UTHEALTH HOME    ABOUT SBMI    A-Z    WEBMAIL    INSIDE THE UNIVERSITY
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use

Center for Computational Systems Medicine level1
leaf

Fusion Gene Summary

leaf

Fusion Gene Sample Information

leaf

Fusion ORF Analysis

leaf

Fusion Amino Acid Sequences

leaf

Fusion Protein Functional Features

leaf

Fusion Protein-Protein Interaction

leaf

Related drugs with this fusion protein

leaf

Related disease with this fusion protein

Fusion Protein:SFXN4-SIRT3

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: SFXN4-SIRT3
FusionPDB ID: 81226
FusionGDB2.0 ID: 81226
HgeneTgene
Gene symbol

SFXN4

SIRT3

Gene ID

119559

23410

Gene namesideroflexin 4sirtuin 3
SynonymsBCRM1|COXPD18|SLC56A4SIR2L3
Cytomap

10q26.11

11p15.5

Type of geneprotein-codingprotein-coding
Descriptionsideroflexin-4breast cancer resistance marker 1NAD-dependent protein deacetylase sirtuin-3, mitochondrialNAD-dependent deacetylase sirtuin-3, mitochondrialSIR2-like protein 3mitochondrial nicotinamide adenine dinucleotide-dependent deacetylaseregulatory protein SIR2 homolog 3silent mating type in
Modification date2020031320200329
UniProtAcc..
Ensembl transtripts involved in fusion geneENST idsENST00000330036, ENST00000355697, 
ENST00000461438, 
ENST00000528702, 
ENST00000529382, ENST00000532956, 
ENST00000382743, ENST00000524564, 
ENST00000525319, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score1 X 1 X 1=17 X 5 X 5=175
# samples 17
** MAII scorelog2(1/1*10)=3.32192809488736log2(7/175*10)=-1.32192809488736
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: SFXN4 [Title/Abstract] AND SIRT3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)SFXN4(120925048)-SIRT3(224239), # samples:1
Anticipated loss of major functional domain due to fusion event.SFXN4-SIRT3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
SFXN4-SIRT3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
SFXN4-SIRT3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
SFXN4-SIRT3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneSIRT3

GO:0006476

protein deacetylation

16788062

TgeneSIRT3

GO:1901671

positive regulation of superoxide dismutase activity

25210848

TgeneSIRT3

GO:1902553

positive regulation of catalase activity

25210848


check buttonFusion gene breakpoints across SFXN4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across SIRT3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4SKCMTCGA-GN-A26CSFXN4chr10

120925048

-SIRT3chr11

224239

-


Top

Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000355697SFXN4chr10120925048-ENST00000382743SIRT3chr11224239-176413120523167
ENST00000355697SFXN4chr10120925048-ENST00000525319SIRT3chr11224239-101613120523167
ENST00000355697SFXN4chr10120925048-ENST00000524564SIRT3chr11224239-88513120577185
ENST00000330036SFXN4chr10120925048-ENST00000382743SIRT3chr11224239-176012716519167
ENST00000330036SFXN4chr10120925048-ENST00000525319SIRT3chr11224239-101212716519167
ENST00000330036SFXN4chr10120925048-ENST00000524564SIRT3chr11224239-88112716573185

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000355697ENST00000382743SFXN4chr10120925048-SIRT3chr11224239-0.0356078780.9643922
ENST00000355697ENST00000525319SFXN4chr10120925048-SIRT3chr11224239-0.037173220.9628268
ENST00000355697ENST00000524564SFXN4chr10120925048-SIRT3chr11224239-0.059507510.9404925
ENST00000330036ENST00000382743SFXN4chr10120925048-SIRT3chr11224239-0.0255341230.9744659
ENST00000330036ENST00000525319SFXN4chr10120925048-SIRT3chr11224239-0.0290513910.97094864
ENST00000330036ENST00000524564SFXN4chr10120925048-SIRT3chr11224239-0.051061780.9489382

