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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:MIER2-CDK5RAP1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MIER2-CDK5RAP1
	FusionPDB ID: 53635
	FusionGDB2.0 ID: 53635
		Hgene	Tgene
	Gene symbol	MIER2	CDK5RAP1
	Gene ID	54531	51654
	Gene name	MIER family member 2	CDK5 regulatory subunit associated protein 1
	Synonyms	KIAA1193\|Mi-er2	C20orf34\|C42\|CGI-05\|HSPC167
	Cytomap	19p13.3	20q11.21
	Type of gene	protein-coding	protein-coding
	Description	mesoderm induction early response protein 2mesoderm induction early response 1, family member 2	CDK5 regulatory subunit-associated protein 1CDK5 activator-binding protein C42
	Modification date	20200313	20200313
	UniProtAcc	Q8N344	Q96SZ6
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000264819, ENST00000592722,	ENST00000477105, ENST00000339269, ENST00000346416, ENST00000357886, ENST00000473997, ENST00000544843,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	5 X 7 X 7=245	8 X 6 X 7=336
	# samples	7	9
	** MAII score	log2(7/245*10)=-1.8073549220576 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/336*10)=-1.90046432644909 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: MIER2 [Title/Abstract] AND CDK5RAP1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MIER2(325635)-CDK5RAP1(31975339), # samples:5
Anticipated loss of major functional domain due to fusion event.	MIER2-CDK5RAP1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MIER2-CDK5RAP1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MIER2-CDK5RAP1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MIER2-CDK5RAP1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	MIER2	GO:0016575	histone deacetylation	28046085
Tgene	CDK5RAP1	GO:0045736	negative regulation of cyclin-dependent protein serine/threonine kinase activity	11882646

Fusion gene breakpoints across MIER2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across CDK5RAP1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BLCA	TCGA-BL-A0C8-01A	MIER2	chr19	325635	-	CDK5RAP1	chr20	31975339	-
ChimerDB4	BLCA	TCGA-BL-A0C8-01B	MIER2	chr19	325635	-	CDK5RAP1	chr20	31975339	-
ChimerDB4	BLCA	TCGA-BL-A0C8	MIER2	chr19	325634	-	CDK5RAP1	chr20	31975339	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000264819	MIER2	chr19	325635	-	ENST00000473997	CDK5RAP1	chr20	31975339	-	2090	666	11	1885	624
ENST00000264819	MIER2	chr19	325635	-	ENST00000346416	CDK5RAP1	chr20	31975339	-	2090	666	11	1885	624
ENST00000264819	MIER2	chr19	325635	-	ENST00000339269	CDK5RAP1	chr20	31975339	-	1859	666	11	1654	547
ENST00000264819	MIER2	chr19	325635	-	ENST00000357886	CDK5RAP1	chr20	31975339	-	2132	666	11	1927	638
ENST00000264819	MIER2	chr19	325635	-	ENST00000544843	CDK5RAP1	chr20	31975339	-	1801	666	11	1402	463
ENST00000264819	MIER2	chr19	325634	-	ENST00000473997	CDK5RAP1	chr20	31975339	-	2090	666	11	1885	624
ENST00000264819	MIER2	chr19	325634	-	ENST00000346416	CDK5RAP1	chr20	31975339	-	2090	666	11	1885	624
ENST00000264819	MIER2	chr19	325634	-	ENST00000339269	CDK5RAP1	chr20	31975339	-	1859	666	11	1654	547
ENST00000264819	MIER2	chr19	325634	-	ENST00000357886	CDK5RAP1	chr20	31975339	-	2132	666	11	1927	638
ENST00000264819	MIER2	chr19	325634	-	ENST00000544843	CDK5RAP1	chr20	31975339	-	1801	666	11	1402	463

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000264819	ENST00000473997	MIER2	chr19	325635	-	CDK5RAP1	chr20	31975339	-	0.005159637	0.9948403
ENST00000264819	ENST00000346416	MIER2	chr19	325635	-	CDK5RAP1	chr20	31975339	-	0.005159637	0.9948403
ENST00000264819	ENST00000339269	MIER2	chr19	325635	-	CDK5RAP1	chr20	31975339	-	0.005871391	0.9941286
ENST00000264819	ENST00000357886	MIER2	chr19	325635	-	CDK5RAP1	chr20	31975339	-	0.00481453	0.99518543
ENST00000264819	ENST00000544843	MIER2	chr19	325635	-	CDK5RAP1	chr20	31975339	-	0.003178014	0.996822
ENST00000264819	ENST00000473997	MIER2	chr19	325634	-	CDK5RAP1	chr20	31975339	-	0.005159637	0.9948403
ENST00000264819	ENST00000346416	MIER2	chr19	325634	-	CDK5RAP1	chr20	31975339	-	0.005159637	0.9948403
ENST00000264819	ENST00000339269	MIER2	chr19	325634	-	CDK5RAP1	chr20	31975339	-	0.005871391	0.9941286
ENST00000264819	ENST00000357886	MIER2	chr19	325634	-	CDK5RAP1	chr20	31975339	-	0.00481453	0.99518543
ENST00000264819	ENST00000544843	MIER2	chr19	325634	-	CDK5RAP1	chr20	31975339	-	0.003178014	0.996822

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>53635_53635_1_MIER2-CDK5RAP1_MIER2_chr19_325634_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000339269_length(amino acids)=547AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQVLQLIHERDNICKQIHLPAQSGSSRVLEAMR
RGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELIT
IFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQPGDYVLVKITSASSQTLRGHVLCRTTL

--------------------------------------------------------------

>53635_53635_2_MIER2-CDK5RAP1_MIER2_chr19_325634_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000346416_length(amino acids)=624AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAVPTNL
SRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVH
HIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTS

--------------------------------------------------------------

>53635_53635_3_MIER2-CDK5RAP1_MIER2_chr19_325634_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000357886_length(amino acids)=638AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQVFLPPRPPKVLGLQGLKEVTLLGQNVNSFRD
NSEVQFNSAVPTNLSRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAM
RRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELI
TIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQPGDYVLVKITSASSQTLRGHVLCRTT

--------------------------------------------------------------

>53635_53635_4_MIER2-CDK5RAP1_MIER2_chr19_325634_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000473997_length(amino acids)=624AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAVPTNL
SRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVH
HIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTS

--------------------------------------------------------------

>53635_53635_5_MIER2-CDK5RAP1_MIER2_chr19_325634_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000544843_length(amino acids)=463AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAVPTNL
SRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVH

--------------------------------------------------------------

>53635_53635_6_MIER2-CDK5RAP1_MIER2_chr19_325635_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000339269_length(amino acids)=547AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQVLQLIHERDNICKQIHLPAQSGSSRVLEAMR
RGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELIT
IFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQPGDYVLVKITSASSQTLRGHVLCRTTL

--------------------------------------------------------------

>53635_53635_7_MIER2-CDK5RAP1_MIER2_chr19_325635_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000346416_length(amino acids)=624AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAVPTNL
SRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVH
HIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTS

--------------------------------------------------------------

>53635_53635_8_MIER2-CDK5RAP1_MIER2_chr19_325635_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000357886_length(amino acids)=638AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQVFLPPRPPKVLGLQGLKEVTLLGQNVNSFRD
NSEVQFNSAVPTNLSRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAM
RRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELI
TIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQPGDYVLVKITSASSQTLRGHVLCRTT

--------------------------------------------------------------

>53635_53635_9_MIER2-CDK5RAP1_MIER2_chr19_325635_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000473997_length(amino acids)=624AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAVPTNL
SRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVH
HIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTS

--------------------------------------------------------------

>53635_53635_10_MIER2-CDK5RAP1_MIER2_chr19_325635_ENST00000264819_CDK5RAP1_chr20_31975339_ENST00000544843_length(amino acids)=463AA_BP=219
MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL
LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS
SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE
TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAVPTNL
SRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVH

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:325635/chr20:31975339)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
MIER2 Q8N344	CDK5RAP1 Q96SZ6
FUNCTION: Transcriptional repressor. {ECO:0000250}.	FUNCTION: Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i(6)A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp) (PubMed:22422838, PubMed:25738458, PubMed:28981754). Essential for efficient and highly accurate protein translation by the ribosome (PubMed:22422838, PubMed:25738458, PubMed:28981754). Specifically inhibits CDK5 activation by CDK5R1 (PubMed:11882646). Essential for efficient mitochondrial protein synthesis and respiratory chain; shows pathological consequences in mitochondrial disease (PubMed:25738458). {ECO:0000269\|PubMed:11882646, ECO:0000269\|PubMed:22422838, ECO:0000269\|PubMed:25738458, ECO:0000269\|PubMed:28981754}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000339269

244_512

181.33333333333334

511.0

Domain

Radical SAM core

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000339269

515_590

181.33333333333334

511.0

Domain

TRAM

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000346416

244_512

181.33333333333334

588.0

Domain

Radical SAM core

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000346416

515_590

181.33333333333334

588.0

Domain

TRAM

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000357886

244_512

181.33333333333334

602.0

Domain

Radical SAM core

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000357886

515_590

181.33333333333334

602.0

Domain

TRAM

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000544843

244_512

181.33333333333334

427.6666666666667

Domain

Radical SAM core

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000544843

515_590

181.33333333333334

427.6666666666667

Domain

TRAM

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000339269

244_512

181.33333333333334

511.0

Domain

Radical SAM core

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000339269

515_590

181.33333333333334

511.0

Domain

TRAM

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000346416

244_512

181.33333333333334

588.0

Domain

Radical SAM core

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000346416

515_590

181.33333333333334

588.0

Domain

TRAM

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000357886

244_512

181.33333333333334

602.0

Domain

Radical SAM core

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000357886

515_590

181.33333333333334

602.0

Domain

TRAM

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000544843

244_512

181.33333333333334

427.6666666666667

Domain

Radical SAM core

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000544843

515_590

181.33333333333334

427.6666666666667

Domain

TRAM

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MIER2

chr19:325634

chr20:31975339

ENST00000264819

195_292

218.33333333333334

546.0

Domain

ELM2

Hgene

MIER2

chr19:325634

chr20:31975339

ENST00000264819

297_349

218.33333333333334

546.0

Domain

SANT

Hgene

MIER2

chr19:325635

chr20:31975339

ENST00000264819

195_292

218.33333333333334

546.0

Domain

ELM2

Hgene

MIER2

chr19:325635

chr20:31975339

ENST00000264819

297_349

218.33333333333334

546.0

Domain

SANT

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000339269

100_220

181.33333333333334

511.0

Domain

MTTase N-terminal

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000346416

100_220

181.33333333333334

588.0

Domain

MTTase N-terminal

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000357886

100_220

181.33333333333334

602.0

Domain

MTTase N-terminal

Tgene

CDK5RAP1

chr19:325634

chr20:31975339

ENST00000544843

100_220

181.33333333333334

427.6666666666667

Domain

MTTase N-terminal

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000339269

100_220

181.33333333333334

511.0

Domain

MTTase N-terminal

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000346416

100_220

181.33333333333334

588.0

Domain

MTTase N-terminal

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000357886

100_220

181.33333333333334

602.0

Domain

MTTase N-terminal

Tgene

CDK5RAP1

chr19:325635

chr20:31975339

ENST00000544843

100_220

181.33333333333334

427.6666666666667

Domain

MTTase N-terminal

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1338_MIER2_325635_CDK5RAP1_31975339_1338_MIER2_325635_CDK5RAP1_31975339_ranked_0.pdb	MIER2	325634	325635	ENST00000544843	CDK5RAP1	chr20	31975339	-	MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDEL LALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSAS SDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKSCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDE TYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQVFLPPRPPKVLGLQGLKEVTLLGQNVNSFRD NSEVQFNSAVPTNLSRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQLIHERDNICKQIHLPAQSGSSRVLEAM RRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHVQTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELI TIFREEATKANQTSVGCTQLVLVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQPGDYVLVKITSASSQTLRGHVLCRTT	638

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

MIER2_pLDDT.png

CDK5RAP1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
MIER2
CDK5RAP1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to MIER2-CDK5RAP1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to MIER2-CDK5RAP1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source