| UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY |
|
|||||||
|
Fusion Protein:ITGB3-DYNLRB1 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: ITGB3-DYNLRB1 | FusionPDB ID: 40526 | FusionGDB2.0 ID: 40526 | Hgene | Tgene | Gene symbol | ITGB3 | DYNLRB1 | Gene ID | 3690 | 83658 |
| Gene name | integrin subunit beta 3 | dynein light chain roadblock-type 1 | |
| Synonyms | BDPLT16|BDPLT2|CD61|GP3A|GPIIIa|GT | BITH|BLP|DNCL2A|DNLC2A|ROBLD1 | |
| Cytomap | 17q21.32 | 20q11.22 | |
| Type of gene | protein-coding | protein-coding | |
| Description | integrin beta-3antigen CD61integrin beta 3integrin beta chain, beta 3integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)platelet membrane glycoprotein IIIa | dynein light chain roadblock-type 1ROBL/LC7-like 1bithoraxoid-like proteindynein, cytoplasmic, light polypeptide 2Adynein-associated protein Km23roadblock domain-containing protein 1 | |
| Modification date | 20200329 | 20200320 | |
| UniProtAcc | Q13352 | Q9NP97 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000559488, ENST00000560629, ENST00000571680, ENST00000435993, | ENST00000417166, ENST00000480759, ENST00000300469, ENST00000374846, ENST00000357156, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 3 X 2 X 2=12 | 17 X 12 X 12=2448 |
| # samples | 4 | 23 | |
| ** MAII score | log2(4/12*10)=1.73696559416621 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | log2(23/2448*10)=-3.41189779174828 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: ITGB3 [Title/Abstract] AND DYNLRB1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | ITGB3(45331306)-DYNLRB1(33122431), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | ITGB3-DYNLRB1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ITGB3-DYNLRB1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ITGB3-DYNLRB1 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. ITGB3-DYNLRB1 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF. ITGB3-DYNLRB1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | ITGB3 | GO:0033627 | cell adhesion mediated by integrin | 12807887 |
| Hgene | ITGB3 | GO:0034446 | substrate adhesion-dependent cell spreading | 24658351 |
| Hgene | ITGB3 | GO:0099149 | regulation of postsynaptic neurotransmitter receptor internalization | 18549786 |
| Fusion gene breakpoints across ITGB3 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
 |
| Fusion gene breakpoints across DYNLRB1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
 |
Top |
Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | OV | TCGA-24-1103 | ITGB3 | chr17 | 45331306 | + | DYNLRB1 | chr20 | 33122431 | + |
Top |
Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000559488 | ITGB3 | chr17 | 45331306 | + | ENST00000357156 | DYNLRB1 | chr20 | 33122431 | + | 641 | 95 | 369 | 1 | 123 |
| ENST00000571680 | ITGB3 | chr17 | 45331306 | + | ENST00000357156 | DYNLRB1 | chr20 | 33122431 | + | 634 | 88 | 362 | 0 | 121 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000559488 | ENST00000357156 | ITGB3 | chr17 | 45331306 | + | DYNLRB1 | chr20 | 33122431 | + | 0.1437805 | 0.8562195 |
| ENST00000571680 | ENST00000357156 | ITGB3 | chr17 | 45331306 | + | DYNLRB1 | chr20 | 33122431 | + | 0.13756448 | 0.86243546 |
Top |
Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >40526_40526_1_ITGB3-DYNLRB1_ITGB3_chr17_45331306_ENST00000559488_DYNLRB1_chr20_33122431_ENST00000357156_length(amino acids)=123AA_BP=1 MTLTFLGGIKLRNDTGSQESGLFGWILNHNQEIVFIWCNHNFIFLGANSKEGEIVLGVDVTHGAPCLQDEAVHEAGILGGGGVVHGALDG -------------------------------------------------------------- >40526_40526_2_ITGB3-DYNLRB1_ITGB3_chr17_45331306_ENST00000571680_DYNLRB1_chr20_33122431_ENST00000357156_length(amino acids)=121AA_BP=1 MTLTFLGGIKLRNDTGSQESGLFGWILNHNQEIVFIWCNHNFIFLGANSKEGEIVLGVDVTHGAPCLQDEAVHEAGILGGGGVVHGALDG -------------------------------------------------------------- |
Top |
Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:45331306/chr20:33122431) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
 |
| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| ITGB3 | DYNLRB1 |
| FUNCTION: Transcription coregulator that can have both coactivator and corepressor functions. Isoform 1, but not other isoforms, is involved in the coactivation of nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent fashion. In contrast, it does not coactivate nuclear receptors for retinoic acid, vitamin D, progesterone receptor, nor glucocorticoid. Acts as a coactivator for estrogen receptor alpha. Acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. May also act as an inhibitor of cyclin A-associated kinase. Also acts a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. {ECO:0000269|PubMed:11713274, ECO:0000269|PubMed:12244126, ECO:0000269|PubMed:15082778, ECO:0000269|PubMed:15254226, ECO:0000269|PubMed:16622420}. | FUNCTION: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 135_377 | 26.333333333333332 | 789.0 | Domain | Note=VWFA |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 777_783 | 26.333333333333332 | 789.0 | Motif | LIR |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 293_313 | 26.333333333333332 | 789.0 | Region | CX3CL1-binding |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 463_629 | 26.333333333333332 | 789.0 | Region | Note=Cysteine-rich tandem repeats |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 463_511 | 26.333333333333332 | 789.0 | Repeat | Note=I |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 512_553 | 26.333333333333332 | 789.0 | Repeat | Note=II |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 554_592 | 26.333333333333332 | 789.0 | Repeat | Note=III |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 593_629 | 26.333333333333332 | 789.0 | Repeat | Note=IV |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 27_718 | 26.333333333333332 | 789.0 | Topological domain | Extracellular |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 742_788 | 26.333333333333332 | 789.0 | Topological domain | Cytoplasmic |
| Hgene | ITGB3 | chr17:45331306 | chr20:33122431 | ENST00000559488 | + | 1 | 15 | 719_741 | 26.333333333333332 | 789.0 | Transmembrane | Helical |
Top |
Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| ITGB3 | |
| DYNLRB1 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
Top |
Related Drugs to ITGB3-DYNLRB1 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
Top |
Related Diseases to ITGB3-DYNLRB1 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
Copyright 2021-Present -The University of Texas Health Science Center at Houston (UTHealth)
Web File Viewing | Emergency Information |Campus Carry|Site Policies