTGTTAACTTCTTCCTGATGGAATTTATTTTCTGCAAGAATTATTCTGATATTTAAGAGAGCCA
ATTTTAACTGCTGTGAAAATGTTTCCAGTGCAAGAGAAGGGAAATACTAGGAACTAAGACATTTCTAATTTATTGCTTATTACTTTCTTA
ATTTTACAGGATAATTATAAGCAAGTGGAACTACCATCTTTTATTCTTAATAATTATTAATCCCTTCAATGAAACTTTAAAAAAACTGAA
TTTTTATACATGGCATACATTTTTCTAGTTCCTTCTGCTTGCTTTATTAACTCAAAAGTTCTAGTTCTAGTCTGTTGATCTGCCTTTTGT
TCTCCCAAAATGTACAGTAATTCCATTTGTTTGTATAAATATGCCTGGATTTTCATTATAAAAATGTCATTGTAGGGAGTAGAGACTCAT
ATCATGGCCTTTTAAATATTGTAATAAAGGCAAATAGATATTTGCCCTTAGTTTACTGGTTAAAAGTTTGTTTACAGAACTTTTCTCTGG
TGCTTAAATGATGCTATGTAAAATGTCATGAGTGGAAAGAATATTTGTAGTAGTAACAAGAATTTTTCATTTAGGAAAGATTTCTTAGGT
TTTGAAAGAATACATTAAAATAAAAAACTTGCCCCTACTAGGTAAGAACTTTATAATGAAGACATACATTCTTCTTAATTTTACTCTTGC
TCTTGTTAAAGATTTGTTTGAATATAGAAGATGCATGATTTCTGGGTTTTTTTTTTTTTTTGAGACAGAGTTTCGCTCTTGTTGCCCAGG
CTGGAGTGCAATGGCGCAATCTCGACTCACCACAACCTCCGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGG
ATTACAGGCATGCGCCACTACCCCAGCTAATTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTGGTCAGGCTGGTCTTGAACTCCT
GACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATAAGCCACTGCGCCCAGCCAGAAGATGCATGATTTCT
TAGGATCATATGCTGTTTGTAGCCATAAGGTAAATCATGTCTCTTCCAATCATGACTTTGGAACTCCCTGAATAATAAAAATGAGAGTTG
AGATAAATAGGGGAAAAAAAATTTTTTTCAAGCCAGAGCTATGCATATGTTAGGTGATGGGTAGTATCCCTTTAAGGTCTCAAACATTAC
AACATCAATTATGAAATACTGATAACGAAAGGTAGTAATGAAATATATATGATGAAAAGAATTGAGAAGTTCTAAATTAAGACATTTCAG
TTAAGCTCATAAAATTTCATTGTTTTCATTTAAAAGATTAACGTTATTGATACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGG
TTCCAGCTCAACTTTTAATATATTGTCTCCTTTAAAACTATCATGGTTATAATTCTATTGGGAAAGACTTTTAGATAACAAAGATTTCAA
ATGTTAAAAGAGATAAAAGTCAGGTTAATACTATCTTAAACACTGAGTCAGAAAATCATTACTGTATAGAAGTTGCTTTCCTGATCAAGT
CTGAACTTCAGCTAGTGCTAGAGAACTATTTTCTATGACTTAACTCTAACCAAGTTTTATTTTAAGCTGTTTCTTTGATAGAAGGGCCAT
GAAAATAGAGTAATGATATAGTAGGAGATAAGGGATTGGTTTGGTCTTTTTCAATAAAGATAGAAGTTGCTGAAGTTTTCTGAATTAATA
ATGACTTAGATTGTGACCTTTTAGATTCGGTGTTGAGCTCTGTGTTGTATTACTTCCTAAAAGATAATGCTTAAACATTAAGCATTAGTG
TGCTCTTCATGTTAATATGGCAGAGTTTTGTAAACTAAATTAAAACTTACTGATATATTGGACTTTGAGCCAAGGGAAAGAATGAGTACT
ATCTTTCCAGATATCTTAAGGGTAAAAGCTTATTCTAAGACAGTCTGTCCATTGAGAATATTAGATTTCTGACTTGCAAATATGTTTGTA
CTCCAGAAGAATTAGAGGAAAAGCAGATACTAGAATTCTAATTTAATTACATATACAGCCGTCTTTGTTTATAGTGTAGAATTCTTTATA
TTTTGTACAAAAACTAATTCTTTTGGTAAAATGAACCATTTACAGTTCGGTTTTGGACTCTGAGTCAAAGGATTTTCCTTTAAATGCTTG
TCTCAATTTTAGTCTGGTCTTTTGTACTTTTCTTCAGAAGAAATGAATTAAAGGGTACAGTTGCATAAAGTGGGTTTTTATCCTAATGTA

>9768_9768_4_BIRC6-SPAST_BIRC6_chr2_32774548_ENST00000421745_SPAST_chr2_32372287_ENST00000345662_length(amino acids)=4435AA_BP=4381
MVTGGGAAPPGTVTEPLPSVIVLSAGRKMAAAAAAASGPGCSSAAGAGAAGVSEWLVLRDGCMHCDADGLHSLSYHPALNAILAVTSRGT
IKVIDGTSGATLQASALSAKPGGQVKCQYISAVDKVIFVDDYAVGCRKDLNGILLLDTALQTPVSKQDDVVQLELPVTEAQQLLSACLEK
VDISSTEGYDLFITQLKDGLKNTSHETAANHKVAKWATVTFHLPHHVLKSIASAIVNELKKINQNVAALPVASSVMDRLSYLLPSARPEL
GVGPGRSVDRSLMYSEANRRETFTSWPHVGYRWAQPDPMAQAGFYHQPASSGDDRAMCFTCSVCLVCWEPTDEPWSEHERHSPNCPFVKG
EHTQNVPLSVTLATSPAQFPCTDGTDRISCFGSGSCPHFLAAATKRGKICIWDVSKLMKVHLKFEINAYDPAIVQQLILSGDPSSGVDSR
RPTLAWLEDSSSCSDIPKLEGDSDDLLEDSDSEEHSRSDSVTGHTSQKEAMEVSLDITALSILQQPEKLQWEIVANVLEDTVKDLEELGA
NPCLTNSKSEKTKEKHQEQHNIPFPCLLAGGLLTYKSPATSPISSNSHRSLDGLSRTQGESISEQGSTDNESCTNSELNSPLVRRTLPVL
LLYSIKESDEKAGKIFSQMNNIMSKSLHDDGFTVPQIIEMELDSQEQLLLQDPPVTYIQQFADAAANLTSPDSEKWNSVFPKPGTLVQCL
RLPKFAEEENLCIDSITPCADGIHLLVGLRTCPVESLSAINQVEALNNLNKLNSALCNRRKGELESNLAVVNGANISVIQHESPADVQTP
LIIQPEQRNVSGGYLVLYKMNYATRIVTLEEEPIKIQHIKDPQDTITSLILLPPDILDNREDDCEEPIEDMQLTSKNGFEREKTSDISTL
GHLVITTQGGYVKILDLSNFEILAKVEPPKKEGTEEQDTFVSVIYCSGTDRLCACTKGGELHFLQIGGTCDDIDEADILVDGSLSKGIEP
SSEGSKPLSNPSSPGISGVDLLVDQPFTLEILTSLVELTRFETLTPRFSATVPPCWVEVQQEQQQRRHPQHLHQQHHGDAAQHTRTWKLQ
TDSNSWDEHVFELVLPKACMVGHVDFKFVLNSNITNIPQIQVTLLKNKAPGLGKVNALNIEVEQNGKPSLVDLNEEMQHMDVEESQCLRL
CPFLEDHKEDILCGPVWLASGLDLSGHAGMLTLTSPKLVKGMAGGKYRSFLIHVKAVNERGTEEICNGGMRPVVRLPSLKHQSNKGYSLA
SLLAKVAAGKEKSSNVKNENTSGTRKSENLRGCDLLQEVSVTIRRFKKTSISKERVQRCAMLQFSEFHEKLVNTLCRKTDDGQITEHAQS
LVLDTLCWLAGVHSNGPGSSKEGNENLLSKTRKFLSDIVRVCFFEAGRSIAHKCARFLALCISNGKCDPCQPAFGPVLLKALLDNMSFLP
AATTGGSVYWYFVLLNYVKDEDLAGCSTACASLLTAVSRQLQDRLTPMEALLQTRYGLYSSPFDPVLFDLEMSGSSCKNVYNSSIGVQSD
EIDLSDVLSGNGKVSSCTAAEGSFTSLTGLLEVEPLHFTCVSTSDGTRIERDDAMSSFGVTPAVGGLSSGTVGEASTALSSAAQVALQSL
