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Center for Computational Systems Medicine level2
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:GRAMD4-TBC1D22A

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: GRAMD4-TBC1D22A
FusionPDB ID: 34605
FusionGDB2.0 ID: 34605
HgeneTgene
Gene symbol

GRAMD4

TBC1D22A

Gene ID

23151

25771

Gene nameGRAM domain containing 4TBC1 domain family member 22A
SynonymsDIPC22orf4|HSC79E021
Cytomap

22q13.31

22q13.31

Type of geneprotein-codingprotein-coding
DescriptionGRAM domain-containing protein 4death-inducing-proteinTBC1 domain family member 22Aputative GTPase activator
Modification date2020031320200313
UniProtAcc

Q6IC98

.
Ensembl transtripts involved in fusion geneENST idsENST00000361034, ENST00000406902, 
ENST00000490378, ENST00000408031, 
ENST00000355704, ENST00000380995, 
ENST00000406733, ENST00000407381, 
ENST00000472791, ENST00000337137, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score16 X 11 X 7=123222 X 24 X 11=5808
# samples 2232
** MAII scorelog2(22/1232*10)=-2.48542682717024
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(32/5808*10)=-4.18189764310839
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: GRAMD4 [Title/Abstract] AND TBC1D22A [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)GRAMD4(47033857)-TBC1D22A(47274549), # samples:2
TBC1D22A(47193517)-GRAMD4(47022648), # samples:1
TBC1D22A(47308084)-GRAMD4(47022648), # samples:1
Anticipated loss of major functional domain due to fusion event.GRAMD4-TBC1D22A seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
GRAMD4-TBC1D22A seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
GRAMD4-TBC1D22A seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
GRAMD4-TBC1D22A seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
GRAMD4-TBC1D22A seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
GRAMD4-TBC1D22A seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across GRAMD4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across TBC1D22A (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-OL-A66I-01AGRAMD4chr22

47033857

+TBC1D22Achr22

47274549

+
ChimerDB4STADTCGA-BR-8372-01AGRAMD4chr22

47033857

+TBC1D22Achr22

47274549

+
ChimerDB4STADTCGA-BR-8372-01AGRAMD4chr22

47060014

+TBC1D22Achr22

47274549

+
ChimerDB4STADTCGA-CD-A487-01AGRAMD4chr22

47069711

+TBC1D22Achr22

47432967

+
ChimerDB4STADTCGA-CD-A487GRAMD4chr22

47069711

+TBC1D22Achr22

47432966

+
ChimerDB4STADTCGA-CG-4441-01AGRAMD4chr22

47022858

+TBC1D22Achr22

47370186

+
ChimerDB4STADTCGA-CG-4441-01AGRAMD4chr22

47022858

+TBC1D22Achr22

47370188

+
ChimerDB4STADTCGA-CG-4441-01AGRAMD4chr22

47022858

+TBC1D22Achr22

47393530

+
ChimerDB4STADTCGA-HU-A4GH-01AGRAMD4chr22

47033857

+TBC1D22Achr22

47393530

+
ChimerDB4STADTCGA-HU-A4GHGRAMD4chr22

47033857

+TBC1D22Achr22

47393529

+


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000406902GRAMD4chr2247069711+ENST00000337137TBC1D22Achr2247432966+401715972071949580
ENST00000361034GRAMD4chr2247069711+ENST00000337137TBC1D22Achr2247432966+38431423331775580
ENST00000406902GRAMD4chr2247033857+ENST00000337137TBC1D22Achr2247274549+34804962071412401
ENST00000406902GRAMD4chr2247033857+ENST00000380995TBC1D22Achr2247274549+975496207974255
ENST00000406902GRAMD4chr2247033857+ENST00000407381TBC1D22Achr2247274549+18704962071412401
ENST00000406902GRAMD4chr2247033857+ENST00000355704TBC1D22Achr2247274549+18734962071412401
ENST00000361034GRAMD4chr2247033857+ENST00000337137TBC1D22Achr2247274549+3306322331238401
ENST00000361034GRAMD4chr2247033857+ENST00000380995TBC1D22Achr2247274549+80132233800255
ENST00000361034GRAMD4chr2247033857+ENST00000407381TBC1D22Achr2247274549+1696322331238401
ENST00000361034GRAMD4chr2247033857+ENST00000355704TBC1D22Achr2247274549+1699322331238401
ENST00000490378GRAMD4chr2247033857+ENST00000337137TBC1D22Achr2247274549+31771932071109300
ENST00000490378GRAMD4chr2247033857+ENST00000380995TBC1D22Achr2247274549+672193207671154
ENST00000490378GRAMD4chr2247033857+ENST00000407381TBC1D22Achr2247274549+15671932071109300
ENST00000490378GRAMD4chr2247033857+ENST00000355704TBC1D22Achr2247274549+15701932071109300
ENST00000406902GRAMD4chr2247022858+ENST00000337137TBC1D22Achr2247393530+287137520761312254
ENST00000361034GRAMD4chr2247022858+ENST00000337137TBC1D22Achr2247393530+269720119021138254
ENST00000406902GRAMD4chr2247069711+ENST00000337137TBC1D22Achr2247432967+401715972071949580
ENST00000361034GRAMD4chr2247069711+ENST00000337137TBC1D22Achr2247432967+38431423331775580

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000406902ENST00000337137GRAMD4chr2247069711+TBC1D22Achr2247432966+0.0055575670.9944424
ENST00000361034ENST00000337137GRAMD4chr2247069711+TBC1D22Achr2247432966+0.0053680930.9946319
ENST00000406902ENST00000337137GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0017821510.9982179
ENST00000406902ENST00000380995GRAMD4chr2247033857+TBC1D22Achr2247274549+0.004674580.99532545
ENST00000406902ENST00000407381GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0028479550.997152
ENST00000406902ENST00000355704GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0028493890.9971506
ENST00000361034ENST00000337137GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0017324570.99826753
ENST00000361034ENST00000380995GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0047337780.9952662
ENST00000361034ENST00000407381GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0030823070.9969177
ENST00000361034ENST00000355704GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0030883520.9969117
ENST00000490378ENST00000337137GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0028867290.9971132
ENST00000490378ENST00000380995GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0085497130.99145025
ENST00000490378ENST00000407381GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0037443910.99625564
ENST00000490378ENST00000355704GRAMD4chr2247033857+TBC1D22Achr2247274549+0.0037746080.99622536
ENST00000406902ENST00000337137GRAMD4chr2247022858+TBC1D22Achr2247393530+0.0049314980.99506855
ENST00000361034ENST00000337137GRAMD4chr2247022858+TBC1D22Achr2247393530+0.0044766260.99552333
ENST00000406902ENST00000337137GRAMD4chr2247069711+TBC1D22Achr2247432967+0.0055575670.9944424
ENST00000361034ENST00000337137GRAMD4chr2247069711+TBC1D22Achr2247432967+0.0053680930.9946319

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>34605_34605_1_GRAMD4-TBC1D22A_GRAMD4_chr22_47022858_ENST00000361034_TBC1D22A_chr22_47393530_ENST00000337137_length(amino acids)=254AA_BP=56
MGSQSQGPWSQGHSPSRALGRDQLWALPHLEPQGTSPQREPVQGRTSIQRELVHGGNQSTAEPVQGRSGPGQEWSMAEPVHGGTGPRRNR
SMEEPVHDGTSHTSPTPGRPGQGQQVPGSLQKLIPLPGCGEKSTVVQGFDDRDATPCPSASPTWPDSLGGPPGALAEAGAYLRGGATWPW

--------------------------------------------------------------

>34605_34605_2_GRAMD4-TBC1D22A_GRAMD4_chr22_47022858_ENST00000406902_TBC1D22A_chr22_47393530_ENST00000337137_length(amino acids)=254AA_BP=56
MGSQSQGPWSQGHSPSRALGRDQLWALPHLEPQGTSPQREPVQGRTSIQRELVHGGNQSTAEPVQGRSGPGQEWSMAEPVHGGTGPRRNR
SMEEPVHDGTSHTSPTPGRPGQGQQVPGSLQKLIPLPGCGEKSTVVQGFDDRDATPCPSASPTWPDSLGGPPGALAEAGAYLRGGATWPW

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>34605_34605_3_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000361034_TBC1D22A_chr22_47274549_ENST00000337137_length(amino acids)=401AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP
KVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQ
MKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQE

--------------------------------------------------------------

>34605_34605_4_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000361034_TBC1D22A_chr22_47274549_ENST00000355704_length(amino acids)=401AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP
KVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQ
MKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQE

--------------------------------------------------------------

>34605_34605_5_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000361034_TBC1D22A_chr22_47274549_ENST00000380995_length(amino acids)=255AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP

--------------------------------------------------------------

>34605_34605_6_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000361034_TBC1D22A_chr22_47274549_ENST00000407381_length(amino acids)=401AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP
KVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQ
MKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQE

--------------------------------------------------------------

>34605_34605_7_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000406902_TBC1D22A_chr22_47274549_ENST00000337137_length(amino acids)=401AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP
KVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQ
MKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQE

--------------------------------------------------------------

>34605_34605_8_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000406902_TBC1D22A_chr22_47274549_ENST00000355704_length(amino acids)=401AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP
KVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQ
MKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQE

--------------------------------------------------------------

>34605_34605_9_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000406902_TBC1D22A_chr22_47274549_ENST00000380995_length(amino acids)=255AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP

--------------------------------------------------------------

>34605_34605_10_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000406902_TBC1D22A_chr22_47274549_ENST00000407381_length(amino acids)=401AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP
KVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQ
MKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQE

--------------------------------------------------------------

>34605_34605_11_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000490378_TBC1D22A_chr22_47274549_ENST00000337137_length(amino acids)=300AA_BP=
MSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVTEIFERILF
IWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQMKVKMLEELVS
RIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQELLLFLQNLPTA

--------------------------------------------------------------

>34605_34605_12_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000490378_TBC1D22A_chr22_47274549_ENST00000355704_length(amino acids)=300AA_BP=
MSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVTEIFERILF
IWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQMKVKMLEELVS
RIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQELLLFLQNLPTA

--------------------------------------------------------------

>34605_34605_13_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000490378_TBC1D22A_chr22_47274549_ENST00000380995_length(amino acids)=154AA_BP=
MSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVTEIFERILF

--------------------------------------------------------------

>34605_34605_14_GRAMD4-TBC1D22A_GRAMD4_chr22_47033857_ENST00000490378_TBC1D22A_chr22_47274549_ENST00000407381_length(amino acids)=300AA_BP=
MSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVTEIFERILF
IWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQMKVKMLEELVS
RIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQELLLFLQNLPTA

--------------------------------------------------------------

>34605_34605_15_GRAMD4-TBC1D22A_GRAMD4_chr22_47069711_ENST00000361034_TBC1D22A_chr22_47432966_ENST00000337137_length(amino acids)=580AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQEELRKLREETNAEMLRQELDRERQRRMELEQKVQEVLKARTEEQMAQQPPKGQAQASNGAERRSQGLSSRLQKWFYERFGEYV
EDFRFQPEENTVETEEPLSARRLTENMRRLKRGAKPVTNFVKNLSALSDWYSVYTSAIAFTVYMNAVWHGWAIPLFLFLAILRLSLNYLI
ARGWRIQWSIVPEVSEPVEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADILEKIKNLFMWVQPEITQKLYVALWAAFLASCFFPYRLVG
LAVGLYAGIKFFLIDFIFKRCPRLRAKYDTPYIIWRSLPTDPQLKERSSAAVSRRLQTTSSRSYVPSAPAGLGKEEDAGRFHSTKKGNFH
EIFNLTENERPLAEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQEL

--------------------------------------------------------------

>34605_34605_16_GRAMD4-TBC1D22A_GRAMD4_chr22_47069711_ENST00000361034_TBC1D22A_chr22_47432967_ENST00000337137_length(amino acids)=580AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQEELRKLREETNAEMLRQELDRERQRRMELEQKVQEVLKARTEEQMAQQPPKGQAQASNGAERRSQGLSSRLQKWFYERFGEYV
EDFRFQPEENTVETEEPLSARRLTENMRRLKRGAKPVTNFVKNLSALSDWYSVYTSAIAFTVYMNAVWHGWAIPLFLFLAILRLSLNYLI
ARGWRIQWSIVPEVSEPVEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADILEKIKNLFMWVQPEITQKLYVALWAAFLASCFFPYRLVG
LAVGLYAGIKFFLIDFIFKRCPRLRAKYDTPYIIWRSLPTDPQLKERSSAAVSRRLQTTSSRSYVPSAPAGLGKEEDAGRFHSTKKGNFH
EIFNLTENERPLAEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQEL

--------------------------------------------------------------

>34605_34605_17_GRAMD4-TBC1D22A_GRAMD4_chr22_47069711_ENST00000406902_TBC1D22A_chr22_47432966_ENST00000337137_length(amino acids)=580AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQEELRKLREETNAEMLRQELDRERQRRMELEQKVQEVLKARTEEQMAQQPPKGQAQASNGAERRSQGLSSRLQKWFYERFGEYV
EDFRFQPEENTVETEEPLSARRLTENMRRLKRGAKPVTNFVKNLSALSDWYSVYTSAIAFTVYMNAVWHGWAIPLFLFLAILRLSLNYLI
ARGWRIQWSIVPEVSEPVEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADILEKIKNLFMWVQPEITQKLYVALWAAFLASCFFPYRLVG
LAVGLYAGIKFFLIDFIFKRCPRLRAKYDTPYIIWRSLPTDPQLKERSSAAVSRRLQTTSSRSYVPSAPAGLGKEEDAGRFHSTKKGNFH
EIFNLTENERPLAEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQEL

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>34605_34605_18_GRAMD4-TBC1D22A_GRAMD4_chr22_47069711_ENST00000406902_TBC1D22A_chr22_47432967_ENST00000337137_length(amino acids)=580AA_BP=1
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQEELRKLREETNAEMLRQELDRERQRRMELEQKVQEVLKARTEEQMAQQPPKGQAQASNGAERRSQGLSSRLQKWFYERFGEYV
EDFRFQPEENTVETEEPLSARRLTENMRRLKRGAKPVTNFVKNLSALSDWYSVYTSAIAFTVYMNAVWHGWAIPLFLFLAILRLSLNYLI
ARGWRIQWSIVPEVSEPVEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADILEKIKNLFMWVQPEITQKLYVALWAAFLASCFFPYRLVG
LAVGLYAGIKFFLIDFIFKRCPRLRAKYDTPYIIWRSLPTDPQLKERSSAAVSRRLQTTSSRSYVPSAPAGLGKEEDAGRFHSTKKGNFH
EIFNLTENERPLAEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQEL

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr22:47033857/chr22:47274549)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
GRAMD4

Q6IC98

.
FUNCTION: Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53 (PubMed:15565177). Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53. Inhibits TLR9 response to nucelic acids and regulates TLR9-mediated innate immune response (By similarity). {ECO:0000250|UniProtKB:Q8CB44, ECO:0000269|PubMed:15565177}.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000361034+141883_143461.3333333333333579.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000406902+151983_143461.3333333333333579.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000361034+141883_143461.3333333333333579.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000406902+151983_143461.3333333333333579.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000361034+1418240_260461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000361034+1418334_354461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000361034+1418356_376461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000406902+1519240_260461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000406902+1519334_354461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000406902+1519356_376461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000361034+1418240_260461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000361034+1418334_354461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000361034+1418356_376461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000406902+1519240_260461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000406902+1519334_354461.3333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000406902+1519356_376461.3333333333333579.0TransmembraneHelical
TgeneTBC1D22Achr22:47022858chr22:47393530ENST0000038099509222_4460325.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47033857chr22:47274549ENST00000337137313222_446212.33333333333334518.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47033857chr22:47274549ENST00000355704111222_446134.33333333333334440.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47033857chr22:47274549ENST0000038099539222_446165.33333333333334325.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47069711chr22:47432966ENST0000038099509222_4460325.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47069711chr22:47432967ENST0000038099509222_4460325.0DomainRab-GAP TBC

