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Center for Computational Systems Medicine level1
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:DDX5-PRKCB

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: DDX5-PRKCB
FusionPDB ID: 22091
FusionGDB2.0 ID: 22091
HgeneTgene
Gene symbol

DDX5

PRKCB

Gene ID

1655

5579

Gene nameDEAD-box helicase 5protein kinase C beta
SynonymsG17P1|HLR1|HUMP68|p68PKC-beta|PKCB|PKCI(2)|PKCbeta|PRKCB1|PRKCB2
Cytomap

17q23.3

16p12.2-p12.1

Type of geneprotein-codingprotein-coding
Descriptionprobable ATP-dependent RNA helicase DDX5ATP-dependent RNA helicase DDX5DEAD (Asp-Glu-Ala-Asp) box helicase 5DEAD (Asp-Glu-Ala-Asp) box polypeptide 5DEAD box protein 5DEAD box-5DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)RNA hprotein kinase C beta typePKC-Bprotein kinase C, beta 1 polypeptide
Modification date2020031320200313
UniProtAcc

Q5T1V6

.
Ensembl transtripts involved in fusion geneENST idsENST00000225792, ENST00000450599, 
ENST00000578804, ENST00000580026, 
ENST00000482000, ENST00000498058, 
ENST00000303531, ENST00000321728, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score33 X 25 X 10=825020 X 15 X 13=3900
# samples 2725
** MAII scorelog2(27/8250*10)=-4.93336280696971
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(25/3900*10)=-3.96347412397489
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: DDX5 [Title/Abstract] AND PRKCB [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)DDX5(62502192)-PRKCB(24166004), # samples:1
Anticipated loss of major functional domain due to fusion event.DDX5-PRKCB seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DDX5-PRKCB seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DDX5-PRKCB seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
DDX5-PRKCB seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneDDX5

GO:0000122

negative regulation of transcription by RNA polymerase II

15298701

HgeneDDX5

GO:0000381

regulation of alternative mRNA splicing, via spliceosome

21343338

HgeneDDX5

GO:0000956

nuclear-transcribed mRNA catabolic process

23788676

TgenePRKCB

GO:0010827

regulation of glucose transmembrane transport

25982116

TgenePRKCB

GO:0035408

histone H3-T6 phosphorylation

20228790


check buttonFusion gene breakpoints across DDX5 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across PRKCB (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/ACD683976DDX5chr17

62502192

-PRKCBchr16

24166004

+


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000225792DDX5chr1762502192-ENST00000321728PRKCBchr1624166004+18954464491396315
ENST00000225792DDX5chr1762502192-ENST00000303531PRKCBchr1624166004+71984464491402317
ENST00000450599DDX5chr1762502192-ENST00000321728PRKCBchr1624166004+16612122151162315
ENST00000450599DDX5chr1762502192-ENST00000303531PRKCBchr1624166004+69642122151168317

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000225792ENST00000321728DDX5chr1762502192-PRKCBchr1624166004+0.0006058810.99939406
ENST00000225792ENST00000303531DDX5chr1762502192-PRKCBchr1624166004+0.0003787080.9996213
ENST00000450599ENST00000321728DDX5chr1762502192-PRKCBchr1624166004+0.0004475130.9995524
ENST00000450599ENST00000303531DDX5chr1762502192-PRKCBchr1624166004+0.0003619770.99963796

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>22091_22091_1_DDX5-PRKCB_DDX5_chr17_62502192_ENST00000225792_PRKCB_chr16_24166004_ENST00000303531_length(amino acids)=317AA_BP=0
MLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFY
AAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLA
GQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNA

--------------------------------------------------------------

>22091_22091_2_DDX5-PRKCB_DDX5_chr17_62502192_ENST00000225792_PRKCB_chr16_24166004_ENST00000321728_length(amino acids)=315AA_BP=0
MLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFY
AAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLA
GQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRD

--------------------------------------------------------------

>22091_22091_3_DDX5-PRKCB_DDX5_chr17_62502192_ENST00000450599_PRKCB_chr16_24166004_ENST00000303531_length(amino acids)=317AA_BP=0
MLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFY
AAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLA
GQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNA

--------------------------------------------------------------

>22091_22091_4_DDX5-PRKCB_DDX5_chr17_62502192_ENST00000450599_PRKCB_chr16_24166004_ENST00000321728_length(amino acids)=315AA_BP=0
MLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFY
AAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLA
GQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRD

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:62502192/chr16:24166004)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
DDX5

Q5T1V6

.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgenePRKCBchr17:62502192chr16:24166004ENST00000303531817601_671355.0674.0DomainAGC-kinase C-terminal
TgenePRKCBchr17:62502192chr16:24166004ENST00000321728817601_671355.0672.0DomainAGC-kinase C-terminal
TgenePRKCBchr17:62502192chr16:24166004ENST0000049805802158_275058.0DomainC2
TgenePRKCBchr17:62502192chr16:24166004ENST0000049805802342_600058.0DomainProtein kinase
TgenePRKCBchr17:62502192chr16:24166004ENST0000049805802601_671058.0DomainAGC-kinase C-terminal
TgenePRKCBchr17:62502192chr16:24166004ENST0000049805802348_356058.0Nucleotide bindingATP
TgenePRKCBchr17:62502192chr16:24166004ENST0000049805802101_151058.0Zinc fingerPhorbol-ester/DAG-type 2
TgenePRKCBchr17:62502192chr16:24166004ENST000004980580236_86058.0Zinc fingerPhorbol-ester/DAG-type 1

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneDDX5chr17:62502192chr16:24166004ENST00000225792-113125_30014.666666666666666615.0DomainHelicase ATP-binding
HgeneDDX5chr17:62502192chr16:24166004ENST00000225792-113328_47514.666666666666666615.0DomainHelicase C-terminal
HgeneDDX5chr17:62502192chr16:24166004ENST00000225792-113248_25114.666666666666666615.0MotifNote=DEAD box
HgeneDDX5chr17:62502192chr16:24166004ENST00000225792-11394_12214.666666666666666615.0MotifNote=Q motif
HgeneDDX5chr17:62502192chr16:24166004ENST00000225792-113114_11614.666666666666666615.0Nucleotide bindingNote=ATP
HgeneDDX5chr17:62502192chr16:24166004ENST00000225792-113138_14514.666666666666666615.0Nucleotide bindingNote=ATP
HgeneDDX5chr17:62502192chr16:24166004ENST00000225792-113477_61414.666666666666666615.0RegionNote=Transactivation domain
TgenePRKCBchr17:62502192chr16:24166004ENST00000303531817158_275355.0674.0DomainC2
TgenePRKCBchr17:62502192chr16:24166004ENST00000303531817342_600355.0674.0DomainProtein kinase
TgenePRKCBchr17:62502192chr16:24166004ENST00000321728817158_275355.0672.0DomainC2
TgenePRKCBchr17:62502192chr16:24166004ENST00000321728817342_600355.0672.0DomainProtein kinase
TgenePRKCBchr17:62502192chr16:24166004ENST00000303531817348_356355.0674.0Nucleotide bindingATP
TgenePRKCBchr17:62502192chr16:24166004ENST00000321728817348_356355.0672.0Nucleotide bindingATP
TgenePRKCBchr17:62502192chr16:24166004ENST00000303531817101_151355.0674.0Zinc fingerPhorbol-ester/DAG-type 2
TgenePRKCBchr17:62502192chr16:24166004ENST0000030353181736_86355.0674.0Zinc fingerPhorbol-ester/DAG-type 1
TgenePRKCBchr17:62502192chr16:24166004ENST00000321728817101_151355.0672.0Zinc fingerPhorbol-ester/DAG-type 2
TgenePRKCBchr17:62502192chr16:24166004ENST0000032172881736_86355.0672.0Zinc fingerPhorbol-ester/DAG-type 1