SHAMASAEQQLQVLQEKQQQLLKLQQQKAKLEAKLHQTTAAAAAAASAVGPVHNSVPSNPVAAPGFFIHPSDVIPPTPKTTPLFMTPPLT
PPNEAVSVVINAELAQLFPGSVIDPPAVNLAAHNKNSNKSRMNPLGSGLALAISHASHFLQPPPHQSIIIERMHSGARRFVTLDFGRPIL
LTDVLIPTCGDLASLSIDIWTLGEEVDGRRLVVATDISTHSLILHDLIPPPVCRFMKITVIGRYGSTNARAKIPLGFYYGHTYILPWESE
LKLMHDPLKGEGESANQPEIDQHLAMMVALQEDIQCRYNLACHRLETLLQSIDLPPLNSANNAQYFLRKPDKAVEEDSRVFSAYQDCIQL
QLQLNLAHNAVQRLKVALGASRKMLSETSNPEDLIQTSSTEQLRTIIRYLLDTLLSLLHASNGHSVPAVLQSTFHAQACEELFKHLCISG
TPKIRLHTGLLLVQLCGGERWWGQFLSNVLQELYNSEQLLIFPQDRVFMLLSCIGQRSLSNSGVLESLLNLLDNLLSPLQPQLPMHRRTE
GVLDIPMISWVVMLVSRLLDYVATVEDEAAAAKKPLNGNQWSFINNNLHTQSLNRSSKGSSSLDRLYSRKIRKQLVHHKQQLNLLKAKQK
ALVEQMEKEKIQSNKGSSYKLLVEQAKLKQATSKHFKDLIRLRRTAEWSRSNLDTEVTTAKESPEIEPLPFTLAHERCISVVQKLVLFLL
SMDFTCHADLLLFVCKVLARIANATRPTIHLCEIVNEPQLERLLLLLVGTDFNRGDISWGGAWAQYSLTCMLQDILAGELLAPVAAEAME
EGTVGDDVGATAGDSDDSLQQSSVQLLETIDEPLTHDITGAPPLSSLEKDKEIDLELLQDLMEVDIDPLDIDLEKDPLAAKVFKPISSTW
YDYWGADYGTYNYNPYIGGLGIPVAKPPANTEKNGSQTVSVSVSQALDARLEVGLEQQAELMLKMMSTLEADSILQALTNTSPTLSQSPT
GTDDSLLGGLQAANQTSQLIIQLSSVPMLNVCFNKLFSMLQVHHVQLESLLQLWLTLSLNSSSTGNKENGADIFLYNANRIPVISLNQAS
ITSFLTVLAWYPNTLLRTWCLVLHSLTLMTNMQLNSGSSSAIGTQESTAHLLVSDPNLIHVLVKFLSGTSPHGTNQHSPQVGPTATQAMQ
EFLTRLQVHLSSTCPQIFSEFLLKLIHILSTERGAFQTGQGPLDAQVKLLEFTLEQNFEVVSVSTISAVIESVTFLVHHYITCSDKVMSR
SGSDSSVGARACFGGLFANLIRPGDAKAVCGEMTRDQLMFDLLKLVNILVQLPLSGNREYSARVSVTTNTTDSVSDEEKVSGGKDGNGSS
TSVQGSPAYVADLVLANQQIMSQILSALGLCNSSAMAMIIGASGLHLTKHENFHGGLDAISVGDGLFTILTTLSKKASTVHMMLQPILTY
MACGYMGRQGSLATCQLSEPLLWFILRVLDTSDALKAFHDMGGVQLICNNMVTSTRAIVNTARSMVSTIMKFLDSGPNKAVDSTLKTRIL
ASEPDNAEGIHNFAPLGTITSSSPTAQPAEVLLQATPPHRRARSAAWSYIFLPEEAWCDLTIHLPAAVLLKEIHIQPHLASLATCPSSVS
VEVSADGVNMLPLSTPVVTSGLTYIKIQLVKAEVASAVCLRLHRPRDASTLGLSQIKLLGLTAFGTTSSATVNNPFLPSEDQVSKTSIGW
LRLLHHCLTHISDLEGMMASAAAPTANLLQTCAALLMSPYCGMHSPNIEVVLVKIGLQSTRIGLKLIDILLRNCAASGSDPTDLNSPLLF
