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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:CALD1-THSD4 (FusionGDB2 ID:HG800TG79875)

Fusion Gene Summary for CALD1-THSD4

check button Fusion gene summary
Fusion gene informationFusion gene name: CALD1-THSD4
Fusion gene ID: hg800tg79875
HgeneTgene
Gene symbol

CALD1

THSD4

Gene ID

800

79875

Gene namecaldesmon 1thrombospondin type 1 domain containing 4
SynonymsCDM|H-CAD|HCAD|L-CAD|LCAD|NAG22ADAMTSL-6|ADAMTSL6|FVSY9334|PRO34005
Cytomap('CALD1')('THSD4')

7q33

15q23

Type of geneprotein-codingprotein-coding
Descriptioncaldesmontestis secretory sperm-binding protein Li 227nthrombospondin type-1 domain-containing protein 4A disintegrin and metalloproteinase with thrombospondin motifs-like protein 6ADAMTS-like protein 6thrombospondin, type I, domain containing 4
Modification date2020031320200313
UniProtAcc

Q05682

.
Ensembl transtripts involved in fusion geneENST00000361388, ENST00000393118, 
ENST00000361901, ENST00000361675, 
ENST00000417172, ENST00000422748, 
ENST00000424922, ENST00000466704, 
ENST00000495522, ENST00000543443, 
Fusion gene scores* DoF score20 X 22 X 11=484030 X 19 X 13=7410
# samples 2931
** MAII scorelog2(29/4840*10)=-4.06088224214702
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(31/7410*10)=-4.57913342191896
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: CALD1 [Title/Abstract] AND THSD4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointCALD1(134655392)-THSD4(72072881), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Fusion gene information
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4SARCTCGA-DX-A48L-01ACALD1chr7

134655392

-THSD4chr15

72072881

+


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Fusion Gene ORF analysis for CALD1-THSD4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3UTRENST00000361388ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
3UTR-3UTRENST00000361388ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
3UTR-3UTRENST00000393118ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
3UTR-3UTRENST00000393118ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
3UTR-intronENST00000361388ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
3UTR-intronENST00000361388ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
3UTR-intronENST00000393118ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
3UTR-intronENST00000393118ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
5CDS-3UTRENST00000361901ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
5CDS-3UTRENST00000361901ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
5CDS-intronENST00000361901ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
5CDS-intronENST00000361901ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000361675ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000361675ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000417172ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000417172ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000422748ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000422748ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000424922ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000424922ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000466704ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000466704ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000495522ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000495522ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000543443ENST00000261862CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-3UTRENST00000543443ENST00000355327CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000361675ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000361675ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000417172ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000417172ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000422748ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000422748ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000424922ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000424922ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000466704ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000466704ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000495522ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000495522ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000543443ENST00000357769CALD1chr7

134655392

-THSD4chr15

72072881

+
intron-intronENST00000543443ENST00000567838CALD1chr7

134655392

-THSD4chr15

72072881

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for CALD1-THSD4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)


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Fusion Protein Features for CALD1-THSD4


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:134655392/:72072881)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
CALD1

Q05682

.
FUNCTION: Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity). {ECO:0000250, ECO:0000269|PubMed:8227296}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for CALD1-THSD4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for CALD1-THSD4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for CALD1-THSD4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for CALD1-THSD4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneCALD1C0003873Rheumatoid Arthritis1CTD_human
HgeneCALD1C0087031Juvenile-Onset Still Disease1CTD_human
HgeneCALD1C3495559Juvenile arthritis1CTD_human
HgeneCALD1C3714758Juvenile psoriatic arthritis1CTD_human
HgeneCALD1C4552091Polyarthritis, Juvenile, Rheumatoid Factor Negative1CTD_human
HgeneCALD1C4704862Polyarthritis, Juvenile, Rheumatoid Factor Positive1CTD_human
TgeneC0013146Drug abuse1CTD_human
TgeneC0013170Drug habituation1CTD_human
TgeneC0013222Drug Use Disorders1CTD_human
TgeneC0029231Organic Mental Disorders, Substance-Induced1CTD_human
TgeneC0038580Substance Dependence1CTD_human
TgeneC0038586Substance Use Disorders1CTD_human
TgeneC0236969Substance-Related Disorders1CTD_human
TgeneC0740858Substance abuse problem1CTD_human
TgeneC1510472Drug Dependence1CTD_human
TgeneC4316881Prescription Drug Abuse1CTD_human