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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:TRMT10B-AR

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: TRMT10B-AR
	FusionPDB ID: 94274
	FusionGDB2.0 ID: 94274
		Hgene	Tgene
	Gene symbol	TRMT10B	AR
	Gene ID	158234	374
	Gene name	tRNA methyltransferase 10B	amphiregulin
	Synonyms	RG9MTD3\|bA3J10.9	AR\|AREGB\|CRDGF\|SDGF
	Cytomap	9p13.2	4q13.3
	Type of gene	protein-coding	protein-coding
	Description	tRNA methyltransferase 10 homolog BRNA (guanine-9-) methyltransferase domain containing 3RNA (guanine-9-)-methyltransferase domain-containing protein 3tRNA (guanine(9)-N(1))-methyltransferase TRMT10B	amphiregulinamphiregulin Bcolorectum cell-derived growth factorschwannoma-derived growth factor
	Modification date	20200313	20200327
	UniProtAcc	.	ARSK
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000297994, ENST00000377753, ENST00000377754, ENST00000537911,	ENST00000396043, ENST00000396044, ENST00000504326, ENST00000513847, ENST00000374690,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	2 X 3 X 2=12	14 X 10 X 11=1540
	# samples	3	16
	** MAII score	log2(3/12*10)=1.32192809488736 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0	log2(16/1540*10)=-3.2667865406949 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: TRMT10B [Title/Abstract] AND AR [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	TRMT10B(37776402)-AR(66905852), # samples:2
Anticipated loss of major functional domain due to fusion event.	TRMT10B-AR seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TRMT10B-AR seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TRMT10B-AR seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. TRMT10B-AR seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	AR	GO:0007173	epidermal growth factor receptor signaling pathway	12743035
Tgene	AR	GO:0007186	G protein-coupled receptor signaling pathway	12743035

Fusion gene breakpoints across TRMT10B (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across AR (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BRCA	TCGA-A8-A08I-01A	TRMT10B	chr9	37776402	+	AR	chrX	66905852	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000377753

TRMT10B

chr9

37776402

ENST00000374690

chrX

66905852

8458

685

1679

534

ENST00000537911

TRMT10B

chr9

37776402

ENST00000374690

chrX

66905852

8533

760

1754

561

ENST00000377754

TRMT10B

chr9

37776402

ENST00000374690

chrX

66905852

8573

800

241

1794

517

ENST00000297994

TRMT10B

chr9

37776402

ENST00000374690

chrX

66905852

8682

909

1903

612

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000377753	ENST00000374690	TRMT10B	chr9	37776402	+	AR	chrX	66905852	+	0.000330541	0.99966943
ENST00000537911	ENST00000374690	TRMT10B	chr9	37776402	+	AR	chrX	66905852	+	0.000265969	0.999734
ENST00000377754	ENST00000374690	TRMT10B	chr9	37776402	+	AR	chrX	66905852	+	0.000561449	0.9994386
ENST00000297994	ENST00000374690	TRMT10B	chr9	37776402	+	AR	chrX	66905852	+	0.000257072	0.9997429

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>94274_94274_1_TRMT10B-AR_TRMT10B_chr9_37776402_ENST00000297994_AR_chrX_66905852_ENST00000374690_length(amino acids)=612AA_BP=281
MDWKLEGSTQKVESPVLQGQEGILEETGEDGLPEGFQLLQIDAEGECQEGEILATGSTAWCSKNVQRKQRHWEKIVAAKKSKRKQEKERR
KANRAENPGICPQHSKRFLRALTKDKLLEAKHSGPRLCIDLSMTHYMSKKELSRLAGQIRRLYGSNKKADRPFWICLTGFTTDSPLYEEC
VRMNDGFSSYLLDITEEDCFSLFPLETLVYLTPDSEHALEDVDLNKVYILGGLVDESIQKKVTFQKAREYSVKTARLPIQEYMVRNQNGK
NYHSEILAINQGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGY
ECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNV
NSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRK

--------------------------------------------------------------

>94274_94274_2_TRMT10B-AR_TRMT10B_chr9_37776402_ENST00000377753_AR_chrX_66905852_ENST00000374690_length(amino acids)=534AA_BP=203
MDWKLEGSTQKVESPVLQGQEGILEETGEDGLPEGFQLLQIDAEGECQEGEILATGSTAWCSELSRLAGQIRRLYGSNKKADRPFWICLT
GFTTDSPLYEECVRMNDGFSSYLLDITEEDCFSLFPLETLVYLTPDSEHALEDVDLNKVYILGGLVDESIQKKVTFQKAREYSVKTARLP
IQEYMVRNQNGKNYHSEILAINQGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEET
TQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLM
VFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYI

--------------------------------------------------------------

>94274_94274_3_TRMT10B-AR_TRMT10B_chr9_37776402_ENST00000377754_AR_chrX_66905852_ENST00000374690_length(amino acids)=517AA_BP=186
MVLGICPQHSKRFLRALTKDKLLEAKHSGPRLCIDLSMTHYMSKKELSRLAGQIRRLYGSNKKADRPFWICLTGFTTDSPLYEECVRMND
GFSSYLLDITEEDCFSLFPLETLVYLTPDSEHALEDVDLNKVYILGGLVDESIQKKVTFQKAREYSVKTARLPIQEYMVRNQNGKNYHSE
ILAINQGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPI
FLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRML
YFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSC

--------------------------------------------------------------

>94274_94274_4_TRMT10B-AR_TRMT10B_chr9_37776402_ENST00000537911_AR_chrX_66905852_ENST00000374690_length(amino acids)=561AA_BP=230
MDWKLEGSTQKVESPVLQGQEGILEETGEDGLPEGFQLLQIDAEGECQEGEILATGSTAWCSKNVQRKQRHWEKIVAAKKSKRKQEKERR
KANRAENPGICPQHSKRFLRALTKDKLLEAKHSGPRLCIDLSMTHYMSKKLDITEEDCFSLFPLETLVYLTPDSEHALEDVDLNKVYILG
GLVDESIQKKVTFQKAREYSVKTARLPIQEYMVRNQNGKNYHSEILAINQGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGA
RKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKW
AKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMK
ALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEI

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr9:37776402/chrX:66905852)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	AR ARSK
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	536

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TRMT10B

chr9:37776402

chrX:66905852

ENST00000297994

73_97

281.3333333333333

317.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

TRMT10B

chr9:37776402

chrX:66905852

ENST00000377754

73_97

186.33333333333334

222.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

195_199

57.333333333333336

389.0

Compositional bias

Note=Poly-Gln

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

374_383

57.333333333333336

389.0

Compositional bias

Note=Poly-Pro

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

398_404

57.333333333333336

389.0

Compositional bias

Note=Poly-Ala

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

451_473

57.333333333333336

389.0

Compositional bias

Note=Poly-Gly

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

58_120

57.333333333333336

389.0

Compositional bias

Note=Gln-rich

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

58_80

57.333333333333336

389.0

Compositional bias

Note=Poly-Gln

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

86_91

57.333333333333336

389.0

Compositional bias

Note=Poly-Gln

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

560_632

57.333333333333336

389.0

DNA binding

Nuclear receptor

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

669_900

57.333333333333336

389.0

Domain

NR LBD

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

560_580

57.333333333333336

389.0

Zinc finger

NR C4-type

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

596_620

57.333333333333336

389.0

Zinc finger

NR C4-type

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TRMT10B

chr9:37776402

chrX:66905852

ENST00000297994

113_310

281.3333333333333

317.0

Domain

SAM-dependent MTase TRM10-type

Hgene

TRMT10B

chr9:37776402

chrX:66905852

ENST00000377754

113_310

186.33333333333334

222.0

Domain

SAM-dependent MTase TRM10-type

Tgene

chr9:37776402

chrX:66905852

ENST00000396043

1_559

57.333333333333336

389.0

Region

Note=Modulating

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
TRMT10B
AR

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	AR	chr9:37776402	chrX:66905852	ENST00000396043		1	8	1_587	57.333333333333336	389.0	ZNF318

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Related Drugs to TRMT10B-AR

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to TRMT10B-AR

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source