GRLNGLSSDSTIDILYQLGTTQDPGTKDRIQALLKWVSDSARVAAMKRSGRMNYMCPNSSTVEYGLLMPSPSHLHCVAAILWHSYELLVE
YDLPALLDQELFELLFNWSMSLPCNMVLKKAVDSLLCSMCHVHPNYFSLLMGWMGITPPPVQCHHRLSMTDDSKKQDLSSSLTDDSKNAQ
APLALTESHLATLASSSQSPEAIKQLLDSGLPSLLVRSLASFCFSHISSSESIAQSIDISQDKLRRHHVPQQCNKMPITADLVAPILRFL
TEVGNSHIMKDWLGGSEVNPLWTALLFLLCHSGSTSGSHNLGAQQTSARSASLSSAATTGLTTQQRTAIENATVAFFLQCISCHPNNQKL
MAQVLCELFQTSPQRGNLPTSGNISGFIRRLFLQLMLEDEKVTMFLQSPCPLYKGRINATSHVIQHPMYGAGHKFRTLHLPVSTTLSDVL
DRVSDTPSITAKLISEQKDDKEKKNHEEKEKVKAENGFQDNYSVVVASGLKSQSKRAVSATPPRPPSRRGRTIPDKIGSTSGAEAANKII
TVPVFHLFHKLLAGQPLPAEMTLAQLLTLLYDRKLPQGYRSIDLTVKLGSRVITDPSLSKTDSYKRLHPEKDHGDLLASCPEDEALTPGD
ECMDGILDESLLETCPIQSPLQVFAGMGGLALIAERLPMLYPEVIQQVSAPVVTSTTQEKPKDSDQFEWVTIEQSGELVYEAPETVAAEP
PPIKSAVQTMSPIPAHSLAAFGLFLRLPGYAEVLLKERKHAQCLLRLVLGVTDDGEGSHILQSPSANVLPTLPFHVLRSLFSTTPLTTDD
GVLLRRMALEIGALHLILVCLSALSHHSPRVPNSSVNQTEPQVSSSHNPTSTEEQQLYWAKGTGFGTGSTASGWDVEQALTKQRLEEEHV
TCLLQVLASYINPVSSAVNGEAQSSHETRGQNSNALPSVLLELLSQSCLIPAMSSYLRNDSELKPEQVKNMSASEMRNIRLSDFTESLKK

--------------------------------------------------------------

Top

Fusion Gene PPI Analysis for BIRC6-SPAST


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs for BIRC6-SPAST


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

Top

Related Diseases for BIRC6-SPAST


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneBIRC6C0006826Malignant Neoplasms1CTD_human
HgeneBIRC6C0027651Neoplasms1CTD_human
HgeneBIRC6C0086692Benign Neoplasm1CTD_human
TgeneC1866855Spastic paraplegia 4, autosomal dominant46CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneC0026838Muscle Spasticity1GENOMICS_ENGLAND
TgeneC0598589Inherited neuropathies1GENOMICS_ENGLAND
TgeneC1535926Neurodevelopmental Disorders1CTD_human
TgeneC4553743Spasticity, CTCAE1GENOMICS_ENGLAND