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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:DTNB-ASXL2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: DTNB-ASXL2
	FusionPDB ID: 24368
	FusionGDB2.0 ID: 24368
		Hgene	Tgene
	Gene symbol	DTNB	ASXL2
	Gene ID	1838	55252
	Gene name	dystrobrevin beta	ASXL transcriptional regulator 2
	Synonyms	-	ASXH2\|SHAPNS
	Cytomap	2p23.3	2p23.3
	Type of gene	protein-coding	protein-coding
	Description	dystrobrevin betaDTN-Bbeta-dystrobrevin	putative Polycomb group protein ASXL2additional sex combs like 2, transcriptional regulatoradditional sex combs-like protein 2polycomb group protein ASXH2
	Modification date	20200327	20200313
	UniProtAcc	Q96EV8	Q76L83
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000288642, ENST00000404103, ENST00000405222, ENST00000406818, ENST00000407038, ENST00000407186, ENST00000407661, ENST00000496972, ENST00000545439, ENST00000472690,	ENST00000272341, ENST00000404843, ENST00000497092, ENST00000336112, ENST00000435504,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	10 X 10 X 7=700	11 X 14 X 8=1232
	# samples	12	16
	** MAII score	log2(12/700*10)=-2.54432051622381 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(16/1232*10)=-2.94485844580754 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: DTNB [Title/Abstract] AND ASXL2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	DTNB(25818955)-ASXL2(25991737), # samples:3 ASXL2(26022254)-DTNB(25819109), # samples:1
Anticipated loss of major functional domain due to fusion event.	ASXL2-DTNB seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ASXL2-DTNB seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. DTNB-ASXL2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DTNB-ASXL2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DTNB-ASXL2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. DTNB-ASXL2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. DTNB-ASXL2 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. DTNB-ASXL2 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. DTNB-ASXL2 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. DTNB-ASXL2 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. ASXL2-DTNB seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. ASXL2-DTNB seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF. ASXL2-DTNB seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. ASXL2-DTNB seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	ASXL2	GO:0035360	positive regulation of peroxisome proliferator activated receptor signaling pathway	21047783
Tgene	ASXL2	GO:0045600	positive regulation of fat cell differentiation	21047783
Tgene	ASXL2	GO:0045944	positive regulation of transcription by RNA polymerase II	21047783

Fusion gene breakpoints across DTNB (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ASXL2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BRCA	TCGA-BH-A18V-06A	DTNB	chr2	25818955	-	ASXL2	chr2	25973282	-
ChimerDB4	ESCA	TCGA-L5-A8NE-01A	DTNB	chr2	25818955	-	ASXL2	chr2	25991737	-
ChimerDB4	ESCA	TCGA-L5-A8NE	DTNB	chr2	25818955	-	ASXL2	chr2	25991737	-
ChimerDB4	STAD	TCGA-BR-4357-01A	DTNB	chr2	25754342	-	ASXL2	chr2	25994409	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000496972	DTNB	chr2	25818955	-	ENST00000435504	ASXL2	chr2	25991737	-	12819	739	307	4542	1411
ENST00000496972	DTNB	chr2	25818955	-	ENST00000336112	ASXL2	chr2	25991737	-	8880	739	307	4542	1411

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000496972	ENST00000435504	DTNB	chr2	25818955	-	ASXL2	chr2	25991737	-	0.000196825	0.99980325
ENST00000496972	ENST00000336112	DTNB	chr2	25818955	-	ASXL2	chr2	25991737	-	0.00033044	0.99966955

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>24368_24368_1_DTNB-ASXL2_DTNB_chr2_25818955_ENST00000496972_ASXL2_chr2_25991737_ENST00000336112_length(amino acids)=1411AA_BP=144
MIEAFRDNGLNTLDHTTEISVSRLETVISSIYYQLNKRLPSTHQISVEQSISLLLNFMIAAYDSEGRGKLTVFSVKAMLATMCGGKMLDK
LRYVFSQMSDSNGLMIFSKFDQFLKEVLKLPTAVFEGPSFGYTEHSVRTCFPQQALKQQQQKKQQQQCRPSISISSNQHLSLKTVKAASD
SVPAKPATWEGKQSDGQTGSPQNSNSSFSSSVKVENTLLGLGKKSFQRSERLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSV
LPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVEPWKEQFFESYYGQSSGL
SLEDSKKLTASPSDPKVKKTPAEQPKSMPVSEASLIRIVPVVSQSECKEEALQMSSPGRKEECESQGEVQPNFSTSSEPLLSSALNTHEL
SSILPIKCPKDEDLLEQKPVTSAEQESEKNHLTTASNYNKSESQESLVTSPSKPKSPGVEKPIVKPTAGAGPQETNMKEPLATLVDQSPE
SLKRKSSLTQEEAPVSWEKRPRVTENRQHQQPFQVSPQPFLNRGDRIQVRKVPPLKIPVSRISPMPFHPSQVSPRARFPVSITSPNRTGA
RTLADIKAKAQLVKAQRAAAAAAAAAAAAASVGGTIPGPGPGGGQGPGEGGEGQTARGGSPGSDRVSETGKGPTLELAGTGSRGGTRELL
PCGPETQPQSETKTTPSQAQPHSVSGAQLQQTPPVPPTPAVSGACTSVPSPAHIEKLDNEKLNPTRATATVASVSHPQGPSSCRQEKAPS
PTGPALISGASPVHCAADGTVELKAGPSKNIPNPSASSKTDASVPVAVTPSPLTSLLTTATLEKLPVPQVSATTAPAGSAPPSSTLPAAS
SLKTPGTSLNMNGPTLRPTSSIPANNPLVTQLLQGKDVPMEQILPKPLTKVEMKTVPLTAKEERGMGALIATNTTENSTREEVNERQSHP
ATQQQLGKTLQSKQLPQVPRPLQLFSAKELRDSSIDTHQYHEGLSKATQDQILQTLIQRVRRQNLLSVVPPSQFNFAHSGFQLEDISTSQ
RFMLGFAGRRTSKPAMAGHYLLNISTYGRGSESFRRTHSVNPEDRFCLSSPTEALKMGYTDCKNATGESSSSKEDDTDEESTGDEQESVT
VKEEPQVSQSAGKGDTSSGPHSRETLSTSDCLASKNVKAEIPLNEQTTLSKENYLFTRGQTFDEKTLARDLIQAAQKQMAHAVRGKAIRS
SPELFSSTVLPLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAVPGSQVSSNVGDVMSFSVTVTTIPASQAMN

--------------------------------------------------------------

>24368_24368_2_DTNB-ASXL2_DTNB_chr2_25818955_ENST00000496972_ASXL2_chr2_25991737_ENST00000435504_length(amino acids)=1411AA_BP=144
MIEAFRDNGLNTLDHTTEISVSRLETVISSIYYQLNKRLPSTHQISVEQSISLLLNFMIAAYDSEGRGKLTVFSVKAMLATMCGGKMLDK
LRYVFSQMSDSNGLMIFSKFDQFLKEVLKLPTAVFEGPSFGYTEHSVRTCFPQQALKQQQQKKQQQQCRPSISISSNQHLSLKTVKAASD
SVPAKPATWEGKQSDGQTGSPQNSNSSFSSSVKVENTLLGLGKKSFQRSERLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSV
LPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVEPWKEQFFESYYGQSSGL
SLEDSKKLTASPSDPKVKKTPAEQPKSMPVSEASLIRIVPVVSQSECKEEALQMSSPGRKEECESQGEVQPNFSTSSEPLLSSALNTHEL
SSILPIKCPKDEDLLEQKPVTSAEQESEKNHLTTASNYNKSESQESLVTSPSKPKSPGVEKPIVKPTAGAGPQETNMKEPLATLVDQSPE
SLKRKSSLTQEEAPVSWEKRPRVTENRQHQQPFQVSPQPFLNRGDRIQVRKVPPLKIPVSRISPMPFHPSQVSPRARFPVSITSPNRTGA
RTLADIKAKAQLVKAQRAAAAAAAAAAAAASVGGTIPGPGPGGGQGPGEGGEGQTARGGSPGSDRVSETGKGPTLELAGTGSRGGTRELL
PCGPETQPQSETKTTPSQAQPHSVSGAQLQQTPPVPPTPAVSGACTSVPSPAHIEKLDNEKLNPTRATATVASVSHPQGPSSCRQEKAPS
PTGPALISGASPVHCAADGTVELKAGPSKNIPNPSASSKTDASVPVAVTPSPLTSLLTTATLEKLPVPQVSATTAPAGSAPPSSTLPAAS
SLKTPGTSLNMNGPTLRPTSSIPANNPLVTQLLQGKDVPMEQILPKPLTKVEMKTVPLTAKEERGMGALIATNTTENSTREEVNERQSHP
ATQQQLGKTLQSKQLPQVPRPLQLFSAKELRDSSIDTHQYHEGLSKATQDQILQTLIQRVRRQNLLSVVPPSQFNFAHSGFQLEDISTSQ
RFMLGFAGRRTSKPAMAGHYLLNISTYGRGSESFRRTHSVNPEDRFCLSSPTEALKMGYTDCKNATGESSSSKEDDTDEESTGDEQESVT
VKEEPQVSQSAGKGDTSSGPHSRETLSTSDCLASKNVKAEIPLNEQTTLSKENYLFTRGQTFDEKTLARDLIQAAQKQMAHAVRGKAIRS
SPELFSSTVLPLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAVPGSQVSSNVGDVMSFSVTVTTIPASQAMN

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:25818955/chr2:25991737)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
DTNB Q96EV8	ASXL2 Q76L83
FUNCTION: Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway. {ECO:0000269\|PubMed:15345706, ECO:0000269\|PubMed:16837549, ECO:0000269\|PubMed:17182842, ECO:0000269\|PubMed:17989303, ECO:0000269\|PubMed:19094965, ECO:0000269\|PubMed:20180862, ECO:0000269\|PubMed:20921223}.	FUNCTION: Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via methylation of histones, rendering chromatin heritably changed in its expressibility (By similarity). Involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as peroxisome proliferator-activated receptor gamma (PPARG). Acts as coactivator for PPARG and enhances its adipocyte differentiation-inducing activity; the function seems to involve differential recruitment of acetylated and methylated histone H3. {ECO:0000250, ECO:0000269\|PubMed:21047783}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

1173_1176

919.0

Compositional bias

Note=Poly-Ser

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

303_307

919.0

Compositional bias

Note=Poly-Leu

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

654_684

919.0

Compositional bias

Note=Ala-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

687_739

919.0

Compositional bias

Note=Gly-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

95_235

919.0

Compositional bias

Note=Ser-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

1173_1176

919.0

Compositional bias

Note=Poly-Ser

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

303_307

919.0

Compositional bias

Note=Poly-Leu

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

654_684

919.0

Compositional bias

Note=Ala-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

687_739

919.0

Compositional bias

Note=Gly-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

95_235

919.0

Compositional bias

Note=Ser-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

1173_1176

168.0

1436.0

Compositional bias

Note=Poly-Ser

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

303_307

168.0

1436.0

Compositional bias

Note=Poly-Leu

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

654_684

168.0

1436.0

Compositional bias

Note=Ala-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

687_739

168.0

1436.0

Compositional bias

Note=Gly-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

11_86

919.0

Domain

HTH HARE-type

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

274_383

919.0

Domain

DEUBAD

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

11_86

919.0

Domain

HTH HARE-type

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

274_383

919.0

Domain

DEUBAD

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

274_383

168.0

1436.0

Domain

DEUBAD

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

174_178

919.0

Motif

Nuclear localization signal

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

887_891

919.0

Motif

Note=LXXLL motif 2

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

174_178

919.0

Motif

Nuclear localization signal

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

887_891

919.0

Motif

Note=LXXLL motif 2

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

174_178

168.0

1436.0

Motif

Nuclear localization signal

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

887_891

168.0

1436.0

Motif

Note=LXXLL motif 2

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000272341

1397_1434

919.0

Zinc finger

Note=PHD-type%3B atypical

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000404843

1397_1434

919.0

Zinc finger

Note=PHD-type%3B atypical

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

1397_1434

168.0

1436.0

Zinc finger

Note=PHD-type%3B atypical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000404103

428_529

201.0

607.3333333333334

Coiled coil

Ontology_term=ECO:0000255

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000405222

428_529

201.0

570.3333333333334

Coiled coil

Ontology_term=ECO:0000255

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000406818

428_529

201.0

637.3333333333334

Coiled coil

Ontology_term=ECO:0000255

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000407038

428_529

201.0

577.3333333333334

Coiled coil

Ontology_term=ECO:0000255

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000407661

428_529

201.0

619.3333333333334

Coiled coil

Ontology_term=ECO:0000255

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000496972

428_529

144.0

581.3333333333334

Coiled coil

Ontology_term=ECO:0000255

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000404103

399_448

201.0

607.3333333333334

Region

Note=Syntrophin-binding region

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000405222

399_448

201.0

570.3333333333334

Region

Note=Syntrophin-binding region

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000406818

399_448

201.0

637.3333333333334

Region

Note=Syntrophin-binding region

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000407038

399_448

201.0

577.3333333333334

Region

Note=Syntrophin-binding region

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000407661

399_448

201.0

619.3333333333334

Region

Note=Syntrophin-binding region

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000496972

399_448

144.0

581.3333333333334

Region

Note=Syntrophin-binding region

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000404103

237_284

201.0

607.3333333333334

Zinc finger

ZZ-type

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000405222

237_284

201.0

570.3333333333334

Zinc finger

ZZ-type

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000406818

237_284

201.0

637.3333333333334

Zinc finger

ZZ-type

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000407038

237_284

201.0

577.3333333333334

Zinc finger

ZZ-type

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000407661

237_284

201.0

619.3333333333334

Zinc finger

ZZ-type

Hgene

DTNB

chr2:25818955

chr2:25991737

ENST00000496972

237_284

144.0

581.3333333333334

Zinc finger

ZZ-type

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

95_235

168.0

1436.0

Compositional bias

Note=Ser-rich

Tgene

ASXL2

chr2:25818955

chr2:25991737

ENST00000435504

11_86

168.0

1436.0

Domain

HTH HARE-type

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>2046_DTNB_25818955_ASXL2_25991737_ranked_0.pdb	DTNB	25818955	25818955	ENST00000336112	ASXL2	chr2	25991737	-	MIEAFRDNGLNTLDHTTEISVSRLETVISSIYYQLNKRLPSTHQISVEQSISLLLNFMIAAYDSEGRGKLTVFSVKAMLATMCGGKMLDK LRYVFSQMSDSNGLMIFSKFDQFLKEVLKLPTAVFEGPSFGYTEHSVRTCFPQQALKQQQQKKQQQQCRPSISISSNQHLSLKTVKAASD SVPAKPATWEGKQSDGQTGSPQNSNSSFSSSVKVENTLLGLGKKSFQRSERLHTRQMKRTKCADIDVETPDSILVNTNLRALINKHTFSV LPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVEPWKEQFFESYYGQSSGL SLEDSKKLTASPSDPKVKKTPAEQPKSMPVSEASLIRIVPVVSQSECKEEALQMSSPGRKEECESQGEVQPNFSTSSEPLLSSALNTHEL SSILPIKCPKDEDLLEQKPVTSAEQESEKNHLTTASNYNKSESQESLVTSPSKPKSPGVEKPIVKPTAGAGPQETNMKEPLATLVDQSPE SLKRKSSLTQEEAPVSWEKRPRVTENRQHQQPFQVSPQPFLNRGDRIQVRKVPPLKIPVSRISPMPFHPSQVSPRARFPVSITSPNRTGA RTLADIKAKAQLVKAQRAAAAAAAAAAAAASVGGTIPGPGPGGGQGPGEGGEGQTARGGSPGSDRVSETGKGPTLELAGTGSRGGTRELL PCGPETQPQSETKTTPSQAQPHSVSGAQLQQTPPVPPTPAVSGACTSVPSPAHIEKLDNEKLNPTRATATVASVSHPQGPSSCRQEKAPS PTGPALISGASPVHCAADGTVELKAGPSKNIPNPSASSKTDASVPVAVTPSPLTSLLTTATLEKLPVPQVSATTAPAGSAPPSSTLPAAS SLKTPGTSLNMNGPTLRPTSSIPANNPLVTQLLQGKDVPMEQILPKPLTKVEMKTVPLTAKEERGMGALIATNTTENSTREEVNERQSHP ATQQQLGKTLQSKQLPQVPRPLQLFSAKELRDSSIDTHQYHEGLSKATQDQILQTLIQRVRRQNLLSVVPPSQFNFAHSGFQLEDISTSQ RFMLGFAGRRTSKPAMAGHYLLNISTYGRGSESFRRTHSVNPEDRFCLSSPTEALKMGYTDCKNATGESSSSKEDDTDEESTGDEQESVT VKEEPQVSQSAGKGDTSSGPHSRETLSTSDCLASKNVKAEIPLNEQTTLSKENYLFTRGQTFDEKTLARDLIQAAQKQMAHAVRGKAIRS SPELFSSTVLPLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAVPGSQVSSNVGDVMSFSVTVTTIPASQAMN	1411

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

DTNB_pLDDT.png

ASXL2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
DTNB
ASXL2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to DTNB-ASXL2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to DTNB-ASXL2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Tgene	ASXL2	C0005684	Malignant neoplasm of urinary bladder	1	CTD_human
Tgene	ASXL2	C0005695	Bladder Neoplasm	1	CTD_human
Tgene	ASXL2	C0023467	Leukemia, Myelocytic, Acute	1	CTD_human
Tgene	ASXL2	C0026998	Acute Myeloid Leukemia, M1	1	CTD_human
Tgene	ASXL2	C1879321	Acute Myeloid Leukemia (AML-M2)	1	CTD_human
Tgene	ASXL2	C4310672	SHASHI-PENA SYNDROME	1	GENOMICS_ENGLAND