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Fusion Protein:G2E3-NAP1L1 |
Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: G2E3-NAP1L1 | FusionPDB ID: 31983 | FusionGDB2.0 ID: 31983 | Hgene | Tgene | Gene symbol | G2E3 | NAP1L1 | Gene ID | 55632 | 4673 |
Gene name | G2/M-phase specific E3 ubiquitin protein ligase | nucleosome assembly protein 1 like 1 | |
Synonyms | KIAA1333|PHF7B | NAP1|NAP1L|NRP | |
Cytomap | 14q12 | 12q21.2 | |
Type of gene | protein-coding | protein-coding | |
Description | G2/M phase-specific E3 ubiquitin-protein ligaseG2/M phase-specific HECT-type E3 ubiquitin transferaseG2/M-phase specific E3 ubiquitin ligasePHD finger protein 7B | nucleosome assembly protein 1-like 1HSP22-like protein interacting proteinNAP-1-related protein | |
Modification date | 20200313 | 20200322 | |
UniProtAcc | Q7L622 | P55209 | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000544007, ENST00000206595, ENST00000438909, ENST00000553504, | ENST00000261182, ENST00000393263, ENST00000431879, ENST00000535020, ENST00000542344, ENST00000544816, ENST00000547773, ENST00000547993, ENST00000548044, ENST00000549596, ENST00000552342, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 7 X 4 X 5=140 | 18 X 20 X 6=2160 |
# samples | 7 | 22 | |
** MAII score | log2(7/140*10)=-1 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(22/2160*10)=-3.29545588352617 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Context (manual curation of fusion genes in FusionPDB) | PubMed: G2E3 [Title/Abstract] AND NAP1L1 [Title/Abstract] AND fusion [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | G2E3(31050322)-NAP1L1(76443565), # samples:3 | ||
Anticipated loss of major functional domain due to fusion event. | G2E3-NAP1L1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. G2E3-NAP1L1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. G2E3-NAP1L1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. G2E3-NAP1L1 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. G2E3-NAP1L1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
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![]() * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
ChimerDB4 | LUAD | TCGA-69-7760-01A | G2E3 | chr14 | 31028478 | + | NAP1L1 | chr12 | 76443565 | - |
ChimerDB4 | LUAD | TCGA-69-7760-01A | G2E3 | chr14 | 31050322 | + | NAP1L1 | chr12 | 76443565 | - |
ChimerDB4 | LUAD | TCGA-69-7760 | G2E3 | chr14 | 31050322 | + | NAP1L1 | chr12 | 76443565 | - |
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Fusion ORF Analysis |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000206595 | G2E3 | chr14 | 31050322 | + | ENST00000552342 | NAP1L1 | chr12 | 76443565 | - | 512 | 191 | 46 | 195 | 49 |
ENST00000553504 | G2E3 | chr14 | 31050322 | + | ENST00000552342 | NAP1L1 | chr12 | 76443565 | - | 1895 | 1574 | 129 | 401 | 90 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000206595 | ENST00000552342 | G2E3 | chr14 | 31050322 | + | NAP1L1 | chr12 | 76443565 | - | 0.81425256 | 0.18574744 |
ENST00000553504 | ENST00000552342 | G2E3 | chr14 | 31050322 | + | NAP1L1 | chr12 | 76443565 | - | 0.76221687 | 0.23778318 |
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Fusion Amino Acid Sequences |
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>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >31983_31983_1_G2E3-NAP1L1_G2E3_chr14_31050322_ENST00000206595_NAP1L1_chr12_76443565_ENST00000552342_length(amino acids)=49AA_BP= -------------------------------------------------------------- >31983_31983_2_G2E3-NAP1L1_G2E3_chr14_31050322_ENST00000553504_NAP1L1_chr12_76443565_ENST00000552342_length(amino acids)=90AA_BP= MGAFAGPALSTVAHASQLSVLRIRRPCRSPASKPCLYASSVAFFKPVVYYFHQFGSNAEILTVVKSHCEGVATFKQISFNERWKVGAGLE -------------------------------------------------------------- |
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Fusion Protein Functional Features |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr14:31050322/chr12:76443565) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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Hgene | Tgene |
G2E3 | NAP1L1 |
FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death. {ECO:0000269|PubMed:18511420}. | FUNCTION: Histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly (PubMed:20002496, PubMed:26841755). Participates also in several important DNA repair mechanisms: greatly enhances ERCC6-mediated chromatin remodeling which is essential for transcription-coupled nucleotide excision DNA repair (PubMed:28369616). Stimulates also homologous recombination (HR) by RAD51 and RAD54 which is essential in mitotic DNA double strand break (DSB) repair (PubMed:24798879). Plays a key role in the regulation of embryonic neurogenesis (By similarity). Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development (By similarity). Regulates neurogenesis via the modulation of RASSF10; regulates RASSF10 expression by promoting SETD1A-mediated H3K4 methylation at the RASSF10 promoter (By similarity). {ECO:0000250|UniProtKB:P28656, ECO:0000269|PubMed:20002496, ECO:0000269|PubMed:24798879, ECO:0000269|PubMed:26841755, ECO:0000269|PubMed:28369616}.; FUNCTION: (Microbial infection) Positively regulates Epstein-Barr virus reactivation in epithelial cells through the induction of viral BZLF1 expression. {ECO:0000269|PubMed:23691099}.; FUNCTION: (Microbial infection) Together with human herpesvirus 8 protein LANA1, assists the proper assembly of the nucleosome on the replicated viral DNA. {ECO:0000269|PubMed:27599637}. |
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- Retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
- Not-retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
Hgene | G2E3 | chr14:31050322 | chr12:76443565 | ENST00000206595 | + | 2 | 15 | 371_698 | 12.333333333333334 | 707.0 | Domain | HECT |
Hgene | G2E3 | chr14:31050322 | chr12:76443565 | ENST00000206595 | + | 2 | 15 | 11_51 | 12.333333333333334 | 707.0 | Zinc finger | C2HC pre-PHD-type |
Hgene | G2E3 | chr14:31050322 | chr12:76443565 | ENST00000206595 | + | 2 | 15 | 143_193 | 12.333333333333334 | 707.0 | Zinc finger | Note=PHD-type 2%3B degenerate |
Hgene | G2E3 | chr14:31050322 | chr12:76443565 | ENST00000206595 | + | 2 | 15 | 237_286 | 12.333333333333334 | 707.0 | Zinc finger | Note=PHD-type 3 |
Hgene | G2E3 | chr14:31050322 | chr12:76443565 | ENST00000206595 | + | 2 | 15 | 79_128 | 12.333333333333334 | 707.0 | Zinc finger | PHD-type 1 |
Tgene | NAP1L1 | chr14:31050322 | chr12:76443565 | ENST00000261182 | 11 | 15 | 10_30 | 353.0 | 392.0 | Compositional bias | Note=Asp/Glu-rich (acidic) | |
Tgene | NAP1L1 | chr14:31050322 | chr12:76443565 | ENST00000261182 | 11 | 15 | 129_145 | 353.0 | 392.0 | Compositional bias | Note=Asp/Glu-rich (acidic) | |
Tgene | NAP1L1 | chr14:31050322 | chr12:76443565 | ENST00000261182 | 11 | 15 | 348_378 | 353.0 | 392.0 | Compositional bias | Note=Asp/Glu-rich (acidic) | |
Tgene | NAP1L1 | chr14:31050322 | chr12:76443565 | ENST00000393263 | 11 | 16 | 10_30 | 353.0 | 558.3333333333334 | Compositional bias | Note=Asp/Glu-rich (acidic) | |
Tgene | NAP1L1 | chr14:31050322 | chr12:76443565 | ENST00000393263 | 11 | 16 | 129_145 | 353.0 | 558.3333333333334 | Compositional bias | Note=Asp/Glu-rich (acidic) | |
Tgene | NAP1L1 | chr14:31050322 | chr12:76443565 | ENST00000393263 | 11 | 16 | 348_378 | 353.0 | 558.3333333333334 | Compositional bias | Note=Asp/Glu-rich (acidic) | |
Tgene | NAP1L1 | chr14:31050322 | chr12:76443565 | ENST00000261182 | 11 | 15 | 273_279 | 353.0 | 392.0 | Motif | Nuclear localization signal | |
Tgene | NAP1L1 | chr14:31050322 | chr12:76443565 | ENST00000393263 | 11 | 16 | 273_279 | 353.0 | 558.3333333333334 | Motif | Nuclear localization signal |
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Fusion Protein Structures |
![]() * Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
Fusion protein PDB link (fusion AA seq ID in FusionPDB) | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | AA seq | Len(AA seq) |
PDB file >>>15_G2E3_31050322_NAP1L1_76443565_ranked_0.pdb | G2E3 | 31050322 | 31050322 | ENST00000552342 | NAP1L1 | chr12 | 76443565 | - | MGAFAGPALSTVAHASQLSVLRIRRPCRSPASKPCLYASSVAFFKPVVYYFHQFGSNAEILTVVKSHCEGVATFKQISFNERWKVGAGLE | 90 |
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pLDDT score distribution |
![]() * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
G2E3_pLDDT.png![]() |
![]() * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
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Ramachandran Plot of Fusion Protein Structure |
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Fusion AA seq ID in FusionPDB and their Ramachandran plots |
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Fusion Protein-Protein Interaction |
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Gene | PPI interactors |
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Gene | STRING network |
G2E3 | |
NAP1L1 |
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Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
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Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to G2E3-NAP1L1 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to G2E3-NAP1L1 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |