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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:NFE2L1-CARM1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NFE2L1-CARM1
	FusionPDB ID: 58814
	FusionGDB2.0 ID: 58814
		Hgene	Tgene
	Gene symbol	NFE2L1	CARM1
	Gene ID	4779	10498
	Gene name	nuclear factor, erythroid 2 like 1	coactivator associated arginine methyltransferase 1
	Synonyms	LCR-F1\|NRF1\|TCF11	PRMT4
	Cytomap	17q21.32	19p13.2
	Type of gene	protein-coding	protein-coding
	Description	endoplasmic reticulum membrane sensor NFE2L1NF-E2-related factor 1NFE2-related factor 1TCF-11locus control region-factor 1nuclear factor erythroid 2-related factor 1nuclear factor, erythroid derived 2, like 1protein NRF1, p120 formtranscription fa	histone-arginine methyltransferase CARM1protein arginine N-methyltransferase 4
	Modification date	20200313	20200313
	UniProtAcc	Q14494	Q86X55
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000357480, ENST00000361665, ENST00000362042, ENST00000536222, ENST00000582155, ENST00000583378, ENST00000585291, ENST00000579481,	ENST00000327064, ENST00000344150,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	18 X 12 X 13=2808	4 X 7 X 10=280
	# samples	20	12
	** MAII score	log2(20/2808*10)=-3.81147103052984 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(12/280*10)=-1.22239242133645 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: NFE2L1 [Title/Abstract] AND CARM1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NFE2L1(46134864)-CARM1(11022860), # samples:2
Anticipated loss of major functional domain due to fusion event.	NFE2L1-CARM1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NFE2L1-CARM1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NFE2L1-CARM1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NFE2L1-CARM1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	CARM1	GO:0016571	histone methylation	19405910

Fusion gene breakpoints across NFE2L1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across CARM1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-HC-7232-01A	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+
ChimerDB4	PRAD	TCGA-HC-7232	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000362042	NFE2L1	chr17	46134864	+	ENST00000327064	CARM1	chr19	11022860	+	3872	1588	616	2856	746
ENST00000362042	NFE2L1	chr17	46134864	+	ENST00000344150	CARM1	chr19	11022860	+	3752	1588	616	2787	723
ENST00000585291	NFE2L1	chr17	46134864	+	ENST00000327064	CARM1	chr19	11022860	+	3481	1197	291	2465	724
ENST00000585291	NFE2L1	chr17	46134864	+	ENST00000344150	CARM1	chr19	11022860	+	3361	1197	291	2396	701
ENST00000357480	NFE2L1	chr17	46134864	+	ENST00000327064	CARM1	chr19	11022860	+	3771	1487	605	2755	716
ENST00000357480	NFE2L1	chr17	46134864	+	ENST00000344150	CARM1	chr19	11022860	+	3651	1487	605	2686	693
ENST00000361665	NFE2L1	chr17	46134864	+	ENST00000327064	CARM1	chr19	11022860	+	3827	1543	604	2811	735
ENST00000361665	NFE2L1	chr17	46134864	+	ENST00000344150	CARM1	chr19	11022860	+	3707	1543	604	2742	712
ENST00000582155	NFE2L1	chr17	46134864	+	ENST00000327064	CARM1	chr19	11022860	+	2743	459	51	1727	558
ENST00000582155	NFE2L1	chr17	46134864	+	ENST00000344150	CARM1	chr19	11022860	+	2623	459	51	1658	535
ENST00000583378	NFE2L1	chr17	46134864	+	ENST00000327064	CARM1	chr19	11022860	+	2693	409	34	1677	547
ENST00000583378	NFE2L1	chr17	46134864	+	ENST00000344150	CARM1	chr19	11022860	+	2573	409	34	1608	524
ENST00000536222	NFE2L1	chr17	46134864	+	ENST00000327064	CARM1	chr19	11022860	+	3097	813	261	2081	606
ENST00000536222	NFE2L1	chr17	46134864	+	ENST00000344150	CARM1	chr19	11022860	+	2977	813	261	2012	583

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000362042	ENST00000327064	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.001168036	0.9988319
ENST00000362042	ENST00000344150	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.001419519	0.99858046
ENST00000585291	ENST00000327064	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.001800093	0.9981998
ENST00000585291	ENST00000344150	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.002192573	0.99780744
ENST00000357480	ENST00000327064	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.001166988	0.998833
ENST00000357480	ENST00000344150	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.001426954	0.99857306
ENST00000361665	ENST00000327064	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.001401241	0.99859875
ENST00000361665	ENST00000344150	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.001584002	0.99841595
ENST00000582155	ENST00000327064	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.002905432	0.9970945
ENST00000582155	ENST00000344150	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.002426823	0.9975732
ENST00000583378	ENST00000327064	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.004324501	0.9956755
ENST00000583378	ENST00000344150	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.003678824	0.9963212
ENST00000536222	ENST00000327064	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.007771059	0.9922289
ENST00000536222	ENST00000344150	NFE2L1	chr17	46134864	+	CARM1	chr19	11022860	+	0.00791772	0.99208224

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>58814_58814_1_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000357480_CARM1_chr19_11022860_ENST00000327064_length(amino acids)=716AA_BP=293
MLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARRLLSQVRAL
DRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDIDLIDILWR
QDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEPPALQNNLLSPL
LTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVD
IIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDT
FDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGD
TLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSS

--------------------------------------------------------------

>58814_58814_2_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000357480_CARM1_chr19_11022860_ENST00000344150_length(amino acids)=693AA_BP=293
MLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARRLLSQVRAL
DRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDIDLIDILWR
QDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEPPALQNNLLSPL
LTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVD
IIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDT
FDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGD
TLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSS

--------------------------------------------------------------

>58814_58814_3_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000361665_CARM1_chr19_11022860_ENST00000327064_length(amino acids)=735AA_BP=312
MLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARRLLSQVRAL
DRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDIDLGAGREV
FDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQVPSGEDQTALSLEECLRLLEATCPFGENAEFPADISSITE
AVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTD
RIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDL
SALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLT
HWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTY
NLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISM

--------------------------------------------------------------

>58814_58814_4_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000361665_CARM1_chr19_11022860_ENST00000344150_length(amino acids)=712AA_BP=312
MLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARRLLSQVRAL
DRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDIDLGAGREV
FDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQVPSGEDQTALSLEECLRLLEATCPFGENAEFPADISSITE
AVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTD
RIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDL
SALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLT
HWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTY

--------------------------------------------------------------

>58814_58814_5_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000362042_CARM1_chr19_11022860_ENST00000327064_length(amino acids)=746AA_BP=323
MLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARRLLSQVRAL
DRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDIDLIDILWR
QDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQVPSGEDQTALSLEECLRLLEATCPFGENA
EFPADISSITEAVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHA
EVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANF
WYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTV
WLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSP
SENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNS

--------------------------------------------------------------

>58814_58814_6_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000362042_CARM1_chr19_11022860_ENST00000344150_length(amino acids)=723AA_BP=323
MLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARRLLSQVRAL
DRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDIDLIDILWR
QDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQVPSGEDQTALSLEECLRLLEATCPFGENA
EFPADISSITEAVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHA
EVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANF
WYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTV
WLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSP
SENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPTNTMH

--------------------------------------------------------------

>58814_58814_7_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000536222_CARM1_chr19_11022860_ENST00000327064_length(amino acids)=606AA_BP=183
MSGFPAPAALGLISQTMTRLDRSSHSGGGHAKGSRPLNSLLAGHRVAQPASAPGSRASVQDIDLIDILWRQDIDLGAGREVFDYSHRQKE
QDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSI
MEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESY
LHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEA
KEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISI
VAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTG

--------------------------------------------------------------

>58814_58814_8_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000536222_CARM1_chr19_11022860_ENST00000344150_length(amino acids)=583AA_BP=183
MSGFPAPAALGLISQTMTRLDRSSHSGGGHAKGSRPLNSLLAGHRVAQPASAPGSRASVQDIDLIDILWRQDIDLGAGREVFDYSHRQKE
QDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSI
MEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESY
LHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEA
KEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISI
VAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLGSSG

--------------------------------------------------------------

>58814_58814_9_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000582155_CARM1_chr19_11022860_ENST00000327064_length(amino acids)=558AA_BP=135
MGWESHLTAASADIDLIDILWRQDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISS
ITEAVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNN
LTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHG
VDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTE
PLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTG
STYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPA

--------------------------------------------------------------

>58814_58814_10_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000582155_CARM1_chr19_11022860_ENST00000344150_length(amino acids)=535AA_BP=135
MGWESHLTAASADIDLIDILWRQDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISS
ITEAVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNN
LTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHG
VDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTE
PLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTG

--------------------------------------------------------------

>58814_58814_11_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000583378_CARM1_chr19_11022860_ENST00000327064_length(amino acids)=547AA_BP=124
MGWESHLTAASADIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEP
PALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGK
VEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAV
DEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCL
FQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAV
AGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPT

--------------------------------------------------------------

>58814_58814_12_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000583378_CARM1_chr19_11022860_ENST00000344150_length(amino acids)=524AA_BP=124
MGWESHLTAASADIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEP
PALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGK
VEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAV
DEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCL
FQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAV

--------------------------------------------------------------

>58814_58814_13_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000585291_CARM1_chr19_11022860_ENST00000327064_length(amino acids)=724AA_BP=301
MYVDCGETMLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARR
LLSQVRALDRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDI
DLIDILWRQDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEPPAL
QNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEE
VSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEY
FRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQS
PLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGM
PTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPTNTM

--------------------------------------------------------------

>58814_58814_14_NFE2L1-CARM1_NFE2L1_chr17_46134864_ENST00000585291_CARM1_chr19_11022860_ENST00000344150_length(amino acids)=701AA_BP=301
MYVDCGETMLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARR
LLSQVRALDRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDI
DLIDILWRQDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEPPAL
QNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEE
VSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEY
FRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQS
PLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGM

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:46134864/chr19:11022860)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
NFE2L1 Q14494	CARM1 Q86X55
FUNCTION: [Endoplasmic reticulum membrane sensor NFE2L1]: Endoplasmic reticulum membrane sensor that translocates into the nucleus in response to various stresses to act as a transcription factor (PubMed:20932482, PubMed:24448410). Constitutes a precursor of the transcription factor NRF1 (By similarity). Able to detect various cellular stresses, such as cholesterol excess, oxidative stress or proteasome inhibition (PubMed:20932482). In response to stress, it is released from the endoplasmic reticulum membrane following cleavage by the protease DDI2 and translocates into the nucleus to form the transcription factor NRF1 (By similarity). Acts as a key sensor of cholesterol excess: in excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal, such as CD36 (By similarity). Involved in proteasome homeostasis: in response to proteasome inhibition, it is released from the endoplasmic reticulum membrane, translocates to the nucleus and activates expression of genes encoding proteasome subunits (PubMed:20932482). {ECO:0000250\|UniProtKB:Q61985, ECO:0000269\|PubMed:20932482, ECO:0000269\|PubMed:24448410}.; FUNCTION: [Transcription factor NRF1]: CNC-type bZIP family transcription factor that translocates to the nucleus and regulates expression of target genes in response to various stresses (PubMed:8932385, PubMed:9421508). Heterodimerizes with small-Maf proteins (MAFF, MAFG or MAFK) and binds DNA motifs including the antioxidant response elements (AREs), which regulate expression of genes involved in oxidative stress response (PubMed:8932385, PubMed:9421508). Activates or represses expression of target genes, depending on the context (PubMed:8932385, PubMed:9421508). Plays a key role in cholesterol homeostasis by acting as a sensor of cholesterol excess: in low cholesterol conditions, translocates into the nucleus and represses expression of genes involved in defense against cholesterol excess, such as CD36 (By similarity). In excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal (By similarity). Critical for redox balance in response to oxidative stress: acts by binding the AREs motifs on promoters and mediating activation of oxidative stress response genes, such as GCLC, GCLM, GSS, MT1 and MT2 (By similarity). Plays an essential role during fetal liver hematopoiesis: probably has a protective function against oxidative stress and is involved in lipid homeostasis in the liver (By similarity). Involved in proteasome homeostasis: in response to proteasome inhibition, mediates the 'bounce-back' of proteasome subunits by translocating into the nucleus and activating expression of genes encoding proteasome subunits (PubMed:20932482). Also involved in regulating glucose flux (By similarity). Together with CEBPB; represses expression of DSPP during odontoblast differentiation (PubMed:15308669). In response to ascorbic acid induction, activates expression of SP7/Osterix in osteoblasts. {ECO:0000250\|UniProtKB:Q61985, ECO:0000269\|PubMed:15308669, ECO:0000269\|PubMed:20932482, ECO:0000269\|PubMed:8932385, ECO:0000269\|PubMed:9421508}.	FUNCTION: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. {ECO:0000269\|PubMed:16497732, ECO:0000269\|PubMed:19405910}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

