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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:NOL8-FXN

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NOL8-FXN
	FusionPDB ID: 59570
	FusionGDB2.0 ID: 59570
		Hgene	Tgene
	Gene symbol	NOL8	FXN
	Gene ID	55035	2395
	Gene name	nucleolar protein 8	frataxin
	Synonyms	C9orf34\|NOP132\|bA62C3.3\|bA62C3.4	CyaY\|FA\|FARR\|FRDA\|X25
	Cytomap	9q22.31	9q21.11
	Type of gene	protein-coding	protein-coding
	Description	nucleolar protein 8nucleolar protein Nop132	frataxin, mitochondrialFriedreich ataxia protein
	Modification date	20200313	20200315
	UniProtAcc	Q76FK4	Q16595
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000358855, ENST00000442668, ENST00000535387, ENST00000542053, ENST00000545558, ENST00000543985,	ENST00000498653, ENST00000377270, ENST00000396364, ENST00000396366,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	2 X 2 X 2=8	8 X 8 X 7=448
	# samples	2	10
	** MAII score	log2(2/8*10)=1.32192809488736	log2(10/448*10)=-2.16349873228288 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: NOL8 [Title/Abstract] AND FXN [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NOL8(95076549)-FXN(71661301), # samples:3
Anticipated loss of major functional domain due to fusion event.	NOL8-FXN seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NOL8-FXN seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NOL8-FXN seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NOL8-FXN seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NOL8-FXN seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. NOL8-FXN seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. NOL8-FXN seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	FXN	GO:0010722	regulation of ferrochelatase activity	15123683
Tgene	FXN	GO:0016226	iron-sulfur cluster assembly	29491838
Tgene	FXN	GO:0016540	protein autoprocessing	12785837
Tgene	FXN	GO:0018283	iron incorporation into metallo-sulfur cluster	12785837
Tgene	FXN	GO:0051349	positive regulation of lyase activity	20053667
Tgene	FXN	GO:0070301	cellular response to hydrogen peroxide	15641778

Fusion gene breakpoints across NOL8 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across FXN (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	TGCT	TCGA-2G-AAM4-01A	NOL8	chr9	95076549	-	FXN	chr9	71661301	+
ChimerDB4	TGCT	TCGA-2G-AAM4	NOL8	chr9	95076549	-	FXN	chr9	71661301	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000545558	NOL8	chr9	95076549	-	ENST00000396364	FXN	chr9	71661301	+	3446	2851	493	3201	902
ENST00000545558	NOL8	chr9	95076549	-	ENST00000377270	FXN	chr9	71661301	+	4770	2851	493	3318	941
ENST00000545558	NOL8	chr9	95076549	-	ENST00000396366	FXN	chr9	71661301	+	3587	2851	493	3276	927
ENST00000535387	NOL8	chr9	95076549	-	ENST00000396364	FXN	chr9	71661301	+	2953	2358	0	2708	902
ENST00000535387	NOL8	chr9	95076549	-	ENST00000377270	FXN	chr9	71661301	+	4277	2358	0	2825	941
ENST00000535387	NOL8	chr9	95076549	-	ENST00000396366	FXN	chr9	71661301	+	3094	2358	0	2783	927

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000545558	ENST00000396364	NOL8	chr9	95076549	-	FXN	chr9	71661301	+	0.000741245	0.9992587
ENST00000545558	ENST00000377270	NOL8	chr9	95076549	-	FXN	chr9	71661301	+	0.000358934	0.99964106
ENST00000545558	ENST00000396366	NOL8	chr9	95076549	-	FXN	chr9	71661301	+	0.000494948	0.99950504
ENST00000535387	ENST00000396364	NOL8	chr9	95076549	-	FXN	chr9	71661301	+	0.001133047	0.99886703
ENST00000535387	ENST00000377270	NOL8	chr9	95076549	-	FXN	chr9	71661301	+	0.000437277	0.9995627
ENST00000535387	ENST00000396366	NOL8	chr9	95076549	-	FXN	chr9	71661301	+	0.000722149	0.9992779

