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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:RPL10-GNB2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: RPL10-GNB2
	FusionPDB ID: 75768
	FusionGDB2.0 ID: 75768
		Hgene	Tgene
	Gene symbol	RPL10	GNB2
	Gene ID	6138	2783
	Gene name	ribosomal protein L15	G protein subunit beta 2
	Synonyms	DBA12\|EC45\|L15\|RPL10\|RPLY10\|RPYL10	-
	Cytomap	3p24.2	7q22.1
	Type of gene	protein-coding	protein-coding
	Description	60S ribosomal protein L15large ribosomal subunit protein eL15	guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2G protein, beta-2 subunitepididymis secretory sperm binding proteing protein subunit beta-2guanine nucleotide binding protein (G protein), beta polypeptide 2guanine nucleotide-binding pr
	Modification date	20200313	20200313
	UniProtAcc	.	P62879
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000479366, ENST00000369817, ENST00000424325, ENST00000406022,	ENST00000303210, ENST00000424361, ENST00000436220, ENST00000419828, ENST00000393924, ENST00000393926, ENST00000427895,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	11 X 12 X 7=924	6 X 7 X 2=84
	# samples	17	7
	** MAII score	log2(17/924*10)=-2.44235810527836 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(7/84*10)=-0.263034405833794 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: RPL10 [Title/Abstract] AND GNB2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	RPL10(153626883)-GNB2(100274974), # samples:1
Anticipated loss of major functional domain due to fusion event.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across RPL10 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across GNB2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	STAD	TCGA-CD-8531	RPL10	chrX	153626883	+	GNB2	chr7	100274974	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000369817	RPL10	chrX	153626883	+	ENST00000303210	GNB2	chr7	100274974	+	1792	599	441	1418	325
ENST00000369817	RPL10	chrX	153626883	+	ENST00000436220	GNB2	chr7	100274974	+	1766	599	441	1418	325
ENST00000369817	RPL10	chrX	153626883	+	ENST00000424361	GNB2	chr7	100274974	+	1756	599	441	1418	325
ENST00000424325	RPL10	chrX	153626883	+	ENST00000303210	GNB2	chr7	100274974	+	1404	211	53	1030	325
ENST00000424325	RPL10	chrX	153626883	+	ENST00000436220	GNB2	chr7	100274974	+	1378	211	53	1030	325
ENST00000424325	RPL10	chrX	153626883	+	ENST00000424361	GNB2	chr7	100274974	+	1368	211	53	1030	325

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000369817	ENST00000303210	RPL10	chrX	153626883	+	GNB2	chr7	100274974	+	0.004872002	0.99512804
ENST00000369817	ENST00000436220	RPL10	chrX	153626883	+	GNB2	chr7	100274974	+	0.004718884	0.99528104
ENST00000369817	ENST00000424361	RPL10	chrX	153626883	+	GNB2	chr7	100274974	+	0.004854643	0.9951454
ENST00000424325	ENST00000303210	RPL10	chrX	153626883	+	GNB2	chr7	100274974	+	0.005770349	0.9942297
ENST00000424325	ENST00000436220	RPL10	chrX	153626883	+	GNB2	chr7	100274974	+	0.005637464	0.9943625
ENST00000424325	ENST00000424361	RPL10	chrX	153626883	+	GNB2	chr7	100274974	+	0.005959948	0.99404013

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>75768_75768_1_RPL10-GNB2_RPL10_chrX_153626883_ENST00000369817_GNB2_chr7_100274974_ENST00000303210_length(amino acids)=325AA_BP=53
MTERPPVFDRTLWFLRAQTDRLYKPCAGGGAPLSLRCATEDPGVAMGRRPARWLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAY
APSGNFVACGGLDNICSIYSLKTREGNVRVSRELPGHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPD
GRTFVSGACDASIKLWDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSRSGRLL

--------------------------------------------------------------

>75768_75768_2_RPL10-GNB2_RPL10_chrX_153626883_ENST00000369817_GNB2_chr7_100274974_ENST00000424361_length(amino acids)=325AA_BP=53
MTERPPVFDRTLWFLRAQTDRLYKPCAGGGAPLSLRCATEDPGVAMGRRPARWLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAY
APSGNFVACGGLDNICSIYSLKTREGNVRVSRELPGHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPD
GRTFVSGACDASIKLWDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSRSGRLL

--------------------------------------------------------------

>75768_75768_3_RPL10-GNB2_RPL10_chrX_153626883_ENST00000369817_GNB2_chr7_100274974_ENST00000436220_length(amino acids)=325AA_BP=53
MTERPPVFDRTLWFLRAQTDRLYKPCAGGGAPLSLRCATEDPGVAMGRRPARWLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAY
APSGNFVACGGLDNICSIYSLKTREGNVRVSRELPGHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPD
GRTFVSGACDASIKLWDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSRSGRLL

--------------------------------------------------------------

>75768_75768_4_RPL10-GNB2_RPL10_chrX_153626883_ENST00000424325_GNB2_chr7_100274974_ENST00000303210_length(amino acids)=325AA_BP=53
MTERPPVFDRTLWFLRAQTDRLYKPCAGGGAPLSLRCATEDPGVAMGRRPARWLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAY
APSGNFVACGGLDNICSIYSLKTREGNVRVSRELPGHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPD
GRTFVSGACDASIKLWDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSRSGRLL

--------------------------------------------------------------

>75768_75768_5_RPL10-GNB2_RPL10_chrX_153626883_ENST00000424325_GNB2_chr7_100274974_ENST00000424361_length(amino acids)=325AA_BP=53
MTERPPVFDRTLWFLRAQTDRLYKPCAGGGAPLSLRCATEDPGVAMGRRPARWLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAY
APSGNFVACGGLDNICSIYSLKTREGNVRVSRELPGHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPD
GRTFVSGACDASIKLWDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSRSGRLL

--------------------------------------------------------------

>75768_75768_6_RPL10-GNB2_RPL10_chrX_153626883_ENST00000424325_GNB2_chr7_100274974_ENST00000436220_length(amino acids)=325AA_BP=53
MTERPPVFDRTLWFLRAQTDRLYKPCAGGGAPLSLRCATEDPGVAMGRRPARWLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAY
APSGNFVACGGLDNICSIYSLKTREGNVRVSRELPGHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPD
GRTFVSGACDASIKLWDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSRSGRLL

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chrX:153626883/chr7:100274974)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	GNB2 P62879
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000303210

141_170

67.66666666666667

341.0

Repeat

Note=WD 3

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000303210

182_212

67.66666666666667

341.0

Repeat

Note=WD 4

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000303210

224_254

67.66666666666667

341.0

Repeat

Note=WD 5

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000303210

268_298

67.66666666666667

341.0

Repeat

Note=WD 6

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000303210

310_340

67.66666666666667

341.0

Repeat

Note=WD 7

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000303210

95_125

67.66666666666667

341.0

Repeat

Note=WD 2

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393924

141_170

67.66666666666667

341.0

Repeat

Note=WD 3

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393924

182_212

67.66666666666667

341.0

Repeat

Note=WD 4

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393924

224_254

67.66666666666667

341.0

Repeat

Note=WD 5

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393924

268_298

67.66666666666667

341.0

Repeat

Note=WD 6

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393924

310_340

67.66666666666667

341.0

Repeat

Note=WD 7

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393924

95_125

67.66666666666667

341.0

Repeat

Note=WD 2

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393926

141_170

67.66666666666667

341.0

Repeat

Note=WD 3

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393926

182_212

67.66666666666667

341.0

Repeat

Note=WD 4

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393926

224_254

67.66666666666667

341.0

Repeat

Note=WD 5

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393926

268_298

67.66666666666667

341.0

Repeat

Note=WD 6

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393926

310_340

67.66666666666667

341.0

Repeat

Note=WD 7

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393926

95_125

67.66666666666667

341.0

Repeat

Note=WD 2

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000303210

53_83

67.66666666666667

341.0

Repeat

Note=WD 1

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393924

53_83

67.66666666666667

341.0

Repeat

Note=WD 1

Tgene

GNB2

chrX:153626883

chr7:100274974

ENST00000393926

53_83

67.66666666666667

341.0

Repeat

Note=WD 1

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
RPL10
GNB2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to RPL10-GNB2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to RPL10-GNB2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source