Top

Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>81226_81226_1_SFXN4-SIRT3_SFXN4_chr10_120925048_ENST00000330036_SIRT3_chr11_224239_ENST00000382743_length(amino acids)=167AA_BP=36
MSLEQEEETQPGRLLGRRDAVPAFIEPNVRFWITERQADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSL

--------------------------------------------------------------

>81226_81226_2_SFXN4-SIRT3_SFXN4_chr10_120925048_ENST00000330036_SIRT3_chr11_224239_ENST00000524564_length(amino acids)=185AA_BP=36
MSLEQEEETQPGRLLGRRDAVPAFIEPNVRFWITERQADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSL
EVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKVQTAEEDHPRGCPSHCSRL

--------------------------------------------------------------

>81226_81226_3_SFXN4-SIRT3_SFXN4_chr10_120925048_ENST00000330036_SIRT3_chr11_224239_ENST00000525319_length(amino acids)=167AA_BP=36
MSLEQEEETQPGRLLGRRDAVPAFIEPNVRFWITERQADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSL

--------------------------------------------------------------

>81226_81226_4_SFXN4-SIRT3_SFXN4_chr10_120925048_ENST00000355697_SIRT3_chr11_224239_ENST00000382743_length(amino acids)=167AA_BP=36
MSLEQEEETQPGRLLGRRDAVPAFIEPNVRFWITERQADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSL

--------------------------------------------------------------

>81226_81226_5_SFXN4-SIRT3_SFXN4_chr10_120925048_ENST00000355697_SIRT3_chr11_224239_ENST00000524564_length(amino acids)=185AA_BP=36
MSLEQEEETQPGRLLGRRDAVPAFIEPNVRFWITERQADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSL
EVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKVQTAEEDHPRGCPSHCSRL

--------------------------------------------------------------

>81226_81226_6_SFXN4-SIRT3_SFXN4_chr10_120925048_ENST00000355697_SIRT3_chr11_224239_ENST00000525319_length(amino acids)=167AA_BP=36
MSLEQEEETQPGRLLGRRDAVPAFIEPNVRFWITERQADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSL

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:120925048/chr11:224239)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneSIRT3chr10:120925048chr11:224239ENST0000052938237126_382127.0258.0DomainDeacetylase sirtuin-type
TgeneSIRT3chr10:120925048chr11:224239ENST0000038274337319_321269.0400.0Nucleotide bindingNAD
TgeneSIRT3chr10:120925048chr11:224239ENST0000038274337344_346269.0400.0Nucleotide bindingNAD
TgeneSIRT3chr10:120925048chr11:224239ENST0000052938237145_165127.0258.0Nucleotide bindingNAD
TgeneSIRT3chr10:120925048chr11:224239ENST0000052938237228_231127.0258.0Nucleotide bindingNAD
TgeneSIRT3chr10:120925048chr11:224239ENST0000052938237319_321127.0258.0Nucleotide bindingNAD
TgeneSIRT3chr10:120925048chr11:224239ENST0000052938237344_346127.0258.0Nucleotide bindingNAD

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneSFXN4chr10:120925048chr11:224239ENST00000355697-114111_13137.0338.0TransmembraneHelical
HgeneSFXN4chr10:120925048chr11:224239ENST00000355697-114133_15337.0338.0TransmembraneHelical
HgeneSFXN4chr10:120925048chr11:224239ENST00000355697-114165_18537.0338.0TransmembraneHelical
HgeneSFXN4chr10:120925048chr11:224239ENST00000355697-114251_27137.0338.0TransmembraneHelical
HgeneSFXN4chr10:120925048chr11:224239ENST00000355697-114293_31337.0338.0TransmembraneHelical
TgeneSIRT3chr10:120925048chr11:224239ENST0000038274337126_382269.0400.0DomainDeacetylase sirtuin-type
TgeneSIRT3chr10:120925048chr11:224239ENST0000038274337145_165269.0400.0Nucleotide bindingNAD
TgeneSIRT3chr10:120925048chr11:224239ENST0000038274337228_231269.0400.0Nucleotide bindingNAD


Top

Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
SFXN4
SIRT3


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs to SFXN4-SIRT3


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to SFXN4-SIRT3


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource