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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:SPAST-MEMO1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: SPAST-MEMO1
	FusionPDB ID: 85531
	FusionGDB2.0 ID: 85531
		Hgene	Tgene
	Gene symbol	SPAST	MEMO1
	Gene ID	6683	51072
	Gene name	spastin	mediator of cell motility 1
	Synonyms	ADPSP\|FSP2\|SPG4	C2orf4\|CGI-27\|MEMO\|NS5ATP7
	Cytomap	2p22.3	2p22.3
	Type of gene	protein-coding	protein-coding
	Description	spastinspastic paraplegia 4 (autosomal dominant; spastin)spastic paraplegia 4 protein	protein MEMO1C21orf19-like proteinHCV NS5A-transactivated protein 7hepatitis C virus NS5A-transactivated protein 7mediator of ErbB2-driven cell motility 1
	Modification date	20200322	20200329
	UniProtAcc	SPAST	Q9Y316
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000315285, ENST00000345662,	ENST00000407893, ENST00000426310, ENST00000490459, ENST00000295065, ENST00000379383, ENST00000404530,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 4 X 7=168	3 X 3 X 3=27
	# samples	7	4
	** MAII score	log2(7/168*10)=-1.26303440583379 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(4/27*10)=0.567040592723894 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: SPAST [Title/Abstract] AND MEMO1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	SPAST(32289315)-MEMO1(32168452), # samples:3
Anticipated loss of major functional domain due to fusion event.	SPAST-MEMO1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SPAST-MEMO1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SPAST-MEMO1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SPAST-MEMO1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	SPAST	GO:0001578	microtubule bundle formation	16219033
Hgene	SPAST	GO:0034214	protein hexamerization	17389232
Hgene	SPAST	GO:0051013	microtubule severing	15716377\|16219033\|17389232\|23745751\|26875866
Hgene	SPAST	GO:0051260	protein homooligomerization	17389232

Fusion gene breakpoints across SPAST (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across MEMO1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-HC-7737	SPAST	chr2	32314674	+	MEMO1	chr2	32168452	-
ChimerDB4	STAD	TCGA-HU-A4GF-01A	SPAST	chr2	32289315	+	MEMO1	chr2	32168452	-
ChimerDB4	STAD	TCGA-HU-A4GF	SPAST	chr2	32289315	+	MEMO1	chr2	32168452	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000345662	SPAST	chr2	32289315	+	ENST00000295065	MEMO1	chr2	32168452	-	4770	636	221	1468	415
ENST00000345662	SPAST	chr2	32289315	+	ENST00000379383	MEMO1	chr2	32168452	-	2007	636	221	1468	415
ENST00000345662	SPAST	chr2	32289315	+	ENST00000404530	MEMO1	chr2	32168452	-	2007	636	221	1468	415
ENST00000315285	SPAST	chr2	32289315	+	ENST00000295065	MEMO1	chr2	32168452	-	4674	540	125	1372	415
ENST00000315285	SPAST	chr2	32289315	+	ENST00000379383	MEMO1	chr2	32168452	-	1911	540	125	1372	415
ENST00000315285	SPAST	chr2	32289315	+	ENST00000404530	MEMO1	chr2	32168452	-	1911	540	125	1372	415
ENST00000345662	SPAST	chr2	32314674	+	ENST00000295065	MEMO1	chr2	32168452	-	4941	807	221	1639	472
ENST00000345662	SPAST	chr2	32314674	+	ENST00000379383	MEMO1	chr2	32168452	-	2178	807	221	1639	472
ENST00000345662	SPAST	chr2	32314674	+	ENST00000404530	MEMO1	chr2	32168452	-	2178	807	221	1639	472
ENST00000315285	SPAST	chr2	32314674	+	ENST00000295065	MEMO1	chr2	32168452	-	4845	711	125	1543	472
ENST00000315285	SPAST	chr2	32314674	+	ENST00000379383	MEMO1	chr2	32168452	-	2082	711	125	1543	472
ENST00000315285	SPAST	chr2	32314674	+	ENST00000404530	MEMO1	chr2	32168452	-	2082	711	125	1543	472

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000345662	ENST00000295065	SPAST	chr2	32289315	+	MEMO1	chr2	32168452	-	0.000316071	0.999684
ENST00000345662	ENST00000379383	SPAST	chr2	32289315	+	MEMO1	chr2	32168452	-	0.002413681	0.99758637
ENST00000345662	ENST00000404530	SPAST	chr2	32289315	+	MEMO1	chr2	32168452	-	0.002413681	0.99758637
ENST00000315285	ENST00000295065	SPAST	chr2	32289315	+	MEMO1	chr2	32168452	-	0.000306922	0.99969316
ENST00000315285	ENST00000379383	SPAST	chr2	32289315	+	MEMO1	chr2	32168452	-	0.001941019	0.998059
ENST00000315285	ENST00000404530	SPAST	chr2	32289315	+	MEMO1	chr2	32168452	-	0.001941019	0.998059
ENST00000345662	ENST00000295065	SPAST	chr2	32314674	+	MEMO1	chr2	32168452	-	0.00035515	0.9996449
ENST00000345662	ENST00000379383	SPAST	chr2	32314674	+	MEMO1	chr2	32168452	-	0.002919784	0.99708015
ENST00000345662	ENST00000404530	SPAST	chr2	32314674	+	MEMO1	chr2	32168452	-	0.002919784	0.99708015
ENST00000315285	ENST00000295065	SPAST	chr2	32314674	+	MEMO1	chr2	32168452	-	0.000345355	0.99965465
ENST00000315285	ENST00000379383	SPAST	chr2	32314674	+	MEMO1	chr2	32168452	-	0.002350655	0.9976494
ENST00000315285	ENST00000404530	SPAST	chr2	32314674	+	MEMO1	chr2	32168452	-	0.002350655	0.9976494