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000361034+11883_14354.0579.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000406902+21983_14354.0579.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000408031+1483_1430102.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000361034+21883_14394.33333333333333579.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000406902+31983_14394.33333333333333579.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000408031+1483_1430102.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000408031+1483_1430102.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000408031+1483_1430102.0Coiled coilOntology_term=ECO:0000255
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000361034+118445_52354.0579.0DomainNote=GRAM
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000406902+219445_52354.0579.0DomainNote=GRAM
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000408031+14445_5230102.0DomainNote=GRAM
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000361034+218445_52394.33333333333333579.0DomainNote=GRAM
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000406902+319445_52394.33333333333333579.0DomainNote=GRAM
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000408031+14445_5230102.0DomainNote=GRAM
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000361034+1418445_523461.3333333333333579.0DomainNote=GRAM
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000406902+1519445_523461.3333333333333579.0DomainNote=GRAM
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000408031+14445_5230102.0DomainNote=GRAM
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000361034+1418445_523461.3333333333333579.0DomainNote=GRAM
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000406902+1519445_523461.3333333333333579.0DomainNote=GRAM
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000408031+14445_5230102.0DomainNote=GRAM
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000361034+118240_26054.0579.0TransmembraneHelical
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000361034+118334_35454.0579.0TransmembraneHelical
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000361034+118356_37654.0579.0TransmembraneHelical
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000406902+219240_26054.0579.0TransmembraneHelical
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000406902+219334_35454.0579.0TransmembraneHelical
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000406902+219356_37654.0579.0TransmembraneHelical
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000408031+14240_2600102.0TransmembraneHelical
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000408031+14334_3540102.0TransmembraneHelical
HgeneGRAMD4chr22:47022858chr22:47393530ENST00000408031+14356_3760102.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000361034+218240_26094.33333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000361034+218334_35494.33333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000361034+218356_37694.33333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000406902+319240_26094.33333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000406902+319334_35494.33333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000406902+319356_37694.33333333333333579.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000408031+14240_2600102.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000408031+14334_3540102.0TransmembraneHelical
HgeneGRAMD4chr22:47033857chr22:47274549ENST00000408031+14356_3760102.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000408031+14240_2600102.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000408031+14334_3540102.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432966ENST00000408031+14356_3760102.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000408031+14240_2600102.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000408031+14334_3540102.0TransmembraneHelical
HgeneGRAMD4chr22:47069711chr22:47432967ENST00000408031+14356_3760102.0TransmembraneHelical
TgeneTBC1D22Achr22:47022858chr22:47393530ENST00000337137813222_446375.0518.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47022858chr22:47393530ENST00000355704611222_446297.0440.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47069711chr22:47432966ENST00000337137913222_446400.3333333333333518.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47069711chr22:47432966ENST00000355704711222_446322.3333333333333440.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47069711chr22:47432967ENST00000337137913222_446400.3333333333333518.0DomainRab-GAP TBC
TgeneTBC1D22Achr22:47069711chr22:47432967ENST00000355704711222_446322.3333333333333440.0DomainRab-GAP TBC


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Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file >>>804_GRAMD4_47033857_TBC1D22A_47274549_ranked_0.pdbGRAMD44706971147033857ENST00000337137TBC1D22Achr2247274549+
MNMLRRLDKIRFRGHKRDDFLDLAESPNASDTECSDEIPLKVPRTSPRDSEELRDPAGPGTLIMATGVQDFNRTEFDRLNEIKGHLEIAL
LEKHFLQELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQP
KVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQ
MKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQE
401


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
GRAMD4_pLDDT.png
all structure
all structure
TBC1D22A_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
GRAMD4
TBC1D22A


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to GRAMD4-TBC1D22A


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to GRAMD4-TBC1D22A


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource