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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:FBXO31-ZNRF1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: FBXO31-ZNRF1
	FusionPDB ID: 29795
	FusionGDB2.0 ID: 29795
		Hgene	Tgene
	Gene symbol	FBXO31	ZNRF1
	Gene ID	79791	84937
	Gene name	F-box protein 31	zinc and ring finger 1
	Synonyms	FBX14\|FBXO14\|Fbx31\|MRT45\|pp2386	NIN283
	Cytomap	16q24.2	16q23.1
	Type of gene	protein-coding	protein-coding
	Description	F-box only protein 31SCF ubiquitin ligase specificity factorputative breast cancer tumor-suppressor	E3 ubiquitin-protein ligase ZNRF1RING-type E3 ubiquitin transferase ZNRF1nerve injury gene 283nerve injury-induced gene 283 proteinzinc and ring finger 1, E3 ubiquitin protein ligasezinc and ring finger protein 1zinc/RING finger protein 1
	Modification date	20200313	20200313
	UniProtAcc	Q5XUX0	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000311635, ENST00000563956,	ENST00000564320, ENST00000320619, ENST00000335325, ENST00000566250, ENST00000567962,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	4 X 4 X 4=64	7 X 7 X 6=294
	# samples	6	9
	** MAII score	log2(6/64*10)=-0.0931094043914815 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/294*10)=-1.70781924850669 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: FBXO31 [Title/Abstract] AND ZNRF1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	FBXO31(87393901)-ZNRF1(75127470), # samples:3
Anticipated loss of major functional domain due to fusion event.	FBXO31-ZNRF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FBXO31-ZNRF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FBXO31-ZNRF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. FBXO31-ZNRF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	FBXO31	GO:0006974	cellular response to DNA damage stimulus	19412162
Hgene	FBXO31	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	19412162

Fusion gene breakpoints across FBXO31 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ZNRF1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BLCA	TCGA-E5-A4TZ-01A	FBXO31	chr16	87393901	-	ZNRF1	chr16	75127470	+
ChimerDB4	BLCA	TCGA-E5-A4TZ	FBXO31	chr16	87393900	-	ZNRF1	chr16	75127469	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000311635	FBXO31	chr16	87393901	-	ENST00000335325	ZNRF1	chr16	75127470	+	3979	425	4	684	226
ENST00000311635	FBXO31	chr16	87393901	-	ENST00000320619	ZNRF1	chr16	75127470	+	1845	425	4	837	277
ENST00000311635	FBXO31	chr16	87393901	-	ENST00000566250	ZNRF1	chr16	75127470	+	743	425	4	630	208
ENST00000311635	FBXO31	chr16	87393901	-	ENST00000567962	ZNRF1	chr16	75127470	+	908	425	4	684	226
ENST00000311635	FBXO31	chr16	87393900	-	ENST00000335325	ZNRF1	chr16	75127469	+	3979	425	4	684	226
ENST00000311635	FBXO31	chr16	87393900	-	ENST00000320619	ZNRF1	chr16	75127469	+	1845	425	4	837	277
ENST00000311635	FBXO31	chr16	87393900	-	ENST00000566250	ZNRF1	chr16	75127469	+	743	425	4	630	208
ENST00000311635	FBXO31	chr16	87393900	-	ENST00000567962	ZNRF1	chr16	75127469	+	908	425	4	684	226

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000311635	ENST00000335325	FBXO31	chr16	87393901	-	ZNRF1	chr16	75127470	+	0.0491172	0.9508828
ENST00000311635	ENST00000320619	FBXO31	chr16	87393901	-	ZNRF1	chr16	75127470	+	0.09607743	0.9039226
ENST00000311635	ENST00000566250	FBXO31	chr16	87393901	-	ZNRF1	chr16	75127470	+	0.029749556	0.9702505
ENST00000311635	ENST00000567962	FBXO31	chr16	87393901	-	ZNRF1	chr16	75127470	+	0.025009606	0.9749904
ENST00000311635	ENST00000335325	FBXO31	chr16	87393900	-	ZNRF1	chr16	75127469	+	0.0491172	0.9508828
ENST00000311635	ENST00000320619	FBXO31	chr16	87393900	-	ZNRF1	chr16	75127469	+	0.09607743	0.9039226
ENST00000311635	ENST00000566250	FBXO31	chr16	87393900	-	ZNRF1	chr16	75127469	+	0.029749556	0.9702505
ENST00000311635	ENST00000567962	FBXO31	chr16	87393900	-	ZNRF1	chr16	75127469	+	0.025009606	0.9749904

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>29795_29795_1_FBXO31-ZNRF1_FBXO31_chr16_87393900_ENST00000311635_ZNRF1_chr16_75127469_ENST00000320619_length(amino acids)=277AA_BP=141
MGGMAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDL
PSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKRFKCPICSKSVASDEMEMHFIMCLSKPRLSYNGRIQRSLR
GAQPEKTWLSGRTLQEQPWRTECSWAPMAQMQSYPHCPVENREDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSCIDSWFEVNRS

--------------------------------------------------------------

>29795_29795_2_FBXO31-ZNRF1_FBXO31_chr16_87393900_ENST00000311635_ZNRF1_chr16_75127469_ENST00000335325_length(amino acids)=226AA_BP=141
MGGMAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDL
PSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKRFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDA

--------------------------------------------------------------

>29795_29795_3_FBXO31-ZNRF1_FBXO31_chr16_87393900_ENST00000311635_ZNRF1_chr16_75127469_ENST00000566250_length(amino acids)=208AA_BP=141
MGGMAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDL
PSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKRFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDA

--------------------------------------------------------------

>29795_29795_4_FBXO31-ZNRF1_FBXO31_chr16_87393900_ENST00000311635_ZNRF1_chr16_75127469_ENST00000567962_length(amino acids)=226AA_BP=141
MGGMAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDL
PSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKRFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDA

--------------------------------------------------------------

>29795_29795_5_FBXO31-ZNRF1_FBXO31_chr16_87393901_ENST00000311635_ZNRF1_chr16_75127470_ENST00000320619_length(amino acids)=277AA_BP=141
MGGMAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDL
PSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKRFKCPICSKSVASDEMEMHFIMCLSKPRLSYNGRIQRSLR
GAQPEKTWLSGRTLQEQPWRTECSWAPMAQMQSYPHCPVENREDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSCIDSWFEVNRS

--------------------------------------------------------------

>29795_29795_6_FBXO31-ZNRF1_FBXO31_chr16_87393901_ENST00000311635_ZNRF1_chr16_75127470_ENST00000335325_length(amino acids)=226AA_BP=141
MGGMAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDL
PSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKRFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDA

--------------------------------------------------------------

>29795_29795_7_FBXO31-ZNRF1_FBXO31_chr16_87393901_ENST00000311635_ZNRF1_chr16_75127470_ENST00000566250_length(amino acids)=208AA_BP=141
MGGMAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDL
PSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKRFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDA

--------------------------------------------------------------

>29795_29795_8_FBXO31-ZNRF1_FBXO31_chr16_87393901_ENST00000311635_ZNRF1_chr16_75127470_ENST00000567962_length(amino acids)=226AA_BP=141
MGGMAVCARLCGVGPSRGCRRRQQRRGPAETAAADSEPDTDPEEERIEASAGVGGGLCAGPSPPPPRCSLLELPPELLVEIFASLPGTDL
PSLAQVCTKFRRILHTDTIWRRRCREEYGVCENLRKLEITGVSCRDVYAKRFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDA

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr16:87393901/chr16:75127470)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
FBXO31 Q5XUX0	.
FUNCTION: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor. {ECO:0000269\|PubMed:16357137, ECO:0000269\|PubMed:19412162}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

FBXO31

chr16:87393900

chr16:75127469

ENST00000311635

58_63

137.33333333333334

540.0

Compositional bias

Note=Poly-Pro

Hgene

FBXO31

chr16:87393901

chr16:75127470

ENST00000311635

58_63

137.33333333333334

540.0

Compositional bias

Note=Poly-Pro

Hgene

FBXO31

chr16:87393900

chr16:75127469

ENST00000311635

64_110

137.33333333333334

540.0

Domain

F-box

Hgene

FBXO31

chr16:87393901

chr16:75127470

ENST00000311635

64_110

137.33333333333334

540.0

Domain

F-box

Tgene

ZNRF1

chr16:87393900

chr16:75127469

ENST00000320619

184_225

141.33333333333334

251.0

Zinc finger

RING-type%3B atypical

Tgene

ZNRF1

chr16:87393900

chr16:75127469

ENST00000335325

184_225

141.33333333333334

960.0

Zinc finger

RING-type%3B atypical

Tgene

ZNRF1

chr16:87393900

chr16:75127469

ENST00000567962

184_225

141.33333333333334

41.333333333333336

Zinc finger

RING-type%3B atypical

Tgene

ZNRF1

chr16:87393901

chr16:75127470

ENST00000320619

184_225

141.33333333333334

251.0

Zinc finger

RING-type%3B atypical

Tgene

ZNRF1

chr16:87393901

chr16:75127470

ENST00000335325

184_225

141.33333333333334

960.0

Zinc finger

RING-type%3B atypical

Tgene

ZNRF1

chr16:87393901

chr16:75127470

ENST00000567962

184_225

141.33333333333334

41.333333333333336

Zinc finger

RING-type%3B atypical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

ZNRF1

chr16:87393900

chr16:75127469

ENST00000320619

2_10

141.33333333333334

251.0

Region

Note=Required for endosomal and lysosomal localization and myristoylation

Tgene

ZNRF1

chr16:87393900

chr16:75127469

ENST00000335325

2_10

141.33333333333334

960.0

Region

Note=Required for endosomal and lysosomal localization and myristoylation

Tgene

ZNRF1

chr16:87393900

chr16:75127469

ENST00000567962

2_10

141.33333333333334

41.333333333333336

Region

Note=Required for endosomal and lysosomal localization and myristoylation

Tgene

ZNRF1

chr16:87393901

chr16:75127470

ENST00000320619

2_10

141.33333333333334

251.0

Region

Note=Required for endosomal and lysosomal localization and myristoylation

Tgene

ZNRF1

chr16:87393901

chr16:75127470

ENST00000335325

2_10

141.33333333333334

960.0

Region

Note=Required for endosomal and lysosomal localization and myristoylation

Tgene

ZNRF1

chr16:87393901

chr16:75127470

ENST00000567962

2_10

141.33333333333334

41.333333333333336

Region

Note=Required for endosomal and lysosomal localization and myristoylation

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
FBXO31
ZNRF1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to FBXO31-ZNRF1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to FBXO31-ZNRF1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source