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>85531_85531_1_SPAST-MEMO1_SPAST_chr2_32289315_ENST00000315285_MEMO1_chr2_32168452_ENST00000295065_length(amino acids)=415AA_BP=138
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHA
YKQVDPSITRRIFILGPSHHVPLSRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFT
IIPVLVGALSESKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNT

--------------------------------------------------------------

>85531_85531_2_SPAST-MEMO1_SPAST_chr2_32289315_ENST00000315285_MEMO1_chr2_32168452_ENST00000379383_length(amino acids)=415AA_BP=138
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHA
YKQVDPSITRRIFILGPSHHVPLSRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFT
IIPVLVGALSESKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNT

--------------------------------------------------------------

>85531_85531_3_SPAST-MEMO1_SPAST_chr2_32289315_ENST00000315285_MEMO1_chr2_32168452_ENST00000404530_length(amino acids)=415AA_BP=138
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHA
YKQVDPSITRRIFILGPSHHVPLSRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFT
IIPVLVGALSESKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNT

--------------------------------------------------------------

>85531_85531_4_SPAST-MEMO1_SPAST_chr2_32289315_ENST00000345662_MEMO1_chr2_32168452_ENST00000295065_length(amino acids)=415AA_BP=138
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHA
YKQVDPSITRRIFILGPSHHVPLSRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFT
IIPVLVGALSESKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNT

--------------------------------------------------------------

>85531_85531_5_SPAST-MEMO1_SPAST_chr2_32289315_ENST00000345662_MEMO1_chr2_32168452_ENST00000379383_length(amino acids)=415AA_BP=138
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHA
YKQVDPSITRRIFILGPSHHVPLSRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFT
IIPVLVGALSESKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNT

--------------------------------------------------------------

>85531_85531_6_SPAST-MEMO1_SPAST_chr2_32289315_ENST00000345662_MEMO1_chr2_32168452_ENST00000404530_length(amino acids)=415AA_BP=138
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHA
YKQVDPSITRRIFILGPSHHVPLSRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFT
IIPVLVGALSESKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNT

--------------------------------------------------------------

>85531_85531_7_SPAST-MEMO1_SPAST_chr2_32314674_ENST00000315285_MEMO1_chr2_32168452_ENST00000295065_length(amino acids)=472AA_BP=195
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAK
MMTNLVMAKDRLQLLGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVDPSITRRIFILGPSHHVPLSRCALSSVDI
YRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFTIIPVLVGALSESKEQEFGKLFSKYLADPSNLFV
VSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLNYAQ

--------------------------------------------------------------

>85531_85531_8_SPAST-MEMO1_SPAST_chr2_32314674_ENST00000315285_MEMO1_chr2_32168452_ENST00000379383_length(amino acids)=472AA_BP=195
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAK
MMTNLVMAKDRLQLLGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVDPSITRRIFILGPSHHVPLSRCALSSVDI
YRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFTIIPVLVGALSESKEQEFGKLFSKYLADPSNLFV
VSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLNYAQ

--------------------------------------------------------------

>85531_85531_9_SPAST-MEMO1_SPAST_chr2_32314674_ENST00000315285_MEMO1_chr2_32168452_ENST00000404530_length(amino acids)=472AA_BP=195
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAK
MMTNLVMAKDRLQLLGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVDPSITRRIFILGPSHHVPLSRCALSSVDI
YRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFTIIPVLVGALSESKEQEFGKLFSKYLADPSNLFV
VSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLNYAQ

--------------------------------------------------------------

>85531_85531_10_SPAST-MEMO1_SPAST_chr2_32314674_ENST00000345662_MEMO1_chr2_32168452_ENST00000295065_length(amino acids)=472AA_BP=195
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAK
MMTNLVMAKDRLQLLGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVDPSITRRIFILGPSHHVPLSRCALSSVDI
YRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFTIIPVLVGALSESKEQEFGKLFSKYLADPSNLFV
VSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLNYAQ

--------------------------------------------------------------

>85531_85531_11_SPAST-MEMO1_SPAST_chr2_32314674_ENST00000345662_MEMO1_chr2_32168452_ENST00000379383_length(amino acids)=472AA_BP=195
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAK
MMTNLVMAKDRLQLLGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVDPSITRRIFILGPSHHVPLSRCALSSVDI
YRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFTIIPVLVGALSESKEQEFGKLFSKYLADPSNLFV
VSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLNYAQ

--------------------------------------------------------------

>85531_85531_12_SPAST-MEMO1_SPAST_chr2_32314674_ENST00000345662_MEMO1_chr2_32168452_ENST00000404530_length(amino acids)=472AA_BP=195
MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK
RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAK
MMTNLVMAKDRLQLLGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVDPSITRRIFILGPSHHVPLSRCALSSVDI
YRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFTIIPVLVGALSESKEQEFGKLFSKYLADPSNLFV
VSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLNYAQ

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:32289315/chr2:32168452)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
SPAST SPAST	MEMO1 Q9Y316
616	FUNCTION: May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration. {ECO:0000269\|PubMed:15156151, ECO:0000269\|PubMed:20937854}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

120_195

195.33333333333334

617.0

Domain

MIT

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

120_195

195.33333333333334

585.0

Domain

MIT

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

57_77

138.33333333333334

617.0

Intramembrane

Helical

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

57_77

138.33333333333334

585.0

Intramembrane

Helical

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

57_77

195.33333333333334

617.0

Intramembrane

Helical

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

57_77

195.33333333333334

585.0

Intramembrane

Helical

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

4_11

138.33333333333334

617.0

Motif

Nuclear localization signal

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

59_67

138.33333333333334

617.0

Motif

Nuclear export signal

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

4_11

138.33333333333334

585.0

Motif

Nuclear localization signal

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

59_67

138.33333333333334

585.0

Motif

Nuclear export signal

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

4_11

195.33333333333334

617.0

Motif

Nuclear localization signal

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

59_67

195.33333333333334

617.0

Motif

Nuclear export signal

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

4_11

195.33333333333334

585.0

Motif

Nuclear localization signal

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

59_67

195.33333333333334

585.0

Motif

Nuclear export signal

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

1_50

138.33333333333334

617.0

Region

Required for nuclear localization

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

1_50

138.33333333333334

585.0

Region

Required for nuclear localization

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

1_194

195.33333333333334

617.0

Region

Required for midbody localization

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

1_50

195.33333333333334

617.0

Region

Required for nuclear localization

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

1_194

195.33333333333334

585.0

Region

Required for midbody localization

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

1_50

195.33333333333334

585.0

Region

Required for nuclear localization

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

1_56

138.33333333333334

617.0

Topological domain

Cytoplasmic

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

1_56

138.33333333333334

585.0

Topological domain

Cytoplasmic

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

1_56

195.33333333333334

617.0

Topological domain

Cytoplasmic

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

1_56

195.33333333333334

585.0

Topological domain

Cytoplasmic

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

120_195

138.33333333333334

617.0

Domain

MIT

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

120_195

138.33333333333334

585.0

Domain

MIT

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

309_312

138.33333333333334

617.0

Motif

Nuclear localization signal

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

309_312

138.33333333333334

585.0

Motif

Nuclear localization signal

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

309_312

195.33333333333334

617.0

Motif

Nuclear localization signal

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

309_312

195.33333333333334

585.0

Motif

Nuclear localization signal

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

382_389

138.33333333333334

617.0

Nucleotide binding

ATP

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

382_389

138.33333333333334

585.0

Nucleotide binding

ATP

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

382_389

195.33333333333334

617.0

Nucleotide binding

ATP

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

382_389

195.33333333333334

585.0

Nucleotide binding

ATP

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

1_194

138.33333333333334

617.0

Region

Required for midbody localization

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

228_616

138.33333333333334

617.0

Region

Sufficient for microtubule severing

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

1_194

138.33333333333334

585.0

Region

Required for midbody localization

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

228_616

138.33333333333334

585.0

Region

Sufficient for microtubule severing

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

228_616

195.33333333333334

617.0

Region

Sufficient for microtubule severing

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

228_616

195.33333333333334

585.0

Region

Sufficient for microtubule severing

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000315285

78_616

138.33333333333334

617.0

Topological domain

Cytoplasmic

Hgene

SPAST

chr2:32289315

chr2:32168452

ENST00000345662

78_616

138.33333333333334

585.0

Topological domain

Cytoplasmic

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000315285

78_616

195.33333333333334

617.0

Topological domain

Cytoplasmic

Hgene

SPAST

chr2:32314674

chr2:32168452

ENST00000345662

78_616

195.33333333333334

585.0

Topological domain

Cytoplasmic

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>853_SPAST_32289315_MEMO1_32168452_853_SPAST_32289315_MEMO1_32168452_ranked_0.pdb	SPAST	32314674	32289315	ENST00000404530	MEMO1	chr2	32168452	-	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAK RSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHA YKQVDPSITRRIFILGPSHHVPLSRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDEFT IIPVLVGALSESKEQEFGKLFSKYLADPSNLFVVSSDFCHWGQRFRYSYYDESQGEIYRSIEHLDKMGMSIIEQLDPVSFSNYLKKYHNT	415

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

SPAST_pLDDT.png

MEMO1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
SPAST
MEMO1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to SPAST-MEMO1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to SPAST-MEMO1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source