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>59570_59570_1_NOL8-FXN_NOL8_chr9_95076549_ENST00000535387_FXN_chr9_71661301_ENST00000377270_length(amino acids)=941AA_BP=786
MKVNRETKRLYVGGLSQDISEADLQNQFSRFGEVSDVEIITRKDDQGNPQKVFAYINISVAEADLKKCMSVLNKTKWKGGTLQIQLAKES
FLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQHKRKIIKYDPSKYCHNLKKIGE
DFSNTIPISSLTWELEGGNDPMSKKRRGEFSDFHGPPKKIIKVQKDESSTGSLAMSTRPRRVIERPPLTQQQAAQKRTCDSITPSKSSPV
PVSDTQKLKNLPFKTSGLETAKKRNSISDDDTDSEDELRMMIAKEENLQRTTQPSINESESDPFEVVRDDFKSGVHKLHSLIGLGIKNRV
SCHDSDDDIMRNDREYDSGDTDEIIAMKKNVAKVKNSTEFSQMEKSTKKTSFKNRENCELSDHCIKLQKRKSNVESALSHGLKSLNRKSP
SHSSSSEDADSASELADSEGGEEYNAMMKNCLRVNLTLADLEQLAGSDLKVPNEDTKSDGPETTTQCKFDRGSKSPKTPTGLRRGRQCIR
PAEIVASLLEGEENTCGKQKPKENNLKPKFQAFKGVGCLYEKESMKKSLKDSVASNNKDQNSMKHEDPSIISMEDGSPYVNGSLGEVTPC
QHAKKANGPNYIQPQKRQTTFESQDRKAVSPSSSEKRSKNPISRPLEGKKSLSLSAKTHNIGFDKDSCHSTTKTEASQEERSDSSGLTSL
KKSPKVSSKDTREIKTDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLSSNQRGLNQIWNVKKQSVYLMNLR
KSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWT

--------------------------------------------------------------

>59570_59570_2_NOL8-FXN_NOL8_chr9_95076549_ENST00000535387_FXN_chr9_71661301_ENST00000396364_length(amino acids)=902AA_BP=786
MKVNRETKRLYVGGLSQDISEADLQNQFSRFGEVSDVEIITRKDDQGNPQKVFAYINISVAEADLKKCMSVLNKTKWKGGTLQIQLAKES
FLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQHKRKIIKYDPSKYCHNLKKIGE
DFSNTIPISSLTWELEGGNDPMSKKRRGEFSDFHGPPKKIIKVQKDESSTGSLAMSTRPRRVIERPPLTQQQAAQKRTCDSITPSKSSPV
PVSDTQKLKNLPFKTSGLETAKKRNSISDDDTDSEDELRMMIAKEENLQRTTQPSINESESDPFEVVRDDFKSGVHKLHSLIGLGIKNRV
SCHDSDDDIMRNDREYDSGDTDEIIAMKKNVAKVKNSTEFSQMEKSTKKTSFKNRENCELSDHCIKLQKRKSNVESALSHGLKSLNRKSP
SHSSSSEDADSASELADSEGGEEYNAMMKNCLRVNLTLADLEQLAGSDLKVPNEDTKSDGPETTTQCKFDRGSKSPKTPTGLRRGRQCIR
PAEIVASLLEGEENTCGKQKPKENNLKPKFQAFKGVGCLYEKESMKKSLKDSVASNNKDQNSMKHEDPSIISMEDGSPYVNGSLGEVTPC
QHAKKANGPNYIQPQKRQTTFESQDRKAVSPSSSEKRSKNPISRPLEGKKSLSLSAKTHNIGFDKDSCHSTTKTEASQEERSDSSGLTSL
KKSPKVSSKDTREIKTDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLSSNQRGLNQIWNVKKQSVYLMNLR
KSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSRLTWLLWLF

--------------------------------------------------------------

>59570_59570_3_NOL8-FXN_NOL8_chr9_95076549_ENST00000535387_FXN_chr9_71661301_ENST00000396366_length(amino acids)=927AA_BP=786
MKVNRETKRLYVGGLSQDISEADLQNQFSRFGEVSDVEIITRKDDQGNPQKVFAYINISVAEADLKKCMSVLNKTKWKGGTLQIQLAKES
FLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQHKRKIIKYDPSKYCHNLKKIGE
DFSNTIPISSLTWELEGGNDPMSKKRRGEFSDFHGPPKKIIKVQKDESSTGSLAMSTRPRRVIERPPLTQQQAAQKRTCDSITPSKSSPV
PVSDTQKLKNLPFKTSGLETAKKRNSISDDDTDSEDELRMMIAKEENLQRTTQPSINESESDPFEVVRDDFKSGVHKLHSLIGLGIKNRV
SCHDSDDDIMRNDREYDSGDTDEIIAMKKNVAKVKNSTEFSQMEKSTKKTSFKNRENCELSDHCIKLQKRKSNVESALSHGLKSLNRKSP
SHSSSSEDADSASELADSEGGEEYNAMMKNCLRVNLTLADLEQLAGSDLKVPNEDTKSDGPETTTQCKFDRGSKSPKTPTGLRRGRQCIR
PAEIVASLLEGEENTCGKQKPKENNLKPKFQAFKGVGCLYEKESMKKSLKDSVASNNKDQNSMKHEDPSIISMEDGSPYVNGSLGEVTPC
QHAKKANGPNYIQPQKRQTTFESQDRKAVSPSSSEKRSKNPISRPLEGKKSLSLSAKTHNIGFDKDSCHSTTKTEASQEERSDSSGLTSL
KKSPKVSSKDTREIKTDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLSSNQRGLNQIWNVKKQSVYLMNLR
KSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSRYVVDLSVM

--------------------------------------------------------------

>59570_59570_4_NOL8-FXN_NOL8_chr9_95076549_ENST00000545558_FXN_chr9_71661301_ENST00000377270_length(amino acids)=941AA_BP=786
MKVNRETKRLYVGGLSQDISEADLQNQFSRFGEVSDVEIITRKDDQGNPQKVFAYINISVAEADLKKCMSVLNKTKWKGGTLQIQLAKES
FLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQHKRKIIKYDPSKYCHNLKKIGE
DFSNTIPISSLTWELEGGNDPMSKKRRGEFSDFHGPPKKIIKVQKDESSTGSLAMSTRPRRVIERPPLTQQQAAQKRTCDSITPSKSSPV
PVSDTQKLKNLPFKTSGLETAKKRNSISDDDTDSEDELRMMIAKEENLQRTTQPSINESESDPFEVVRDDFKSGVHKLHSLIGLGIKNRV
SCHDSDDDIMRNDREYDSGDTDEIIAMKKNVAKVKNSTEFSQMEKSTKKTSFKNRENCELSDHCIKLQKRKSNVESALSHGLKSLNRKSP
SHSSSSEDADSASELADSEGGEEYNAMMKNCLRVNLTLADLEQLAGSDLKVPNEDTKSDGPETTTQCKFDRGSKSPKTPTGLRRGRQCIR
PAEIVASLLEGEENTCGKQKPKENNLKPKFQAFKGVGCLYEKESMKKSLKDSVASNNKDQNSMKHEDPSIISMEDGSPYVNGSLGEVTPC
QHAKKANGPNYIQPQKRQTTFESQDRKAVSPSSSEKRSKNPISRPLEGKKSLSLSAKTHNIGFDKDSCHSTTKTEASQEERSDSSGLTSL
KKSPKVSSKDTREIKTDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLSSNQRGLNQIWNVKKQSVYLMNLR
KSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWT

--------------------------------------------------------------

>59570_59570_5_NOL8-FXN_NOL8_chr9_95076549_ENST00000545558_FXN_chr9_71661301_ENST00000396364_length(amino acids)=902AA_BP=786
MKVNRETKRLYVGGLSQDISEADLQNQFSRFGEVSDVEIITRKDDQGNPQKVFAYINISVAEADLKKCMSVLNKTKWKGGTLQIQLAKES
FLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQHKRKIIKYDPSKYCHNLKKIGE
DFSNTIPISSLTWELEGGNDPMSKKRRGEFSDFHGPPKKIIKVQKDESSTGSLAMSTRPRRVIERPPLTQQQAAQKRTCDSITPSKSSPV
PVSDTQKLKNLPFKTSGLETAKKRNSISDDDTDSEDELRMMIAKEENLQRTTQPSINESESDPFEVVRDDFKSGVHKLHSLIGLGIKNRV
SCHDSDDDIMRNDREYDSGDTDEIIAMKKNVAKVKNSTEFSQMEKSTKKTSFKNRENCELSDHCIKLQKRKSNVESALSHGLKSLNRKSP
SHSSSSEDADSASELADSEGGEEYNAMMKNCLRVNLTLADLEQLAGSDLKVPNEDTKSDGPETTTQCKFDRGSKSPKTPTGLRRGRQCIR
PAEIVASLLEGEENTCGKQKPKENNLKPKFQAFKGVGCLYEKESMKKSLKDSVASNNKDQNSMKHEDPSIISMEDGSPYVNGSLGEVTPC
QHAKKANGPNYIQPQKRQTTFESQDRKAVSPSSSEKRSKNPISRPLEGKKSLSLSAKTHNIGFDKDSCHSTTKTEASQEERSDSSGLTSL
KKSPKVSSKDTREIKTDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLSSNQRGLNQIWNVKKQSVYLMNLR
KSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSRLTWLLWLF

--------------------------------------------------------------

>59570_59570_6_NOL8-FXN_NOL8_chr9_95076549_ENST00000545558_FXN_chr9_71661301_ENST00000396366_length(amino acids)=927AA_BP=786
MKVNRETKRLYVGGLSQDISEADLQNQFSRFGEVSDVEIITRKDDQGNPQKVFAYINISVAEADLKKCMSVLNKTKWKGGTLQIQLAKES
FLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQHKRKIIKYDPSKYCHNLKKIGE
DFSNTIPISSLTWELEGGNDPMSKKRRGEFSDFHGPPKKIIKVQKDESSTGSLAMSTRPRRVIERPPLTQQQAAQKRTCDSITPSKSSPV
PVSDTQKLKNLPFKTSGLETAKKRNSISDDDTDSEDELRMMIAKEENLQRTTQPSINESESDPFEVVRDDFKSGVHKLHSLIGLGIKNRV
SCHDSDDDIMRNDREYDSGDTDEIIAMKKNVAKVKNSTEFSQMEKSTKKTSFKNRENCELSDHCIKLQKRKSNVESALSHGLKSLNRKSP
SHSSSSEDADSASELADSEGGEEYNAMMKNCLRVNLTLADLEQLAGSDLKVPNEDTKSDGPETTTQCKFDRGSKSPKTPTGLRRGRQCIR
PAEIVASLLEGEENTCGKQKPKENNLKPKFQAFKGVGCLYEKESMKKSLKDSVASNNKDQNSMKHEDPSIISMEDGSPYVNGSLGEVTPC
QHAKKANGPNYIQPQKRQTTFESQDRKAVSPSSSEKRSKNPISRPLEGKKSLSLSAKTHNIGFDKDSCHSTTKTEASQEERSDSSGLTSL
KKSPKVSSKDTREIKTDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLSSNQRGLNQIWNVKKQSVYLMNLR
KSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSRYVVDLSVM

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr9:95076549/chr9:71661301)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
NOL8 Q76FK4	FXN Q16595
FUNCTION: Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells. {ECO:0000269\|PubMed:14660641, ECO:0000269\|PubMed:15132771, ECO:0000269\|PubMed:16963496}.	FUNCTION: Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1. {ECO:0000269\|PubMed:12785837, ECO:0000269\|PubMed:15247478, ECO:0000269\|PubMed:15641778, ECO:0000269\|PubMed:16239244, ECO:0000269\|PubMed:16608849, ECO:0000269\|PubMed:20053667}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000442668

753_779

786.0

1168.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000535387

753_779

786.0

1130.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000545558

753_779

786.0

1168.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000358855

8_89

718.0

1100.0

Domain

RRM

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000442668

8_89

786.0

1168.0

Domain

RRM

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000535387

8_89

786.0

1130.0

Domain

RRM

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000542053

8_89

718.0

1100.0

Domain

RRM

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000545558

8_89

786.0

1168.0

Domain

RRM

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000358855

753_779

718.0

1100.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000358855

886_924

718.0

1100.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000442668

886_924

786.0

1168.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000535387

886_924

786.0

1130.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000542053

753_779

718.0

1100.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000542053

886_924

718.0

1100.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000545558

886_924

786.0

1168.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000358855

1113_1116

718.0

1100.0

Compositional bias

Poly-Phe

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000442668

1113_1116

786.0

1168.0

Compositional bias

Poly-Phe

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000535387

1113_1116

786.0

1130.0

Compositional bias

Poly-Phe

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000542053

1113_1116

718.0

1100.0

Compositional bias

Poly-Phe

Hgene

NOL8

chr9:95076549

chr9:71661301

ENST00000545558

1113_1116

786.0

1168.0

Compositional bias

Poly-Phe

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1738_NOL8_95076549_FXN_71661301_ranked_0.pdb	NOL8	95076549	95076549	ENST00000396366	FXN	chr9	71661301	+	MKVNRETKRLYVGGLSQDISEADLQNQFSRFGEVSDVEIITRKDDQGNPQKVFAYINISVAEADLKKCMSVLNKTKWKGGTLQIQLAKES FLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQHKRKIIKYDPSKYCHNLKKIGE DFSNTIPISSLTWELEGGNDPMSKKRRGEFSDFHGPPKKIIKVQKDESSTGSLAMSTRPRRVIERPPLTQQQAAQKRTCDSITPSKSSPV PVSDTQKLKNLPFKTSGLETAKKRNSISDDDTDSEDELRMMIAKEENLQRTTQPSINESESDPFEVVRDDFKSGVHKLHSLIGLGIKNRV SCHDSDDDIMRNDREYDSGDTDEIIAMKKNVAKVKNSTEFSQMEKSTKKTSFKNRENCELSDHCIKLQKRKSNVESALSHGLKSLNRKSP SHSSSSEDADSASELADSEGGEEYNAMMKNCLRVNLTLADLEQLAGSDLKVPNEDTKSDGPETTTQCKFDRGSKSPKTPTGLRRGRQCIR PAEIVASLLEGEENTCGKQKPKENNLKPKFQAFKGVGCLYEKESMKKSLKDSVASNNKDQNSMKHEDPSIISMEDGSPYVNGSLGEVTPC QHAKKANGPNYIQPQKRQTTFESQDRKAVSPSSSEKRSKNPISRPLEGKKSLSLSAKTHNIGFDKDSCHSTTKTEASQEERSDSSGLTSL KKSPKVSSKDTREIKTDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLSSNQRGLNQIWNVKKQSVYLMNLR KSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWT	941

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

NOL8_pLDDT.png

FXN_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
NOL8
FXN

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to NOL8-FXN

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to NOL8-FXN

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source