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
DDX5STK24, HDAC1, CREBBP, EP300, AKAP8, FBL, OTUB1, MEPCE, CDK9, HNRNPA1, HNRNPH1, KHDRBS2, IPO11, TCEA1, AIRE, MYOD1, SMARCA4, TBP, POLR2A, MGMT, SRRM1, SRRM2, tat, PIAS1, MBD3, CHD3, ESR1, TADA2A, SMN1, CD4, DGCR8, ZWINT, Cdk1, AI837181, Ybx1, CALM1, EBNA-LP, PIK3CA, PSMA3, TNNT1, VHL, SMARCAD1, YY1, NDRG1, HDGF, CENPA, MBNL1, SF3A2, ARRB2, ERG, NFX1, CUL3, CUL4A, CUL4B, CUL5, CUL2, CUL1, COPS5, COPS6, DCUN1D1, CAND1, NEDD8, DDX5, DDX17, HDAC2, HDAC3, BBLF2-BBLF3, INPPL1, SHC1, CRK, AKT1, DHX15, SRSF1, SF3B3, U2AF2, DHX38, SNRPA1, EIF4A3, PRKDC, SF3A1, HNRNPM, HNRNPF, SNRPD1, RBMX, SYNCRIP, U2AF1, SART1, PRPF40A, IGF2BP3, SNW1, PRPF19, SNRPD2, VTN, MCM7, SYN1, RPS20, XRCC4, RPS16, HNRNPA0, EEF2, PRSS1, THRAP3, GOLIM4, EPRS, RPL19, SRP14, SMURF1, FN1, VCAM1, RBM10, WBP11, HNRNPK, DHX9, RBM4, HNRNPH2, HNRNPH3, KHDRBS1, RBFOX2, IL7R, MAP1LC3A, ITGA4, CTNNB1, CD81, IGSF8, FBXW4, FBXO46, SP1, MAPK10, FBXO6, TARDBP, PARK2, PIN1, DBNL, ERO1L, FKBP10, LRSAM1, PABPC4, PRPSAP1, WDR4, rev, RPA1, RPA2, RPA3, STAU1, MDM2, SPRTN, AURKA, AURKB, CEP250, TP53, TUBGCP3, FUS, MAPKAPK2, DROSHA, NXF1, CUL7, OBSL1, CCDC8, UBE2I, EZH2, SUZ12, EED, RNF2, SMAD3, ABL1, ARAF, CDK2, DAPK1, ILK, MAST3, SGK2, SRPK1, TNK1, TYK2, LUZP4, RPS6KB2, UNK, NTRK1, ATXN2, ATXN2L, C14orf166, CPSF6, DDX18, DNAJC2, ELAC2, GTPBP4, HNRNPR, IMPDH1, SBDS, SKIV2L2, TRMT6, DHX36, EDC4, EIF2A, GMPS, LUC7L2, NACA, UPF1, SFN, SCARNA22, FRS3, gag, XPO1, HNRNPU, RPL10, MATR3, Bcas2, Bag2, Fus, SKI, MCM2, MCM5, Ksr1, CDC5L, CDC37, CRBN, CBX7, RYBP, WWP2, DERL1, EGFR, YPEL5, RMND5A, MAEA, RANBP9, ARMC8, NR2E1, CYLD, CD2BP2, INO80B, TRIM25, HOTAIR, HEY1, BRCA1, LMNA, MTF1, FBXO7, IQGAP1, TMPO, WDR77, MTCH2, PCBP1, PPIE, CCNT1, GSK3A, MAP2K3, MED12, POLD1, TGFB1, TRIP4, ZNF217, PRPF8, EFTUD2, AAR2, PIH1D1, TNIP2, CHD4, LARP7, RNF4, TNF, RIOK1, SQSTM1, HEXIM1, RUNX1, DPF2, RNF123, NBN, SNAI1, AGR2, RECQL4, ZFP36L2, CNTNAP1, MYC, Prkaa1, Prkab1, CDK5RAP2, METTL3, METTL14, WTAP, KIAA1429, PSME3, PHF1, PHB, FAF1, RBX1, KIN, USP14, NR2C2, UCHL3, PPP1CC, SETD1A, ZFYVE21, XRCC6, CTCF, AGRN, VRK1, HIST1H4A, PSMD14, APEX1, SNRNP70, EWSR1, TAF15, SNRPC, ITFG1, GSK3B, CDC6, BIRC3, BRD7, ERCC6, SOX2, TRIM28, CMTR1, PLEKHA4, MAGEA3, KCTD10, PINK1, YAP1, TFCP2, FANCD2, ORF50, ZC3H18, PTK6, STAG2, MAU2, HSCB, CELF1, ELAVL1, DUX4L9, DUX4, CIT, ANLN, CHMP4B, CHMP4C, ECT2, KIF14, KIF20A, KIF23, PRC1, ACACA, C12orf49, INS, SUMO2, NDN, BRD4, NUPR1, RBM45, CIC, Apc2, RBM39, FBP1, nsp13ab, LGALS9, IFI16, EIF3F, DDX58, OGT, SPOP, UFL1, DDRGK1, NR4A1, DDX23, NIFK, RPL31, SLC26A4-AS1, E2F1, FZR1, WDR5, PAGE4, NAA40, BTF3, AGO2, AGO1, RCHY1, NBR1, nsp14, RB1CC1, DDX3X, ZEB1,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
DDX5all structure
PRKCB


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to DDX5-PRKCB


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to DDX5-PRKCB


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneDDX5C0014175Endometriosis1CTD_human
HgeneDDX5C0269102Endometrioma1CTD_human