125_288

294.0

743.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

125_288

324.0

773.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

125_288

294.0

743.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

191_199

294.0

743.0

Region

Cholesterol recognition/amino acid consensus (CRAC) region

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

191_199

324.0

773.0

Region

Cholesterol recognition/amino acid consensus (CRAC) region

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

191_199

294.0

743.0

Region

Cholesterol recognition/amino acid consensus (CRAC) region

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

7_24

294.0

743.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

7_24

324.0

773.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

7_24

294.0

743.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

CARM1

chr17:46134864

chr19:11022860

ENST00000327064

499_608

186.0

609.0

Region

Transactivation domain

Tgene

CARM1

chr17:46134864

chr19:11022860

ENST00000344150

499_608

186.0

586.0

Region

Transactivation domain

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

496_517

294.0

743.0

Compositional bias

Note=Poly-Ser

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

496_517

324.0

773.0

Compositional bias

Note=Poly-Ser

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

496_517

294.0

743.0

Compositional bias

Note=Poly-Ser

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

654_717

294.0

743.0

Domain

bZIP

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

654_717

324.0

773.0

Domain

bZIP

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

654_717

294.0

743.0

Domain

bZIP

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

476_480

294.0

743.0

Motif

Destruction motif

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

476_480

324.0

773.0

Motif

Destruction motif

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

476_480

294.0

743.0

Motif

Destruction motif

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

379_383

294.0

743.0

Region

CPD

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

656_675

294.0

743.0

Region

Basic motif

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000357480

682_696

294.0

743.0

Region

Leucine-zipper

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

379_383

324.0

773.0

Region

CPD

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

656_675

324.0

773.0

Region

Basic motif

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000362042

682_696

324.0

773.0

Region

Leucine-zipper

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

379_383

294.0

743.0

Region

CPD

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

656_675

294.0

743.0

Region

Basic motif

Hgene

NFE2L1

chr17:46134864

chr19:11022860

ENST00000585291

682_696

294.0

743.0

Region

Leucine-zipper

Tgene

CARM1

chr17:46134864

chr19:11022860

ENST00000327064

146_453

186.0

609.0

Domain

SAM-dependent MTase PRMT-type

Tgene

CARM1

chr17:46134864

chr19:11022860

ENST00000344150

146_453

186.0

586.0

Domain

SAM-dependent MTase PRMT-type

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1505_NFE2L1_46134864_CARM1_11022860_ranked_0.pdb	NFE2L1	46134864	46134864	ENST00000344150	CARM1	chr19	11022860	+	MLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARRLLSQVRAL DRFQVPTTEVNAWLVHRDPEGSVSGSQPNSGLALESSSGLQDVTGPDNGVRESETEQGFGEDLEDLGAVAPPVSGDLTKEDIDLIDILWR QDIDLGAGREVFDYSHRQKEQDVEKELRDGGEQDTWAGEGAEALARNLLVDGETGESFPAQVPSGEDQTALSLEECLRLLEATCPFGENA EFPADISSITEAVPSESEPPALQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHA EVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANF WYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTV WLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSP SENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNS	746

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

NFE2L1_pLDDT.png

CARM1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
NFE2L1
CARM1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to NFE2L1-CARM1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to NFE2L1-CARM1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source