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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:NFIB-ZDHHC21

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NFIB-ZDHHC21
	FusionPDB ID: 58899
	FusionGDB2.0 ID: 58899
		Hgene	Tgene
	Gene symbol	NFIB	ZDHHC21
	Gene ID	4781	340481
	Gene name	nuclear factor I B	zinc finger DHHC-type palmitoyltransferase 21
	Synonyms	CTF\|HMGIC/NFIB\|MACID\|NF-I/B\|NF1-B\|NFI-B\|NFI-RED\|NFIB2\|NFIB3	DHHC-21\|DHHC21\|DNZ1\|HSPC097
	Cytomap	9p23-p22.3	9p22.3
	Type of gene	protein-coding	protein-coding
	Description	nuclear factor 1 B-typeCCAAT-box-binding transcription factorTGGCA-binding proteinnuclear factor 1/B	palmitoyltransferase ZDHHC21zinc finger DHHC-type containing 21zinc finger, DHHC domain containing 21
	Modification date	20200313	20200313
	UniProtAcc	O00712	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000380921, ENST00000380934, ENST00000380953, ENST00000380959, ENST00000397575, ENST00000397579, ENST00000397581, ENST00000380924, ENST00000543693,	ENST00000497966, ENST00000380916,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	16 X 16 X 8=2048	1 X 1 X 1=1
	# samples	18	1
	** MAII score	log2(18/2048*10)=-3.50814690367033 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(1/1*10)=3.32192809488736
Context (manual curation of fusion genes in FusionPDB)	PubMed: NFIB [Title/Abstract] AND ZDHHC21 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NFIB(14306988)-ZDHHC21(14662324), # samples:3
Anticipated loss of major functional domain due to fusion event.	NFIB-ZDHHC21 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NFIB-ZDHHC21 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NFIB-ZDHHC21 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NFIB-ZDHHC21 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	NFIB	GO:0045893	positive regulation of transcription, DNA-templated	30388402
Hgene	NFIB	GO:0045944	positive regulation of transcription by RNA polymerase II	9099724\|19540848
Tgene	ZDHHC21	GO:0018345	protein palmitoylation	23034182

Fusion gene breakpoints across NFIB (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ZDHHC21 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUAD	TCGA-75-6211-01A	NFIB	chr9	14306988	-	ZDHHC21	chr9	14662324	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000380934	NFIB	chr9	14306988	-	ENST00000380916	ZDHHC21	chr9	14662324	-	9432	992	352	1536	394
ENST00000380959	NFIB	chr9	14306988	-	ENST00000380916	ZDHHC21	chr9	14662324	-	9476	1036	474	1580	368
ENST00000380953	NFIB	chr9	14306988	-	ENST00000380916	ZDHHC21	chr9	14662324	-	9158	718	156	1262	368
ENST00000397575	NFIB	chr9	14306988	-	ENST00000380916	ZDHHC21	chr9	14662324	-	9560	1120	558	1664	368
ENST00000397581	NFIB	chr9	14306988	-	ENST00000380916	ZDHHC21	chr9	14662324	-	10010	1570	1008	2114	368
ENST00000397579	NFIB	chr9	14306988	-	ENST00000380916	ZDHHC21	chr9	14662324	-	10073	1633	1071	2177	368
ENST00000380921	NFIB	chr9	14306988	-	ENST00000380916	ZDHHC21	chr9	14662324	-	9448	1008	446	1552	368

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000380934	ENST00000380916	NFIB	chr9	14306988	-	ZDHHC21	chr9	14662324	-	0.000253093	0.99974686
ENST00000380959	ENST00000380916	NFIB	chr9	14306988	-	ZDHHC21	chr9	14662324	-	0.000147677	0.9998523
ENST00000380953	ENST00000380916	NFIB	chr9	14306988	-	ZDHHC21	chr9	14662324	-	0.00013744	0.99986255
ENST00000397575	ENST00000380916	NFIB	chr9	14306988	-	ZDHHC21	chr9	14662324	-	0.000149928	0.99985003
ENST00000397581	ENST00000380916	NFIB	chr9	14306988	-	ZDHHC21	chr9	14662324	-	0.000168317	0.9998317
ENST00000397579	ENST00000380916	NFIB	chr9	14306988	-	ZDHHC21	chr9	14662324	-	0.000173524	0.9998265
ENST00000380921	ENST00000380916	NFIB	chr9	14306988	-	ZDHHC21	chr9	14662324	-	0.000147848	0.9998522

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>58899_58899_1_NFIB-ZDHHC21_NFIB_chr9_14306988_ENST00000380921_ZDHHC21_chr9_14662324_ENST00000380916_length(amino acids)=368AA_BP=187
MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYRE
DFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFL
AYYVQEQEREFWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLTCYALMFSFCHYYYFLPLKKRN
LDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNCCEDISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRV

--------------------------------------------------------------

>58899_58899_2_NFIB-ZDHHC21_NFIB_chr9_14306988_ENST00000380934_ZDHHC21_chr9_14662324_ENST00000380916_length(amino acids)=394AA_BP=213
MERIPVSVDFWVVCCAVLKCNPGIPKRMSTLCFGFSDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSE
KPEIKQKWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKS
PHCTNPALCVQPHHITVSVKELDLFLAYYVQEQEREFWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFY
TELLTCYALMFSFCHYYYFLPLKKRNLDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNCCEDISRPRKPWQQ

--------------------------------------------------------------

>58899_58899_3_NFIB-ZDHHC21_NFIB_chr9_14306988_ENST00000380953_ZDHHC21_chr9_14662324_ENST00000380916_length(amino acids)=368AA_BP=187
MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYRE
DFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFL
AYYVQEQEREFWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLTCYALMFSFCHYYYFLPLKKRN
LDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNCCEDISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRV

--------------------------------------------------------------

>58899_58899_4_NFIB-ZDHHC21_NFIB_chr9_14306988_ENST00000380959_ZDHHC21_chr9_14662324_ENST00000380916_length(amino acids)=368AA_BP=187
MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYRE
DFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFL
AYYVQEQEREFWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLTCYALMFSFCHYYYFLPLKKRN
LDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNCCEDISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRV

--------------------------------------------------------------

>58899_58899_5_NFIB-ZDHHC21_NFIB_chr9_14306988_ENST00000397575_ZDHHC21_chr9_14662324_ENST00000380916_length(amino acids)=368AA_BP=187
MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYRE
DFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFL
AYYVQEQEREFWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLTCYALMFSFCHYYYFLPLKKRN
LDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNCCEDISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRV

--------------------------------------------------------------

>58899_58899_6_NFIB-ZDHHC21_NFIB_chr9_14306988_ENST00000397579_ZDHHC21_chr9_14662324_ENST00000380916_length(amino acids)=368AA_BP=187
MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYRE
DFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFL
AYYVQEQEREFWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLTCYALMFSFCHYYYFLPLKKRN
LDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNCCEDISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRV

--------------------------------------------------------------

>58899_58899_7_NFIB-ZDHHC21_NFIB_chr9_14306988_ENST00000397581_ZDHHC21_chr9_14662324_ENST00000380916_length(amino acids)=368AA_BP=187
MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYRE
DFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFL
AYYVQEQEREFWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLTCYALMFSFCHYYYFLPLKKRN
LDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNCCEDISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRV

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr9:14306988/chr9:14662324)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
NFIB O00712	.
FUNCTION: Transcriptional activator of GFAP, essential for proper brain development (PubMed:30388402). Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. {ECO:0000269\|PubMed:30388402}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

90_140

84.33333333333333

266.0

Domain

DHHC

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

155_185

84.33333333333333

266.0

Topological domain

Extracellular

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

207_265

84.33333333333333

266.0

Topological domain

Cytoplasmic

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

134_154

84.33333333333333

266.0

Transmembrane

Helical

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

186_206

84.33333333333333

266.0

Transmembrane

Helical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NFIB

chr9:14306988

chr9:14662324

ENST00000380953

1_195

187.33333333333334

495.0

DNA binding

CTF/NF-I

Hgene

NFIB

chr9:14306988

chr9:14662324

ENST00000380959

1_195

187.33333333333334

421.0

DNA binding

CTF/NF-I

Hgene

NFIB

chr9:14306988

chr9:14662324

ENST00000380953

397_405

187.33333333333334

495.0

Motif

9aaTAD

Hgene

NFIB

chr9:14306988

chr9:14662324

ENST00000380959

397_405

187.33333333333334

421.0

Motif

9aaTAD

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

1_16

84.33333333333333

266.0

Topological domain

Cytoplasmic

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

38_44

84.33333333333333

266.0

Topological domain

Extracellular

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

66_133

84.33333333333333

266.0

Topological domain

Cytoplasmic

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

17_37

84.33333333333333

266.0

Transmembrane

Helical

Tgene

ZDHHC21

chr9:14306988

chr9:14662324

ENST00000380916

45_65

84.33333333333333

266.0

Transmembrane

Helical

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
NFIB
ZDHHC21

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to NFIB-ZDHHC21

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to NFIB-ZDHHC21

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	NFIB	C0010606	Adenoid Cystic Carcinoma	1	CTD_human
Hgene	NFIB	C0221355	Macrocephaly	1	GENOMICS_ENGLAND
Hgene	NFIB	C0557874	Global developmental delay	1	GENOMICS_ENGLAND
Hgene	NFIB	C3714756	Intellectual Disability	1	GENOMICS_ENGLAND
Hgene	NFIB	C4748993	MACROCEPHALY, ACQUIRED, WITH IMPAIRED INTELLECTUAL DEVELOPMENT	1	GENOMICS_ENGLAND;UNIPROT