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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:NFX1-ACO1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NFX1-ACO1
	FusionPDB ID: 59003
	FusionGDB2.0 ID: 59003
		Hgene	Tgene
	Gene symbol	NFX1	ACO1
	Gene ID	4799	48
	Gene name	nuclear transcription factor, X-box binding 1	aconitase 1
	Synonyms	NFX2\|TEG-42\|Tex42	ACONS\|HEL60\|IREB1\|IREBP\|IREBP1\|IRP1
	Cytomap	9p13.3	9p21.1
	Type of gene	protein-coding	protein-coding
	Description	transcriptional repressor NF-X1nuclear transcription factor, X box-binding protein 1	cytoplasmic aconitate hydrataseaconitase 1, solubleaconitate hydratase, cytoplasmiccitrate hydro-lyasecytoplasmic aconitasecytosplasmic aconitaseepididymis luminal protein 60epididymis secretory sperm binding proteinferritin repressor proteiniron
	Modification date	20200313	20200313
	UniProtAcc	ZNFX1	P21399
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000379521, ENST00000379540, ENST00000318524, ENST00000463421,	ENST00000309951, ENST00000379923, ENST00000541043,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	16 X 12 X 11=2112	9 X 12 X 5=540
	# samples	22	12
	** MAII score	log2(22/2112*10)=-3.26303440583379 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(12/540*10)=-2.16992500144231 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: NFX1 [Title/Abstract] AND ACO1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NFX1(33351788)-ACO1(32440463), # samples:3
Anticipated loss of major functional domain due to fusion event.	NFX1-ACO1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NFX1-ACO1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NFX1-ACO1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NFX1-ACO1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	NFX1	GO:0000122	negative regulation of transcription by RNA polymerase II	7964459
Hgene	NFX1	GO:0051865	protein autoubiquitination	10500182
Tgene	ACO1	GO:0006101	citrate metabolic process	8041788\|16527810
Tgene	ACO1	GO:0010040	response to iron(II) ion	8041788

Fusion gene breakpoints across NFX1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ACO1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUSC	TCGA-18-4721-01A	NFX1	chr9	33351788	-	ACO1	chr9	32440463	+
ChimerDB4	LUSC	TCGA-18-4721-01A	NFX1	chr9	33351788	+	ACO1	chr9	32440463	+
ChimerDB4	LUSC	TCGA-18-4721	NFX1	chr9	33351788	+	ACO1	chr9	32440463	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000379540	NFX1	chr9	33351788	+	ENST00000541043	ACO1	chr9	32440463	+	3861	2717	50	3139	1029
ENST00000379540	NFX1	chr9	33351788	+	ENST00000379923	ACO1	chr9	32440463	+	3865	2717	50	3139	1029
ENST00000379540	NFX1	chr9	33351788	+	ENST00000309951	ACO1	chr9	32440463	+	7798	2717	50	3139	1029
ENST00000379521	NFX1	chr9	33351788	+	ENST00000541043	ACO1	chr9	32440463	+	3860	2716	49	3138	1029
ENST00000379521	NFX1	chr9	33351788	+	ENST00000379923	ACO1	chr9	32440463	+	3864	2716	49	3138	1029
ENST00000379521	NFX1	chr9	33351788	+	ENST00000309951	ACO1	chr9	32440463	+	7797	2716	49	3138	1029

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000379540	ENST00000541043	NFX1	chr9	33351788	+	ACO1	chr9	32440463	+	0.000316836	0.99968314
ENST00000379540	ENST00000379923	NFX1	chr9	33351788	+	ACO1	chr9	32440463	+	0.000315189	0.9996848
ENST00000379540	ENST00000309951	NFX1	chr9	33351788	+	ACO1	chr9	32440463	+	0.00015813	0.9998418
ENST00000379521	ENST00000541043	NFX1	chr9	33351788	+	ACO1	chr9	32440463	+	0.000316545	0.9996835
ENST00000379521	ENST00000379923	NFX1	chr9	33351788	+	ACO1	chr9	32440463	+	0.000314899	0.99968517
ENST00000379521	ENST00000309951	NFX1	chr9	33351788	+	ACO1	chr9	32440463	+	0.000158123	0.9998418

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>59003_59003_1_NFX1-ACO1_NFX1_chr9_33351788_ENST00000379521_ACO1_chr9_32440463_ENST00000309951_length(amino acids)=1029AA_BP=889
MRHGMAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTS
FQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVY
SYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEG
HRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKY
ECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRK
KQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGST
SRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVC
GKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICG
RKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGK
HEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKLDVFDAAERYQ
QAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQ

--------------------------------------------------------------

>59003_59003_2_NFX1-ACO1_NFX1_chr9_33351788_ENST00000379521_ACO1_chr9_32440463_ENST00000379923_length(amino acids)=1029AA_BP=889
MRHGMAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTS
FQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVY
SYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEG
HRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKY
ECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRK
KQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGST
SRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVC
GKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICG
RKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGK
HEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKLDVFDAAERYQ
QAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQ

--------------------------------------------------------------

>59003_59003_3_NFX1-ACO1_NFX1_chr9_33351788_ENST00000379521_ACO1_chr9_32440463_ENST00000541043_length(amino acids)=1029AA_BP=889
MRHGMAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTS
FQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVY
SYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEG
HRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKY
ECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRK
KQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGST
SRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVC
GKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICG
RKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGK
HEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKLDVFDAAERYQ
QAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQ

--------------------------------------------------------------

>59003_59003_4_NFX1-ACO1_NFX1_chr9_33351788_ENST00000379540_ACO1_chr9_32440463_ENST00000309951_length(amino acids)=1029AA_BP=889
MRHGMAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTS
FQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVY
SYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEG
HRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKY
ECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRK
KQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGST
SRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVC
GKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICG
RKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGK
HEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKLDVFDAAERYQ
QAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQ

--------------------------------------------------------------

>59003_59003_5_NFX1-ACO1_NFX1_chr9_33351788_ENST00000379540_ACO1_chr9_32440463_ENST00000379923_length(amino acids)=1029AA_BP=889
MRHGMAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTS
FQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVY
SYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEG
HRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKY
ECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRK
KQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGST
SRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVC
GKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICG
RKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGK
HEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKLDVFDAAERYQ
QAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQ

--------------------------------------------------------------

>59003_59003_6_NFX1-ACO1_NFX1_chr9_33351788_ENST00000379540_ACO1_chr9_32440463_ENST00000541043_length(amino acids)=1029AA_BP=889
MRHGMAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTS
FQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVY
SYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEG
HRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKY
ECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRK
KQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGST
SRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVC
GKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICG
RKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGK
HEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKLDVFDAAERYQ
QAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQ

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr9:33351788/chr9:32440463)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
NFX1 ZNFX1	ACO1 P21399
1918	FUNCTION: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. {ECO:0000269\|PubMed:1946430, ECO:0000269\|PubMed:23891004, ECO:0000269\|PubMed:8041788}.; FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. {ECO:0000250}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

358_409

885.0

1025.0

Zinc finger

RING-type%3B atypical

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

453_471

885.0

1025.0

Zinc finger

Note=NF-X1-type 1

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

506_525

885.0

1025.0

Zinc finger

Note=NF-X1-type 2

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

567_586

885.0

1025.0

Zinc finger

Note=NF-X1-type 3

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

632_655

885.0

1025.0

Zinc finger

Note=NF-X1-type 4

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

694_713

885.0

1025.0

Zinc finger

Note=NF-X1-type 5

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

721_740

885.0

1025.0

Zinc finger

Note=NF-X1-type 6

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

832_854

885.0

1025.0

Zinc finger

Note=NF-X1-type 7

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

863_884

885.0

1025.0

Zinc finger

Note=NF-X1-type 8

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

358_409

885.0

1121.0

Zinc finger

RING-type%3B atypical

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

453_471

885.0

1121.0

Zinc finger

Note=NF-X1-type 1

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

506_525

885.0

1121.0

Zinc finger

Note=NF-X1-type 2

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

567_586

885.0

1121.0

Zinc finger

Note=NF-X1-type 3

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

632_655

885.0

1121.0

Zinc finger

Note=NF-X1-type 4

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

694_713

885.0

1121.0

Zinc finger

Note=NF-X1-type 5

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

721_740

885.0

1121.0

Zinc finger

Note=NF-X1-type 6

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

832_854

885.0

1121.0

Zinc finger

Note=NF-X1-type 7

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

863_884

885.0

1121.0

Zinc finger

Note=NF-X1-type 8

Tgene

ACO1

chr9:33351788

chr9:32440463

ENST00000309951

779_780

749.0

890.0

Region

Substrate binding

Tgene

ACO1

chr9:33351788

chr9:32440463

ENST00000379923

779_780

749.0

890.0

Region

Substrate binding

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

1084_1089

834.0

Compositional bias

Note=Poly-Pro

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

1084_1089

885.0

1025.0

Compositional bias

Note=Poly-Pro

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

1084_1089

885.0

1121.0

Compositional bias

Note=Poly-Pro

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

994_1062

834.0

Domain

R3H

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379521

994_1062

885.0

1025.0

Domain

R3H

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000379540

994_1062

885.0

1121.0

Domain

R3H

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

358_409

834.0

Zinc finger

RING-type%3B atypical

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

453_471

834.0

Zinc finger

Note=NF-X1-type 1

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

506_525

834.0

Zinc finger

Note=NF-X1-type 2

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

567_586

834.0

Zinc finger

Note=NF-X1-type 3

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

632_655

834.0

Zinc finger

Note=NF-X1-type 4

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

694_713

834.0

Zinc finger

Note=NF-X1-type 5

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

721_740

834.0

Zinc finger

Note=NF-X1-type 6

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

832_854

834.0

Zinc finger

Note=NF-X1-type 7

Hgene

NFX1

chr9:33351788

chr9:32440463

ENST00000318524

863_884

834.0

Zinc finger

Note=NF-X1-type 8

Tgene

ACO1

chr9:33351788

chr9:32440463

ENST00000309951

205_207

749.0

890.0

Region

Substrate binding

Tgene

ACO1

chr9:33351788

chr9:32440463

ENST00000379923

205_207

749.0

890.0

Region

Substrate binding

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1813_NFX1_33351788_ACO1_32440463_ranked_0.pdb	NFX1	33351788	33351788	ENST00000309951	ACO1	chr9	32440463	+	MRHGMAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTS FQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVY SYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEG HRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKY ECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRK KQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGST SRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVC GKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICG RKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGK HEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKLDVFDAAERYQ QAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQ	1029

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

NFX1_pLDDT.png

ACO1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
NFX1
ACO1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	NFX1	chr9:33351788	chr9:32440463	ENST00000318524	+	1	16	9_26	0	834.0	PABPC1 and PABC4

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Related Drugs to NFX1-ACO1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to NFX1-ACO1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source