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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:CYP3A43-ZSCAN25 (FusionGDB2 ID:HG64816TG221785)

Fusion Gene Summary for CYP3A43-ZSCAN25

check button Fusion gene summary
Fusion gene informationFusion gene name: CYP3A43-ZSCAN25
Fusion gene ID: hg64816tg221785
HgeneTgene
Gene symbol

CYP3A43

ZSCAN25

Gene ID

64816

221785

Gene namecytochrome P450 family 3 subfamily A member 43zinc finger and SCAN domain containing 25
Synonyms-ZNF498
Cytomap('CYP3A43')('ZSCAN25')

7q22.1

7q22.1

Type of geneprotein-codingprotein-coding
Descriptioncytochrome P450 3A43cytochrome P450, family 3, subfamily A, polypeptide 43cytochrome P450, subfamily IIIA, polypeptide 43zinc finger and SCAN domain-containing protein 25zinc finger protein 498
Modification date2020031320200313
UniProtAcc

Q9HB55

.
Ensembl transtripts involved in fusion geneENST00000477658, ENST00000222382, 
ENST00000312017, ENST00000354829, 
ENST00000415413, ENST00000417625, 
ENST00000421837, ENST00000444905, 
ENST00000342499, 
Fusion gene scores* DoF score1 X 1 X 1=13 X 3 X 3=27
# samples 13
** MAII scorelog2(1/1*10)=3.32192809488736log2(3/27*10)=0.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: CYP3A43 [Title/Abstract] AND ZSCAN25 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointCYP3A43(99425809)-ZSCAN25(99226993), # samples:2
Anticipated loss of major functional domain due to fusion event.CYP3A43-ZSCAN25 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
CYP3A43-ZSCAN25 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
CYP3A43-ZSCAN25 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
CYP3A43-ZSCAN25 seems lost the major protein functional domain in Tgene partner, which is a transcription factor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LIHCTCGA-XR-A8TG-01ACYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+


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Fusion Gene ORF analysis for CYP3A43-ZSCAN25

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000477658ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
3UTR-3CDSENST00000477658ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
3UTR-3CDSENST00000477658ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
3UTR-intronENST00000477658ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5CDS-intronENST00000222382ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5CDS-intronENST00000312017ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5CDS-intronENST00000354829ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5CDS-intronENST00000415413ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5CDS-intronENST00000417625ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5CDS-intronENST00000421837ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5CDS-intronENST00000444905ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5UTR-3CDSENST00000342499ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5UTR-3CDSENST00000342499ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5UTR-3CDSENST00000342499ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
5UTR-intronENST00000342499ENST00000466948CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000222382ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000222382ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000222382ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000312017ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000312017ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000312017ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000354829ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000354829ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000354829ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000415413ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000415413ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000415413ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000417625ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000417625ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000417625ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000421837ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000421837ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000421837ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000444905ENST00000262941CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000444905ENST00000334715CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+
Frame-shiftENST00000444905ENST00000394152CYP3A43chr7

99425809

+ZSCAN25chr7

99226993

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for CYP3A43-ZSCAN25


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


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Fusion Protein Features for CYP3A43-ZSCAN25


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:99425809/:99226993)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
CYP3A43

Q9HB55

.
FUNCTION: Exhibits low testosterone 6-beta-hydroxylase activity.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for CYP3A43-ZSCAN25


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for CYP3A43-ZSCAN25


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for CYP3A43-ZSCAN25


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for CYP3A43-ZSCAN25


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneCYP3A43C0033578Prostatic Neoplasms1CTD_human
HgeneCYP3A43C0376358Malignant neoplasm of prostate1CTD_human